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Screening and functional evaluation of the glucose-lowering active compounds of total saponins of Baibiandou(Lablab Semen Album) 被引量:2
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作者 HAN Jun ZHENG Qinfang +1 位作者 FANG Liangzi HUANG Xiaolong 《Digital Chinese Medicine》 2021年第3期229-240,共12页
Objective To screen forα-glucosidase inhibitor active compounds in the total saponins of Baibiandou(Lablab Semen Album)based on UHPLC-Q-Exactive Orbitrap MS technology and to evaluate its hypoglycemic activity in viv... Objective To screen forα-glucosidase inhibitor active compounds in the total saponins of Baibiandou(Lablab Semen Album)based on UHPLC-Q-Exactive Orbitrap MS technology and to evaluate its hypoglycemic activity in vivo.Methods Acarbose was used as the positive control,and the median inhibitory concentration(IC50)was used as the evaluation index ofα-glucosidase inhibitory activity to establish an in vitroα-glucosidase inhibition model.Further,UHPLC-Q-Exactive Orbitrap MS technology was used to screen and identify the active compounds ofα-glucosidase inhibitors in the total saponins of Baibiandou(Lablab Semen Album)in order to further verify the activity of the main active monomer and to perform homologous modeling and molecular docking of yeast-derivedα-glucosidase and human-derivedα-glucosidase,while the hypoglycemic activity was evaluated in diabetic mice.Results This study successfully identified 15 compounds with potentialα-glucosidase inhibitory activity,including Chikusetsusaponin IVa,from the total saponins of Baibiandou(Lablab Semen Album).Simultaneously,we verified the activity of the main active monomer Chikusetsusaponin IVa,and showed that it has strongα-glucosidase inhibitory activity.Theα-glucosidase inhibitory concentration IC50 was(565.2±1.026)μg/m L,and the IC50 of acarbose,which was lower than the positive control,was(706.6±1.058)μg/m L.The docking energies of Chikusetsusaponin IVa were–6.1 and–7.7 kcal/mol with yeast-derivedα-glucosidase and human-derivedα-glucosidase molecules,respectively.Both showed strong binding activity,and the levels of alanine aminotransaminase(ALT),aspartate aminotransaminase(AST),UREA,Creatinine(CREA),and cholesterol(CHO)were significantly decreased by Chikusetsusaponin IVa(P<0.05).In addition,it could repair damaged liver and pancreas cells of diabetic mice to some extent.Conclusion This study provides a basis for screeningα-glucosidase inhibitors and structural modifications of the total saponins of Baibiandou(Lablab Semen Album). 展开更多
关键词 baibiandou(lablab Semen Album) Total saponins UHPLC-Q-Exactive Orbitrap MS Α-GLUCOSIDASE Molecular docking Type 2 diabetic mice Chikusetsusaponin IVa
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腈吡螨酯对扁豆二斑叶螨的田间药效及安全性评价 被引量:1
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作者 陈秀 方朝阳 +3 位作者 高永东 黄兰淇 赵莉 张颂函 《农药科学与管理》 CAS 2024年第1期39-43,共5页
