[Objectives]To investigate the preventive effects of Wumen Gumi Bao Decoction(WMGBD)on estrogen deficiency-induced bone loss.[Methods]Three-month-old Sprague-Dawley rats were ovariectomized(OVX)and then treated with W...[Objectives]To investigate the preventive effects of Wumen Gumi Bao Decoction(WMGBD)on estrogen deficiency-induced bone loss.[Methods]Three-month-old Sprague-Dawley rats were ovariectomized(OVX)and then treated with WMGBD,and their admixtures for six weeks.The bone trabecular microstructure,bone histopathological examination were determined in the rat femur tissue,and serum biomarkers of bone formation and resorption were analyzed by ELISA,and the protein expressions of Wnt3a,β-catenin,and phosphorylatedβ-catenin(p-β-catenin)were analyzed by Western blot.Statistical analysis was conducted by using one-way analysis of variance(ANOVA)followed by LSD post hoc analysis or independent samples t test using the scientific statistic software SPSS version 20.0.[Results]WMGBD could promote osteosis and ameliorate bone loss to improve the repair of cracked bone trabeculae of OVX rats.Furthermore,WMGBD also could prevent OVX-induced decrease in collagen fibers in the femoral tissue of ovariectomized rats and promote the regeneration of new bone or cartilage tissue,while WMGBD could activate the Wnt3a/β-catenin pathway.[Conclusions]WMGBD could ameliorate estrogen deficiency-induced bone loss via the regulation of Wnt3a/β-catenin pathway.展开更多
xCu/（10NiO-NiFe2O4） cermet and 1BaO-xCu/（10NiO-NiFe2O4） cermet（x=5,10,17） inert anodes were prepared as potential inert anodes for aluminum electrolysis and their corrosion resistance to traditional electrolyte ...xCu/（10NiO-NiFe2O4） cermet and 1BaO-xCu/（10NiO-NiFe2O4） cermet（x=5,10,17） inert anodes were prepared as potential inert anodes for aluminum electrolysis and their corrosion resistance to traditional electrolyte was studied with anodic current density of 1.0 A/cm2 in laboratory electrolysis.The substantial corrosion of metal Cu was observed,many pores appeared on the surface of anode and electrolytes infiltrated inside anodes during the electrolysis.The wear rates of 5Cu/（10NiO-NiFe2O4）,10Cu/（10NiO-NiFe2O4）,17Cu/（10NiO-NiFe2O4）,1BaO-5Cu/（10NiO-NiFe2O4）,1BaO-10Cu/（10NiO-NiFe2O4） and 1BaO-17Cu/（10NiO-NiFe2O4） are 2.15,6.50,8.30,4.88,4.70 and 4.48 cm/a,respectively.The addition of BaO to 10Cu/（10NiO-NiFe2O4） cermet and 17Cu/（10NiO-NiFe2O4） cermet is advantageous because BaO can effectively promote densification and thus improve corrosion resistance.But the addition of BaO to 5Cu/（10NiO-NiFe2O4） cermet is unfavorable to corrosion resistance because additive BaO at the grain boundary of anode accelerates possibly the corrosion of cermet.展开更多
基金Supported by Senile Health Research Project of Jiangsu Provincial Health Commission of China(LKZ2023217)Natural Science Foundation of Nanjing University of Traditional Chinese Medicine(XAR2021041)+1 种基金Suzhou Science and Technology Development Plan Project(SYSD2020215,SKY2022202)The Ninth Batch of Suzhou Gusu Health Key Talents Project(GSWS2022107)。
文摘[Objectives]To investigate the preventive effects of Wumen Gumi Bao Decoction(WMGBD)on estrogen deficiency-induced bone loss.[Methods]Three-month-old Sprague-Dawley rats were ovariectomized(OVX)and then treated with WMGBD,and their admixtures for six weeks.The bone trabecular microstructure,bone histopathological examination were determined in the rat femur tissue,and serum biomarkers of bone formation and resorption were analyzed by ELISA,and the protein expressions of Wnt3a,β-catenin,and phosphorylatedβ-catenin(p-β-catenin)were analyzed by Western blot.Statistical analysis was conducted by using one-way analysis of variance(ANOVA)followed by LSD post hoc analysis or independent samples t test using the scientific statistic software SPSS version 20.0.[Results]WMGBD could promote osteosis and ameliorate bone loss to improve the repair of cracked bone trabeculae of OVX rats.Furthermore,WMGBD also could prevent OVX-induced decrease in collagen fibers in the femoral tissue of ovariectomized rats and promote the regeneration of new bone or cartilage tissue,while WMGBD could activate the Wnt3a/β-catenin pathway.[Conclusions]WMGBD could ameliorate estrogen deficiency-induced bone loss via the regulation of Wnt3a/β-catenin pathway.
基金Project(2005CB623703)supported by the National Basic Research Program of ChinaProject(50721003)supported by the National Natural Science Foundation for Innovation Group of China+1 种基金Project(2008AA030501)supported by the National High-tech Research and Development Program of ChinaProject(201012200021)supported by the Basic Scientific Research Program of Central South University,China
文摘xCu/（10NiO-NiFe2O4） cermet and 1BaO-xCu/（10NiO-NiFe2O4） cermet（x=5,10,17） inert anodes were prepared as potential inert anodes for aluminum electrolysis and their corrosion resistance to traditional electrolyte was studied with anodic current density of 1.0 A/cm2 in laboratory electrolysis.The substantial corrosion of metal Cu was observed,many pores appeared on the surface of anode and electrolytes infiltrated inside anodes during the electrolysis.The wear rates of 5Cu/（10NiO-NiFe2O4）,10Cu/（10NiO-NiFe2O4）,17Cu/（10NiO-NiFe2O4）,1BaO-5Cu/（10NiO-NiFe2O4）,1BaO-10Cu/（10NiO-NiFe2O4） and 1BaO-17Cu/（10NiO-NiFe2O4） are 2.15,6.50,8.30,4.88,4.70 and 4.48 cm/a,respectively.The addition of BaO to 10Cu/（10NiO-NiFe2O4） cermet and 17Cu/（10NiO-NiFe2O4） cermet is advantageous because BaO can effectively promote densification and thus improve corrosion resistance.But the addition of BaO to 5Cu/（10NiO-NiFe2O4） cermet is unfavorable to corrosion resistance because additive BaO at the grain boundary of anode accelerates possibly the corrosion of cermet.
