The extract from Hedyotis diffusae was used to treat tobacco plants infected by TMV before and after incidence. The effect of the changes of some physiological and biochemical indices related with the virus-resistance...The extract from Hedyotis diffusae was used to treat tobacco plants infected by TMV before and after incidence. The effect of the changes of some physiological and biochemical indices related with the virus-resistance in tobacco leaves on tobacco quality ware determined. The resuits showed that H. diffusae extract could remarkably increase the total phenol content in tobacco leaves ,strength the activity of phenylalanine ammonialyase （ PAL） , improve the membrane permeability and tobacco quality. Prevention treatment was better than therapy treatment. In conclusion, H. diffusae extract could induce plants to produce disease resistance and strength tha antiviral capability of tobacco against TMV.展开更多
The whole plant with root ofHedyotis diffusa Willd. （AE） has been demonstrated to have anti-inflammatory, antioxidant, and anti-bacterial activities. In our study, we aim to examine the anti-tumor effect of alcoholi...The whole plant with root ofHedyotis diffusa Willd. （AE） has been demonstrated to have anti-inflammatory, antioxidant, and anti-bacterial activities. In our study, we aim to examine the anti-tumor effect of alcoholic extract of AE in mice implanted with sarcoma S180 cells （SBT mice）. We also compared the immune system function and the life span of SBT mice with that of control mice. We found that AE displayed a significant inhibitory effect on solid tumor growth, as well as increased the life span. At the dose of 10 mg/kg body weight, both tumor weight and volume were decreased significantly. We also measured several immune fimction markers, inlcuding spleen index （SI）, thymus index （TI） and parameters of hematological. We observed no reduce in these markers, indicatine that AE could inhibit tumor ~rowth without affectino immune function in SBT ,nice.展开更多
目的 基于网络药理学及分子对接技术,探究白花蛇舌草和半枝莲药治疗卵巢癌的作用机制。并对药对活性成分槲皮素进行体外细胞实验,验证其对卵巢癌的作用。方法 从中药系统药理学数据库与分析平台(Traditional Chinese Medicine Systems P...目的 基于网络药理学及分子对接技术,探究白花蛇舌草和半枝莲药治疗卵巢癌的作用机制。并对药对活性成分槲皮素进行体外细胞实验,验证其对卵巢癌的作用。方法 从中药系统药理学数据库与分析平台(Traditional Chinese Medicine Systems Pharmacology,TCMSP)数据库筛选白花蛇舌草和半枝莲的活性成分并找出对应的靶点;在GeneCards数据库中检索出卵巢癌相关靶点;用jvenn在线作图工具得到药对和疾病相同的靶点交集;将交集靶点导入STRING平台,构建蛋白质-蛋白质相互作用(protein-protein interaction network,PPI)关系,用Cytoscape做出白花蛇舌草和半枝莲药对治疗卵巢癌的主要靶点图,用Metascape数据库对主要靶点进行基因本体(GO)富集分析和京都基因与基因组百科全书(KEGG)通路富集分析。用分子对接对结果进行验证。并对白花蛇舌草-半枝莲活性成分槲皮素进行CCK-8细胞增殖实验和流式细胞术检测细胞凋亡实验。结果 与卵巢癌相关的白花蛇舌草-半枝莲药对成分-靶点网络中包含30种成分、109个靶点。其相关性位于前3位的候选化合物分子为槲皮素、木犀草素、β-谷甾醇。这些成分通过AKT1、BCL2L1、CASP3、CASP7等信号蛋白来抑制卵巢癌细胞的生长和转移,涉及信号通路有癌症的途径、乙型肝炎、脂质和动脉粥样硬化、AGE-RAGE信号通路在糖尿病并发症中的应用、PI3K-AKT信号通路等。分子对接结果显示核心成分与核心靶点均有较好的结合能力。不同浓度槲皮素可明显抑制卵巢癌细胞增殖,且浓度越高细胞抑制率越高,细胞凋亡率也随浓度增高,数据结果差异有统计学意义(P<0.01)。结论 从网络药理学和分子对接技术得出白花蛇舌草和半枝莲药对可通过多成分、多靶点、多通路治疗卵巢癌的作用机制。槲皮素能有效抑制卵巢癌细胞并促进其凋亡。展开更多
The Hedyotis diffusa Willd herbal compounds(HDWHCs)are commonly used as Chinese medicine to treat cancer patients with established clinical therapeutic efficacy in China.However,the underlying mechanisms remain to be ...The Hedyotis diffusa Willd herbal compounds(HDWHCs)are commonly used as Chinese medicine to treat cancer patients with established clinical therapeutic efficacy in China.However,the underlying mechanisms remain to be elucidated.In this study,we used freeze-dried powder of the water extracts of HDWHCs to investigate the potential mechanisms of HDWHCs in cancer treatment.HDWHCs treatment significantly inhibited vascular endothelial growth factor(VEGF)mRNA levels and VEGF transcriptional activation in cancer cells.HDWHCs also had a remarkable inhibitory effect on the expression of hypoxia-inducible factor 1alpha(HIF-1alpha).Forced expression of HIF-1αrestored VEGF transcriptional activation inhibited by HDWHCs,indicating that HDWHCs suppressed VEGF expression through decreasing HIF-1alpha expression.Moreover,HDWHCs inhibited cyclooxygenase-2(COX-2)expression,and overexpression of HIF-1alpha restored HDWHCs’inhibitory effect on COX-2 at transcriptional level.These findings may provide better understanding of HDWHCs’anti-cancer mechanism in cancer treatment.展开更多