二斑叶螨是扁豆主要害虫之一,发生较重时可降低扁豆产量和品质。本研究评价了30%腈吡螨酯悬浮剂对扁豆二斑叶螨的田间药效及其对扁豆的安全性。田间使用有效成分量67.5、90、112.5 g/hm2,药后1 d三地的田间防效66.5%~85.9%、药后3 d 82.... 二斑叶螨是扁豆主要害虫之一,发生较重时可降低扁豆产量和品质。本研究评价了30%腈吡螨酯悬浮剂对扁豆二斑叶螨的田间药效及其对扁豆的安全性。田间使用有效成分量67.5、90、112.5 g/hm2,药后1 d三地的田间防效66.5%~85.9%、药后3 d 82.1%~92.3%、药后7 d 86.9%~95.2%、药后14 d 80.9%~96.1%,能有效防控扁豆二斑叶螨,具有较好的速效性和良好的持效性。作物安全性试验表明30%腈吡螨酯悬浮剂对扁豆安全。建议登记30%腈吡螨酯悬浮剂防治扁豆二斑叶螨,推荐使用有效成分量67.5~112.5 g/hm^(2)(制剂量15~25 mL/667m^(2)),于二斑叶螨发生初期施药1次。 展开更多
关键词 扁豆 二斑叶螨 腈吡螨酯 田间药效 安全性
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拉巴豆替代控制对美洲商陆根际土壤微环境的影响
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作者 乔国兰 孙镕基 +1 位作者 高文婧 陈桂华 《湖南农业科学》 2024年第6期28-31,37,共5页
为探究拉巴豆替代控制对美洲商陆根际土壤微环境的影响,分别测定了美洲商陆单种及与拉巴豆按不同比例混种时根际土壤细菌、真菌和放线菌数量,及土壤全氮、全磷、全钾、碱解氮、速效磷、速效钾和有机质含量。结果表明:混种拉巴豆显著降... 为探究拉巴豆替代控制对美洲商陆根际土壤微环境的影响,分别测定了美洲商陆单种及与拉巴豆按不同比例混种时根际土壤细菌、真菌和放线菌数量,及土壤全氮、全磷、全钾、碱解氮、速效磷、速效钾和有机质含量。结果表明:混种拉巴豆显著降低了美洲商陆根际土壤细菌数量、放线菌数量和所占比例、微生物总数、全磷、碱解氮、速效钾和有机质含量,显著提高了真菌所占比例,1∶3混种(S2L6)时细菌所占比例显著降低,1∶1(S4L4)和1∶3(S2L6)混种时真菌数量显著提高,全氮和速效磷含量显著降低。相关性分析表明根际土壤微生物数量和理化性质相互影响,由主成分分析综合排名可知1∶3混种时美洲商陆根际土壤肥力条件最差。综上所述,因为混种使美洲商陆根际土壤肥力降低,说明拉巴豆能够成功替代控制美洲商陆,1∶3混种最有利于美洲商陆的替代控制。 展开更多
关键词 美洲商陆 拉巴豆 替代控制 微生物数量 土壤理化性质
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基于ICP-MS/MS测定中药白扁豆中18种无机元素及其质量评价
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作者 戚鹏飞 王月玲 +4 位作者 邵长春 李洁 张晓萍 季申 朱仁愿 《中国医药导刊》 2024年第3期248-253,共6页
目的:采用串联四级杆电感耦合等离子体质谱法(ICP-MS/MS)测定中药白扁豆中18种无机元素的含量,结合化学计量学分析不同产地及性状差异白扁豆的质量情况。方法:采用超级微波消解处理样品后,采用串联四级杆电感耦合等离子体质谱法进行测定... 目的:采用串联四级杆电感耦合等离子体质谱法(ICP-MS/MS)测定中药白扁豆中18种无机元素的含量,结合化学计量学分析不同产地及性状差异白扁豆的质量情况。方法:采用超级微波消解处理样品后,采用串联四级杆电感耦合等离子体质谱法进行测定,对测定结果进行主成分分析及聚类分析。结果:测定的各无机元素线性关系良好;主成分分析得到6个主成分,累计方差贡献率达到83.959%;确定锰(Mn)、镍(Ni)、铜(Cu)、锶(Sr)、钴(Co)、硒(Se)、铷(Rb)、钡(Ba)、铬(Cr)、镉(Cd)、银(Ag)、锂(Li)、铍(Be)、钒(V)、铅(Pb)等15种元素为白扁豆的主要特征元素。结论:该方法操作简便、结果准确、灵敏度高,可用于中药白扁豆中无机元素的测定,为进一步研究中药白扁豆提供了参考。 展开更多
关键词 ICP-MS/MS 白扁豆 无机元素 主成分分析
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中药白扁豆基原考证及研究进展
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作者 戚鹏飞 王月玲 +4 位作者 李运 邵长春 邱国玉 张倩 李洁 《中国民族民间医药》 2024年第9期67-72,共6页
白扁豆是药食同原常用中药,用药历史悠久,本草类文献均有记载,历版中国药典均收载。但是,近年来市场流通白扁豆出现了性状差异品种,基于现有质量标准真伪鉴定及质量控制尚有欠缺,通过市场调研及样品收集,系统地查阅文献资料及现有研究... 白扁豆是药食同原常用中药,用药历史悠久,本草类文献均有记载,历版中国药典均收载。但是,近年来市场流通白扁豆出现了性状差异品种,基于现有质量标准真伪鉴定及质量控制尚有欠缺,通过市场调研及样品收集,系统地查阅文献资料及现有研究文献对白扁豆基原和质量控制方面的研究进行综述,为其本草考证及进一步质量控制研究提供宝贵依据。 展开更多
关键词 白扁豆 本草考证 质量标准 药理活性
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Molecular Diversity of Kenyan Lablab Bean (<i>Lablab purpureus</i>(L.) Sweet) Accessions Using Amplified Fragment Length Polymorphism Markers 被引量:3
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作者 Esther N. Kimani Francis N. Wachira Miriam G. Kinyua 《American Journal of Plant Sciences》 2012年第3期313-321,共9页
Lablab purpureus (L.) Sweet is a multipurpose legume that combines use as human food and animal feed in addition to serving as a cover crop for soil conservation. In this work, molecular diversity in Lablab purpureus ... Lablab purpureus (L.) Sweet is a multipurpose legume that combines use as human food and animal feed in addition to serving as a cover crop for soil conservation. In this work, molecular diversity in Lablab purpureus was assessed using amplified fragment length polymorphism markers on fifty Kenyan lablab accessions obtained from farmers’ fields and the Kenya National gene bank. One hundred and eighty polymorphic