BACKGROUND Helicobacter pylori(H.pylori)is the main pathogen that causes a variety of upper digestive diseases.The drug resistance rate of H.pylori is increasingly higher,and the eradication rate is increasingly lower...BACKGROUND Helicobacter pylori(H.pylori)is the main pathogen that causes a variety of upper digestive diseases.The drug resistance rate of H.pylori is increasingly higher,and the eradication rate is increasingly lower.The antimicrobial resistance of H.pylori is an urgent global problem.It has been confirmed that Banxia Xiexin decoction(BXXXT)demonstrates the effects of treating gastrointestinal diseases,inhibiting H.pylori and protecting gastric mucosa.The purpose of the present study is to further explore the therapeutic effects of BXXXT on drug-resistant H.pylori.AIM To confirm that BXXXT demonstrates therapeutical effects in vivo and in vitro on gastritis mice with drug-resistant H.pylori and explain its mechanism to provide an experimental basis for promoting the application of BXXXT.METHODS The aqueous extract of BXXXT was gained by water decocting method.The inhibitory effect of the aqueous extract on H.pylori was detected by dilution in vitro;drug-resistant H.pylori cells were used to build an acute gastritis model in vivo.Thereafter,the model mice were treated with the aqueous extract of BXXXT.The amount of H.pylori colonization,the repair of gastric mucosal damage,changes of inflammatory factors,apoptosis,etc.,were assessed.In terms of mechanism exploration,the main medicinal compositions of BXXXT aqueous extract and the synergistic bacteriostatic effects they had demonstrated were analyzed using mass spectrometry;the immune function of peripheral blood cells such as CD3+T and CD4+T of mice with gastritis before and after treatment with BXXXT aqueous extract was detected using a flow cytometry;the H.pylori transcriptome and proteome after treatment with BXXXT aqueous extract were detected.Differently expressed genes were screened and verification was performed thereon with knockout expression.RESULTS The minimum inhibitory concentration of BXXXT aqueous extract against H.pylori was 256-512μg/mL.A dose of 28 mg/kg BXXXT aqueous extract treatment produced better therapeutical effects than the standard triple therapy did;the BXXXT aqueous extract have at least 11 ingredients inhibiting H.pylori,including berberine,quercetin,baicalin,luteolin,gallic acid,rosmarinic acid,aloe emodin,etc.,of which berberine,aloe emodin,luteolin and gallic acid have a synergistic effect;BXXXT aqueous extract was found to stimulate the expressions of CD3+T and CD4+T and increase the number of CD4+T/CD8+T in gastritis mice;the detection of transcriptome and proteome,quantitative polymerase chain reaction,Western blotting and knockout verification revealed that the main targets of BXXXT aqueous extract are CFAs related to urea enzymes,and CagA,VacA,etc.CONCLUSION BXXXT aqueous extract could demonstrate good therapeutic effects on drug-resistance H.pylori in vitro and in vivo and its mechanism comes down to the synergistic or additional antibacterial effects of berberine,emodin and luteolin,the main components of the extract;the extract could activate the immune function and enhance bactericidal effects;BXXXT aqueous extract,with main targets of BXXXT aqueous extract related to urease,virulence factors,etc.,could reduce the urease and virulence of H.pylori,weaken its colonization,and reduce its inflammatory damage to the gastric mucosa.展开更多
Objective:The objective of this study is to explore the treatment and compatibility principles of Traditional Chinese Medicine.Materials and Methods:Syndrome differentiation is used to identify the etiology,nature,and...Objective:The objective of this study is to explore the treatment and compatibility principles of Traditional Chinese Medicine.Materials and Methods:Syndrome differentiation is used to identify the etiology,nature,and location of a disease and the relationship between the healthy Qi and pathogenic factors by analyzing and synthesizing the four diagnostics data.The theory of treatment is to determine the principle and methods of treatment according to the results of syndrome differentiation and implement them as a specific prescription.This paper bears witness to the principle universality by analysis of the articles of the Banxia Xiexin Decoction,and it equally has analogical prescriptions in Treatise on Febrile Diseases.Results:The treatment and compatibility principles of Traditional Chinese Medicine are the same,that is,rectifying the deviation on three dimensions,strengthening the healthy Qi,and eliminating pathogenic factors in the light of its general trend.Conclusion:The treatment and compatibility principles of Traditional Chinese Medicine are the same,The treatment and compatibility principles provide a new thinking for clinical treatment.展开更多
目的:观察半夏厚朴汤加味治疗海洛因依赖脱毒后稽延性戒断症状的疗效。方法:198例海洛因依赖者随机分成3组(对照组63例,治疗 A 组65例,治疗 B 组70例),以盐酸洛啡西定脱毒治疗12天后,对照组服模拟制剂,治疗 A 组服用半夏厚朴汤加味60天...目的:观察半夏厚朴汤加味治疗海洛因依赖脱毒后稽延性戒断症状的疗效。方法:198例海洛因依赖者随机分成3组(对照组63例,治疗 A 组65例,治疗 B 组70例),以盐酸洛啡西定脱毒治疗12天后,对照组服模拟制剂,治疗 A 组服用半夏厚朴汤加味60天,治疗 B 组自眼用盐酸洛啡西定脱毒开始时即服用半夏厚朴汤加味,治疗 A、B 组停用中药10天后,分别观察1周内各组稽延性戒断症状并评分,1年后尿检调查复吸情况。结果:稽延性戒断症状评分治疗 B 组低于治疗 A 组(P<0.01),而治疗 A 组低于对照组(P<0.01);1年后复吸率治疗 B 组明显低于对照组和治疗 A 组(P<0.05)。结论:半夏厚朴汤加味可改善海洛因依赖脱毒后稽延性戒断症状,并且早期用药似有降低复吸率的趋势。展开更多
基金the National Natural Science Foundation of China,No.81760739Special Fund Projects for Guide Local Science and Technology Development by the China government,No.GUIKEZY201980042022 Guangxi Graduate Education Innovation Plan Project,No.YCBZ2022071.
