Using a special property of dynamic complementary-suppression-modulated transmission （DCSMT） in the bacteriorhodopsin （bR） film, we have demonstrated an all-optical time-delay relay. To extend our work, the relati...Using a special property of dynamic complementary-suppression-modulated transmission （DCSMT） in the bacteriorhodopsin （bR） film, we have demonstrated an all-optical time-delay relay. To extend our work, the relationship between the delay time of the all-optical time-delay relay and parameters of a bR film is numerically studied. We show how the delay time changes with the product of concentration and thickness （PCT） of a bR film. Furthermore, the shortest and longest delay times are given for the relay of ＇switch off. The saturable delay time and maximum delaytime of ＇switch on＇ are also given. How the wavelengths （632.8, 568, 533 and 412 nm） and intensities of the illuminating light influence the delay time is also discussed. The simulation results are useful for optimizing the design of all-optical time-delay relays.展开更多
The quality of the novelty filter image is investigated at different intensities of the incident blue and yellow beams irradiating a bacteriorhodopsin （bR） film. The relationship between the transmitted blue beams a...The quality of the novelty filter image is investigated at different intensities of the incident blue and yellow beams irradiating a bacteriorhodopsin （bR） film. The relationship between the transmitted blue beams and the incident yellow beams is established. The results show that the contrast of the novelty filter image depends on the lifetime of longest lived photochemical state （M state）. These results enable one to identify the direction of a moving object and to improve the quality of the novel filter image by prolonging the lifetime of M state.展开更多
Bacteriorhodopsin(BR)exhibits,as a membrane protein in Halobacterium salinarum,unique photoresponsive behaviors,and shows promise as a functional information material.A new mutant of BR with the 96th aspartic acid rep...Bacteriorhodopsin(BR)exhibits,as a membrane protein in Halobacterium salinarum,unique photoresponsive behaviors,and shows promise as a functional information material.A new mutant of BR with the 96th aspartic acid replaced by valine(BR-D96V)was obtained and then a composite film of BR-D96V in a synthetic polymer matrix was prepared in this research.The mutant BR-D96V was expressed in a bacterio-opsin deficient halobacterial strain(L33)by gene engineering.Although valine is very hydrophobic,this point mutant keeps the basic biological activities,namely,photoelectric and photochromic responses.Nevertheless,the lifetime of M intermediate in the BR mutant is nearly two orders of magnitude longer than that of wild-type BR in neutral aqueous solution,which benefits its potential application as an information material.The M lifetime is further significantly prolonged after embedding BR-D96V into poly(vinyl alcohol)(PVA).It was also found that BR-D96V is very sensitive to water content in comparison with wild-type BR and another BR mutant.展开更多
With the rise of studying biomolecular electronics and biocomputer since the 1980s, bactefiorhodopsin (BR) of purple membrane (PM) from Halobacteria halobium has become an outstanding candidate for studying biomolecul...With the rise of studying biomolecular electronics and biocomputer since the 1980s, bactefiorhodopsin (BR) of purple membrane (PM) from Halobacteria halobium has become an outstanding candidate for studying biomolecular devices due to its stable structure, light-driven proton pumping capability, bistable property, photoelectric responses from ps to ms, as well as its potential application to developing biomolecular devices, biochips and so on. However, the BR not only must, but also can be oriented due展开更多
Bacteriorhodopsin is the only protein in purple membrane. When illuminated, optically distinct intermediates K, L, M and O are known to appear, then it regenerates itself at the end of the photocycle. In the photocycl...Bacteriorhodopsin is the only protein in purple membrane. When illuminated, optically distinct intermediates K, L, M and O are known to appear, then it regenerates itself at the end of the photocycle. In the photocycle, the proton is released. So the function of purple membrane is a light-driven proton pump. The unique structure and function of purple membrane are an ideal material for investigating energy conversion. In recent years,展开更多
Ten layers of oriented bacteriorhodopsin (BR) LB films are prepared on ITO conductive glass toconstitute an ITO electrode/BR LB films/KCl solution/Cu electrode wet photocell.A Q-switched double frequency Nd:YAG laser ...Ten layers of oriented bacteriorhodopsin (BR) LB films are prepared on ITO conductive glass toconstitute an ITO electrode/BR LB films/KCl solution/Cu electrode wet photocell.A Q-switched double frequency Nd:YAG laser with 10 Hz repetitive rate and 8 ns pulse width is used to excite the BR LB films.The photoelectric signal is directly captured by a 300 MHz digitizing oscilloscope.A theory on the BR photoelectric dynamics is established.By nonlinear least square fitting of the experimental curves acquired at different time bases,the experimental equation corresponding to the theoretical model is obtained,and the charge displacements and the decay time constants for each step in the BR photocycle are calculated.展开更多
Langmuir-Blodgett films containing bacteriorhodopsin were deposited on ITO conduc-tive electrodes.A sandwiched photocell with a junction structure of ITO/bR/electrolyte/ITO has beenconstructed,in which the bR LB film ...Langmuir-Blodgett films containing bacteriorhodopsin were deposited on ITO conduc-tive electrodes.A sandwiched photocell with a junction structure of ITO/bR/electrolyte/ITO has beenconstructed,in which the bR LB film was directly put into contact with an aqueous electrolyte immobi-lized in an agar gel.Under visible light irradiation,the photocell produced a transient photocurrent due tothe change of light intensity,which characterized vision imitative material.A photoalarm device based ondifferential responsivity phenomena was fabricated.展开更多
基金Project supported by the National Natural Science Foundation of China (Grant Nos 10774152, and 60578020)the Natural Science Foundation of Shangdong Province, China (Grant No Y2006A01)the Key Laboratory of Weak Light Nonlinear Photonics
文摘Using a special property of dynamic complementary-suppression-modulated transmission （DCSMT） in the bacteriorhodopsin （bR） film, we have demonstrated an all-optical time-delay relay. To extend our work, the relationship between the delay time of the all-optical time-delay relay and parameters of a bR film is numerically studied. We show how the delay time changes with the product of concentration and thickness （PCT） of a bR film. Furthermore, the shortest and longest delay times are given for the relay of ＇switch off. The saturable delay time and maximum delaytime of ＇switch on＇ are also given. How the wavelengths （632.8, 568, 533 and 412 nm） and intensities of the illuminating light influence the delay time is also discussed. The simulation results are useful for optimizing the design of all-optical time-delay relays.