bands were revealed using fifteen selective primer pairs. The overall mean expected heterozygosity (He) for the five populations was 0.189. Estimates of components of molecular variance revealed that most of the genetic variation resided within populations (99%) and only 1% variance was among the populations, while Principal Coordinate Analysis showed an overlap between accessions from different geographic origins. The UPGMA cluster analysis generated from the distance matrix of the 50 assayed accessions, revealed low diversity among most of the accessions. The low diversity observed may be due to the narrow genetic base for breeding stocks, and extensive exchange of germplasm among smallholder farmers across the country. Results obtained from this study are discussed in light of the need to enhance the genetic management and improvement of this multipurpose crop species. 展开更多
关键词 lablab purpureus Molecular MARKERS AFLP Genetic Diversity
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Nutritional Quality of Mixed Silage of Lablab purpureus and Sweet Sorghum 被引量:4
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作者 Liu Hailin Deng Huifen +1 位作者 Xu Huaqin Sun Ao 《Animal Husbandry and Feed Science》 CAS 2017年第6期398-401,416,共5页
[ Objective ] The paper was to evaluate the fermentation quality of mixed silage of Lablab purpureus and sweet sorghum, and to find out the appropriate mixing ratio. [ Method] L. purpureus were mixed with sweet sorghu... [ Objective ] The paper was to evaluate the fermentation quality of mixed silage of Lablab purpureus and sweet sorghum, and to find out the appropriate mixing ratio. [ Method] L. purpureus were mixed with sweet sorghum at different proportions, to identify the sensory character and quality of silage. [ Result] The nutrient content of mixed silage of L. purpureus and sweet sorghum at different proportions decreased significantly at 30 - 60 d, while no significant changes were observed after 60 d. Mixed silage of L. purpureu.s and sweet sorghum had the best effort at the proportion of 3:7 ; followed by the proportion of 5: 5. These two proportions significantly improved dry matter (DM) content and effectively alleviated the crude protein (CP) loss of raw materials; significantly improved the contents of crude fiber and crude ash; and significantly reduce ammonia nitrogen/total nitrogen (AT/TN). [ Conclusion ] From the perspective of silage quality, the appropriate mixing ratio ofL. purpureus and sweet sorghum is 3:7 or 5:5. 展开更多
关键词 lablab purpureus (L.) Sweet Sweet sorghum Mixed silage Nutritional composition
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Research on saponin active compounds of Tuchao Baibiandouren for the treatment of type-2 diabetes based on UHPLC-Q-Exactive Orbitrap MS and network pharmacology 被引量:4
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作者 FANG Liangzi ZHENG Qinfang HAN Jun 《Digital Chinese Medicine》 2021年第1期19-31,共13页
