The proprotein convertase subtilisin/kexin type 9 geneE670G polymorphism and serum lipid levels in the Guangxi Bai Ku Yao and Han populations

Background The association of E670G polymorphism in the proprotein convertase subtilisin/kexin type 9 (PCSK9) gene and serum lipid profiles is inconsistent in dif- ferent ethnic groups.Bai Ku Yao is a special subgroup of the Yao minority in China.The present study was undertaken association of PCSK9 E670G polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations.Methods A total of 649 subjects of Bai Ku Yao and 646 participants of Han Chinese were randomly selected from our previous stratified randomized cluster samples.Genotyping of the PCSK9 E670G polymorphism was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis,and then confirmed by direct sequencing. Results The levels of serum total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C) and apolipoprotein(Apo) AI were lower in Bai Ku Yao than in Han(P【0.01 for all).The frequency of A and G alleles was 98.00%and 2.00%in Bai Ku Yao,and 95.20%and 4.80%in Han(P【0.01);respectively. The frequency of AA,AG and GG genotypes was 95.99%,4.01%and 0%in Bai Ku Yao,and 91.02%, 8.36%and 0.62%in Han(P【0.01);respectively.There were also significant differences in the genotypic and allelic frequencies between n and the ratio of ApoAI to ApoB in Han Chinese but not in Bai Ku Yao were different between the AA and AG/GG genotypes(P【0.05 for all).The G allele carriers had higher serum HDL-C and higher ApoAI to ApoB ratio than the G allele noncarriers.When serum lipid parameters in Han were analyzed according to sex,the G allele carriers had higher serum HDL and ApoAI levels in males (P【0.05),and lower ApoB level and higher ApoAI to ApoB ratio in females(P【0.05 for all).Multiple linear regression analysis showed that serum HDL-C levels were correlated with genotypes in both ethnic groups(P【0.05 each).Serum lipid parameters were also correlated with sex,age,body massindex,alcohol consumption,cigarette smoking,and blood pressure in both ethnic groups(P【0.05-0.001).Conclusions These results suggest that the PCSK9 E670G polymorphism is mainly associated with some serum lipid parameters in the Han population,both gender show different relations to different serum lipid parameters.The G allele carriers might have higher serum lipid profiles than the G allele noncarriers. ormal LDL-C(≤3.20 mmol/L) and high LDL-C subgroups (】 3.20 mmol/L,P【0.01;respectively) in Bai Ku Yao, and between normal ApoB(≤1.14 g/L) and high ApoB subgroups(】 1.14 g/L,P 【 0.01;respectively) in Han.

Association of methylenetetrahydrofolate reductase C677T polymorphism and serum lipid levels in the Guangxi Bai Ku Yao and Han populations

Objectives The association of methylenetetrahy-drofolate reductase(MTHFR) gene polymorphism and serum lipid profiles is still controversial in diverse ethnics.Bai Ku Yao is an isolated subgroup of the Yao minority in China. The aim of the present study was to eveluate the association of MTHFR C677Tpolymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations.Methods A total of 780 subjects of Bai Ku Yao and 686 participants of Han Chinese were randomly selected from our previous stratified randomized cluster samples.Genotyping of the MTHFR C677T was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis,and then confirmed by direct sequencing.Results The levels of serum total cholesterol(TC),high-density lipoprotein cholesterol (HDL-C),low-density lipoprotein cholesterol(LDL-C), apolipoprotein(Apo) AI and ApoB were lower in Bai Ku Yao than in Han(P【0.05-0.001).The frequency of C and T alleles was 77.4%and 22.6%in Bai Ku Yao,and 60.9%and 39.1%in Han(P【0.001);respectively.The frequency of CC,CT and TT genotypes was 58.7%,37.3%and 4.0%in Bai Ku Yao,and 32.6%,56.4%and 11.0%in Han(P【 0.001);respectively.The levels of TC and LDL-C in both ethnic groups were significant differences among the three genotypes(P【0.05-0.01).The T allele carriers had higher serum TC and LDL-C levels than the T allele noncarriers. The levels of ApoB in Han were significant differences among the three genotypes(P【0.05).The T allele carriers had higher serum ApoB levels as compared with the T allele noncarriers. The levels of TC,TG and LDL-C in Bai Ku Yao were correlated with genotypes(P【0.05-0.001),whereas the levels of LDL-C in Han were associated with genotypes(P【 0.001).Serum lipid parameters were also correlated with sex, age,body massindex,alcohol consumption,cigarette smoking, and blood pressure in the both ethnic groups.Conclusions The differences in serum TC,TG,LDL-C and ApoB levels between the two ethnic groups might partly result from different genotypic and allelic frequencies of the MTHFR C677Tor differentMTHFR gene-enviromental interactions.

