Bacillus velezensis is a Gram-positive and spore-forming bacterium.It has potent antimicrobial properties that can be used to promote plant growth and as a pesticide by inhibiting pathogens.B.velezensis has the capabi...Bacillus velezensis is a Gram-positive and spore-forming bacterium.It has potent antimicrobial properties that can be used to promote plant growth and as a pesticide by inhibiting pathogens.B.velezensis has the capability to generate a diverse range of enzymes that have potential applications in various fields,such as enzyme production,fermented food,degradation of pollutants,and bioenergy.In addition,B.velezensis is a promising probiotic.It possesses high bile-salt tolerance characteristics and has a high success rate of colonization in the intestinal mucosa.Besides,the strain can also regulate gut microbiota constitute by increasing the number of beneficial microorganisms and decreasing the number of pathogens.Furthermore,based on its special properties,including high-yield protease production and high salt-tolerance,B.velezensis shows potential for use in marine protein fermentation,opening up new avenues for the development of novel food products and bioactive peptides.In addition,B.velezensis can shorten the fermentation time as well as improve the nutritional value and flavor of fermented food.The safety of B.velezensis for food production was evaluated.This review provides valuable insights into the potential uses and benefits of B.velezensis,particularly in the context of fermented foods.展开更多
Crown gall of Prunus subhirtella Miq., flowering cherry, is a soil-borne bacterial disease caused by Agrobacterium tumefaciens Smith & Townsend and has caused serious damage to the species in recent years in China...Crown gall of Prunus subhirtella Miq., flowering cherry, is a soil-borne bacterial disease caused by Agrobacterium tumefaciens Smith & Townsend and has caused serious damage to the species in recent years in China. In this study, the colonization of sapling roots, the biocontrol efficacy, the expression of defense-related genes and the enzyme activity in roots of P. subhirtella inoculated with the Bacillus velezensis JK-XZ8 against A. tumefaciens were determined under greenhouse conditions. The results showed that the JK-XZ8 strain colonized in the rhizosphere and root surfaces of the saplings. Sole application of the strain increased activities of polyphenol oxidase (PPO) and phenylalanine ammonia lyase (PAL) without affecting superoxide dismutase (SOD). SOD and PPO activities increased in the prevention group, and the activity of PAL increased in both the prevention and cure groups. The expression of the defense-related genes PAL and PR-1 of salicylic acid pathway in roots increased in both the prevention and cure groups. Simultaneously on day 3, the expression of both COI and MYC2 genes of the jasmonic acid pathway significantly increased in the two groups. The incidence of crown gall in the prevention and the cure groups were 48.1% and 66.7%, respectively, significantly lower compared to the pathogens alone (87.5%). This study showed that the application of the JK-XZ8 strain reduced the incidence of crown gall in P. subhirtella saplings in the two groups, and the prevention group had better control efficacy. In addition, the JK-XZ8 strain protects against crown gall by developing induced systemic resistance and systemic acquired resistance in the roots of the cherry saplings.展开更多
Bacillus velezensis M3-1 strain isolated from the sediment of Myriophyllum aquatium con-structedwetlandswas found to efficiently convert NO_(3)^(-)-N to NO_(2)^(−)-N,and the requirements for carbon source additionwere...Bacillus velezensis M3-1 strain isolated from the sediment of Myriophyllum aquatium con-structedwetlandswas found to efficiently convert NO_(3)^(-)-N to NO_(2)^(−)-N,and the requirements for carbon source additionwere not very rigorous.Thiswork demonstrates,for the first time,the feasibility of using the synergy of anammox and Bacillus velezensis M3-1 microorganisms for nitrogen removal.In this study,the possibility of M3-1 that converted NO_(3)^(−)-N produced by anammox to NO_(2)^(−)-N was verified in an anaerobic reactor.The NO_(3)^(−)-N reduction ability of M3-1 and denitrifying bacteria in coupling system was investigated under different C/N conditions,and it was found that M3-1 used carbon sources preferentially over denitrifying bacteria.By adjusting the ratio of NH4+-N to NO_(2)^(−)-N,it was found that the NO_(2)^(−)-N con-verted from NO_(3)^(−)-N by M3-1 participated in the original anammox.The nitrogen removal efficacy(NRE)of the coupled system was increased by 12.1%,compared to the control group anammox system at C/N=2:1.Functional gene indicated that itmight be a nitrate reducing bacterium.This study shows that the nitrate reduction rate achieved by the Bacillus velezensis M3-1 can be high enough for removing nitrate produced by anammox process,which would enable improve nitrogen removal from wastewater.展开更多
