The poplar bacterial canker caused by Erwinia sp. is a serious disease spreading in most parts of Northeast China, where poplars are cultivated. The disease seriously infects those poplars belonging to section Aigeiro...The poplar bacterial canker caused by Erwinia sp. is a serious disease spreading in most parts of Northeast China, where poplars are cultivated. The disease seriously infects those poplars belonging to section Aigeiros or the intersection hybrids of section Tacamahaca and section Aigeiros and their clones. Five fine poplar cultivars resistant to the bacterial canker have been obtained by screening tests. They are Lz2(Populus simonii × nigra var. italica), A15(P. × sp.), 613(P. × sp.), A102and A98(P. × simonii × sp.). The following chemicals are obviously effective, such as: 50% DT germicide, 40% XF-136 and 10% C.C.M.A. or 12.5% ramphencol and 50,000 ppm/ml validamycin, with which the stems or the roots can be watered.展开更多
Objectives: Diagnostics is the first step for the treatment and eradication of infectious microbial diseases. Due to ever evolving pathogens and emerging new diseases, there is an urgent need to identify suitable diag...Objectives: Diagnostics is the first step for the treatment and eradication of infectious microbial diseases. Due to ever evolving pathogens and emerging new diseases, there is an urgent need to identify suitable diagnostic techniques for better management of each disease. The success rate of specific diagnostic technique in any population depends on various factors including type of the microbial pathogen, availability of resources, technical expertise, disease severity and degree of epidemic of disease in the area. One of the important tasks of the policy makers is to identify and implement suitable diagnostic techniques for specific regions based on their specific requirements. In this review we have discussed various techniques available in the literature and their suitability for the target population based on above mentioned criteria. Methods: Diagnostic techniques evaluation of well documented representative microbial diseases;Tuberculosis (bacterial), Malaria (parasitic) and HIV (viral) were included in the study. Identification and collection of information and data was performed focusing on the diagnostic techniques used from the scientific publications from Pubmed, Science Access, Scopus, EMBASE and several regional databases. WHO and CDC database for Tuberculosis, Malaria and HIV were also included. These techniques were compared with respect to the financial resource availability, expertise and management, functional capacity, pathogen virulence and degree of epidemic in the population. Results and Conclusion: In case of Tuberculosis, ELISA and colorimetric techniques are successful in rural and urban communities with 80% - 90% sensitivity. Genotyping and SNP analysis are useful in drug resistant strains. Parasitic disease Malaria also follows the same trend with diagnostic techniques like RDTs being common in both population with fast results and around 90% sensitivity. STD disease like HIV however shows slight different trends due to urgent need of interference in rural epidemics of the disease. Rapid and sensitive immunotechniques like dipsticks and agglutination with almost 100% sensitivity are used in both rural and urban areas. For the confirmation further tests are done like protein Western and NAAT. Advance techniques could be the option for higher epidemic area, drug resistance and disease research, while rapid techniques would be suitable for low income areas and POC facilities. Therefore, suitability of the diagnostic techniques for better management depends not only on the financial resources and assessment skills of a community but sometimes on the disease itself. We have further discussed the technological improvements for specific settings (rural/urban) based on the past research for better management of diseases, which could be implemented for the understanding of understudied and newly emerging diseases.展开更多
A two-year study on temporal variations in the ground water heterotrophic bacterial cell sizes of free living bacteria (FLB) and particle bound bacteria (PBB) from the agricultural, domestic and industrial areas was c...A two-year study on temporal variations in the ground water heterotrophic bacterial cell sizes of free living bacteria (FLB) and particle bound bacteria (PBB) from the agricultural, domestic and industrial areas was carried out from February 2005 to January 2007. The overall mean cell length of FLB and PBB was similar in all the ground water studied. However, the season wise grouped data revealed significant seasonal changes in cell length of FLB and PBB, as smaller bacteria were noticed during rainy season in the ground water in agricultural area in both the years, and only in the second year of study in domestic and industrial areas. Generally, it was noticed that there were summer maximum and rainy minimum values of the cell length of PBB in the ground water in agricultural, domestic and industrial areas in the second year of study. The Pearson's correlations showed the presence of 8 (in agricultural area), 5 (in domestic) and 3 (in industrial) significant correlations with environmental (Physico-chemical) parameters, respectively. The regression analysis revealed that as much as 12% of variation in the mean length of FLB was due to NO3 (+) in agricultural area and 9% due to total solids (+) in domestic area. However, the 8 % variation in bacterial cell size of FLB was due to Mg (+) in industrial area. Whereas, 13 % variation in mean length of PBB was due to SO4 (+) in agricultural area and 10% due to total anions of strong acid (TASA) (+) in domestic area. Furthermore, 10 % of variation was due to PO4 (+) in industrial area. Thus, the statistical analysis revealed that several environmental variables were potentially responsible for some of the temporal variations in aquatic heterotrophic bacterial cell size, suggesting probably the stressed environment in these ecosystems.展开更多