Objective To explore the pharmacological mechanism of active saponin compounds of Tuchao Baibiandouren(Lablab Semen Album fried with earth,TCBBDR)in treating type 2 diabetes(T2DM)using UHPLC-Q-Exactive Orbitrap MS and... Objective To explore the pharmacological mechanism of active saponin compounds of Tuchao Baibiandouren(Lablab Semen Album fried with earth,TCBBDR)in treating type 2 diabetes(T2DM)using UHPLC-Q-Exactive Orbitrap MS and network pharmacology.Methods UHPLC-Q-Exactive Orbitrap MS was used for a qualitative analysis of saponin compounds in TCBBDR.PharmMapper and CTD were used to screen drug active compounds and disease targets,and an active compound-target network was constructed via Cytoscape 3.8.0.Molecular docking was applied with the drug active compounds and key targets using AutoDock Vina 1.1.2,and a trajectory for the molecular dynamics simulation was completed by GROMACS 2019-3.Results Sixteen saponin compounds were identified from TCBBDR,along with 292 saponin compoud targets and 792 T2DM targets.Through Venn analysis of target saponin constituents and T2DM related targets,a total of 91 intersection targets were screened out in the treatment of T2DM with saponin.The mean values of degree,betweenness centrality and closeness centrality were taken as the thresholds to screen out 22 key genes,among which 4 key proteins namely MAPK1,IGF1 EGFR,PIK3R1 were selected in the top 10 key genes.On this basis,the saponin active constituent-target-signaling pathway network was established.The Gene Ontology(GO)enrichment analysis showed that the related biological modules included activity of steroid hormone receptor,steroid binding,and insulin receptor binding,etc.;the related signaling pathways were EGFR,PI3K-Akt and MAPK,etc.;regulating signaling pathways like MAPK could induce the proliferation,inhibition and apoptosis of pancreaticβcells,increase the quantity of pancreaticβcells,improve the functions of pancreaticβcells and stimulate the insulin secretion.Docking experiment analysis showed that all selected saponin compounds could enter the active sites of targets and form 3–14 hydrogen bonds with residues of the active sites.Moreover,van der Waals forces were present between chemical compounds and active sites.By combining the docking binding energy,we determined that the chemical compounds showed strong binding energy to the targets.Conclusion TCBBDR exerts therapeutic effects on diabetes through multi-compound and multi-target collaboration.Specifically,saponin components mediate pathways including inflammatory reaction and signal transduction to treat T2DM by regulating several key proteins that interact with EGFR and a series of signaling pathways related to disease development. 展开更多
关键词 UHPLC-Q-Exactive Orbitrap MS Network pharmacology Tuchao baibiandouren(lablab Semen Album fried with earth TCBBDR) Type-2 diabetes Molecular docking Molecular dynamics simulation
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Nutritive Value and Silage Fermentation Characteristics of Forage Sorghum (Sorghum bicolor L.) Genotypes and Lablab (Lablab purpureus L.) Mixture
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作者 M. Rangappa Umesh Isaac Lepcha +2 位作者 Sultan Begna Prasanna Gowda Sangu Angadi 《American Journal of Plant Sciences》 2022年第6期723-733,共11页