Relationship between the low density lipoprotein receptor gene polymorphism and serum lipid levels in the Guangxi Bai Ku Yao population

Objectives Bai Ku Yao(White-trousers Yaos)is a special branch of Yao minority in China.They are now living in both Lihu and Baxu villages,Nandan County, Guangxi,China.The population size is about 30,000.The special customs and culture of Bai Ku Yao,including their special clothing,intra-ethnic marriages and alcohol intake are still completely conserved to the present day.In previous epidemiologic studies,we found that the serum lipid levels and the prevalence of hyperlipidaemia were lower in Bai Ku Yao than in Han Chinese from the same region.This ethnic difference in serum lipid profiles is still not well known.We hypothesized that there may be significant differences in some genetic polymorphismsssociation of low density lipoprotein receptor (LDL-R) genepolymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations.Methods A total of 1024 subjects of Bai Ku Yao and 792 participants of Han Chinese were stud- ied by a stratified randomized cluster sampling.Epidemiological survey was carried out using internationally standardized methods.Information on demographics,socioeconomic status, and lifestyle factors was collected with standardized questionnaires. The height,weight,waist circumference,blood pressure, and serum total cholesterol(TC),triglyceride(TG), high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C),apolipoprotein(Apo) A1, and ApoB were measured.Body massindex(BMI,kg/m2) was calculated.Genotyping of the LDL-RAvaⅡwas performed by polymerse chain reaction and restriction fragment length polymorphism combined with gel electrophoresis,and then confirmed by direct sequencing.Results(l)The height,weight,serum TC,HDL-C,LDL-C,ApoAl levels and the ratio of ApoAl to ApoB were lower in Bai Ku Yao than in Han Chinese(P【0.01 for all),whereas the percentage of subjects who consumed alcohol or smoked cigarettes was higher in Bai Ku Yao than in Han Chinese(P【0.01 for each).(2) The frequency of A+ allele in Bai Ku Yao was 34.5%,and the frequencies of A-A-,A-A+ and A+A + genotypes were 42.6%,45.9%and 11.5%;respectively. The frequency of A+ allele in Han Chinese was 19.3%(P【0.001),and the frequencies of A-A-,A-A + and A+A+ genotypes were 64.9%,31.6%and 3.5%(P【0.001);respectively. The frequencies of A-A-,A-A+ and A+A+ genotypes in Bai Ku Yao were significant difference between males and females,between normal TC and high TC subgroup, and between normal LDL-C and high LDL-C subgroup (P【0.05 for all),whereas the frequencies of A- and A+ ? alleles in Han Chinese were significant difference between males and females(P【0.05).(3) Serum LDL-C levels in Bai Ku Yao were significant difference among the A-A-, A-A+ and A+A+ genotypes(P【0.05),the A+ carriers had higher serum LDL-C levels.Serum HDL-C levels in Han Chiese were significant difference among the A-A-,A-A + and A+A+ genotypes(P【0.01),the A+ carriers had higher serum HDL-C levels.(4) After adjusting other factors,the prevalence of LDL-C abnormality was still higher in Han Chiese than in Bai Ku Yao.The prevalence of TC abnormality in Han Chinese was almost twice high as in Bai Ku Yao. The age and diet were common risk factor for TC abnormality. No effect of AvaⅡgenotype or alcohol consumption on the TC abnormality was found,but the combination of geno-type and alcohol consumption can increase the prevalence of TC abnormality[Exp(B) =(1.154)].Age was negatively cor- related with TG level.Conclusions Serum TC and LDL-C levels were lower in Bai Ku Yao than in Han Chinese.There were significant differences in the AvaⅡallele and genotype frequencies between the he A+ carriers in Bai Ku Yao had higher serum LDL-C levels,whereas the A+ carriers in Han had higher serum HDL-C levels.Interactions between alcohol consumption or cigarette smoking and the LDL-R AvaⅡgenotype were also observed.The differences in the serum lipid profiles between the two ethnic groups might partly result from different genotypic frequency of LDL-R AvaⅡpolymorphism or differentgene-enviromental interactions.Bai Ku Yao and Han population,the frequency of A + allele was higher in Bai Ku Yao than in Han.T between the two ethnic groups.Therefore,the aim of the present study was to detect the

Peroxisome proliferator-activated receptor delta+294T>C polymorphism and serum lipid levels in the Guangxi Bai Ku Yao and Han populations

