The low-temperature plasma （LTP） generated by dielectric barrier discharge （DBD） was used to sterilize the E.coli endotoxin, which is usually difficult to kill by traditional methods. Three different concentration...The low-temperature plasma （LTP） generated by dielectric barrier discharge （DBD） was used to sterilize the E.coli endotoxin, which is usually difficult to kill by traditional methods. Three different concentrations of bacterial endotoxin （1 EU/mL, 0.5 EU/mL and 0.25 EU/mL） were treated by LTP for different time （20 s, 40 s and 60 s）. Tachypleus amebocyte lysate （TAL） method was employed to detect the concentration variation of bacterial endotoxin before and af- ter the plasma treatment, and endotoxic shock mice model was used to evaluate the inactivation effects of LTP on endotoxin for further study. Experimental results demonstrated that, DBD plasma can inactivate the bacterial endotoxin quickly and effectively, and when the LTP treatment time was increased, the concentrations of bacterial endotoxin decreased gradually （after 60 s plasma treatment, its inactivation effect was beyond the Chinese pharmacopoeia standard）, and the average survival time of mice gradually extended. The possible inactivation mechanisms are proposed to be related to reactive oxygen species （ROSs）.展开更多
基金supported in part by China Foundation for the Author of National Excellent Doctoral Dissertation(No.200338)Shaanxi Province Science and Technology Program(No.2010K16-04)+1 种基金the Fundamental Research Funds for the Central Universities (Nos.2009xjtujc18,xjj20100160)Guanghua Foundation for Medicine Innovation Research of China(No.0203419)
文摘The low-temperature plasma （LTP） generated by dielectric barrier discharge （DBD） was used to sterilize the E.coli endotoxin, which is usually difficult to kill by traditional methods. Three different concentrations of bacterial endotoxin （1 EU/mL, 0.5 EU/mL and 0.25 EU/mL） were treated by LTP for different time （20 s, 40 s and 60 s）. Tachypleus amebocyte lysate （TAL） method was employed to detect the concentration variation of bacterial endotoxin before and af- ter the plasma treatment, and endotoxic shock mice model was used to evaluate the inactivation effects of LTP on endotoxin for further study. Experimental results demonstrated that, DBD plasma can inactivate the bacterial endotoxin quickly and effectively, and when the LTP treatment time was increased, the concentrations of bacterial endotoxin decreased gradually （after 60 s plasma treatment, its inactivation effect was beyond the Chinese pharmacopoeia standard）, and the average survival time of mice gradually extended. The possible inactivation mechanisms are proposed to be related to reactive oxygen species （ROSs）.
文摘目的探讨细菌溶解产物与玉屏风颗粒对咳嗽变异性哮喘(cough variant asthma,CVA)患儿的疗效。方法选取108例CVA患儿作为研究对象,用随机数字表法分为西药组与联合组,每组54例。西药组接受CVA常规治疗,观察组采用细菌溶解产物联合玉屏风颗粒治疗,连续治疗2个月。比较2组的临床疗效、肺功能[用力肺活量(forced vital capacity,FVC)、第1秒用力呼气容积(forced expiratory volume in first second,FEV_(1))、呼气峰流量(peak expiratory flow,PEF)]、免疫功能[免疫球蛋白A(immunoglobulin A,IgA)、免疫球蛋白G(immunoglobulin G,IgG)和免疫球蛋白M(immunoglobulin M,IgM)]水平、儿童哮喘生存质量问卷(pediatric asthma quality of life questionnaire,PAQLQ)的变化以及不良反应。结果联合组的总有效率为92.60%,明显高于西药组的77.78%(P<0.05);FVC、FEV_(1)、PEF、IgA、IgG、IgM以及PAQLQ评分均明显高于西药组(P<0.05);2组患儿药物不良反应发生率比较差异无统计学意义(P>0.05)。结论细菌溶解产物联合玉屏风颗粒可有效提高CVA患儿的临床疗效,改善其肺部功能及免疫功能,提高患儿的生活质量。