Expression of the nitrogen fixation (nif ) genes is tightly regulated by two proteins NifA and NifL in the (-subdivision of the proteobacteria. NifA is a transcriptional activator, which can be inactivated by NifL in ...Expression of the nitrogen fixation (nif ) genes is tightly regulated by two proteins NifA and NifL in the (-subdivision of the proteobacteria. NifA is a transcriptional activator, which can be inactivated by NifL in the presence of oxygen or excess fixed nitrogen. A direct interaction between E. cloacae NifL and NifA was detected using the bacterial two-hybrid system. This interaction was accelerated in the presence of fixed nitrogen, while oxygen had no effect. NifL proteins, with their C-terminus being deleted, completely lost the ability to interact with NifA. The data suggest that the C-terminal domain of NifL acts as a sensor of the nitrogen status of the cell and mediates interaction with NifA.展开更多
Background Bacterial vaginosis is a polymicrobial syndrome in which the homeostasis exerted by the Latobacillus species that protect the vaginal mucosa has been lost.This study explored the data balancing process with...Background Bacterial vaginosis is a polymicrobial syndrome in which the homeostasis exerted by the Latobacillus species that protect the vaginal mucosa has been lost.This study explored the data balancing process with the intention of improving the quality of association rules.The article aimed to balance the unbalanced multiclass dataset to improve association rule creation.Methods A dataset with 201 observations and 58 variables was analyzed.A preconstructed dataset was used.The authors collected the data between August 2016 and October 2018 in Tabasco,Mexico.The study population comprised sexually active women ages 18 to 50 who underwent gynecological inspection at the infectious and metabolic diseases research laboratory at the Universidad Juarez Autonoma de Tabasco.To determine the best κ-value,the random-forest algorithm was used and the balancing was performed with the synthetic minority over-sampling technique(SMOTE),random over-sampling examples(ROSE),and adaptive syntetic sampling approach for imbalanced learning(ADASYN)algorithms.The Apriori algorithm created the rules and to select rules with statistical significance,the is.redundant(),is.significant(),and is.maximal()functions and quality metric Fisher’s exact tes were used.The biological validation was carried out by the expert(bacteriologist).Results The ADASYN algorithm at K=9 the out of the bag(OOB)error was zero,this was the best𝐾-values.In the balancing process the ADASYN algorithm show best the performance.From the dataset balanced with ADASYN,the apriori algorithm created the association rules and the selection with the quality metric Fisher’s exact test,and the biological validation reported 13 rules.Gram-bacteria Atopobium vaginae,Gardnerella vaginalis,Megasphaera filotipo 1,Mycoplasma hominis and Ureaplasma parvum were detected by the apriori algorithm from the balanced dataset.Conclusion Balancing may improve the creation of association rules to efficiently model the bacteria that cause bacterial vaginosis.展开更多
Investigations into the intramolecular interactions of the native protein in solution are important to understand its structural stability as well as its potential uses in future applications.In this study,we used a b...Investigations into the intramolecular interactions of the native protein in solution are important to understand its structural stability as well as its potential uses in future applications.In this study,we used a bacterial two-hybrid system to investigate the interaction between the phycocyanin𝛼and𝛽subunits that form the phycocyanin monomer.Key amino acid residues responsible for the interaction between the subunits were identified,providing direct experimental evidence for the intramolecular interaction.展开更多
文摘The poplar bacterial canker caused by Erwinia sp. is a serious disease spreading in most parts of Northeast China, where poplars are cultivated. The disease seriously infects those poplars belonging to section Aigeiros or the intersection hybrids of section Tacamahaca and section Aigeiros and their clones. Five fine poplar cultivars resistant to the bacterial canker have been obtained by screening tests. They are Lz2(Populus simonii × nigra var. italica), A15(P. × sp.), 613(P. × sp.), A102and A98(P. × simonii × sp.). The following chemicals are obviously effective, such as: 50% DT germicide, 40% XF-136 and 10% C.C.M.A. or 12.5% ramphencol and 50,000 ppm/ml validamycin, with which the stems or the roots can be watered.
