EPES has proved to stabilize M intermediate of Bacteriorhodopsin. Absorp-tion and Raman spectra showed that there was a more stable form of M intermedi-ate in BR-HEPES solution. It was found by SPS that there were two...EPES has proved to stabilize M intermediate of Bacteriorhodopsin. Absorp-tion and Raman spectra showed that there was a more stable form of M intermedi-ate in BR-HEPES solution. It was found by SPS that there were two main interme-diates . M and O , existing in BR-HEPES solution.展开更多
The steady absorption and kinetic changes of M 412 intermediate of the light and dark adapted bacteriorhodopsin (BR) solubilized by different concentrated Triton X 100 were investigated. The results indicated that the...The steady absorption and kinetic changes of M 412 intermediate of the light and dark adapted bacteriorhodopsin (BR) solubilized by different concentrated Triton X 100 were investigated. The results indicated that the cooperative effect existing within the trimeric BR of native purple membrane (PM) was damaged in the system containing the surfactant since the component and structure of the bilayer lipid membrane in PM varied due to the solubilization of partial PM lipids by Triton X 100. The destruction to the cooperative effect of BR ultimately caused 13 cis retinal of the dark adapted BR to take part in BR photocycle and also to generate the deprotonated M 412 intermediate.展开更多
Bacteriorhodopsin （bR） in purple membrane （PM） from H. Halobium is a light-drivenproton pump. Its seven transmembrane helices form an internal proton channel. Anall-trans retinal covalently links up via protonated...Bacteriorhodopsin （bR） in purple membrane （PM） from H. Halobium is a light-drivenproton pump. Its seven transmembrane helices form an internal proton channel. Anall-trans retinal covalently links up via protonated Schiff base with K216 inside thechannel. Upon illumination, the retinal isomerization around the C<sub>13</sub>-C<sub>14</sub> double bond drivesprotons translocation through the channel from the cytoplasm into the medium, and展开更多
文摘EPES has proved to stabilize M intermediate of Bacteriorhodopsin. Absorp-tion and Raman spectra showed that there was a more stable form of M intermedi-ate in BR-HEPES solution. It was found by SPS that there were two main interme-diates . M and O , existing in BR-HEPES solution.
文摘The steady absorption and kinetic changes of M 412 intermediate of the light and dark adapted bacteriorhodopsin (BR) solubilized by different concentrated Triton X 100 were investigated. The results indicated that the cooperative effect existing within the trimeric BR of native purple membrane (PM) was damaged in the system containing the surfactant since the component and structure of the bilayer lipid membrane in PM varied due to the solubilization of partial PM lipids by Triton X 100. The destruction to the cooperative effect of BR ultimately caused 13 cis retinal of the dark adapted BR to take part in BR photocycle and also to generate the deprotonated M 412 intermediate.
基金Project supported by the Key Foundation of Chinese Academy of Sciences.
文摘Bacteriorhodopsin （bR） in purple membrane （PM） from H. Halobium is a light-drivenproton pump. Its seven transmembrane helices form an internal proton channel. Anall-trans retinal covalently links up via protonated Schiff base with K216 inside thechannel. Upon illumination, the retinal isomerization around the C<sub>13</sub>-C<sub>14</sub> double bond drivesprotons translocation through the channel from the cytoplasm into the medium, and
