Objective: To induce callus from the medicinally valuable species, Barringtonia racemosa L.(B. racemosa) whereby the formation of callus is essential for micropropagation studies and in vitro plant secondary metabolit...Objective: To induce callus from the medicinally valuable species, Barringtonia racemosa L.(B. racemosa) whereby the formation of callus is essential for micropropagation studies and in vitro plant secondary metabolites production.Methods: The callus induction potential in B. racemosa was assessed from endosperm explant cultured on different culture media and plant hormonal treatments. Lloyd and Mc Cown's woody plant medium and Murashige and Skoog's medium were used in the study as culture media. On the other hand, various concentrations and combinations of2,4-dichlorophenoxyacetic acid(1.0–2.0 mg/L) and kinetin(0.5–2.5 mg/L) had been incorporated in the culture media to exert the effects of auxin and cytokinin on callus induction.Results: From the present study, it was found that the profuse [(1.681 ± 0.770) g fresh weight,(0.239 ± 0.239) g dry weight] and friable callus formation was optimally produced with desirable morphology and considerable percentage of callus induction(56.70%) in endosperm explants cultured on 1.0 mg/L 2,4-dichlorophenoxyacetic acid and 1.5 mg/L kinetin in Murashige and Skoog's medium.Conclusions: A reliable protocol for inducing callus formation of profuse and friable morphology in endosperm explant of B. racemosa had therefore been successfully established.展开更多
目的:研究红树植物玉蕊的化学成分。方法:用硅胶柱层析,Sephadex LH-20柱层析和核磁共振波谱方法对红树植物玉蕊茎皮乙酸乙酯萃取部位的化学成分进行了分离和结构鉴定。结果:共分离鉴定了5个单体化合物,分别是3,3′-二甲氧基鞣花酸(1),...目的:研究红树植物玉蕊的化学成分。方法:用硅胶柱层析,Sephadex LH-20柱层析和核磁共振波谱方法对红树植物玉蕊茎皮乙酸乙酯萃取部位的化学成分进行了分离和结构鉴定。结果:共分离鉴定了5个单体化合物,分别是3,3′-二甲氧基鞣花酸(1),双氢杨梅素(2),没食子酸(3),Bartogen ic ac id(4)和豆甾醇(5)。结论:化合物1-3首次从玉蕊中分离得到。展开更多
基金Supported by Ministry of Education under Higher Education Department through Fundamental Research Grant Scheme(FRGS)with the grant number of FRGS/1/2012/STW/N03/UITM/02/01
文摘Objective: To induce callus from the medicinally valuable species, Barringtonia racemosa L.(B. racemosa) whereby the formation of callus is essential for micropropagation studies and in vitro plant secondary metabolites production.Methods: The callus induction potential in B. racemosa was assessed from endosperm explant cultured on different culture media and plant hormonal treatments. Lloyd and Mc Cown's woody plant medium and Murashige and Skoog's medium were used in the study as culture media. On the other hand, various concentrations and combinations of2,4-dichlorophenoxyacetic acid(1.0–2.0 mg/L) and kinetin(0.5–2.5 mg/L) had been incorporated in the culture media to exert the effects of auxin and cytokinin on callus induction.Results: From the present study, it was found that the profuse [(1.681 ± 0.770) g fresh weight,(0.239 ± 0.239) g dry weight] and friable callus formation was optimally produced with desirable morphology and considerable percentage of callus induction(56.70%) in endosperm explants cultured on 1.0 mg/L 2,4-dichlorophenoxyacetic acid and 1.5 mg/L kinetin in Murashige and Skoog's medium.Conclusions: A reliable protocol for inducing callus formation of profuse and friable morphology in endosperm explant of B. racemosa had therefore been successfully established.
文摘目的:研究红树植物玉蕊的化学成分。方法:用硅胶柱层析,Sephadex LH-20柱层析和核磁共振波谱方法对红树植物玉蕊茎皮乙酸乙酯萃取部位的化学成分进行了分离和结构鉴定。结果:共分离鉴定了5个单体化合物,分别是3,3′-二甲氧基鞣花酸(1),双氢杨梅素(2),没食子酸(3),Bartogen ic ac id(4)和豆甾醇(5)。结论:化合物1-3首次从玉蕊中分离得到。