This study was to evaluate the potential of a host-associated Bacillus velezensis as a probiotic for hybrid yellow catfish(Pelteobagrus fulvidraco♀×Pelteobagrus vachelli♂).Diets(B0 to B5)containing 0,0.90×...This study was to evaluate the potential of a host-associated Bacillus velezensis as a probiotic for hybrid yellow catfish(Pelteobagrus fulvidraco♀×Pelteobagrus vachelli♂).Diets(B0 to B5)containing 0,0.90×10^(8),0.80×109,0.85×10^(10),0.90×10^(11),0.83×10^(12)CFU/kg B.velezensis YFI-E109 were fed to the fish with initial weight(3.07±0.08 g)in a recirculating aquaculture system for six weeks with three replicates,respectively.Probiotic effects were analyzed based on growth,body composition,liver and gut morphology,gut microbiome,and liver metabolome.Analysis of the bacterial genome has shown that the most abundant genes in B.velezensis YFI-E109 were distributed in carbohydrate and amino acid metabolism.Fish in groups B3 and B4 had better growth performance,and higher intestinal amylase(AMS)and lipase(LPS)activities compared with other groups(P<0.05).Fish in groups B0 and B5 showed significant liver damage,while this status improved in group B3.The liver malondialdehyde(MDA)content in group B3 was lower than that in other groups(P<0.05).The abundance of Mycoplasma,Ralstonia and Acinetobacter was significantly reduced in B3 and B5 compared to B0.The amino acid and carbohydrate metabolism pathways were enriched in group B3 compared with group B0.In conclusion,dietary B.velezensis YFI-E109 supplementation has the potential to improve growth,liver metabolism,and liver and gut health,and reshape the gut microbiome of hybrid yellow catfish.Excessive B.velezensis YFI-E109 reduced the prebiotic effects.The recommended dietary supplementation of B.velezensis YFI-E109 is 0.31×10^(10) to 0.77×10^(11)CFU/kg for hybrid yellow catfish according to the quadratic regression method by plotting specific growth rate(SGR),feed conversion ratio(FCR),MDA and activities of AMS against dietary B.velezensis YFI-E109 levels.展开更多
For a safe and sustainable environment,effective microbes as biocontrol agents are in high demand.We have isolated a new Bacillus velezensis strain DTU001,investigated its antifungal spectrum,sequenced its genome,and ...For a safe and sustainable environment,effective microbes as biocontrol agents are in high demand.We have isolated a new Bacillus velezensis strain DTU001,investigated its antifungal spectrum,sequenced its genome,and uncovered the production of lipopeptides in HPLC-HRMS analysis.To test the antifungal efficacy,extracts of B.velezensis DTU001 was tested against a range of twenty human or plant pathogenic fungi.We demonstrate that inhibitory potential of B.velezensis DTU001 against selected fungi is superior in comparison to single lipopeptide,either iturin or fengycin.The isolate showed analogous biofilm formation to other closely related Bacilli.To further support the biocontrol properties of the isolate,coculture with Candida albicans demonstrated that B.velezensis DTU001 exhibited excellent antiproliferation effect against C.albicans.In summary,the described isolate is a potential antifungal agent with a broad antifungal spectrum that might assist our aims to avoid hazardous pathogenic fungi and provide alternative to toxicity caused by chemicals.展开更多
A novel glutamate-dependent poly-γ-glutamic acid(γ-PGA)-producing strain Bacillus velezensis CAU263 isolated from Chinese traditional Douchi was evaluated.An efficient method ofγ-PGA production was performed by thi...A novel glutamate-dependent poly-γ-glutamic acid(γ-PGA)-producing strain Bacillus velezensis CAU263 isolated from Chinese traditional Douchi was evaluated.An efficient method ofγ-PGA production was performed by this strain via solid-state fermentation(SSF)using guar meal.The maximal yield ofγ-PGA reached 158.5 g/kg dry weight(DW)in 250 mL flasks and 155.1 g/kg DW in shallow tray,respectively.The molecular weight ofγ-PGA was 3.8×106 Da,the ratio of L/D-glutamic acid was 26.1%and 73.9%.Additionally,γ-PGA significantly improved the operating characteristics of wheat dough,the specific volume of bread was increased by 15.8%and the bread hardness was reduced by 44.2%owing to the addition of 0.3 g/kgγ-PGA.Thus,γ-PGA could be produced by Bacillus velezensis CAU263 at high level using guar meal and might have potential application prospect in food industry.展开更多
