We present a theoretical study of the influence of a single silver sphere on the fluorescence of radix angelica dahurica, which is a kind of traditional Chinese medicine. The enhancement factors of the excitation and ...We present a theoretical study of the influence of a single silver sphere on the fluorescence of radix angelica dahurica, which is a kind of traditional Chinese medicine. The enhancement factors of the excitation and the relaxation processes are deduced. The excitation can be enhanced more than 100 times at 315 nm. The enhancement factor of the emission can reach up to 9 at a center wavelength of 400 nm.展开更多
Objective： To explore the direct and/or the enhancing antihypertensive effects of wind-dispelling herbs involvingFangfeng （Saposhnikoviae Radix） and Baizhi （Angelicae dahuricae Radix） using liver-yang hyperactivi...Objective： To explore the direct and/or the enhancing antihypertensive effects of wind-dispelling herbs involvingFangfeng （Saposhnikoviae Radix） and Baizhi （Angelicae dahuricae Radix） using liver-yang hyperactivity renalhypertension rat. Methods： Model rats with hyperactivity of liver-yang hyperactivity were prepared using male SD rats.Once successful, the rats were randomly divided into groups and given medicine by gavage for 4 weeks. Blood wascollected from the abdominal aorta to prepare serum. Serum nitric oxide （NO） concentration was determined bychemical colorimetry. The contents of ET, TXB2 and 6-K-PGF1a were determined by enzyme linked immunosorbentassay （ELISA）. Results： Compared with the control model group, the blood pressure, serum ET and TXB2 of rats in thewind dispelling medicine group was not significantly reduced, while those of rats in captopril group, Tianma GoutengDecoction I group （TGD group） decreased significantly （P 〈0.05 for both）. Interestingly, the blood pressure, serum ETand TXB2 of rats in the combination group （wind dispelling medicine ＆ TGD） decreased significantly compared withboth wind dispelling medicine group and TGD group. Meanwhile, the levels of serum NO and 6- K-PGF1a in thecombination group was much higher than those in the wind dispelling medicine group or TGD group （P 〈0.05 for both）.However, the levels of serum NO and 6- K-PGF1a in the wind dispelling medicine group was not significantly increasedcompared with the model group. Conclusion： There is no direct pressure lowering effects of wind dispelling medicinealone, while wind dispelling medicine could potentiates the antihypertensive effects of Tianma Gouteng Decoction I.Besides, the synergistic effects may be related to decreased ET and TXB2 levels and the increased NO and 6- K-PGF1alevels.展开更多
Objective:The objective was to study the potential substance basis and action mechanism of Chuanxiong Rhizoma(CX)and Angelicae Dahuricae Radix(AD)on analgesia through network pharmacology and molecular docking.Materia...Objective:The objective was to study the potential substance basis and action mechanism of Chuanxiong Rhizoma(CX)and Angelicae Dahuricae Radix(AD)on analgesia through network pharmacology and molecular docking.Materials and Methods:The active components and targets of CX and AD and pain-related genes were retrieved through Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)and GeneCards database.Then,the co-action targets were found,protein–protein interaction network was constructed by the String database.The Cytoscape 3.7.1 was used to construct"CX-AD-active components-pain"network.Further enrichment analysis of Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)was carried out to predict its mechanism of action,the top four active components in the network were docked with the targets.Results:There are 26 compounds,45 targets in the network.Among them,(Z)-ligustilide and beta-sitosterol,respectively,have more potential targets in CX and AD,and prostaglandin-endoperoxide synthase(PTGS2),PTGS1 have more ligands.GO analysis shows that molecular functions of CX and AD mainly performed through the G protein-coupled amine receptor activity,adrenergic receptor activity,and catecholamine binding.KEGG analysis indicates that they could exert analgesic effect on the pathways of regulating neuroactive ligand-receptor interaction,serotonergic synapse,and cGMP-PKG signaling pathway.Molecular docking results show that the active compounds are highly compatible with the structure of the protein receptor,and they interact through the hydrogen bond andπ–πbond between the ligand and the active site residues.Conclusions:Through network pharmacology and molecular docking,this study preliminarily revealed the main active components,targets,and potential regulation network of CX and AD,providing a reference for the subsequent experimental research.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.61405085 and 61275147)the Research Fund for the Doctoral Program of Liaocheng University,China+1 种基金the Key Project of Science and Technology of Shandong Province of China(Grant No.2010GGX10127)the Shandong Province Natural Science Foundation of China(Grant Nos.ZR2013EML006 and ZR2012AL11)
文摘We present a theoretical study of the influence of a single silver sphere on the fluorescence of radix angelica dahurica, which is a kind of traditional Chinese medicine. The enhancement factors of the excitation and the relaxation processes are deduced. The excitation can be enhanced more than 100 times at 315 nm. The enhancement factor of the emission can reach up to 9 at a center wavelength of 400 nm.
