From January 2001 to January 2002, 50 patients of candidal vaginitis were treated by Kushen powder (KSP,苦参散) combined with Canesten as the treatment group, and controlled by a control group including another 50 pat...From January 2001 to January 2002, 50 patients of candidal vaginitis were treated by Kushen powder (KSP,苦参散) combined with Canesten as the treatment group, and controlled by a control group including another 50 patients treated with Canesten alone. The therapeutic results were satisfactory and reported as follows.展开更多
Heavy metal contamination to the environment is a serious problem in the developing countries due to anthropogenic activities, a threat needs to remediate to sustain the life on earth, technology includes use of micro...Heavy metal contamination to the environment is a serious problem in the developing countries due to anthropogenic activities, a threat needs to remediate to sustain the life on earth, technology includes use of microorganisms and different plant species. In consideration of biomass, phytoremediation is a very useful techniques above all, can be exploit by identification of hyperaccumulator, which accumulates the heavy metal under metal stress condition. In view of constraints of efficient plant species in present study thirty seven different plant species were screened for the identification of heavy metal accumulators. Croton bonplandianum, sedges and Balanites aegyptiaca amongst the all exhibit superior potential of heavy metal accumulation. This is the first report to unravel the heavy metal accumulation property of three different plant species which can be exploited for the bioremediation of heavy metals.展开更多
Background: Penile toxicity after preoperative concurrent chemoradiation (CCRT) for rectal cancers is extremely rare and only two cases of phimosis and one case of recto-cavernosalfistula have been reported so far in ...Background: Penile toxicity after preoperative concurrent chemoradiation (CCRT) for rectal cancers is extremely rare and only two cases of phimosis and one case of recto-cavernosalfistula have been reported so far in literature. Preoperative CCRT for rectal cancer is given in prone position and with the support of belly board (BBD) to avoid small bowel toxicity. However, positional errors during rectal radiotherapy can lead to unexpected penile toxicity. Case Presentation: A 50-year-old Saudi male with diagnosed case of rectal adenocarcinoma stage cT3N1M0 was given preoperative CCRT 50.4 Gy in 28 fractions with three-dimensional conformal radiation therapy (3DCRT) in prone position using belly board with concurrent oral capecitabine 825 mg/2 twice a day. After the completion of CCRT, he complained of severe soreness, itching over glans penis and dysuria. Examination revealed grade 3 erythema, skin desquamation over glans penis (balanitis). Portal imaging of treatment revealed glans penis to lie within posterior radiation beam. A patient was assured and he recovered fully after local steroids and short course of antibiotics. Conclusion: Penile toxicity after CCRT for rectal cancer is extremely rare manifestation. Radiation oncologists and therapists must be aware of this rare side effect and must assure proper patient education and positioning during CCRT for rectal cancer.展开更多
Mostly candida resides as an opportunistic organism on epithelial surfaces of human being. However, under auspicious conditions can cause infections including serious life threatening invasive candidiasis with subsequ...Mostly candida resides as an opportunistic organism on epithelial surfaces of human being. However, under auspicious conditions can cause infections including serious life threatening invasive candidiasis with subsequent mortality particularly in immune deficit and hospitalized patients having co-morbids. Limited data are published on the prevalence of candidiasis, based on the researches conducted at few tertiary care settings which are not representing the overall disease burden in our country, Pakistan. Therefore, this study was conducted to evaluate the frequency and sensitivity patterns of candidiasis in our community. Methods: Out of total 1020 specimens, 130 clinical samples were identified as candidal positive, obtained from March