With the rapid expansion of livestock production,the amount of livestock wastewater accumulated rapidly.Lack of biodegradable organic matter makes denitrification of livestock wastewater after anaerobic digestion more...With the rapid expansion of livestock production,the amount of livestock wastewater accumulated rapidly.Lack of biodegradable organic matter makes denitrification of livestock wastewater after anaerobic digestion more difficult.In this study,Myriophyllum aquaticum constructed wetlands(CWs)with efficient nitrogen removal performance were established under different carbon/nitrogen(C/N)ratios.Analysis of community composition reveals the change of M.aquaticum CWs in microbial community structure with C/N ratios.The proportion of Proteobacteria which is one of the dominant phyla among denitrifier communities increased significantly under low C/N ratio conditions.Besides,to obtain cultivable denitrifier that could be added into CWs in situ,33 strains belonging to phylum Proteobacteria were isolated from efficient M.aquaticum CWs,while the best-performing denitrification strain M3-1 was identified as Bacillus velezensis JT3-1(GenBank No.CP032506.1).Redundancy analysis and quadratic models showed that C/N ratio had significant effects on disposal of nitrate(NO_(3)^(−)-N)and the strains isolated could perform well in denitrification when C/N ratio is relatively low.In addition,they have relatively wide ranges of carbon sources,temperature and a high NO_(3)^(−)removal rate of 9.12 mg/(L·hr)at elevated concentrations of 800 mg/L nitrate.Thus,strains isolated from M.aquaticum CWs with low C/N ratio have a practical application value in the treatment of nitrate-containing wastewater.These denitrifying bacteria could be added to CWs to enhance nitrogen removal efficiency of CWs for livestock wastewater with low C/N ratio in the future.展开更多
基金financially supported by the Public Welfare Project of Zhoushan City,Zhejiang(2022C31050)。
文摘Bacillus velezensis is a Gram-positive and spore-forming bacterium.It has potent antimicrobial properties that can be used to promote plant growth and as a pesticide by inhibiting pathogens.B.velezensis has the capability to generate a diverse range of enzymes that have potential applications in various fields,such as enzyme production,fermented food,degradation of pollutants,and bioenergy.In addition,B.velezensis is a promising probiotic.It possesses high bile-salt tolerance characteristics and has a high success rate of colonization in the intestinal mucosa.Besides,the strain can also regulate gut microbiota constitute by increasing the number of beneficial microorganisms and decreasing the number of pathogens.Furthermore,based on its special properties,including high-yield protease production and high salt-tolerance,B.velezensis shows potential for use in marine protein fermentation,opening up new avenues for the development of novel food products and bioactive peptides.In addition,B.velezensis can shorten the fermentation time as well as improve the nutritional value and flavor of fermented food.The safety of B.velezensis for food production was evaluated.This review provides valuable insights into the potential uses and benefits of B.velezensis,particularly in the context of fermented foods.
基金This work was supported by the National Key Research and Development Program of China(2017YFD0600104)the Priority Academic Program Development of the Jiangsu Higher Education Institutions(PAPD)。
文摘Crown gall of Prunus subhirtella Miq., flowering cherry, is a soil-borne bacterial disease caused by Agrobacterium tumefaciens Smith & Townsend and has caused serious damage to the species in recent years in China. In this study, the colonization of sapling roots, the biocontrol efficacy, the expression of defense-related genes and the enzyme activity in roots of P. subhirtella inoculated with the Bacillus velezensis JK-XZ8 against A. tumefaciens were determined under greenhouse conditions. The results showed that the JK-XZ8 strain colonized in the rhizosphere and root surfaces of the saplings. Sole application of the strain increased activities of polyphenol oxidase (PPO) and phenylalanine ammonia lyase (PAL) without affecting superoxide dismutase (SOD). SOD and PPO activities increased in the prevention group, and the activity of PAL increased in both the prevention and cure groups. The expression of the defense-related genes PAL and PR-1 of salicylic acid pathway in roots increased in both the prevention and cure groups. Simultaneously on day 3, the expression of both COI and MYC2 genes of the jasmonic acid pathway significantly increased in the two groups. The incidence of crown gall in the prevention and the cure groups were 48.1% and 66.7%, respectively, significantly lower compared to the pathogens alone (87.5%). This study showed that the application of the JK-XZ8 strain reduced the incidence of crown gall in P. subhirtella saplings in the two groups, and the prevention group had better control efficacy. In addition, the JK-XZ8 strain protects against crown gall by developing induced systemic resistance and systemic acquired resistance in the roots of the cherry saplings.