This study was conducted to assess the fermentation and nutritive value of sorghum silage mixed with lablab at different proportions. The treatments consisted of a combination of two sorghum genotypes viz. “Brown mid... This study was conducted to assess the fermentation and nutritive value of sorghum silage mixed with lablab at different proportions. The treatments consisted of a combination of two sorghum genotypes viz. “Brown midrib” and “Brachytic dwarf” genotype of lablab and six population proportions viz. 150:0, 112.5:37.5, 75:75, 37.5:112.5, 0:150 and 150:150 × 10<sup>3</sup> plants&middot;ha<sup>-1</sup> sorghum genotypes and lablab, respectively totalling to 12 numbers. Sorghum genotypes and lablab were grown as monocrop and in intercropping systems in the same field. Forage sorghum was harvested at the late-dough stage and lablab at 20% bloom. They were cut and chopped together and ensiled. Lablab in the silage mixture was its actual contribution to the total forage mixture. For each mixture, a 1-L glass jar (mini-silo) was filled with 500 g of fresh material and replicated four times. Forage in mini silos was fermented for 60 days at room temperature (25&deg;C). Pre and post-silage dry samples were analysed for nutritive value and ensiled samples were analysed for fermentation characteristics. There was no significant difference in nutritive value between sorghum genotypes. The greatest impact of mixing lablab with sorghum genotypes was on crude protein (CP) and acid detergent fiber (ADF), but not on neutral detergent fiber (NDF). Across treatments, CP, ADF pH, lactic, and acetic acid concentrations increased as the proportion of LB was increased. The results indicated that lablab as an intercrop with sorghum for greater DM yield and forage and silage quality than respective monocrops. 展开更多
关键词 Brown Midrib Brachytic Dwarf Ensilage Forage Quality INTERCROPPING lablab SILAGE
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Differential Aphid Colony Establishment in <i>Dolichos lablab</i>Varieties Correlated with Some Plant Specific Factors That Impact on Aphid Fecundity
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作者 Hossain Ali Mondal Suvendu Kumar Roy +3 位作者 Lakshmi Hijam Moumita Chakraborty Puspendu Dutta Tapan Kumar Hath 《American Journal of Plant Sciences》 2017年第4期754-769,共16页
Aphid establishes colony in the selective plant parts like vine, leaf petiole, leaflet, inflorescence, and tender fruit in Dolichos lablab Linn but not the entire plant. In this study, the aphid colony establishment i... Aphid establishes colony in the selective plant parts like vine, leaf petiole, leaflet, inflorescence, and tender fruit in Dolichos lablab Linn but not the entire plant. In this study, the aphid colony establishment in vine is focused to understand the differential resistance response between two varieties. At the early stage of aphid infestation, the aphid colony establishment was significantly different between two genotypes (p value = 0.00) and abbreviated as “resistant” variety that supported lower aphid proliferation (mean value = 48.2 ± 2.2) and “susceptible” variety that supported comparatively higher aphid