Objective To study the pharmacokinetic properties of Fufang Baijiezi Gel（FBG） after different acupoints administration. Methods Sinapine thiocyanate, tetrahydropalmatine, 6-gingerol, and asarinin, which were four su...Objective To study the pharmacokinetic properties of Fufang Baijiezi Gel（FBG） after different acupoints administration. Methods Sinapine thiocyanate, tetrahydropalmatine, 6-gingerol, and asarinin, which were four substances of FBG, were determined by a sensitive liquid chromatography tandem mass spectrometry method（LC-MS） both in plasma and dermal microdialysates of guinea pig simultaneously. Microdialysates were separated on an Ultimate？ XB-Phenyl analytical column（150 mm ×2.1 mm, 5μm） and detected by electrospray ionization（ESI） in selected ion monitoring（SIM） mode. The method was validated in terms of selectivity, linearity, sensitivity, and recovery. Result A significant difference was observed in main pharmacokinetic parameters of C max, t max, and AUC between acupoints administration and nonacupoints administration. Conclusion Acupoints administration resulted in a more obvious increase in bioavailability of sinapine thiocyanate, tetrahydropalmatine, 6-gingerol, and asarinin than nonacupoints administration.展开更多
目的:考察氮酮、丙二醇及二者混合物对白芥子散贴剂中延胡索乙素透过量的影响,为该复方的凝胶膏剂研制提供参考。方法:采用改良Franz扩散池,以离体雄性大白兔皮肤为模型,通过HPLC测定延胡索乙素含量,流动相甲醇-0.1%磷酸溶液(三乙胺调p ...目的:考察氮酮、丙二醇及二者混合物对白芥子散贴剂中延胡索乙素透过量的影响,为该复方的凝胶膏剂研制提供参考。方法:采用改良Franz扩散池,以离体雄性大白兔皮肤为模型,通过HPLC测定延胡索乙素含量,流动相甲醇-0.1%磷酸溶液(三乙胺调p H 6.0)(60∶40),检测波长280 nm,拟合延胡索乙素透皮吸收的累积透过量,考察不同促渗剂对白芥子散贴剂体外透皮效果的影响。结果:以2%氮酮制备的白芥子散贴剂累积透过量最高;促渗剂合用时以2%氮酮+5%丙二醇促渗作用较好,但其合用效果弱于2%氮酮单用的效果。结论:氮酮能显著提高白芥子散贴剂的透皮吸收效果。展开更多
基金National Natural Science Foundation of China(No.81001649)
文摘Objective To study the pharmacokinetic properties of Fufang Baijiezi Gel（FBG） after different acupoints administration. Methods Sinapine thiocyanate, tetrahydropalmatine, 6-gingerol, and asarinin, which were four substances of FBG, were determined by a sensitive liquid chromatography tandem mass spectrometry method（LC-MS） both in plasma and dermal microdialysates of guinea pig simultaneously. Microdialysates were separated on an Ultimate？ XB-Phenyl analytical column（150 mm ×2.1 mm, 5μm） and detected by electrospray ionization（ESI） in selected ion monitoring（SIM） mode. The method was validated in terms of selectivity, linearity, sensitivity, and recovery. Result A significant difference was observed in main pharmacokinetic parameters of C max, t max, and AUC between acupoints administration and nonacupoints administration. Conclusion Acupoints administration resulted in a more obvious increase in bioavailability of sinapine thiocyanate, tetrahydropalmatine, 6-gingerol, and asarinin than nonacupoints administration.
文摘目的:考察氮酮、丙二醇及二者混合物对白芥子散贴剂中延胡索乙素透过量的影响,为该复方的凝胶膏剂研制提供参考。方法:采用改良Franz扩散池,以离体雄性大白兔皮肤为模型,通过HPLC测定延胡索乙素含量,流动相甲醇-0.1%磷酸溶液(三乙胺调p H 6.0)(60∶40),检测波长280 nm,拟合延胡索乙素透皮吸收的累积透过量,考察不同促渗剂对白芥子散贴剂体外透皮效果的影响。结果:以2%氮酮制备的白芥子散贴剂累积透过量最高;促渗剂合用时以2%氮酮+5%丙二醇促渗作用较好,但其合用效果弱于2%氮酮单用的效果。结论:氮酮能显著提高白芥子散贴剂的透皮吸收效果。