文摘Objectives: Diagnostics is the first step for the treatment and eradication of infectious microbial diseases. Due to ever evolving pathogens and emerging new diseases, there is an urgent need to identify suitable diagnostic techniques for better management of each disease. The success rate of specific diagnostic technique in any population depends on various factors including type of the microbial pathogen, availability of resources, technical expertise, disease severity and degree of epidemic of disease in the area. One of the important tasks of the policy makers is to identify and implement suitable diagnostic techniques for specific regions based on their specific requirements. In this review we have discussed various techniques available in the literature and their suitability for the target population based on above mentioned criteria. Methods: Diagnostic techniques evaluation of well documented representative microbial diseases;Tuberculosis (bacterial), Malaria (parasitic) and HIV (viral) were included in the study. Identification and collection of information and data was performed focusing on the diagnostic techniques used from the scientific publications from Pubmed, Science Access, Scopus, EMBASE and several regional databases. WHO and CDC database for Tuberculosis, Malaria and HIV were also included. These techniques were compared with respect to the financial resource availability, expertise and management, functional capacity, pathogen virulence and degree of epidemic in the population. Results and Conclusion: In case of Tuberculosis, ELISA and colorimetric techniques are successful in rural and urban communities with 80% - 90% sensitivity. Genotyping and SNP analysis are useful in drug resistant strains. Parasitic disease Malaria also follows the same trend with diagnostic techniques like RDTs being common in both population with fast results and around 90% sensitivity. STD disease like HIV however shows slight different trends due to urgent need of interference in rural epidemics of the disease. Rapid and sensitive immunotechniques like dipsticks and agglutination with almost 100% sensitivity are used in both rural and urban areas. For the confirmation further tests are done like protein Western and NAAT. Advance techniques could be the option for higher epidemic area, drug resistance and disease research, while rapid techniques would be suitable for low income areas and POC facilities. Therefore, suitability of the diagnostic techniques for better management depends not only on the financial resources and assessment skills of a community but sometimes on the disease itself. We have further discussed the technological improvements for specific settings (rural/urban) based on the past research for better management of diseases, which could be implemented for the understanding of understudied and newly emerging diseases.
