Balsam fir is an important Christmas tree species, especially in eastern Canada. The natural Christmas tree industry faces a challenge in postharvest needle abscission. Though there have been many studies describing t...Balsam fir is an important Christmas tree species, especially in eastern Canada. The natural Christmas tree industry faces a challenge in postharvest needle abscission. Though there have been many studies describing the physiological triggers and consequences in postharvest balsam fir, there have been no studies describing morphological or ultrastructural changes. Therefore, the objective of this study was to examine changes in stomata and chloroplast of postharvest needles. Branches were collected from low and high needle abscission resistance balsam fir genotypes, placed in water, and displayed in typical household conditions for 11 weeks. Needle abscission, chlorophyll fluorescence, and water uptake were monitored throughout. Needles stomata and chloroplasts were examined under a scanning and transmission electron microscope, respectively, each week. All branches had increased abscission, decreased chlorophyll fluorescence, and decreased water uptake over time. Needle surfaces accumulated fungal hyphae, especially in stomata. Chloroplasts demonstrated some dysfunction within two weeks, with notable decreases in chloroplast starch and increases in plastoglobulins. Within several weeks thylakoid membranes had been dismantled as chloroplasts transformed into gerontoplasts. All biophysical and structural changes were more pronounced in low needle abscission resistant genotypes. This research identifies a potential role for needle fungi in postharvest needle abscission and confirms the postharvest senescence of chloroplasts. Though it was previously speculated that chloroplasts must senesce postharvest, this study identifies how quickly this process occurs and that it occurs at different rates in contrasting genotypes.展开更多
The history of the Black Mountains in North Carolina and the southern Spruce-Fir ecosystem has been fraught with widespread forest decline since the mid 1960’s. Balsam Woolly Adelgid attacks and acidic deposition wer...The history of the Black Mountains in North Carolina and the southern Spruce-Fir ecosystem has been fraught with widespread forest decline since the mid 1960’s. Balsam Woolly Adelgid attacks and acidic deposition were two of the most recognized causes of decline. Uncertainty arose about the future of these forests, and projections were made regarding the endangerment or extinction of the endemic Fraser fir ([Pursh] Poiret). This study analyzed data sets from a permanent plot network in the Black Mountains dating 1985, 2002, and 2012. Indications that the Fraser fir population is stabilizing from a “boom-bust” cycle of population growth and has entered the stem exclusion stage of forest stand development are evident. Fir live stem density increased more than 250% from 1985 to 2002, and then declined 40% by 2012 at the highest elevations in the forest. Overall, fir appeared to be more impacted on western facing slopes than eastern ones. The population of red spruce experienced a steady decrease in live stem counts, but an increase in live basal area through all years, and at all elevation classes (1675 m, 1830 m, and 1980 m), indicating a normal progression through stand development. Red spruce was also most negatively impacted on western facing slopes. Live stem density was significantly higher (P 0.001) than eastern plots, but live basal area was similar between the two aspects. Atmospheric deposition concentrations of the four main acidic molecules at Mt. Mitchell all peaked in 1998, but decreased by 2012. These reductions, occurring shortly after tightened regulations in the 1990 amendments to the Clean Air Act may have potential implications for increased forest resilience.展开更多
文摘Balsam fir is an important Christmas tree species, especially in eastern Canada. The natural Christmas tree industry faces a challenge in postharvest needle abscission. Though there have been many studies describing the physiological triggers and consequences in postharvest balsam fir, there have been no studies describing morphological or ultrastructural changes. Therefore, the objective of this study was to examine changes in stomata and chloroplast of postharvest needles. Branches were collected from low and high needle abscission resistance balsam fir genotypes, placed in water, and displayed in typical household conditions for 11 weeks. Needle abscission, chlorophyll fluorescence, and water uptake were monitored throughout. Needles stomata and chloroplasts were examined under a scanning and transmission electron microscope, respectively, each week. All branches had increased abscission, decreased chlorophyll fluorescence, and decreased water uptake over time. Needle surfaces accumulated fungal hyphae, especially in stomata. Chloroplasts demonstrated some dysfunction within two weeks, with notable decreases in chloroplast starch and increases in plastoglobulins. Within several weeks thylakoid membranes had been dismantled as chloroplasts transformed into gerontoplasts. All biophysical and structural changes were more pronounced in low needle abscission resistant genotypes. This research identifies a potential role for needle fungi in postharvest needle abscission and confirms the postharvest senescence of chloroplasts. Though it was previously speculated that chloroplasts must senesce postharvest, this study identifies how quickly this process occurs and that it occurs at different rates in contrasting genotypes.
文摘The history of the Black Mountains in North Carolina and the southern Spruce-Fir ecosystem has been fraught with widespread forest decline since the mid 1960’s. Balsam Woolly Adelgid attacks and acidic deposition were two of the most recognized causes of decline. Uncertainty arose about the future of these forests, and projections were made regarding the endangerment or extinction of the endemic Fraser fir ([Pursh] Poiret). This study analyzed data sets from a permanent plot network in the Black Mountains dating 1985, 2002, and 2012. Indications that the Fraser fir population is stabilizing from a “boom-bust” cycle of population growth and has entered the stem exclusion stage of forest stand development are evident. Fir live stem density increased more than 250% from 1985 to 2002, and then declined 40% by 2012 at the highest elevations in the forest. Overall, fir appeared to be more impacted on western facing slopes than eastern ones. The population of red spruce experienced a steady decrease in live stem counts, but an increase in live basal area through all years, and at all elevation classes (1675 m, 1830 m, and 1980 m), indicating a normal progression through stand development. Red spruce was also most negatively impacted on western facing slopes. Live stem density was significantly higher (P 0.001) than eastern plots, but live basal area was similar between the two aspects. Atmospheric deposition concentrations of the four main acidic molecules at Mt. Mitchell all peaked in 1998, but decreased by 2012. These reductions, occurring shortly after tightened regulations in the 1990 amendments to the Clean Air Act may have potential implications for increased forest resilience.
