The objective of this work was to investigate the mechanism of action of Balanophora involucrata polyphenolic compounds in the treatment of myocardial injury.In the present study,Balanophora involucrata was extracted ...The objective of this work was to investigate the mechanism of action of Balanophora involucrata polyphenolic compounds in the treatment of myocardial injury.In the present study,Balanophora involucrata was extracted by refluxing 75%of ethanol.The obtained extract was extracted with petroleum ether,ethyl acetate and n-butanol respectively.And the ethyl acetate layer was separated.The extract was prepared by silica gel column chromatography,sephadex LH-20 elution and thin layer chromatography.After that,the Swiss target prediction database was utilized to obtain the targets of Balanophora involucrata,and the Genecards,OMIM and TTD databases were used to predict and screen the targets of Balanophora involucrata for the treatment of myocardial injury.The active ingredient-target network was constructed using Cytoscape software,and the PPI network was mapped using String database and Cytoscape software.GO bioprocess enrichment analysis and KEGG pathway enrichment analysis were performed by Metascape software to predict the mechanism of action.Molecular docking was performed in Discovery Studio 2016 client software to verify the binding of Balanophora involucrata polyphenols to key targets.In this study,six polyphenolic compounds were isolated from Balanophora involucrata.By GO enrichment analysis,1614 biological processes(BP),127 cellular compositions(CC),and 215 molecular functions(MF)were obtained;a total of 155 cross-targets were involved in the KEGG enrichment analysis.The PPI network showed that quercetin was the main active component of polyphenolic compounds against myocardial injury and that AKT1,EGFR,STAT3,SRC,ESR1,MMP9,HSP90AA1 and other related signals were associated with myocardial injury treatment.Finally,the multi-component-multi-target-multi-pathway action of Balanophora involucrata was concluded,which provided new ideas and methods for further research on the mechanism of action of Balanophora involucrata in myocardial injury.展开更多
Holoparasitism is a special life cycle of flowering plants. All carbon resources are provided by photosynthetic host plants. A recent study revealed the presence of endophytic fungi in holoparasitic plants, but their ...Holoparasitism is a special life cycle of flowering plants. All carbon resources are provided by photosynthetic host plants. A recent study revealed the presence of endophytic fungi in holoparasitic plants, but their ecological and evolutionary roles are still unknown. In this study, we examined endophytic fungi isolated from the holoparasitic plant Balanophora japonica (Balanophoraceae), collected from Kochi, Shikoku in western Japan. We isolated 23 fungal strains on inflorescences and tubers from three B. japonica plants at two locations and on one sample of the host plant (Symplocos lancifolia, Symplocaceae). Predominant isolates were Trichoderma-Hypocrea, Penicillium and Phialemonium. The first group was also predominant in the host plant. Fungal composition revealed in this study differed from the composition on B. harlandii or other root holoparasites with endophytic fungal (Rafflesia cantleyi) data. Those differences might be caused by various factors, including growth habits, location, phylogenetic position or host-parasite relationship.展开更多
This study reports an investigation of the chemical constituents of Balanophora polyandra Griff.Fifteen compounds were isolated by column chromatography on silica gel,Toyo-pearl HW-40C,Sephadex LH-20 and by HPLC.Their...This study reports an investigation of the chemical constituents of Balanophora polyandra Griff.Fifteen compounds were isolated by column chromatography on silica gel,Toyo-pearl HW-40C,Sephadex LH-20 and by HPLC.Their structures were elucidated as 1,4-di-O galloy-2-O-[(E)-p-coumaroyl]-β-D-glucopyranose(1),1-O-galloyl-β-D-pyranglucose(2),1-p-coumaryl-β-D-pyranglu-cose(3),1-O-(E)-caffeoyl-β-D-pyranglucose(4),1,3-di-O-galloyl-β-D-pyranglucose(5),1,6-di-O-galloyl-β-D-pyranglucose(6),1-O-(E)-caffeoyl-4-O-galloyl-β-D-pyranglucose(7),1-O-(E)-caffeoy1-6-O galloyl-β-D-pyranglucose(8),1-0-(E)-caffeoy1-4,6-di-O-galloyl-β-D-pyranglucose(9),1-O-(E)-caffeoyl-4,6-(S)-HHDP-B-D-pyranglucose(10),1,23,6-tetra-O-galloyl-β-D-pyranglucose(11),4,6-(S)-hexahydroxydi-phenoyl-(α/β)-D-glucose(12),1-O-(E)-caffeoyl-4,6-[1′,1″-3′,3″,4′,4″-tetrahydroxydibenzofurandicar-boxyl)]-β-D glucopyranose(13),flavogallonic acid(14),and phloretin-4′-O-β-D-glucoside(15)on the basis of spectral analysis.Compound 1 was a new hydrolyzable tannin,9 was obtained from this genus for the first time,and compounds 5,6 and 11-14 were isolated from this plant for the first time.展开更多
基金Project supported by National Training Program of Innovation and Entrepreneurship for Undergraduates(202310163020,S202310163079).
