In this study, we induced cerebral infarction in rats by occluding the right middle cerebral artery, and tested the effects of electroacupuncture at the Baihui acupoint （DU 20）. Motor and sensory function was tested...In this study, we induced cerebral infarction in rats by occluding the right middle cerebral artery, and tested the effects of electroacupuncture at the Baihui acupoint （DU 20）. Motor and sensory function was tested using Garcia’s scale and motor weakness grading, and the expression of vascular endothelial growth factor in the brain was quantified using immunoblotting and immunohistochemistry. We found that scalp electroacupuncture at DU 20 significantly improved motor performance and sensory function in rats with stroke, and this was accompanied by an increased expression of vascular endothelial growth factor in the ischemic brain tissue and peri-ischemic area. In addition, Pearson correlation analysis showed that the improvements in functional recovery were correlated with the increased expression of vascular endothelial growth factor.展开更多
Neuro D plays a key regulatory effect on differentiation of neural stem cells into mature neurons in the brain.Thus,we assumed that electroacupuncture at Baihui（DU20） acupoint in newborn rats exposed to in utero fet...Neuro D plays a key regulatory effect on differentiation of neural stem cells into mature neurons in the brain.Thus,we assumed that electroacupuncture at Baihui（DU20） acupoint in newborn rats exposed to in utero fetal distress would influence expression of Neuro D.Electroacupuncture at Baihui was performed for 20 minutes on 3-day-old（Day 3） newborn Sprague-Dawley rats exposed to in utero fetal distress;electroacupuncture parameters consisted of sparse and dense waves at a frequency of 2–10 Hz.Real-time fluorescent quantitative PCR results demonstrated that m RNA expression of Neuro D,a molecule that indicates Neuro D,increased with prolonged time in brains of newborn rats,and peaked on Day 22.The level of m RNA expression was similar between Day 16 and Day 35.These findings suggest that electro acupuncture at Baihui acupoint could effectively increase m RNA expression of molecules involved in Neuro D in the brains of newborn rats exposed to in utero fetal distress.展开更多
【目的】运用数据挖掘技术分析针刺治疗中风后失眠的选穴规律。【方法】通过检索中国期刊全文数据库(CNKI)、万方学术期刊全文数据库(Wanfang)、维普中文科技期刊数据库(VIP)、中国生物医学文献数据库(CBM),美国医学在线(PubMed)、核心...【目的】运用数据挖掘技术分析针刺治疗中风后失眠的选穴规律。【方法】通过检索中国期刊全文数据库(CNKI)、万方学术期刊全文数据库(Wanfang)、维普中文科技期刊数据库(VIP)、中国生物医学文献数据库(CBM),美国医学在线(PubMed)、核心合集数据库(Web of Science)以及荷兰医学文献数据库(Embase)等各大数据库,选取建库以来至2022年10月有关针刺治疗中风后失眠的临床研究文献,应用SPSS Statistic及SPSS Modeler构建数据库,采用关联规则分析及聚类分析,探究针刺治疗中风后失眠针刺处方选穴的规律。【结果】共纳入文献117篇,针刺处方119个,使用腧穴116种,腧穴总使用频次为887次。(1)穴位分析结果显示使用频次较高的腧穴为:百会、三阴交、内关、神庭、神门、四神聪。(2)选经以督脉、足太阳膀胱经、任脉为主,并且注重经外奇穴的使用。临床针刺部位主要选取头项、四肢部。(3)特定穴使用频次最高的为原穴、八脉交会穴和五输穴中的“输穴”。(4)关联规则分析显示,针刺治疗中风后失眠的核心腧穴组为百会-神庭-三阴交。(5)高频穴位聚类获得三个聚类群,第1类:百会;第2类:四神聪、内关、三阴交、神门、神庭;第3类:申脉、中脘、足三里、太冲、印堂、安眠、太溪、气海、关元、水沟。【结论】针刺治疗中风后失眠取穴方面多以督脉和头项部取穴为主;核心穴位为百会-神庭-三阴交;治则治法多遵循“引阳入阴,调整阴阳平衡”的原则。展开更多
基金the Incheon St. Mary’s Hospital of the Catholic University of Korea, through the Clinical Research Laboratory Foundation Program, Korea Health 21 R&D Project, No. A092058, and WCU Neurocytomics
文摘In this study, we induced cerebral infarction in rats by occluding the right middle cerebral artery, and tested the effects of electroacupuncture at the Baihui acupoint （DU 20）. Motor and sensory function was tested using Garcia’s scale and motor weakness grading, and the expression of vascular endothelial growth factor in the brain was quantified using immunoblotting and immunohistochemistry. We found that scalp electroacupuncture at DU 20 significantly improved motor performance and sensory function in rats with stroke, and this was accompanied by an increased expression of vascular endothelial growth factor in the ischemic brain tissue and peri-ischemic area. In addition, Pearson correlation analysis showed that the improvements in functional recovery were correlated with the increased expression of vascular endothelial growth factor.