文摘Objective： To explore the direct and/or the enhancing antihypertensive effects of wind-dispelling herbs involvingFangfeng （Saposhnikoviae Radix） and Baizhi （Angelicae dahuricae Radix） using liver-yang hyperactivity renalhypertension rat. Methods： Model rats with hyperactivity of liver-yang hyperactivity were prepared using male SD rats.Once successful, the rats were randomly divided into groups and given medicine by gavage for 4 weeks. Blood wascollected from the abdominal aorta to prepare serum. Serum nitric oxide （NO） concentration was determined bychemical colorimetry. The contents of ET, TXB2 and 6-K-PGF1a were determined by enzyme linked immunosorbentassay （ELISA）. Results： Compared with the control model group, the blood pressure, serum ET and TXB2 of rats in thewind dispelling medicine group was not significantly reduced, while those of rats in captopril group, Tianma GoutengDecoction I group （TGD group） decreased significantly （P 〈0.05 for both）. Interestingly, the blood pressure, serum ETand TXB2 of rats in the combination group （wind dispelling medicine ＆ TGD） decreased significantly compared withboth wind dispelling medicine group and TGD group. Meanwhile, the levels of serum NO and 6- K-PGF1a in thecombination group was much higher than those in the wind dispelling medicine group or TGD group （P 〈0.05 for both）.However, the levels of serum NO and 6- K-PGF1a in the wind dispelling medicine group was not significantly increasedcompared with the model group. Conclusion： There is no direct pressure lowering effects of wind dispelling medicinealone, while wind dispelling medicine could potentiates the antihypertensive effects of Tianma Gouteng Decoction I.Besides, the synergistic effects may be related to decreased ET and TXB2 levels and the increased NO and 6- K-PGF1alevels.
基金financially supported by NSFC-DFG(No.81861138042)Natural Science Foundation of China(No.81673634)+1 种基金Natural Science Foundation of Shandong,China(No.ZR2019MC004)the high-end talent team construction foundation(No.108-10000318)
文摘Objective:The objective was to study the potential substance basis and action mechanism of Chuanxiong Rhizoma(CX)and Angelicae Dahuricae Radix(AD)on analgesia through network pharmacology and molecular docking.Materials and Methods:The active components and targets of CX and AD and pain-related genes were retrieved through Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)and GeneCards database.Then,the co-action targets were found,protein–protein interaction network was constructed by the String database.The Cytoscape 3.7.1 was used to construct"CX-AD-active components-pain"network.Further enrichment analysis of Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)was carried out to predict its mechanism of action,the top four active components in the network were docked with the targets.Results:There are 26 compounds,45 targets in the network.Among them,(Z)-ligustilide and beta-sitosterol,respectively,have more potential targets in CX and AD,and prostaglandin-endoperoxide synthase(PTGS2),PTGS1 have more ligands.GO analysis shows that molecular functions of CX and AD mainly performed through the G protein-coupled amine receptor activity,adrenergic receptor activity,and catecholamine binding.KEGG analysis indicates that they could exert analgesic effect on the pathways of regulating neuroactive ligand-receptor interaction,serotonergic synapse,and cGMP-PKG signaling pathway.Molecular docking results show that the active compounds are highly compatible with the structure of the protein receptor,and they interact through the hydrogen bond andπ–πbond between the ligand and the active site residues.Conclusions:Through network pharmacology and molecular docking,this study preliminarily revealed the main active components,targets,and potential regulation network of CX and AD,providing a reference for the subsequent experimental research.