Objectives The association of peroxisome prolif-erator -activated receptor delta(PPARD) +294T】C polymorphism and serum lipid levels is inconsistent in several previous studies.Bai Ku Yao is an isolated association of PPARD +294T】C(rs2016520) polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations.Methods A total of 609 subjects of Bai Ku Yao and 573 participants of Han Chinese were randomly selected from our previous stratified randomized cluster samples.Genotyping of the PPARD +294T】C polymorphism was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis, and then confirmed by direct sequencing.Results The levels of serum total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),apolipoprotein(Apo) AI and ApoB were lower in Bai Ku Yao than in Han(P【0.001 for all).The frequency of T and C alleles was 77.50%and 22.50%in Bai Ku Yao,and 72.43%and 27.57%in Han (P【0.01);respectively.The frequency of TT,TC and CC genotypes was 60.59%,33.83%and 5.53%in Bai Ku Yao, and 52.18%,40.50%and 7.32%in Han(P【0.05);respectively. The levels of LDL-C,ApoB and the ratio of ApoAI to ApoB in Bai Ku Yao were different among the three genotypes in females but not in males(P【0.05 for all).The subjects with TT and TC genotypes had lower serum LDL-C and ApoB levels and higher the ratio of ApoAI to ApoB than the CC genotype in females.The levels of TC and ApoB in the total Han population were different among the three genotypes (P【0.05 for all).The C allele carriers had higher serum TC and ApoB levels than the C allele noncarriers.When serum lipid levels were analyzed according to sex,the difference in serum TC levels in Han was significant in males(P【0.01) but not in females,whereas the difference in serum ApoB levels was significant in females(P【0.05)but not in males. Serum TC and ApoB levels were correlated with genotypes in Han(P【0.05 for each) but not in Bai Ku Yao.Serum lipid parameters were also correlated with sex,age,body massindex, alcohol consumption,cigarette smoking,and blood pressure in both ethnic groups.Conclusions These results suggest that the association of PPARD +294T】C polymorphism and serum lipid levels is different between the Bai Ku Yao and Han populations.The discrepancy between the two ethnic groups might partly result from different PPARD +294T】C polymorphism or PPARD gene-enviromental interactions,subgroup of the Yao minority in China.

Association of the LIPG 584C>T polymorphism and serum lipid levels in the Guangxi Bai Ku Yao and Han populations

Objectives Endotheliallipase(EL) is a major determinantof high-density lipoprotein-cholesterol(HDL-C) metabolism,but the association of endothelial lipase gene (LIPG) polymorphism and serum HDL-C levels is scarce and conflicting in diverse populations.Bai Ku Yao is an isolated subgroup of the Yao minority in China.This study was designed to detect the association of LIPG 584C】T(rs2000813) polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations. Methods A total of 645 subjects of Bai Ku Yao and 638 participants of Han Chinese were randomly selected from our previous stratified randomized cluster samples.Genotyping of the LIPG584C】T was performed by polymerse chain reaction and restriction fragment length polymorphism combined with gel electrophoresis,and then confirmed by direct sequencing. Results The levels of serum total cholesterol(TC),HDL-C, low-density lipoprotein cholesterol(LDL-C) and apolipoprotein (Apo) AI and ApoB were lower in Bai Ku Yao than in Han(P【0.05-0.001).The frequency of C and T alleles was 73.5%and 26.5%in Bai Ku Yao,and 67.9%and 32.1%in Han(P【0.01).The frequency of CC,CT and TT genotypes was 50.4%,46.2%and 3.4%in Bai Ku Yao,and 41.4%, 53.1%and 5.5%in Han(P【0.01);respectively.Serum HDL-C levels in both ethnic groups were different among the three genotypes(P【0.05 for each).Serum TC levels in both ethnic groups were also different between CC and CT/TT types(P【 0.05 for each).The T allele carriers had higher serum HDL-C and TC levels than the T allele noncarriers.Multivariate logistic regression analysis showed that the levels of HDL-C and ApoB were correlated with genotype in Bai Ku Yao(P【 0.05 for each),whereas the levels of TC and HDL-C were associated with genotype in Han Chinese(P【0.05 and P【 0.01).Serum lipid parameters were also correlated with gender, age,weight,body massindex,alcohol consumption, cigarette smoking,and blood pressure in the both ethnic groups.Conclusions The frequency of LIPG 584Tallele is lower in the Bai Ku Yao than in the Han populations.The LIPG 584Tallele is associated with serum HDL-C and TC levels.The differences in serum lipid profiles between the two ethnic groups might partly result from different genotypic and allelic of LIPG 584C 】T or different LIPG-enviromental interactions.

