Bacillus subtilis HJX1, a biocontrol strain of Fusarium oxysporum f. sp. cubense, could inhibit growth of several plant pathogenic fungi in vitro. To un- derstand its mechanism of fungistasis, we extracted crude prote...Bacillus subtilis HJX1, a biocontrol strain of Fusarium oxysporum f. sp. cubense, could inhibit growth of several plant pathogenic fungi in vitro. To un- derstand its mechanism of fungistasis, we extracted crude protein from fermentation broth of the strain HJX1 through ammonium sulfate precipitation, and prelimina- rily studied fungistatic activities at different conditions. The results showed that the crude protein was insensitive to protease K, trypsin or ultraviolet radiation. The fungistatic activity was unchanged when treated in water batch at 40 ℃, 60 ℃, 80 ℃ or 100 ℃ for 30 min, and the fungistatic activity maintained 60% when trea- ted at 121℃ for 30 min.展开更多
A biocontrol strain HJX1,which had good inhibitory effect against Fusarium oxysporum f. sp. cubense,was identified as Bacillus subtilis based on traditional morphology,16 S rRNA gene sequence and fatty acid profiles. ...A biocontrol strain HJX1,which had good inhibitory effect against Fusarium oxysporum f. sp. cubense,was identified as Bacillus subtilis based on traditional morphology,16 S rRNA gene sequence and fatty acid profiles. The sequence of 16 S rRNA gene had 99% homology to B. subtilis according to the DNA fragment of 1 521 bp amplified with a pair of universal primers,P0 and P6,of bacteria. Confront culture with pathogenic fungi showed that this strain had good inhibitory effect against 10 pathogenic fungi.展开更多
基金Supported by the natural science foundation of Hainan Province in 2015(20158304)
文摘Bacillus subtilis HJX1, a biocontrol strain of Fusarium oxysporum f. sp. cubense, could inhibit growth of several plant pathogenic fungi in vitro. To un- derstand its mechanism of fungistasis, we extracted crude protein from fermentation broth of the strain HJX1 through ammonium sulfate precipitation, and prelimina- rily studied fungistatic activities at different conditions. The results showed that the crude protein was insensitive to protease K, trypsin or ultraviolet radiation. The fungistatic activity was unchanged when treated in water batch at 40 ℃, 60 ℃, 80 ℃ or 100 ℃ for 30 min, and the fungistatic activity maintained 60% when trea- ted at 121℃ for 30 min.
基金Supported by Culture Fund of Hainan Medical College for Scientific Researchin 2013(HY2013-22)
文摘A biocontrol strain HJX1,which had good inhibitory effect against Fusarium oxysporum f. sp. cubense,was identified as Bacillus subtilis based on traditional morphology,16 S rRNA gene sequence and fatty acid profiles. The sequence of 16 S rRNA gene had 99% homology to B. subtilis according to the DNA fragment of 1 521 bp amplified with a pair of universal primers,P0 and P6,of bacteria. Confront culture with pathogenic fungi showed that this strain had good inhibitory effect against 10 pathogenic fungi.
