In vivo and in vitro phytochemical and antibacterial efficacy of Baliospermum montanum (Willd.) Muell.Arg.

Objective:To evaluate the phytochemical and anti-bacterial potential of mother plants in vivo and in vitro derived callus of Baliospermum montanum(B.montanum)(Willd.) Muell.-Arg. leaves and root.Methods:The in vitro derived rootlets and leaves segments of B.montanum were cut into 0.5-0.7 cm in length and cultured on Murashige and Skoog solid medium supplemented with 3%sucrose,gelled with 0.7%agar and different concentration of 2,4-D either alone or in combinations.The preliminary phytochemical screening was performed by Harborne method.Antibacterial efficacy was performed by well diffusion method and incubated for 24 h at 37℃.Results:The highest percentage of callus formation(leaves segments 86.9±0.56:root segments 78.7±0.51) was obtained on Murashige and Skoog’s basal medium supplemented with 3%sucrose and 2.0 mg/L of 2.4-Dichlorophenoxy acetic acid.The phytochemical study revealed the high quantity presence of steroids,triterpenoids,glycosides,saponins,alkaloids,flavanoids, phenolic compounds,tannins,sugars etc of root and leaves derived calli.The ethanol extract of leaves segment derived calli of B.montanum showed the maximum solubility and antimicrobial activity with the MIC ranged from 100 to 200μL.Conclusions:The preliminary phytochemical study confirmed that the calli mediated tissues showed the higher percentage of metabolite constituents and extraction value compared to the in vivo leaves and roots.The present study observation suggested that a possibility to establish high yielding genotypes by in vitro culture for production of medicinally important bioactive compounds.

斑籽枝叶的化学成分研究

目的:研究斑籽枝叶的化学成分。方法:采用正相硅胶、MCI、ODS、RP-HPLC等色谱技术进行分离纯化,综合运用MS、1H-NMR及13C-NMR等波谱学方法对化合物进行结构鉴定。结果:从斑籽枝叶中分离得到9个化合物,分别鉴定为:6-hydroxy-cembrene-5,10-dione(1)、12-hydroxy-13-methylpodocarpa-9,11,13-trien-3-one(2)、白术内酯Ⅱ(3)、白术内酯Ⅲ(4)、α-tocopherol quinone(5)、α-tocospiro B(6)、4-hydroxy-2,3-dimethyl-2-nonen-4-olide(7)、5,7,4′-三甲氧基黄烷-3-醇(8)、1-(3,4-二甲氧基苯基)-1-丙酮(9)。结论:其中,化合物1~8为首次从该属植物中分离得到。