文摘BACKGROUND Helicobacter pylori(H.pylori)is the main pathogen that causes a variety of upper digestive diseases.The drug resistance rate of H.pylori is increasingly higher,and the eradication rate is increasingly lower.The antimicrobial resistance of H.pylori is an urgent global problem.It has been confirmed that Banxia Xiexin decoction(BXXXT)demonstrates the effects of treating gastrointestinal diseases,inhibiting H.pylori and protecting gastric mucosa.The purpose of the present study is to further explore the therapeutic effects of BXXXT on drug-resistant H.pylori.AIM To confirm that BXXXT demonstrates therapeutical effects in vivo and in vitro on gastritis mice with drug-resistant H.pylori and explain its mechanism to provide an experimental basis for promoting the application of BXXXT.METHODS The aqueous extract of BXXXT was gained by water decocting method.The inhibitory effect of the aqueous extract on H.pylori was detected by dilution in vitro;drug-resistant H.pylori cells were used to build an acute gastritis model in vivo.Thereafter,the model mice were treated with the aqueous extract of BXXXT.The amount of H.pylori colonization,the repair of gastric mucosal damage,changes of inflammatory factors,apoptosis,etc.,were assessed.In terms of mechanism exploration,the main medicinal compositions of BXXXT aqueous extract and the synergistic bacteriostatic effects they had demonstrated were analyzed using mass spectrometry;the immune function of peripheral blood cells such as CD3+T and CD4+T of mice with gastritis before and after treatment with BXXXT aqueous extract was detected using a flow cytometry;the H.pylori transcriptome and proteome after treatment with BXXXT aqueous extract were detected.Differently expressed genes were screened and verification was performed thereon with knockout expression.RESULTS The minimum inhibitory concentration of BXXXT aqueous extract against H.pylori was 256-512μg/mL.A dose of 28 mg/kg BXXXT aqueous extract treatment produced better therapeutical effects than the standard triple therapy did;the BXXXT aqueous extract have at least 11 ingredients inhibiting H.pylori,including berberine,quercetin,baicalin,luteolin,gallic acid,rosmarinic acid,aloe emodin,etc.,of which berberine,aloe emodin,luteolin and gallic acid have a synergistic effect;BXXXT aqueous extract was found to stimulate the expressions of CD3+T and CD4+T and increase the number of CD4+T/CD8+T in gastritis mice;the detection of transcriptome and proteome,quantitative polymerase chain reaction,Western blotting and knockout verification revealed that the main targets of BXXXT aqueous extract are CFAs related to urea enzymes,and CagA,VacA,etc.CONCLUSION BXXXT aqueous extract could demonstrate good therapeutic effects on drug-resistance H.pylori in vitro and in vivo and its mechanism comes down to the synergistic or additional antibacterial effects of berberine,emodin and luteolin,the main components of the extract;the extract could activate the immune function and enhance bactericidal effects;BXXXT aqueous extract,with main targets of BXXXT aqueous extract related to urease,virulence factors,etc.,could reduce the urease and virulence of H.pylori,weaken its colonization,and reduce its inflammatory damage to the gastric mucosa.
文摘Objective:The objective of this study is to explore the treatment and compatibility principles of Traditional Chinese Medicine.Materials and Methods:Syndrome differentiation is used to identify the etiology,nature,and location of a disease and the relationship between the healthy Qi and pathogenic factors by analyzing and synthesizing the four diagnostics data.The theory of treatment is to determine the principle and methods of treatment according to the results of syndrome differentiation and implement them as a specific prescription.This paper bears witness to the principle universality by analysis of the articles of the Banxia Xiexin Decoction,and it equally has analogical prescriptions in Treatise on Febrile Diseases.Results:The treatment and compatibility principles of Traditional Chinese Medicine are the same,that is,rectifying the deviation on three dimensions,strengthening the healthy Qi,and eliminating pathogenic factors in the light of its general trend.Conclusion:The treatment and compatibility principles of Traditional Chinese Medicine are the same,The treatment and compatibility principles provide a new thinking for clinical treatment.
基金湖北省教育厅重大课题(2000 Z 09003)国家药品监督管理局特殊药品研制项目(TYL20020104)
文摘目的:观察半夏厚朴汤加味治疗海洛因依赖脱毒后稽延性戒断症状的疗效。方法:198例海洛因依赖者随机分成3组(对照组63例,治疗 A 组65例,治疗 B 组70例),以盐酸洛啡西定脱毒治疗12天后,对照组服模拟制剂,治疗 A 组服用半夏厚朴汤加味60天,治疗 B 组自眼用盐酸洛啡西定脱毒开始时即服用半夏厚朴汤加味,治疗 A、B 组停用中药10天后,分别观察1周内各组稽延性戒断症状并评分,1年后尿检调查复吸情况。结果:稽延性戒断症状评分治疗 B 组低于治疗 A 组(P<0.01),而治疗 A 组低于对照组(P<0.01);1年后复吸率治疗 B 组明显低于对照组和治疗 A 组(P<0.05)。结论:半夏厚朴汤加味可改善海洛因依赖脱毒后稽延性戒断症状,并且早期用药似有降低复吸率的趋势。