基金supported by the National Natural Sci-ence Foundation of China(Nos.42177099,91951108,and 21976197)the Knowledge Innovation Program of Shenzhen(No.JSGG20191129112812329),and the CAS International Part-nership Program(No.121311KYSB20200017)the special fund of State Key Joint Laboratory of Environment Simula-tion and Pollution Control(No.21Z02ESPCR).
文摘Bacillus velezensis M3-1 strain isolated from the sediment of Myriophyllum aquatium con-structedwetlandswas found to efficiently convert NO_(3)^(-)-N to NO_(2)^(−)-N,and the requirements for carbon source additionwere not very rigorous.Thiswork demonstrates,for the first time,the feasibility of using the synergy of anammox and Bacillus velezensis M3-1 microorganisms for nitrogen removal.In this study,the possibility of M3-1 that converted NO_(3)^(−)-N produced by anammox to NO_(2)^(−)-N was verified in an anaerobic reactor.The NO_(3)^(−)-N reduction ability of M3-1 and denitrifying bacteria in coupling system was investigated under different C/N conditions,and it was found that M3-1 used carbon sources preferentially over denitrifying bacteria.By adjusting the ratio of NH4+-N to NO_(2)^(−)-N,it was found that the NO_(2)^(−)-N con-verted from NO_(3)^(−)-N by M3-1 participated in the original anammox.The nitrogen removal efficacy(NRE)of the coupled system was increased by 12.1%,compared to the control group anammox system at C/N=2:1.Functional gene indicated that itmight be a nitrate reducing bacterium.This study shows that the nitrate reduction rate achieved by the Bacillus velezensis M3-1 can be high enough for removing nitrate produced by anammox process,which would enable improve nitrogen removal from wastewater.
基金supported by the National Key R&D Program of China(Grant No.2022YFD2400902)China Agricultural Research System(Grant/Award Number:CARS-46)the Fund of Fujian Key Laboratory of Functional Aquafeed and Culture Environment Control(FACE20200004).
文摘This study was to evaluate the potential of a host-associated Bacillus velezensis as a probiotic for hybrid yellow catfish(Pelteobagrus fulvidraco♀×Pelteobagrus vachelli♂).Diets(B0 to B5)containing 0,0.90×10^(8),0.80×109,0.85×10^(10),0.90×10^(11),0.83×10^(12)CFU/kg B.velezensis YFI-E109 were fed to the fish with initial weight(3.07±0.08 g)in a recirculating aquaculture system for six weeks with three replicates,respectively.Probiotic effects were analyzed based on growth,body composition,liver and gut morphology,gut microbiome,and liver metabolome.Analysis of the bacterial genome has shown that the most abundant genes in B.velezensis YFI-E109 were distributed in carbohydrate and amino acid metabolism.Fish in groups B3 and B4 had better growth performance,and higher intestinal amylase(AMS)and lipase(LPS)activities compared with other groups(P<0.05).Fish in groups B0 and B5 showed significant liver damage,while this status improved in group B3.The liver malondialdehyde(MDA)content in group B3 was lower than that in other groups(P<0.05).The abundance of Mycoplasma,Ralstonia and Acinetobacter was significantly reduced in B3 and B5 compared to B0.The amino acid and carbohydrate metabolism pathways were enriched in group B3 compared with group B0.In conclusion,dietary B.velezensis YFI-E109 supplementation has the potential to improve growth,liver metabolism,and liver and gut health,and reshape the gut microbiome of hybrid yellow catfish.Excessive B.velezensis YFI-E109 reduced the prebiotic effects.The recommended dietary supplementation of B.velezensis YFI-E109 is 0.31×10^(10) to 0.77×10^(11)CFU/kg for hybrid yellow catfish according to the quadratic regression method by plotting specific growth rate(SGR),feed conversion ratio(FCR),MDA and activities of AMS against dietary B.velezensis YFI-E109 levels.