Background:Abnormal proliferation of T cells plays an essential role in the pathogenesis of Systemic lupus erythematosus(SLE).The pharmaceutical effect of Hedyotis Diffusa Willd(HDW)on SLE has been investigated previo...Background:Abnormal proliferation of T cells plays an essential role in the pathogenesis of Systemic lupus erythematosus(SLE).The pharmaceutical effect of Hedyotis Diffusa Willd(HDW)on SLE has been investigated previously.Nevertheless,the biomedical mechanism is still left unclear.Objective:This study has been arranged to evaluate the therapeutic effect of the ethyl acetate fraction of HDW(EAHDW)on lupus mice and explore the potential therapeutic mechanism.Methods:EAHDW was prepared with 80%ethanol reflex extraction followed by successive extraction,and ana-lyzed with HPLC and UPLC-Q/TOP-MS.The potential targets and STAT3 affinity regulators were predicted with network pharmacology.The pharmaceutic effect of EAHDW was studied with MRL/lpr mice.Cytokines and au-toantibodies were quantified with ELISA assays.The pathological damage of glomerulus and STAT3 expression in the kidney was detected with histochemical and immunohistochemical techniques.The cell cycle properties in cell proliferation were identified with the flow cytometry.The western blot and dual-Luciferase reporter assay were applied to evaluate translational and transcriptional activity of STAT3,respectively.Results:In this study,the extraction ratio of EAHDW was 2.7±1%,in which 19 ingredients were identified.Network pharmacological analysis showed that the target genes of EAHDW were highly focused on influencing the abnormal T cell proliferation in SLE.EAHDW showed the beneficial effects on pathological changes and STAT3 expression in the glomerulus of lupus mice,and the levels of cytokines and autoantibodies in serum.In cytological study,EAHDW treatment attenuated the transcription and phosphorylation of STAT3,which inhibited T cell proliferation by prolonged S-phase of the cell cycle.A total of 5 compounds in EAHDW exhibited high docking affinity to the DNA-binding site of STAT3.Conclusion:EAHDW could reduce the inflammatory response and inhibit the proliferation of T cells by interfering with the STAT3 signaling pathway,thereby playing a therapeutic effect on SLE.展开更多
基金Supported by Yunnan Pu'er Tobacco Company Project~~
文摘The extract from Hedyotis diffusae was used to treat tobacco plants infected by TMV before and after incidence. The effect of the changes of some physiological and biochemical indices related with the virus-resistance in tobacco leaves on tobacco quality ware determined. The resuits showed that H. diffusae extract could remarkably increase the total phenol content in tobacco leaves ,strength the activity of phenylalanine ammonialyase （ PAL） , improve the membrane permeability and tobacco quality. Prevention treatment was better than therapy treatment. In conclusion, H. diffusae extract could induce plants to produce disease resistance and strength tha antiviral capability of tobacco against TMV.
文摘The whole plant with root ofHedyotis diffusa Willd. （AE） has been demonstrated to have anti-inflammatory, antioxidant, and anti-bacterial activities. In our study, we aim to examine the anti-tumor effect of alcoholic extract of AE in mice implanted with sarcoma S180 cells （SBT mice）. We also compared the immune system function and the life span of SBT mice with that of control mice. We found that AE displayed a significant inhibitory effect on solid tumor growth, as well as increased the life span. At the dose of 10 mg/kg body weight, both tumor weight and volume were decreased significantly. We also measured several immune fimction markers, inlcuding spleen index （SI）, thymus index （TI） and parameters of hematological. We observed no reduce in these markers, indicatine that AE could inhibit tumor ~rowth without affectino immune function in SBT ,nice.