to May 2018. These samples were isolated from vagina, oropharynx, urine, tracheal aspirates, pus, blood, tips of the intubations, wounds and fluids of the body cavities. Identification of candida, its species and antifungal sensitivity screening was done by Kirby Bauer’s disk diffusion method according to CLSI guide lines’ (M - 44 A2 series, 2009). Results: A significant majority, 80 (61.5%) of candidal strains were isolated from females with female to male ratio 8:5 and most of these isolates were obtained from high vaginal swabs (43.75%). Four candidal species (Candida albicans 80%, Candida tropicalis 10%, Candida glabrata 9.2% and Candida ciferrii 0.8%) were isolated from all positive specimens. Maximum number of the positive samples 52 (40%) were obtained from ICU patients. Sensitivity test of candidal positive samples revealed that commonly used azole antifungal drugs, fluconazole and voriconazole were highly resistant, with respective 57.7% and 70.8% resistance. Conclusion: Candidiasis is highly prevalent in our clinical set up and more frequently infecting females in comparison to males as most of the positive isolates were retrieved from HVS (high vaginal swabs). Still, C. albicans was found to be the most prevalent specie isolated among all candida samples. Our study also demonstrated that the resistance of most commonly prescribed antifungals, azoles have shown a rapid rise. Therefore, it is recommended that before prescription of antifungal drugs the clinicians should routinely recommend culture and sensitivity testing of samples taken from candida infected individuals.展开更多
维生素E(Ve)是大豆油中一种天然抗氧化剂,是评价大豆油营养价值的重要指标。本研究利用含有264份的大豆自然群体在2021年和2022年测定了籽粒中α-、γ-和δ-生育酚含量,并进行全基因组关联分析(Genome-wide association study,GWAS)。...维生素E(Ve)是大豆油中一种天然抗氧化剂,是评价大豆油营养价值的重要指标。本研究利用含有264份的大豆自然群体在2021年和2022年测定了籽粒中α-、γ-和δ-生育酚含量,并进行全基因组关联分析(Genome-wide association study,GWAS)。本研究共检测到199个与大豆Ve含量显著关联的SNP位点,其中9个可在2个环境或者2个性状被重复检测到,分别位于3号、7号、11号、12号、13号、15号、17号和18号染色体上。其中位于7号染色体上的显著关联信号是控制α-生育酚含量的主效位点,可在2年环境中被检测到,表型变异解释率为9.83%。对该位点候选基因进行筛选,获得一个编码myb转录因子的基因Glyma.07G054000,可能是这个位点的效应基因。另外,在12号染色体上得到2个编码γ-生育酚甲基转移酶的基因Glyma.12G014200和Glyma.12G014300,有可能是影响Ve含量的重要基因。本研究结果有助于解析大豆籽粒Ve含量的遗传基础及其调控机制,为大豆品质遗传改良奠定了基础。展开更多
谷子(Setaria italica L.)是我国北方地区重要的粮食作物,籽粒营养丰富,且富含多种类黄酮物质,对生长发育和品质形成发挥着重要作用。目前谷子籽粒类黄酮合成及粒色形成相关调控机制研究较少。分析谷子类黄酮含量及粒色性状相关的QTL,...谷子(Setaria italica L.)是我国北方地区重要的粮食作物,籽粒营养丰富,且富含多种类黄酮物质,对生长发育和品质形成发挥着重要作用。目前谷子籽粒类黄酮合成及粒色形成相关调控机制研究较少。分析谷子类黄酮含量及粒色性状相关的QTL,为类黄酮合成关键基因的精细定位、克隆及功能研究奠定基础,同时,也为揭示谷子类黄酮合成及代谢机制和培育富含类黄酮谷子品种提供技术支撑。本研究以红粒色高类黄酮品种金苗红酒谷和黄粒色低类黄酮品种豫谷28为亲本构建的包含150个家系的重组自交系(RIL)群体为试验材料,在谷子成熟期对籽粒粒色和类黄酮含量相关性状进行分析。同时,采用复合区间作图法(composite interval mapping,CIM)对粒色和类黄酮含量进行QTL定位与分析,并对QTL置信区间内的候选基因进行预测。相关性分析表明,类黄酮含量与粒色呈显著正相关。共定位到4个与类黄酮含量相关和11个与粒色相关的QTL,分别位于1号、2号、5号、6号、7号、8号和9号染色体上,单个QTL的表型贡献率为2.01%~29.25%,6个为主效QTL,其中,qSC1-2和qFLA1-1、qSC7-1和qFLA7-1、qSC9-3和qFLA9-1为2个性状下共同定位到的QTL。通过基因预测与功能注释,筛选出QTL置信区间内5个与类黄酮物质合成及代谢相关的候选基因,表明类黄酮物质的合成、代谢及利用相关基因极有可能控制了这些基因的表达。15个QTL分别聚集于7条染色体上,基于基因功能注释,共筛选了5个与谷子类黄酮合成及代谢相关的候选基因,表明不同QTL位点参与到了共同遗传机制,并可通过分子标记辅助选择进行类黄酮合成及代谢等有利基因的聚合育种。展开更多
玉米籽粒氮含量与品质紧密相关,其遗传机制的解析对玉米品质育种具有重要意义。本研究以252份玉米自交系为关联群体,利用贝叶斯信息与连锁不平衡迭代嵌套式模型(BLINK,bayesian-informationandlinkage-disequilibrium iteratively neste...玉米籽粒氮含量与品质紧密相关,其遗传机制的解析对玉米品质育种具有重要意义。本研究以252份玉米自交系为关联群体,利用贝叶斯信息与连锁不平衡迭代嵌套式模型(BLINK,bayesian-informationandlinkage-disequilibrium iteratively nested keyway)、固定随机循环概率模型(FarmCPU,fixed and random model circulating probability unification)、一般线性模型(GLM,general linear model)、混合线性模型(MLM,mixed linear model)、多位点混合线性模型(MLMM,multiple loci mixed model)和逐步排它性混合线性模型(SUPER,settlement of MLM under progressively exclusive relationship)等方法分别对其籽粒氮含量进行全基因组关联分析。共鉴定到13个与籽粒氮含量显著关联的SNP(P<3.64E-07)。BLINK、FarmCPU、GLM、MLM、MLMM和SUPER方法分别检测到6个、3个、7个、4个、2个和4个SNP位点。其中,S3_8879213在5种方法中均能检测到,S9_146173702在4种方法中均能检测到,S5_114774030和S7_182217338在3种方法中均能检测到,S1_10906326和S1_177528813在2种方法中均能检测到。共挖掘25个相关候选基因,其中Zm00001eb275080和Zm00001eb330700可能是影响玉米籽粒氮含量的重要候选基因。展开更多
文摘From January 2001 to January 2002, 50 patients of candidal vaginitis were treated by Kushen powder (KSP,苦参散) combined with Canesten as the treatment group, and controlled by a control group including another 50 patients treated with Canesten alone. The therapeutic results were satisfactory and reported as follows.