基金This work was supported by the Danish National Research Foundation(DNRF137)for the Center for Microbial Secondary Metabolites(CeMiSt)SD was supported by a Novozymes and Henning Holck Larsen fellowship during her stay at DTU.TW was supported by a grant from Novo Nordisk Foundation(NNF10CC1016517).
文摘For a safe and sustainable environment,effective microbes as biocontrol agents are in high demand.We have isolated a new Bacillus velezensis strain DTU001,investigated its antifungal spectrum,sequenced its genome,and uncovered the production of lipopeptides in HPLC-HRMS analysis.To test the antifungal efficacy,extracts of B.velezensis DTU001 was tested against a range of twenty human or plant pathogenic fungi.We demonstrate that inhibitory potential of B.velezensis DTU001 against selected fungi is superior in comparison to single lipopeptide,either iturin or fengycin.The isolate showed analogous biofilm formation to other closely related Bacilli.To further support the biocontrol properties of the isolate,coculture with Candida albicans demonstrated that B.velezensis DTU001 exhibited excellent antiproliferation effect against C.albicans.In summary,the described isolate is a potential antifungal agent with a broad antifungal spectrum that might assist our aims to avoid hazardous pathogenic fungi and provide alternative to toxicity caused by chemicals.
基金This study was funded by the 13th national five-year plan for Key Research and Development of China“modern food processing and food storage and transportation technology and equipment”(No.2018YFD0400404).
文摘A novel glutamate-dependent poly-γ-glutamic acid(γ-PGA)-producing strain Bacillus velezensis CAU263 isolated from Chinese traditional Douchi was evaluated.An efficient method ofγ-PGA production was performed by this strain via solid-state fermentation(SSF)using guar meal.The maximal yield ofγ-PGA reached 158.5 g/kg dry weight(DW)in 250 mL flasks and 155.1 g/kg DW in shallow tray,respectively.The molecular weight ofγ-PGA was 3.8×106 Da,the ratio of L/D-glutamic acid was 26.1%and 73.9%.Additionally,γ-PGA significantly improved the operating characteristics of wheat dough,the specific volume of bread was increased by 15.8%and the bread hardness was reduced by 44.2%owing to the addition of 0.3 g/kgγ-PGA.Thus,γ-PGA could be produced by Bacillus velezensis CAU263 at high level using guar meal and might have potential application prospect in food industry.
基金supported by the National Natural Science Foundation of China(Nos.42177099 and 91951108)the Key R&D plan of Ningxia Hui Autonomous Region(No.2019BFG02032)the CAS International Partnership Program(No.121311KYSB20200017).
文摘With the rapid expansion of livestock production,the amount of livestock wastewater accumulated rapidly.Lack of biodegradable organic matter makes denitrification of livestock wastewater after anaerobic digestion more difficult.In this study,Myriophyllum aquaticum constructed wetlands(CWs)with efficient nitrogen removal performance were established under different carbon/nitrogen(C/N)ratios.Analysis of community composition reveals the change of M.aquaticum CWs in microbial community structure with C/N ratios.The proportion of Proteobacteria which is one of the dominant phyla among denitrifier communities increased significantly under low C/N ratio conditions.Besides,to obtain cultivable denitrifier that could be added into CWs in situ,33 strains belonging to phylum Proteobacteria were isolated from efficient M.aquaticum CWs,while the best-performing denitrification strain M3-1 was identified as Bacillus velezensis JT3-1(GenBank No.CP032506.1).Redundancy analysis and quadratic models showed that C/N ratio had significant effects on disposal of nitrate(NO_(3)^(−)-N)and the strains isolated could perform well in denitrification when C/N ratio is relatively low.In addition,they have relatively wide ranges of carbon sources,temperature and a high NO_(3)^(−)removal rate of 9.12 mg/(L·hr)at elevated concentrations of 800 mg/L nitrate.Thus,strains isolated from M.aquaticum CWs with low C/N ratio have a practical application value in the treatment of nitrate-containing wastewater.These denitrifying bacteria could be added to CWs to enhance nitrogen removal efficiency of CWs for livestock wastewater with low C/N ratio in the future.