基金supported by the Natural Science Foundation of Fujian Province of China,No.2015J01133the Professor Academic Development Foundation of Fujian Medical University of China,No.JS11003
文摘Neuro D plays a key regulatory effect on differentiation of neural stem cells into mature neurons in the brain.Thus,we assumed that electroacupuncture at Baihui（DU20） acupoint in newborn rats exposed to in utero fetal distress would influence expression of Neuro D.Electroacupuncture at Baihui was performed for 20 minutes on 3-day-old（Day 3） newborn Sprague-Dawley rats exposed to in utero fetal distress;electroacupuncture parameters consisted of sparse and dense waves at a frequency of 2–10 Hz.Real-time fluorescent quantitative PCR results demonstrated that m RNA expression of Neuro D,a molecule that indicates Neuro D,increased with prolonged time in brains of newborn rats,and peaked on Day 22.The level of m RNA expression was similar between Day 16 and Day 35.These findings suggest that electro acupuncture at Baihui acupoint could effectively increase m RNA expression of molecules involved in Neuro D in the brains of newborn rats exposed to in utero fetal distress.
文摘【目的】运用数据挖掘技术分析针刺治疗中风后失眠的选穴规律。【方法】通过检索中国期刊全文数据库(CNKI)、万方学术期刊全文数据库(Wanfang)、维普中文科技期刊数据库(VIP)、中国生物医学文献数据库(CBM),美国医学在线(PubMed)、核心合集数据库(Web of Science)以及荷兰医学文献数据库(Embase)等各大数据库,选取建库以来至2022年10月有关针刺治疗中风后失眠的临床研究文献,应用SPSS Statistic及SPSS Modeler构建数据库,采用关联规则分析及聚类分析,探究针刺治疗中风后失眠针刺处方选穴的规律。【结果】共纳入文献117篇,针刺处方119个,使用腧穴116种,腧穴总使用频次为887次。(1)穴位分析结果显示使用频次较高的腧穴为:百会、三阴交、内关、神庭、神门、四神聪。(2)选经以督脉、足太阳膀胱经、任脉为主,并且注重经外奇穴的使用。临床针刺部位主要选取头项、四肢部。(3)特定穴使用频次最高的为原穴、八脉交会穴和五输穴中的“输穴”。(4)关联规则分析显示,针刺治疗中风后失眠的核心腧穴组为百会-神庭-三阴交。(5)高频穴位聚类获得三个聚类群,第1类:百会;第2类:四神聪、内关、三阴交、神门、神庭;第3类:申脉、中脘、足三里、太冲、印堂、安眠、太溪、气海、关元、水沟。【结论】针刺治疗中风后失眠取穴方面多以督脉和头项部取穴为主;核心穴位为百会-神庭-三阴交;治则治法多遵循“引阳入阴,调整阴阳平衡”的原则。