文摘Heavy metal contamination to the environment is a serious problem in the developing countries due to anthropogenic activities, a threat needs to remediate to sustain the life on earth, technology includes use of microorganisms and different plant species. In consideration of biomass, phytoremediation is a very useful techniques above all, can be exploit by identification of hyperaccumulator, which accumulates the heavy metal under metal stress condition. In view of constraints of efficient plant species in present study thirty seven different plant species were screened for the identification of heavy metal accumulators. Croton bonplandianum, sedges and Balanites aegyptiaca amongst the all exhibit superior potential of heavy metal accumulation. This is the first report to unravel the heavy metal accumulation property of three different plant species which can be exploited for the bioremediation of heavy metals.
文摘Background: Penile toxicity after preoperative concurrent chemoradiation (CCRT) for rectal cancers is extremely rare and only two cases of phimosis and one case of recto-cavernosalfistula have been reported so far in literature. Preoperative CCRT for rectal cancer is given in prone position and with the support of belly board (BBD) to avoid small bowel toxicity. However, positional errors during rectal radiotherapy can lead to unexpected penile toxicity. Case Presentation: A 50-year-old Saudi male with diagnosed case of rectal adenocarcinoma stage cT3N1M0 was given preoperative CCRT 50.4 Gy in 28 fractions with three-dimensional conformal radiation therapy (3DCRT) in prone position using belly board with concurrent oral capecitabine 825 mg/2 twice a day. After the completion of CCRT, he complained of severe soreness, itching over glans penis and dysuria. Examination revealed grade 3 erythema, skin desquamation over glans penis (balanitis). Portal imaging of treatment revealed glans penis to lie within posterior radiation beam. A patient was assured and he recovered fully after local steroids and short course of antibiotics. Conclusion: Penile toxicity after CCRT for rectal cancer is extremely rare manifestation. Radiation oncologists and therapists must be aware of this rare side effect and must assure proper patient education and positioning during CCRT for rectal cancer.
文摘Mostly candida resides as an opportunistic organism on epithelial surfaces of human being. However, under auspicious conditions can cause infections including serious life threatening invasive candidiasis with subsequent mortality particularly in immune deficit and hospitalized patients having co-morbids. Limited data are published on the prevalence of candidiasis, based on the researches conducted at few tertiary care settings which are not representing the overall disease burden in our country, Pakistan. Therefore, this study was conducted to evaluate the frequency and sensitivity patterns of candidiasis in our community. Methods: Out of total 1020 specimens, 130 clinical samples were identified as candidal positive, obtained from March to May 2018. These samples were isolated from vagina, oropharynx, urine, tracheal aspirates, pus, blood, tips of the intubations, wounds and fluids of the body cavities. Identification of candida, its species and antifungal sensitivity screening was done by Kirby Bauer’s disk diffusion method according to CLSI guide lines’ (M - 44 A2 series, 2009). Results: A significant majority, 80 (61.5%) of candidal strains were isolated from females with female to male ratio 8:5 and most of these isolates were obtained from high vaginal swabs (43.75%). Four candidal species (Candida albicans 80%, Candida tropicalis 10%, Candida glabrata 9.2% and Candida ciferrii 0.8%) were isolated from all positive specimens. Maximum number of the positive samples 52 (40%) were obtained from ICU patients. Sensitivity test of candidal positive samples revealed that commonly used azole antifungal drugs, fluconazole and voriconazole were highly resistant, with respective 57.7% and 70.8% resistance. Conclusion: Candidiasis is highly prevalent in our clinical set up and more frequently infecting females in comparison to males as most of the positive isolates were retrieved from HVS (high vaginal swabs). Still, C. albicans was found to be the most prevalent specie isolated among all candida samples. Our study also demonstrated that the resistance of most commonly prescribed antifungals, azoles have shown a rapid rise. Therefore, it is recommended that before prescription of antifungal drugs the clinicians should routinely recommend culture and sensitivity testing of samples taken from candida infected individuals.