文摘The objective of this work was to investigate the mechanism of action of Balanophora involucrata polyphenolic compounds in the treatment of myocardial injury.In the present study,Balanophora involucrata was extracted by refluxing 75%of ethanol.The obtained extract was extracted with petroleum ether,ethyl acetate and n-butanol respectively.And the ethyl acetate layer was separated.The extract was prepared by silica gel column chromatography,sephadex LH-20 elution and thin layer chromatography.After that,the Swiss target prediction database was utilized to obtain the targets of Balanophora involucrata,and the Genecards,OMIM and TTD databases were used to predict and screen the targets of Balanophora involucrata for the treatment of myocardial injury.The active ingredient-target network was constructed using Cytoscape software,and the PPI network was mapped using String database and Cytoscape software.GO bioprocess enrichment analysis and KEGG pathway enrichment analysis were performed by Metascape software to predict the mechanism of action.Molecular docking was performed in Discovery Studio 2016 client software to verify the binding of Balanophora involucrata polyphenols to key targets.In this study,six polyphenolic compounds were isolated from Balanophora involucrata.By GO enrichment analysis,1614 biological processes(BP),127 cellular compositions(CC),and 215 molecular functions(MF)were obtained;a total of 155 cross-targets were involved in the KEGG enrichment analysis.The PPI network showed that quercetin was the main active component of polyphenolic compounds against myocardial injury and that AKT1,EGFR,STAT3,SRC,ESR1,MMP9,HSP90AA1 and other related signals were associated with myocardial injury treatment.Finally,the multi-component-multi-target-multi-pathway action of Balanophora involucrata was concluded,which provided new ideas and methods for further research on the mechanism of action of Balanophora involucrata in myocardial injury.
文摘Holoparasitism is a special life cycle of flowering plants. All carbon resources are provided by photosynthetic host plants. A recent study revealed the presence of endophytic fungi in holoparasitic plants, but their ecological and evolutionary roles are still unknown. In this study, we examined endophytic fungi isolated from the holoparasitic plant Balanophora japonica (Balanophoraceae), collected from Kochi, Shikoku in western Japan. We isolated 23 fungal strains on inflorescences and tubers from three B. japonica plants at two locations and on one sample of the host plant (Symplocos lancifolia, Symplocaceae). Predominant isolates were Trichoderma-Hypocrea, Penicillium and Phialemonium. The first group was also predominant in the host plant. Fungal composition revealed in this study differed from the composition on B. harlandii or other root holoparasites with endophytic fungal (Rafflesia cantleyi) data. Those differences might be caused by various factors, including growth habits, location, phylogenetic position or host-parasite relationship.
基金This research program was supported by the National Natural Science Foundation of China(Grant No.30772709).
文摘This study reports an investigation of the chemical constituents of Balanophora polyandra Griff.Fifteen compounds were isolated by column chromatography on silica gel,Toyo-pearl HW-40C,Sephadex LH-20 and by HPLC.Their structures were elucidated as 1,4-di-O galloy-2-O-[(E)-p-coumaroyl]-β-D-glucopyranose(1),1-O-galloyl-β-D-pyranglucose(2),1-p-coumaryl-β-D-pyranglu-cose(3),1-O-(E)-caffeoyl-β-D-pyranglucose(4),1,3-di-O-galloyl-β-D-pyranglucose(5),1,6-di-O-galloyl-β-D-pyranglucose(6),1-O-(E)-caffeoyl-4-O-galloyl-β-D-pyranglucose(7),1-O-(E)-caffeoy1-6-O galloyl-β-D-pyranglucose(8),1-0-(E)-caffeoy1-4,6-di-O-galloyl-β-D-pyranglucose(9),1-O-(E)-caffeoyl-4,6-(S)-HHDP-B-D-pyranglucose(10),1,23,6-tetra-O-galloyl-β-D-pyranglucose(11),4,6-(S)-hexahydroxydi-phenoyl-(α/β)-D-glucose(12),1-O-(E)-caffeoyl-4,6-[1′,1″-3′,3″,4′,4″-tetrahydroxydibenzofurandicar-boxyl)]-β-D glucopyranose(13),flavogallonic acid(14),and phloretin-4′-O-β-D-glucoside(15)on the basis of spectral analysis.Compound 1 was a new hydrolyzable tannin,9 was obtained from this genus for the first time,and compounds 5,6 and 11-14 were isolated from this plant for the first time.