proliferation (mean value = 215.5 ± 16.9). The total aphid number was significantly different between the two varieties, realized at the early infestation stage when both “antixenosis” and “antibiosis” defense mechanisms were working on. Some plant specific factors like vine diameter, wet/ dry weight ratio of vine, phloem sap pressure, the compactness of the vine, wet/dry weight ratio of leaflet, length of leaf petiole, diameter of leaflet vein were identified as modulating factors. The impact of resistant variety on aphid was also investigated for better understanding of aphid defense mechanism. 展开更多
关键词 Dolichos lablab APHID COLONY DIFFERENTIAL VINE Diameter DIFFERENTIAL Wet/Dry Weight Ratio of VINE DIFFERENTIAL Phloem Pressure DIFFERENTIAL Compactness of the VINE DIFFERENTIAL APHID FECUNDITY DIFFERENTIAL Water Content in APHID
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Identification of Lablab Semen Album by DNA Barcode Technology
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作者 Huiming LUO Jian RAO +3 位作者 Bingyi XIAO Ping NIE Hai LIN Ye DING 《Medicinal Plant》 2017年第6期45-47,共3页
[Objectives] To identify ITS2 barcode of Lablab Semen Album and its adulterants,and provide a new method for the identification of Lablab Semen Album. [Methods] The ITS2 sequence was amplified by PCR and sequenced bi-... [Objectives] To identify ITS2 barcode of Lablab Semen Album and its adulterants,and provide a new method for the identification of Lablab Semen Album. [Methods] The ITS2 sequence was amplified by PCR and sequenced bi-directionally. After splicing by Codon Code Aligner,the data were processed with the aid MEGA software to construct the cluster dendrogram( neighbor-joining,NJ tree). [Results]The ITS2 sequence of Lablab Semen Album had length of 218 bp; the constructed cluster dendrogram indicated that all species were monophyletic and could be distinguished from other species. [Conclusions] The ITS2 barcode can be used for rapid identification of Lablab Semen Album and its adulterants and this experiment further verified that DNA barcode technology is effective in identification of traditional Chinese medicines. 展开更多
关键词 DNA BARCODE ITS2 lablab SEMEN ALBUM PCR AMPLIFICATION IDENTIFICATION
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基于ITS2序列对市售白扁豆的鉴别研究 被引量:1
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作者 毕武 聂平 +3 位作者 孙辉 丁野 张文娟 马双成 《药品评价》 CAS 2023年第3期281-285,共5页
目的了解不同来源市售白扁豆核糖体内部转录间隔区2(internal transcribed spacer 2,ITS2)序列信息,并基于该序列对其进行鉴定。方法收集云南、安徽、广东等地以及进口白扁豆31份,采用试剂盒法提取DNA,聚合酶链式反应(PCR)扩增ITS2序列... 目的了解不同来源市售白扁豆核糖体内部转录间隔区2(internal transcribed spacer 2,ITS2)序列信息,并基于该序列对其进行鉴定。方法收集云南、安徽、广东等地以及进口白扁豆31份,采用试剂盒法提取DNA,聚合酶链式反应(PCR)扩增ITS2序列后并测序,对峰图校对和拼接,与从GeneBank数据库下载的71条白扁豆及其混伪品ITS2序列进行综合分析,并构建基于邻接法的系统发育树,预测ITS2二级结构。结果31份市售白扁豆均测序成功,白扁豆ITS2条形码序列存在两个变异位点,基于ITS2条形码序列及其二级结构均可将白扁豆与其近缘混伪品区分。结论不同产地和来源白扁豆ITS2条形码序列无明显差异,ITS2条形码序列可以用于白扁豆的真伪鉴别。 展开更多
关键词 白扁豆 DNA条形码 鉴别
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宁夏干旱区滴灌条件下拉巴豆不同播种量与甜高粱混播饲草生产性能研究 被引量:2
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作者 王腾飞 王斌 +5 位作者 邓建强 李满有 倪旺 冯琴 妥昀昀 兰剑 《草业学报》 CSCD 北大核心 2023年第3期30-40,共11页