文摘维生素E(Ve)是大豆油中一种天然抗氧化剂,是评价大豆油营养价值的重要指标。本研究利用含有264份的大豆自然群体在2021年和2022年测定了籽粒中α-、γ-和δ-生育酚含量,并进行全基因组关联分析(Genome-wide association study,GWAS)。本研究共检测到199个与大豆Ve含量显著关联的SNP位点,其中9个可在2个环境或者2个性状被重复检测到,分别位于3号、7号、11号、12号、13号、15号、17号和18号染色体上。其中位于7号染色体上的显著关联信号是控制α-生育酚含量的主效位点,可在2年环境中被检测到,表型变异解释率为9.83%。对该位点候选基因进行筛选,获得一个编码myb转录因子的基因Glyma.07G054000,可能是这个位点的效应基因。另外,在12号染色体上得到2个编码γ-生育酚甲基转移酶的基因Glyma.12G014200和Glyma.12G014300,有可能是影响Ve含量的重要基因。本研究结果有助于解析大豆籽粒Ve含量的遗传基础及其调控机制,为大豆品质遗传改良奠定了基础。
文摘谷子(Setaria italica L.)是我国北方地区重要的粮食作物,籽粒营养丰富,且富含多种类黄酮物质,对生长发育和品质形成发挥着重要作用。目前谷子籽粒类黄酮合成及粒色形成相关调控机制研究较少。分析谷子类黄酮含量及粒色性状相关的QTL,为类黄酮合成关键基因的精细定位、克隆及功能研究奠定基础,同时,也为揭示谷子类黄酮合成及代谢机制和培育富含类黄酮谷子品种提供技术支撑。本研究以红粒色高类黄酮品种金苗红酒谷和黄粒色低类黄酮品种豫谷28为亲本构建的包含150个家系的重组自交系(RIL)群体为试验材料,在谷子成熟期对籽粒粒色和类黄酮含量相关性状进行分析。同时,采用复合区间作图法(composite interval mapping,CIM)对粒色和类黄酮含量进行QTL定位与分析,并对QTL置信区间内的候选基因进行预测。相关性分析表明,类黄酮含量与粒色呈显著正相关。共定位到4个与类黄酮含量相关和11个与粒色相关的QTL,分别位于1号、2号、5号、6号、7号、8号和9号染色体上,单个QTL的表型贡献率为2.01%~29.25%,6个为主效QTL,其中,qSC1-2和qFLA1-1、qSC7-1和qFLA7-1、qSC9-3和qFLA9-1为2个性状下共同定位到的QTL。通过基因预测与功能注释,筛选出QTL置信区间内5个与类黄酮物质合成及代谢相关的候选基因,表明类黄酮物质的合成、代谢及利用相关基因极有可能控制了这些基因的表达。15个QTL分别聚集于7条染色体上,基于基因功能注释,共筛选了5个与谷子类黄酮合成及代谢相关的候选基因,表明不同QTL位点参与到了共同遗传机制,并可通过分子标记辅助选择进行类黄酮合成及代谢等有利基因的聚合育种。
文摘玉米籽粒氮含量与品质紧密相关,其遗传机制的解析对玉米品质育种具有重要意义。本研究以252份玉米自交系为关联群体,利用贝叶斯信息与连锁不平衡迭代嵌套式模型(BLINK,bayesian-informationandlinkage-disequilibrium iteratively nested keyway)、固定随机循环概率模型(FarmCPU,fixed and random model circulating probability unification)、一般线性模型(GLM,general linear model)、混合线性模型(MLM,mixed linear model)、多位点混合线性模型(MLMM,multiple loci mixed model)和逐步排它性混合线性模型(SUPER,settlement of MLM under progressively exclusive relationship)等方法分别对其籽粒氮含量进行全基因组关联分析。共鉴定到13个与籽粒氮含量显著关联的SNP(P<3.64E-07)。BLINK、FarmCPU、GLM、MLM、MLMM和SUPER方法分别检测到6个、3个、7个、4个、2个和4个SNP位点。其中,S3_8879213在5种方法中均能检测到,S9_146173702在4种方法中均能检测到,S5_114774030和S7_182217338在3种方法中均能检测到,S1_10906326和S1_177528813在2种方法中均能检测到。共挖掘25个相关候选基因,其中Zm00001eb275080和Zm00001eb330700可能是影响玉米籽粒氮含量的重要候选基因。