文摘A two-year study on temporal variations in the ground water heterotrophic bacterial cell sizes of free living bacteria (FLB) and particle bound bacteria (PBB) from the agricultural, domestic and industrial areas was carried out from February 2005 to January 2007. The overall mean cell length of FLB and PBB was similar in all the ground water studied. However, the season wise grouped data revealed significant seasonal changes in cell length of FLB and PBB, as smaller bacteria were noticed during rainy season in the ground water in agricultural area in both the years, and only in the second year of study in domestic and industrial areas. Generally, it was noticed that there were summer maximum and rainy minimum values of the cell length of PBB in the ground water in agricultural, domestic and industrial areas in the second year of study. The Pearson's correlations showed the presence of 8 (in agricultural area), 5 (in domestic) and 3 (in industrial) significant correlations with environmental (Physico-chemical) parameters, respectively. The regression analysis revealed that as much as 12% of variation in the mean length of FLB was due to NO3 (+) in agricultural area and 9% due to total solids (+) in domestic area. However, the 8 % variation in bacterial cell size of FLB was due to Mg (+) in industrial area. Whereas, 13 % variation in mean length of PBB was due to SO4 (+) in agricultural area and 10% due to total anions of strong acid (TASA) (+) in domestic area. Furthermore, 10 % of variation was due to PO4 (+) in industrial area. Thus, the statistical analysis revealed that several environmental variables were potentially responsible for some of the temporal variations in aquatic heterotrophic bacterial cell size, suggesting probably the stressed environment in these ecosystems.
基金This work was supported by a grant in aid of the Shanghai Institutes of Biological Sciences, the Chinese Academy of Sciences "973" Project of the Ministry of Science and Technology, China (Grant No. 2001CB108901)
文摘Expression of the nitrogen fixation (nif ) genes is tightly regulated by two proteins NifA and NifL in the (-subdivision of the proteobacteria. NifA is a transcriptional activator, which can be inactivated by NifL in the presence of oxygen or excess fixed nitrogen. A direct interaction between E. cloacae NifL and NifA was detected using the bacterial two-hybrid system. This interaction was accelerated in the presence of fixed nitrogen, while oxygen had no effect. NifL proteins, with their C-terminus being deleted, completely lost the ability to interact with NifA. The data suggest that the C-terminal domain of NifL acts as a sensor of the nitrogen status of the cell and mediates interaction with NifA.
文摘Background Bacterial vaginosis is a polymicrobial syndrome in which the homeostasis exerted by the Latobacillus species that protect the vaginal mucosa has been lost.This study explored the data balancing process with the intention of improving the quality of association rules.The article aimed to balance the unbalanced multiclass dataset to improve association rule creation.Methods A dataset with 201 observations and 58 variables was analyzed.A preconstructed dataset was used.The authors collected the data between August 2016 and October 2018 in Tabasco,Mexico.The study population comprised sexually active women ages 18 to 50 who underwent gynecological inspection at the infectious and metabolic diseases research laboratory at the Universidad Juarez Autonoma de Tabasco.To determine the best κ-value,the random-forest algorithm was used and the balancing was performed with the synthetic minority over-sampling technique(SMOTE),random over-sampling examples(ROSE),and adaptive syntetic sampling approach for imbalanced learning(ADASYN)algorithms.The Apriori algorithm created the rules and to select rules with statistical significance,the is.redundant(),is.significant(),and is.maximal()functions and quality metric Fisher’s exact tes were used.The biological validation was carried out by the expert(bacteriologist).Results The ADASYN algorithm at K=9 the out of the bag(OOB)error was zero,this was the best𝐾-values.In the balancing process the ADASYN algorithm show best the performance.From the dataset balanced with ADASYN,the apriori algorithm created the association rules and the selection with the quality metric Fisher’s exact test,and the biological validation reported 13 rules.Gram-bacteria Atopobium vaginae,Gardnerella vaginalis,Megasphaera filotipo 1,Mycoplasma hominis and Ureaplasma parvum were detected by the apriori algorithm from the balanced dataset.Conclusion Balancing may improve the creation of association rules to efficiently model the bacteria that cause bacterial vaginosis.
基金supported by the National Science Foundation of China(31900023,U2006205)the State Key Laboratory of Microbial Technology Open Projects Fund(M2021-08).
文摘Investigations into the intramolecular interactions of the native protein in solution are important to understand its structural stability as well as its potential uses in future applications.In this study,we used a bacterial two-hybrid system to investigate the interaction between the phycocyanin𝛼and𝛽subunits that form the phycocyanin monomer.Key amino acid residues responsible for the interaction between the subunits were identified,providing direct experimental evidence for the intramolecular interaction.