南丹白裤瑶服饰文化意蕴及制作工艺特征

服饰文化不仅凝结了白裤瑶人特殊的民族情感、民族信仰,也反映了白裤瑶人的价值观、生存观,服饰文化研究是研究白裤瑶人传统文化的重要途径。文章运用文献资料法、田野调查法、逻辑分析法,对南丹白裤瑶族服饰文化意蕴及制作工艺特征展开深入研究,研究认为,白裤瑶服饰文化是白裤瑶人的精神纽带,它不仅强化了族群的团结和认同,也使每个族人在其中找到了归属感和文化自信。

南丹白裤瑶民族服饰的审美取

白裤瑶服饰有着鲜明的地域特征和浓郁的生活气息,展现了白裤瑶人独特的审美情趣。为了进一步了解白裤瑶人的服饰审美取向,通过文献分析和实物考察,文章着重从造型、色彩、纹样等方面介绍了白裤瑶服饰,并探索南丹白裤瑶民族服饰美学的价值,希望借此推动白裤瑶传统服饰文化的传承和发展。

非遗视角下白裤瑶黏膏树脂染工艺的调查研究

黏膏树脂染是白裤瑶最具特色的传统工艺,笔者两次深入广西壮族自治区河池市南丹县实地调查白裤瑶黏膏树脂染技艺,对其工艺流程及工艺过程中使用到的各种材料和工具进行了详尽阐述,并对白裤瑶民族服饰传承人和瑶族原生态蓝靛蜡染坊坊主进行了访谈调查,从非遗保护和传承的视角讨论白裤瑶黏膏树脂染的现状、发展和变化。

白裤瑶铜鼓舞价值研究

采用文献资料法、访谈法和调查法,对白裤瑶铜鼓舞的起源、铜鼓舞与白裤瑶的砍牛祭习俗等进行研究,着重探讨了白裤瑶铜鼓舞的社会价值。结果表明:铜鼓舞具有传承民族文化、增强民族凝聚力、健身娱乐、表演、保护与自卫以及促进地方经济发展等价值。

桂黔白裤瑶母性崇拜的文化人类学考察

在人类历史漫长的发展过程中,母性崇拜是一种普遍存在的民俗现象,母性崇拜意识一直是人类社会挥之不去共通性情节。广西与贵州边界的白裤瑶族群的母性崇拜意识有其自身独特的文化表征形态,其主要表现在供奉生殖图腾石的习俗"、姑女还舅门"婚俗、婚前盛行"玩婊"、妇女夏天身着"挂衣"、服饰图案的"卍"符号等方面。

白裤瑶服饰文化的意义与象征

白裤瑶是瑶族的一个支系,人口仅2万余人,聚居在黔桂边境的广西南丹县里湖一带。其服饰工艺精湛,样式简朴古老,是白裤瑶民族历史和文化研究极重要的证据。本文从白裤瑶的宗教信仰和崇拜、自然环境的影响和原始文化特征等方面研究白裤瑶服饰的人类学意义,论述白裤瑶服饰所阐释的古老的民族文明形式。

斑斓的记忆——白裤瑶服饰研究

广西壮族自治区南丹县的"瑶族服饰"被列入我国"第一批国家级非物质文化保护名录",与别的瑶族服饰不同,南丹县的"瑶族服饰"自有特点,有其独特的文化内涵。本文根据长期的调查研究,对白裤瑶人的服装进行解读,以证明白裤瑶人在服饰上表现的图腾是白腹锦鸡。

冲突与融合:南丹白裤瑶婚姻习惯法与国家法之互动(上)——广西世居民族习惯法研究系列论文之三

白裤瑶婚姻习惯法作为一项地方性知识,已经被民众普遍接受并成为其生活准则的重要组成部分。本文从婚约、结婚、离婚等方面描述了传统婚姻习惯法,并在此基础上分析了习惯法与国家法冲突的具体表现及原因,并从观念层面、立法层面及司法层面提出建议,以期实现白裤瑶婚姻习惯法与国家法之融合。

白裤瑶葬俗解读

白裤瑶人死后,一般不立即下葬,而要等到秋冬时节,举行砍牛、打铜鼓等仪式之后才下葬。葬礼规模无论大小,在悲哀的气氛中都可见喜庆的色彩,其葬礼的过程充分表达了白裤瑶人对灵魂归宿的理解。

广西白裤瑶服饰图案程式化解析

白裤瑶服饰保留了一套完整的手工制作技术,其服饰图案设计严谨、独特,制作精美,传承千年依旧历久弥新,极具现代审美,呈现少数民族完美的自然生活美学。白裤瑶服饰图案巧妙地运用了形式美法则,图案形式、色彩及传承的理念都包含了很多程式化特征,这些程式化特征既是白裤瑶先人的智慧,也是一代又一代白裤瑶妇女传承的精髓,对白裤瑶的传承和创新具有重要意义。

白裤瑶族的背饰文化解析

文章从造型艺术及文化内涵等方面对白裤瑶族的背饰文化进行了系统地解读,分析了其历史文化价值、美学价值,指出对白裤瑶族背饰文化的保护、传承、发展有助于纺织服装行业的发展和民族文化的推广。