For about half a century, chemical control has played a major role in plant disease control. However, the long-term irrational use of chemical pesticide produces many problems. In nature, there exit extensive antagoni...For about half a century, chemical control has played a major role in plant disease control. However, the long-term irrational use of chemical pesticide produces many problems. In nature, there exit extensive antagonistic microorganisms which are tightly concerned with plant pathogenic microbes, and biological pesticides can be researched to control related pathogenic microbes from its metabolites. It＇s an important research direction of new pesticide development. The Bacillus is the ideal and frequently studied object of bio-control bacteria, and it can produce some entospores with following characteristics such as heat-resistant, drought tolerance, antiultraviolet and organic solvent. In this article, the bio-control mechanism, problems and application prospects of the Bacillus were reviewed to promote the application in new biological pesticide.展开更多
[ Objective] The paper was to identify endophytic bacillus BHL3501 from wild soybean and its inhibitory effect against soybean cyst nematode. [ Meth- od] Through morphological characteristic, physiological and biochem...[ Objective] The paper was to identify endophytic bacillus BHL3501 from wild soybean and its inhibitory effect against soybean cyst nematode. [ Meth- od] Through morphological characteristic, physiological and biochemical characteristics and 16S rDNA analysis, a strain of endophytic bacterium BHL3501 with ne- maticidal activity isolated and screened from the root of wild soybean was identified, the effects of BHL3501 metabolite solutions with different dilution multiples on egg hatching and activity of 2rd instar juvenile of soybean cyst nematode were also studied. [ Result ] BHL3501 strain was preliminarily identified to be Bacillus sp.. The relative inhibitory rate of its original fermentation broth on egg hatching of soybean cyst nematode after 24 h was 98.3% ; the relative inhibitory rate under 10- time diluted solution treatment was 79.5%, which had significant difference with sterile water control. The corrected mortality rate of 24 instar juvenile was 93.1% after treated by original fermentation broth for 24 h, the treatments of all diluted solutions had significant difference with sterile water control. [ Conclusion ] BHL3501 metabolites had strong inhibitory effect against egg hatching of soybean cyst nematode, which also had strong toxic effect on its 2nd instar juvenile.展开更多
基金Supported by Accelerated Program of Sichuan Academy of Agricultural Sciences(2013QNJJ-019)Spark Program of Ministry of Science and Technology(2011GA-810011)Special Program of Modern Agricultural Technological System(No.CARS-22)~~
文摘For about half a century, chemical control has played a major role in plant disease control. However, the long-term irrational use of chemical pesticide produces many problems. In nature, there exit extensive antagonistic microorganisms which are tightly concerned with plant pathogenic microbes, and biological pesticides can be researched to control related pathogenic microbes from its metabolites. It＇s an important research direction of new pesticide development. The Bacillus is the ideal and frequently studied object of bio-control bacteria, and it can produce some entospores with following characteristics such as heat-resistant, drought tolerance, antiultraviolet and organic solvent. In this article, the bio-control mechanism, problems and application prospects of the Bacillus were reviewed to promote the application in new biological pesticide.
基金Supported by National Natural Science Foundation of China ( 30940050)Natural Science Foundation of Hebei Province ( C2006000559+2 种基金 C2009000868) Commission of Science Technology of Hebei Province ( 06547005D-3,09220103D-11)Educational Commission of Hebei Province( Z2008114)~~
文摘[ Objective] The paper was to identify endophytic bacillus BHL3501 from wild soybean and its inhibitory effect against soybean cyst nematode. [ Meth- od] Through morphological characteristic, physiological and biochemical characteristics and 16S rDNA analysis, a strain of endophytic bacterium BHL3501 with ne- maticidal activity isolated and screened from the root of wild soybean was identified, the effects of BHL3501 metabolite solutions with different dilution multiples on egg hatching and activity of 2rd instar juvenile of soybean cyst nematode were also studied. [ Result ] BHL3501 strain was preliminarily identified to be Bacillus sp.. The relative inhibitory rate of its original fermentation broth on egg hatching of soybean cyst nematode after 24 h was 98.3% ; the relative inhibitory rate under 10- time diluted solution treatment was 79.5%, which had significant difference with sterile water control. The corrected mortality rate of 24 instar juvenile was 93.1% after treated by original fermentation broth for 24 h, the treatments of all diluted solutions had significant difference with sterile water control. [ Conclusion ] BHL3501 metabolites had strong inhibitory effect against egg hatching of soybean cyst nematode, which also had strong toxic effect on its 2nd instar juvenile.