文摘目的 基于网络药理学及分子对接技术,探究白花蛇舌草和半枝莲药治疗卵巢癌的作用机制。并对药对活性成分槲皮素进行体外细胞实验,验证其对卵巢癌的作用。方法 从中药系统药理学数据库与分析平台(Traditional Chinese Medicine Systems Pharmacology,TCMSP)数据库筛选白花蛇舌草和半枝莲的活性成分并找出对应的靶点;在GeneCards数据库中检索出卵巢癌相关靶点;用jvenn在线作图工具得到药对和疾病相同的靶点交集;将交集靶点导入STRING平台,构建蛋白质-蛋白质相互作用(protein-protein interaction network,PPI)关系,用Cytoscape做出白花蛇舌草和半枝莲药对治疗卵巢癌的主要靶点图,用Metascape数据库对主要靶点进行基因本体(GO)富集分析和京都基因与基因组百科全书(KEGG)通路富集分析。用分子对接对结果进行验证。并对白花蛇舌草-半枝莲活性成分槲皮素进行CCK-8细胞增殖实验和流式细胞术检测细胞凋亡实验。结果 与卵巢癌相关的白花蛇舌草-半枝莲药对成分-靶点网络中包含30种成分、109个靶点。其相关性位于前3位的候选化合物分子为槲皮素、木犀草素、β-谷甾醇。这些成分通过AKT1、BCL2L1、CASP3、CASP7等信号蛋白来抑制卵巢癌细胞的生长和转移,涉及信号通路有癌症的途径、乙型肝炎、脂质和动脉粥样硬化、AGE-RAGE信号通路在糖尿病并发症中的应用、PI3K-AKT信号通路等。分子对接结果显示核心成分与核心靶点均有较好的结合能力。不同浓度槲皮素可明显抑制卵巢癌细胞增殖,且浓度越高细胞抑制率越高,细胞凋亡率也随浓度增高,数据结果差异有统计学意义(P<0.01)。结论 从网络药理学和分子对接技术得出白花蛇舌草和半枝莲药对可通过多成分、多靶点、多通路治疗卵巢癌的作用机制。槲皮素能有效抑制卵巢癌细胞并促进其凋亡。
基金This work was supported by grants from the National Natural Science Foundation of China(Grant Nos.30470361,30570962,and 30871296)from Nanjing Medical University(No.08NMUM007).
文摘The Hedyotis diffusa Willd herbal compounds(HDWHCs)are commonly used as Chinese medicine to treat cancer patients with established clinical therapeutic efficacy in China.However,the underlying mechanisms remain to be elucidated.In this study,we used freeze-dried powder of the water extracts of HDWHCs to investigate the potential mechanisms of HDWHCs in cancer treatment.HDWHCs treatment significantly inhibited vascular endothelial growth factor(VEGF)mRNA levels and VEGF transcriptional activation in cancer cells.HDWHCs also had a remarkable inhibitory effect on the expression of hypoxia-inducible factor 1alpha(HIF-1alpha).Forced expression of HIF-1αrestored VEGF transcriptional activation inhibited by HDWHCs,indicating that HDWHCs suppressed VEGF expression through decreasing HIF-1alpha expression.Moreover,HDWHCs inhibited cyclooxygenase-2(COX-2)expression,and overexpression of HIF-1alpha restored HDWHCs’inhibitory effect on COX-2 at transcriptional level.These findings may provide better understanding of HDWHCs’anti-cancer mechanism in cancer treatment.
文摘Background:Abnormal proliferation of T cells plays an essential role in the pathogenesis of Systemic lupus erythematosus(SLE).The pharmaceutical effect of Hedyotis Diffusa Willd(HDW)on SLE has been investigated previously.Nevertheless,the biomedical mechanism is still left unclear.Objective:This study has been arranged to evaluate the therapeutic effect of the ethyl acetate fraction of HDW(EAHDW)on lupus mice and explore the potential therapeutic mechanism.Methods:EAHDW was prepared with 80%ethanol reflex extraction followed by successive extraction,and ana-lyzed with HPLC and UPLC-Q/TOP-MS.The potential targets and STAT3 affinity regulators were predicted with network pharmacology.The pharmaceutic effect of EAHDW was studied with MRL/lpr mice.Cytokines and au-toantibodies were quantified with ELISA assays.The pathological damage of glomerulus and STAT3 expression in the kidney was detected with histochemical and immunohistochemical techniques.The cell cycle properties in cell proliferation were identified with the flow cytometry.The western blot and dual-Luciferase reporter assay were applied to evaluate translational and transcriptional activity of STAT3,respectively.Results:In this study,the extraction ratio of EAHDW was 2.7±1%,in which 19 ingredients were identified.Network pharmacological analysis showed that the target genes of EAHDW were highly focused on influencing the abnormal T cell proliferation in SLE.EAHDW showed the beneficial effects on pathological changes and STAT3 expression in the glomerulus of lupus mice,and the levels of cytokines and autoantibodies in serum.In cytological study,EAHDW treatment attenuated the transcription and phosphorylation of STAT3,which inhibited T cell proliferation by prolonged S-phase of the cell cycle.A total of 5 compounds in EAHDW exhibited high docking affinity to the DNA-binding site of STAT3.Conclusion:EAHDW could reduce the inflammatory response and inhibit the proliferation of T cells by interfering with the STAT3 signaling pathway,thereby playing a therapeutic effect on SLE.
