Pathogenic Escherichia coli strains were isolated from yellow and white scour samples in pig farms. The strains were used to make autologous inactivated bacterins,and the bacterins were applied to multiple infected fa...Pathogenic Escherichia coli strains were isolated from yellow and white scour samples in pig farms. The strains were used to make autologous inactivated bacterins,and the bacterins were applied to multiple infected farms. The results showed that in the group of inactivated bacterins,the infected piglet number was less,the course of disease was shorter and the symptoms was milder. The piglet E. coli inactivated bacterins could significantly reduced morbidity of yellow and white scour of piglets,the preventive effects of E. coli autologous inactivated bacterins against yellow and white scour of piglets were much better than that of genetically engineered vaccines.展开更多
The aim of the study was to investigate the immune effect of fusion protein VP4-STI. 40 mouse were randomly divided into 4 groups of test bacterin group （30 μg VP4-STI ＋0.6 μg LTB）, aluminiumhydroxide vaccine gro...The aim of the study was to investigate the immune effect of fusion protein VP4-STI. 40 mouse were randomly divided into 4 groups of test bacterin group （30 μg VP4-STI ＋0.6 μg LTB）, aluminiumhydroxide vaccine group （30 μg VP4-STI ＋Al（OH）3 gel ） , pure protein VP4- STI vaccine group （30μg VP4-STI ） and PBS control group were immunized by rhinal dripping. And then, the antibody levels of the mouse were determined. The protection effects of mouse in all immune groups were observed after the toxicity test with strong virulent strain C83902 of E. coli. Anti-VP4-STI antibodies were produced in other groups with the highest at the 6th week, except PBS control group. The antibody level in aluminiumhydroxide bactetin group was higher than that in test vaccine group. The antibody level in pure protein VP4-STI bacterin group was lower, being extremely significantly different from that in test vaccine group （ P 〈0. 001 ）. Mouse in test vaccine group and aluminium hydroxide bacterin group had better immuno-protection effect on strong virulent strain C83902 of E. coli, obviously different from that in PBS control group. The research provided basis for further improving the immunogeneticity of STI.展开更多
基金Supported by Public Welfare(agriculture)Industry-specific of the Ministry of Agriculture(201303044)Hunan Science Program(2011NK3162)Hunan Provincial Department of Education Science Research Project(12C1204)
文摘Pathogenic Escherichia coli strains were isolated from yellow and white scour samples in pig farms. The strains were used to make autologous inactivated bacterins,and the bacterins were applied to multiple infected farms. The results showed that in the group of inactivated bacterins,the infected piglet number was less,the course of disease was shorter and the symptoms was milder. The piglet E. coli inactivated bacterins could significantly reduced morbidity of yellow and white scour of piglets,the preventive effects of E. coli autologous inactivated bacterins against yellow and white scour of piglets were much better than that of genetically engineered vaccines.
基金Supported by the Doctor Foundation of Xinjiang Production and Con-struction Corps(Bingbo02,NKBOISHZO8)~~
文摘The aim of the study was to investigate the immune effect of fusion protein VP4-STI. 40 mouse were randomly divided into 4 groups of test bacterin group （30 μg VP4-STI ＋0.6 μg LTB）, aluminiumhydroxide vaccine group （30 μg VP4-STI ＋Al（OH）3 gel ） , pure protein VP4- STI vaccine group （30μg VP4-STI ） and PBS control group were immunized by rhinal dripping. And then, the antibody levels of the mouse were determined. The protection effects of mouse in all immune groups were observed after the toxicity test with strong virulent strain C83902 of E. coli. Anti-VP4-STI antibodies were produced in other groups with the highest at the 6th week, except PBS control group. The antibody level in aluminiumhydroxide bactetin group was higher than that in test vaccine group. The antibody level in pure protein VP4-STI bacterin group was lower, being extremely significantly different from that in test vaccine group （ P 〈0. 001 ）. Mouse in test vaccine group and aluminium hydroxide bacterin group had better immuno-protection effect on strong virulent strain C83902 of E. coli, obviously different from that in PBS control group. The research provided basis for further improving the immunogeneticity of STI.