采用合理的种植方式能够有效缓解宁夏干旱区饲草产量低、品质差问题,本研究在滴灌条件下设置不同播种量(S_(0):0 kg·hm^(-2),S_(1):16.5 kg·hm^(-2),S_(2):33.0 kg·hm^(-2),S_(3):49.5 kg·hm^(-2),S_(4):66.0 kg... 采用合理的种植方式能够有效缓解宁夏干旱区饲草产量低、品质差问题,本研究在滴灌条件下设置不同播种量(S_(0):0 kg·hm^(-2),S_(1):16.5 kg·hm^(-2),S_(2):33.0 kg·hm^(-2),S_(3):49.5 kg·hm^(-2),S_(4):66.0 kg·hm^(-2))拉巴豆与甜高粱混播,对甜高粱农艺性状和混播草地生产性能以及牧草营养品质进行综合分析。2年试验结果表明,拉巴豆播种量对甜高粱株高、茎粗、茎秆1~4节强度和茎秆5~7节强度及混播草地干草产量、粗蛋白产量、粗蛋白含量、中性洗涤纤维含量、酸性洗涤纤维含量和相对饲喂价值有显著影响(P<0.05),其中甜高粱株高、茎粗、混播草地干草产量和粗蛋白产量随拉巴豆播种量的增加呈先增加后降低趋势,草地总干草产量、粗蛋白产量和相对饲喂价值均在S3处理下达到最大,分别为32.12 t·hm^(-2)、4.13 t·hm^(-2)和117.30;混合牧草粗蛋白含量在S4处理下最大,达到12.31%。经主成分(PCA)综合分析可知,拉巴豆与甜高粱混播草地的株高、茎粗、粗蛋白产量、总干草产量、茎秆1~4节强度和相对饲喂价值贡献率较大,综合排名由高到低为S_(3)>S_(2)>S_(4)>S_(1)>S_(0)。综合混播草地生产性能与营养品质,建议在宁夏干旱区滴灌条件下拉巴豆与甜高粱混播的最佳播种量为49.5 kg·hm^(-2)。 展开更多
关键词 拉巴豆 甜高粱 生产性能 营养品质 综合评价
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紫色土坡耕地地埂草篱根系土壤抗蚀与抗冲性能特征研究 被引量:1
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作者 李鸿 谌芸 +2 位作者 刘枭宏 刘有斌 都艺芝 《草业学报》 CSCD 北大核心 2023年第11期40-52,共13页
为探明紫色土坡耕地不同地埂草篱根系土壤抗蚀和抗冲性的差异,以拉巴豆和紫花苜蓿地埂草篱为研究对象,以未种植草篱地埂为对照(CK),系统性地研究了土壤抗蚀和抗冲性能对草篱种类的响应,阐明了二者与根系形态特征及土壤理化性质间的耦合... 为探明紫色土坡耕地不同地埂草篱根系土壤抗蚀和抗冲性的差异,以拉巴豆和紫花苜蓿地埂草篱为研究对象,以未种植草篱地埂为对照(CK),系统性地研究了土壤抗蚀和抗冲性能对草篱种类的响应,阐明了二者与根系形态特征及土壤理化性质间的耦合关系。结果表明:1)拉巴豆整株根系长度、根表面积和土壤有机质含量显著高于紫花苜蓿,且土壤含水率、总孔隙度和非毛管孔隙度显著高于紫花苜蓿和CK(P<0.05);2)土壤水稳性团聚体平均质量直径、平均几何直径及微团聚体团聚度大小表现为拉巴豆>紫花苜蓿,水稳性团聚体结构破坏率和微团聚体分散系数大小表现为紫花苜蓿>拉巴豆;3)拉巴豆土壤抗冲指数及抗冲指数变化值均大于紫花苜蓿,抗冲指数是冲刷时间的对数函数,抗冲指数变化值是冲刷时间的二次函数;4)冗余分析的结果表明整株分叉数、根尖数、土壤有机质及非毛管孔隙度对土壤抗蚀和抗冲性能的影响较大,逐步回归的结果表明>0.5 mm的水稳性团聚体结构破坏率是影响土壤抗冲指数的决定因子。综上,拉巴豆地埂草篱土壤抗蚀和抗冲性能优于紫花苜蓿。本研究可为紫色土坡耕地地埂草篱技术的推广应用提供理论依据。 展开更多
关键词 拉巴豆 地埂草篱 团聚体 抗冲指数 土壤侵蚀
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象草与白花扁豆对娟姗牛的饲喂效果研究 被引量:1
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作者 邓素媛 易显凤 +5 位作者 丘金花 黄光云 赖大伟 曹树威 李创举 黄志朝 《家畜生态学报》 北大核心 2023年第7期34-39,共6页
为探讨象草在娟姗牛养殖中的饲用价值以及利用方式,该试验选取15头娟姗牛,随机分成3组,试验1组饲喂桂牧1号杂交象草(P.purpureum.cv.Mott×(P.americanum×P.purureum)cv.GuimuNo.1,GuimuNo.1),试验2组饲喂紫色象草(P.purpureum... 为探讨象草在娟姗牛养殖中的饲用价值以及利用方式,该试验选取15头娟姗牛,随机分成3组,试验1组饲喂桂牧1号杂交象草(P.purpureum.cv.Mott×(P.americanum×P.purureum)cv.GuimuNo.1,GuimuNo.1),试验2组饲喂紫色象草(P.purpureum Schumabcv.Purple,Purple),试验3组饲喂70%桂牧1号杂交象草(GuimuNo.1)+30%白花扁豆(Lablabpurpureus),粗草料均为鲜草,正式期92d,分析各组草料对娟姗牛采食量、日增重、料肉比、利润以及血液生理生化指标的影响。结果表明:各象草品种中,紫色象草组的平均日增重为489.13g/d,料肉比为13.38,平均每头牛利润为523.66元,比桂牧1号杂交象草组日增重提高了0.75%、料肉比降低了1.18%、利润提高了3.25%。桂牧1号杂交象草+白花扁豆组的生长性能以及利润等方面效果最好,比桂牧1号杂交象草组平均日增重提高了4.48%,耗料量减少14.25%,每头牛利润增加79.51元。各组娟姗牛血液指标除了紫色象草组血红蛋白显著增高(P<0.05)、谷丙转氨酶显著低于其他各组外(P<0.05),其他指标差异不显著(P>0.05),各组粗草料对娟姗牛血液无不良影响。可见,紫色象草可作为育肥牛优质牧草来源,并且象草在肉牛养殖中与白花扁豆混搭配效果更佳。 展开更多
关键词 象草 白花扁豆 娟姗牛 生长性能 血液指标
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施氮对高丹草-扁豆套作系统产量、品质和氮素利用效率的影响 被引量:1
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作者 周丽 付薇 +5 位作者 覃涛英 孟军江 桂永清 任建华 韩永芬 丁磊磊 《草地学报》 CAS CSCD 北大核心 2023年第6期1754-1761,共8页
为探讨施氮量对高丹草-扁豆套作系统饲草产量、品质以及氮肥利用率的影响,本试验采用随机区组设计,主因素设高丹草(Sorghum bicolor×S.sudanense)/扁豆(Dolichos Lablab)套作(I)、高丹草(S)和扁豆(L)单作3种种植模式,副因素设0 kg&... 为探讨施氮量对高丹草-扁豆套作系统饲草产量、品质以及氮肥利用率的影响,本试验采用随机区组设计,主因素设高丹草(Sorghum bicolor×S.sudanense)/扁豆(Dolichos Lablab)套作(I)、高丹草(S)和扁豆(L)单作3种种植模式,副因素设0 kg·hm^(-2)(N 0,CK),180 kg·hm^(-2)(N 180),260 kg·hm^(-2)(N 260),340 kg·hm^(-2)(N 340)4个氮肥水平。结果表明:施氮有利于提高套作混合饲草的鲜/干产量和粗蛋白含量,与对照差异显著(P<0.05)。在N 260时套作混合饲草的产量、粗蛋白含量和氮肥利用效率达到最高,且显著高于单作高丹草(P<0.05)。施氮增加了混合饲草的粗纤维、中/酸性洗涤纤维含量,但在同一施氮处理下混合饲草的粗纤维、中/酸性洗涤纤维含量显著低于单作高丹草(P<0.05)。根据隶数函数综合评价表明,施氮肥260 kg·hm^(-2)时高丹草-扁豆套作系统混合饲草的品质最佳。 展开更多
