Objective:To investigate the prevalence of trypanosome infection and their phylogeny in Bandicota indica rats from the cadmium-contaminated area of Mae Sot and the Myanmar border.Methods:Blood samples were taken from ...Objective:To investigate the prevalence of trypanosome infection and their phylogeny in Bandicota indica rats from the cadmium-contaminated area of Mae Sot and the Myanmar border.Methods:Blood samples were taken from 100 animals,and parasite infection was examined by light microscopy observation and polymerase chain reaction(PCR)studies.Results:Trypanosoma spp.infection was found in 20%of the thin blood smear samples.PCR showed positive 623 bp DNA bands in 21 samples(21%).The sequencing analysis showed that all of the samples(100%)had the Trypanasoma lewisi 18 S ribosomal RNA gene.Phylogenetic analysis confirmed that these 16 isolates of Trypanosoma spp.were closely related to Trypanasoma lewisi.Conclusions:Molecular detection using PCR is as effective as conventional light microscopy analysis.This study confirms that trypanosomal infection in rodents is still high;therefore,fleas as their vectors need to be controlled in order to prevent transmission to humans.展开更多
This study investigated some aspects of the reproductive biology of male and female greater bandicoot rats,Bandicota indica,in southern Thailand from September 2004 to September 2006. In females,body,uterine and prepu...This study investigated some aspects of the reproductive biology of male and female greater bandicoot rats,Bandicota indica,in southern Thailand from September 2004 to September 2006. In females,body,uterine and preputial gland weights,occurrences of pregnancies and placental scars,and in males,testicular weights and histology,and sizes of accessory sex glands,were recorded. Pregnancies occurred predominantly,but not exclusively,in the wet season,with a higher incidence pregnancies in the second,than in the first,dry season. Uterine and preputial gland weights tended to be lower in the first,but not the second dry season,with placental scars occurring at all times of year. Males tended to have heavier testes in the wet season but some seminiferous tubules contained sperm even in the dry season. Seminal vesicles,but not prostates and preputial glands,tended to be heavier in animals in the wet season. We conclude that the greater bandicoot rat in southern Thailand shows maximal reproductive activity in the wet season with some reproductive activity,albeit variable from year to year,occurring in the dry season depending upon environmental conditions. This study has also shown that females,as well as males,have large preputial glands,and that males invariably have small testes regardless of the time of year. These observations suggest a similar timing of reproduction,but a different breeding biology and perhaps social organisation,from that of the sympatric ricefield rat,Rattus展开更多
基金granted by Naresuan University,Phitsanulok,Thailand(Grant No.R2559C136).
文摘Objective:To investigate the prevalence of trypanosome infection and their phylogeny in Bandicota indica rats from the cadmium-contaminated area of Mae Sot and the Myanmar border.Methods:Blood samples were taken from 100 animals,and parasite infection was examined by light microscopy observation and polymerase chain reaction(PCR)studies.Results:Trypanosoma spp.infection was found in 20%of the thin blood smear samples.PCR showed positive 623 bp DNA bands in 21 samples(21%).The sequencing analysis showed that all of the samples(100%)had the Trypanasoma lewisi 18 S ribosomal RNA gene.Phylogenetic analysis confirmed that these 16 isolates of Trypanosoma spp.were closely related to Trypanasoma lewisi.Conclusions:Molecular detection using PCR is as effective as conventional light microscopy analysis.This study confirms that trypanosomal infection in rodents is still high;therefore,fleas as their vectors need to be controlled in order to prevent transmission to humans.
文摘This study investigated some aspects of the reproductive biology of male and female greater bandicoot rats,Bandicota indica,in southern Thailand from September 2004 to September 2006. In females,body,uterine and preputial gland weights,occurrences of pregnancies and placental scars,and in males,testicular weights and histology,and sizes of accessory sex glands,were recorded. Pregnancies occurred predominantly,but not exclusively,in the wet season,with a higher incidence pregnancies in the second,than in the first,dry season. Uterine and preputial gland weights tended to be lower in the first,but not the second dry season,with placental scars occurring at all times of year. Males tended to have heavier testes in the wet season but some seminiferous tubules contained sperm even in the dry season. Seminal vesicles,but not prostates and preputial glands,tended to be heavier in animals in the wet season. We conclude that the greater bandicoot rat in southern Thailand shows maximal reproductive activity in the wet season with some reproductive activity,albeit variable from year to year,occurring in the dry season depending upon environmental conditions. This study has also shown that females,as well as males,have large preputial glands,and that males invariably have small testes regardless of the time of year. These observations suggest a similar timing of reproduction,but a different breeding biology and perhaps social organisation,from that of the sympatric ricefield rat,Rattus
