微小RNA（miRNAs）是一类具有调控功能的内源性非编码单链小分子RNA，长约18-24个核苷酸，与鼻咽癌（nasopharyngeal carcinoma，NPC）的发生发展、诊断以及预后、转归有关。BamHlA右向转录（BamHl A rightward transcripts，BARTs）是E...微小RNA（miRNAs）是一类具有调控功能的内源性非编码单链小分子RNA，长约18-24个核苷酸，与鼻咽癌（nasopharyngeal carcinoma，NPC）的发生发展、诊断以及预后、转归有关。BamHlA右向转录（BamHl A rightward transcripts，BARTs）是EB病毒（EBV）中一组经转录后选择性多剪接的基因产物，存在于EBV所有潜伏状态的miRNA，共22种（miR-BARTl-22），并表达于NPC等EBV感染相关的恶性肿瘤。阐明miR-BARTs在NPC的作用机制对指导临床治疗有重要意义。展开更多
A pair of oligonucleotide primers were designed to amplify the endoflagella gene of L. Interrogans serovar lai. An approximately 840 bp fragment was generated with PCR and inserted into plasmid pUC8,after the fragment...A pair of oligonucleotide primers were designed to amplify the endoflagella gene of L. Interrogans serovar lai. An approximately 840 bp fragment was generated with PCR and inserted into plasmid pUC8,after the fragment and pUC8 were digested respectively with BamHI and PstI. A recombinant plasmid (designated as pLF1 ) was obtained. SDS-PAGE analysis indicated that a 33 kDa was expressed in E. Coli JM103 harboring pLF1 and the expression level of the protein was 11 % of the total bacterial soluble proteins. Western blot analysis showed that the protein band could be recognized by the antiserum against the endoflegella (Axial filament ) of Leptospira interrt,gans serover lai. Nucleotide sequence data showed an open reading frame encoding 282 aminoacids residues,corresponding to a protein of molecular weight 33. 6kDa. Comparison of the deduced endoflagellar subunit protein (flaB) amino acid sequence with flagellins from other bacteria revealed a high level of identity with the Trehoema pallid um flaB proteins. Immunization/protection experiment was performed on the model of BALB/C mice and showed that there was higher survival rate in the group JM103-pLE1 than in the group JM103-pUC8.展开更多
文摘微小RNA（miRNAs）是一类具有调控功能的内源性非编码单链小分子RNA，长约18-24个核苷酸，与鼻咽癌（nasopharyngeal carcinoma，NPC）的发生发展、诊断以及预后、转归有关。BamHlA右向转录（BamHl A rightward transcripts，BARTs）是EB病毒（EBV）中一组经转录后选择性多剪接的基因产物，存在于EBV所有潜伏状态的miRNA，共22种（miR-BARTl-22），并表达于NPC等EBV感染相关的恶性肿瘤。阐明miR-BARTs在NPC的作用机制对指导临床治疗有重要意义。
文摘A pair of oligonucleotide primers were designed to amplify the endoflagella gene of L. Interrogans serovar lai. An approximately 840 bp fragment was generated with PCR and inserted into plasmid pUC8,after the fragment and pUC8 were digested respectively with BamHI and PstI. A recombinant plasmid (designated as pLF1 ) was obtained. SDS-PAGE analysis indicated that a 33 kDa was expressed in E. Coli JM103 harboring pLF1 and the expression level of the protein was 11 % of the total bacterial soluble proteins. Western blot analysis showed that the protein band could be recognized by the antiserum against the endoflegella (Axial filament ) of Leptospira interrt,gans serover lai. Nucleotide sequence data showed an open reading frame encoding 282 aminoacids residues,corresponding to a protein of molecular weight 33. 6kDa. Comparison of the deduced endoflagellar subunit protein (flaB) amino acid sequence with flagellins from other bacteria revealed a high level of identity with the Trehoema pallid um flaB proteins. Immunization/protection experiment was performed on the model of BALB/C mice and showed that there was higher survival rate in the group JM103-pLE1 than in the group JM103-pUC8.