关键词 高丹草/扁豆套作 施氮量 营养品质 氮肥利用率
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液相色谱-串联质谱法对云南省白扁豆中多种农残的质量检测分析 被引量:3
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作者 邓淙友 王闽予 +4 位作者 罗文安 谢文凯 官永河 吴辉强 曲丽媛 《按摩与康复医学》 2023年第1期76-80,共5页
目的:作为药食两用的经济作物,豆科植物白扁豆(Dolichos lablab L.,DLL)在种植过程中无法避免农药使用和残留。随着国家对食品安全问题的日渐重视,农药残留监控体系的建立成为现阶段必不可少的任务,而高分辨率质谱监控体系已经成为痕量... 目的:作为药食两用的经济作物,豆科植物白扁豆(Dolichos lablab L.,DLL)在种植过程中无法避免农药使用和残留。随着国家对食品安全问题的日渐重视,农药残留监控体系的建立成为现阶段必不可少的任务,而高分辨率质谱监控体系已经成为痕量农药监测的主要研究方法。本文对云南省的15个产地所产的白扁豆的522种农药残留进行检查,并分析了检出农药的膳食风险,为白扁豆的食用、售卖和种植提出理论依据。方法:采用固态萃取-UPLC-MS/MS联用技术,通过离子对监测方法对药典规定的522种习用农药建立了监测体系。结果:在白扁豆中,药典规定的522农药中有18种被检测出残留,分别涵盖了杀虫剂、除草剂、杀菌剂、有机杀螨剂、植物生长调节剂和杀鼠剂,其中:氧甲拌磷(杀虫剂)在各批次中均有检出,氨氟灵(除草剂)在13批次的白扁豆中均有检出,鼠立死(杀鼠剂)在其中9批次的白扁豆中均有检出。结论:检出的18种农药均处于较低的风险水平,但为考虑食品安全性,仍需对农户中氧甲拌磷的使用加以监控。 展开更多
关键词 白扁豆 液相色谱-串联质谱法 农药残留
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白扁豆和瓜蒌子药材粉末饮片煎煮工艺研究
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作者 海月 朱敏 +3 位作者 钱芳 陆超 吴磊 束雅春 《中国药业》 CAS 2023年第16期63-67,共5页
目的优化白扁豆、瓜蒌子药材粉末饮片的煎煮工艺。方法采用高效液相色谱(HPLC)法检测白扁豆、瓜蒌子药材粉末饮片中核苷类成分的含量。以核苷类成分总含量为评价指标,以粉碎粒度、提取时间、提取次数为考察因素,优化煎煮工艺,并验证。... 目的优化白扁豆、瓜蒌子药材粉末饮片的煎煮工艺。方法采用高效液相色谱(HPLC)法检测白扁豆、瓜蒌子药材粉末饮片中核苷类成分的含量。以核苷类成分总含量为评价指标,以粉碎粒度、提取时间、提取次数为考察因素,优化煎煮工艺,并验证。结果胞嘧啶、尿嘧啶、尿苷、鸟苷、腺苷进样量分别在0.0342~0.3423μg,0.0375~0.4569μg,0.0383~0.3831μg,0.0415~0.4146μg,0.0454~0.4538μg范围内与峰面积线性关系良好(r=1.0000);精密度、稳定性、重复性试验结果的RSD均小于2.0%;白扁豆药材粉末饮片样品中胞嘧啶、尿嘧啶、尿苷、腺苷平均加样回收率分别为97.55%,101.25%,98.87%,99.46%,RSD分别为1.84%,2.62%,2.22%,1.86%(n=9);瓜蒌子药材粉末饮片样品中胞嘧啶、尿嘧啶、尿苷、鸟苷、腺苷平均加样回收率分别为98.72%,100.80%,99.16%,99.26%,103.30%,RSD分别为4.84%,4.46%,2.12%,6.11%,4.03%(n=9)。白扁豆、瓜蒌子药材粉末饮片的最优煎煮工艺为,将药材饮片打成粗粉(后者需过2号筛),煎煮2次,每次20 min。结论本研究中优化所得工艺可为药材粉末饮片煎煮的规范化研究提供参考。 展开更多
关键词 白扁豆 瓜蒌子 核苷类成分 药材粉末饮片 煎煮工艺
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白扁豆淀粉性质的研究 被引量:11
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作者 郑琳 赵海泉 《安徽农业大学学报》 CAS CSCD 北大核心 2010年第2期214-217,共4页
研究白扁豆淀粉的颗粒和糊的性质。结果表明,白扁豆淀粉颗粒主要呈椭圆形、表面光滑,粒径范围在9-29μm,长轴平均粒径19μm;偏光十字明显,有的呈"X"型,有的呈垂直十字,较小的颗粒出现偏光盲区;X—光衍射图样为C型;淀粉的糊化温度为84.... 研究白扁豆淀粉的颗粒和糊的性质。结果表明,白扁豆淀粉颗粒主要呈椭圆形、表面光滑,粒径范围在9-29μm,长轴平均粒径19μm;偏光十字明显,有的呈"X"型,有的呈垂直十字,较小的颗粒出现偏光盲区;X—光衍射图样为C型;淀粉的糊化温度为84.0-94.6℃,峰值粘度为146 mPa.s,糊的冷、热稳定性好,凝胶性强,且淀粉的冻融稳定性好;直链淀粉含量为39.1%。 展开更多
关键词 白扁豆 淀粉 性质
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白扁豆多糖对正常小鼠体内抗氧化和免疫实验研究 被引量:24
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作者 弓建红 许小华 +2 位作者 王俊敏 杜绍亮 杨云 《食品工业科技》 CAS CSCD 北大核心 2010年第9期337-338,共2页
采用正常小鼠进行体内抗氧化和免疫实验,通过测定小鼠血清中超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)的活力,来评价其抗氧化能力;通过观察正常小鼠腹腔巨噬细胞吞噬功能和小鼠血清溶血素形成的影响,来评价其免疫活性。结果表明... 采用正常小鼠进行体内抗氧化和免疫实验,通过测定小鼠血清中超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)的活力,来评价其抗氧化能力;通过观察正常小鼠腹腔巨噬细胞吞噬功能和小鼠血清溶血素形成的影响,来评价其免疫活性。结果表明,白扁豆多糖可使SOD、GSH-Px活力升高,可显著提高正常小鼠腹腔巨噬细胞的吞噬百分率和吞噬指数,促进溶血素形成。 展开更多
关键词 白扁豆多糖 抗氧化活性 免疫活性
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