基金Project supported by the National Natural Science Foundation of China （Grant No 60578020）, and Science ＆ Technology Foundation of Tianjin University, China （Grant No 20050419）.
文摘The quality of the novelty filter image is investigated at different intensities of the incident blue and yellow beams irradiating a bacteriorhodopsin （bR） film. The relationship between the transmitted blue beams and the incident yellow beams is established. The results show that the contrast of the novelty filter image depends on the lifetime of longest lived photochemical state （M state）. These results enable one to identify the direction of a moving object and to improve the quality of the novel filter image by prolonging the lifetime of M state.
基金supported by the National Basic Research Program of China(2009CB930000)National Natural Science Foundation of China(50533010)
文摘Bacteriorhodopsin(BR)exhibits,as a membrane protein in Halobacterium salinarum,unique photoresponsive behaviors,and shows promise as a functional information material.A new mutant of BR with the 96th aspartic acid replaced by valine(BR-D96V)was obtained and then a composite film of BR-D96V in a synthetic polymer matrix was prepared in this research.The mutant BR-D96V was expressed in a bacterio-opsin deficient halobacterial strain(L33)by gene engineering.Although valine is very hydrophobic,this point mutant keeps the basic biological activities,namely,photoelectric and photochromic responses.Nevertheless,the lifetime of M intermediate in the BR mutant is nearly two orders of magnitude longer than that of wild-type BR in neutral aqueous solution,which benefits its potential application as an information material.The M lifetime is further significantly prolonged after embedding BR-D96V into poly(vinyl alcohol)(PVA).It was also found that BR-D96V is very sensitive to water content in comparison with wild-type BR and another BR mutant.
基金Project supported by the National Natural Science Foundation of China
文摘With the rise of studying biomolecular electronics and biocomputer since the 1980s, bactefiorhodopsin (BR) of purple membrane (PM) from Halobacteria halobium has become an outstanding candidate for studying biomolecular devices due to its stable structure, light-driven proton pumping capability, bistable property, photoelectric responses from ps to ms, as well as its potential application to developing biomolecular devices, biochips and so on. However, the BR not only must, but also can be oriented due
基金Project supported by the National Natural Science Foundation of China
文摘Bacteriorhodopsin is the only protein in purple membrane. When illuminated, optically distinct intermediates K, L, M and O are known to appear, then it regenerates itself at the end of the photocycle. In the photocycle, the proton is released. So the function of purple membrane is a light-driven proton pump. The unique structure and function of purple membrane are an ideal material for investigating energy conversion. In recent years,
基金Project supported by the State Key Laboratory of Transient OpticsTechnology and Bureau of Foundamental Researches, Chinese Academy of Sciences.
文摘Ten layers of oriented bacteriorhodopsin (BR) LB films are prepared on ITO conductive glass toconstitute an ITO electrode/BR LB films/KCl solution/Cu electrode wet photocell.A Q-switched double frequency Nd:YAG laser with 10 Hz repetitive rate and 8 ns pulse width is used to excite the BR LB films.The photoelectric signal is directly captured by a 300 MHz digitizing oscilloscope.A theory on the BR photoelectric dynamics is established.By nonlinear least square fitting of the experimental curves acquired at different time bases,the experimental equation corresponding to the theoretical model is obtained,and the charge displacements and the decay time constants for each step in the BR photocycle are calculated.
基金the National Natural Science Foundation of China
文摘Langmuir-Blodgett films containing bacteriorhodopsin were deposited on ITO conduc-tive electrodes.A sandwiched photocell with a junction structure of ITO/bR/electrolyte/ITO has beenconstructed,in which the bR LB film was directly put into contact with an aqueous electrolyte immobi-lized in an agar gel.Under visible light irradiation,the photocell produced a transient photocurrent due tothe change of light intensity,which characterized vision imitative material.A photoalarm device based ondifferential responsivity phenomena was fabricated.
