[Objectives]To investigate the preventive effects of Wumen Gumi Bao Decoction(WMGBD)on estrogen deficiency-induced bone loss.[Methods]Three-month-old Sprague-Dawley rats were ovariectomized(OVX)and then treated with W...[Objectives]To investigate the preventive effects of Wumen Gumi Bao Decoction(WMGBD)on estrogen deficiency-induced bone loss.[Methods]Three-month-old Sprague-Dawley rats were ovariectomized(OVX)and then treated with WMGBD,and their admixtures for six weeks.The bone trabecular microstructure,bone histopathological examination were determined in the rat femur tissue,and serum biomarkers of bone formation and resorption were analyzed by ELISA,and the protein expressions of Wnt3a,β-catenin,and phosphorylatedβ-catenin(p-β-catenin)were analyzed by Western blot.Statistical analysis was conducted by using one-way analysis of variance(ANOVA)followed by LSD post hoc analysis or independent samples t test using the scientific statistic software SPSS version 20.0.[Results]WMGBD could promote osteosis and ameliorate bone loss to improve the repair of cracked bone trabeculae of OVX rats.Furthermore,WMGBD also could prevent OVX-induced decrease in collagen fibers in the femoral tissue of ovariectomized rats and promote the regeneration of new bone or cartilage tissue,while WMGBD could activate the Wnt3a/β-catenin pathway.[Conclusions]WMGBD could ameliorate estrogen deficiency-induced bone loss via the regulation of Wnt3a/β-catenin pathway.展开更多
基金Supported by Senile Health Research Project of Jiangsu Provincial Health Commission of China(LKZ2023217)Natural Science Foundation of Nanjing University of Traditional Chinese Medicine(XAR2021041)+1 种基金Suzhou Science and Technology Development Plan Project(SYSD2020215,SKY2022202)The Ninth Batch of Suzhou Gusu Health Key Talents Project(GSWS2022107)。
文摘[Objectives]To investigate the preventive effects of Wumen Gumi Bao Decoction(WMGBD)on estrogen deficiency-induced bone loss.[Methods]Three-month-old Sprague-Dawley rats were ovariectomized(OVX)and then treated with WMGBD,and their admixtures for six weeks.The bone trabecular microstructure,bone histopathological examination were determined in the rat femur tissue,and serum biomarkers of bone formation and resorption were analyzed by ELISA,and the protein expressions of Wnt3a,β-catenin,and phosphorylatedβ-catenin(p-β-catenin)were analyzed by Western blot.Statistical analysis was conducted by using one-way analysis of variance(ANOVA)followed by LSD post hoc analysis or independent samples t test using the scientific statistic software SPSS version 20.0.[Results]WMGBD could promote osteosis and ameliorate bone loss to improve the repair of cracked bone trabeculae of OVX rats.Furthermore,WMGBD also could prevent OVX-induced decrease in collagen fibers in the femoral tissue of ovariectomized rats and promote the regeneration of new bone or cartilage tissue,while WMGBD could activate the Wnt3a/β-catenin pathway.[Conclusions]WMGBD could ameliorate estrogen deficiency-induced bone loss via the regulation of Wnt3a/β-catenin pathway.
