The stem of Ginkgo biloba were cultured on MS,modified MS and DCR basal medium,supplemented with various concentration of hormones—NAA,ZT (zeatin) and two types of nurture-CH (casein hydrolysate) and Ade (adenine). T...The stem of Ginkgo biloba were cultured on MS,modified MS and DCR basal medium,supplemented with various concentration of hormones—NAA,ZT (zeatin) and two types of nurture-CH (casein hydrolysate) and Ade (adenine). The aim was to study the effects of various basal medium,different hormones and nurtures on the growth and development of Ginkgo biloba stem. The result showed that the modified MS with CH 500 mg·L -1 was the best medium for inducing the development of axillary buds;the medium with NAA,ZT and Ade promoted axillary buds to be callus and inhibited them developing into shoots. The shoot rate was 89.3%. After cultured for 40 days,the clustered buds were emerged from axillary buds. The detached buds from clustered buds were cultured on modified MS basal medium or supplemented with CH 500 mg·L -1 . The shoots grew well,and the reproduction coefficient was 3. The shoots from apical buds were also reproduced,and the reproduction coefficient was 4. The shoots were induced to root on MS+NAA 0.1 mg·L -1 medium,and the rooting rate was 33.3%.展开更多
Different nutrient media can affect in vitro culturing proto- cols, and experimentation under varied growth conditions is valuable in plants where in vitro methods are in preliminary stages. We carried out the first i...Different nutrient media can affect in vitro culturing proto- cols, and experimentation under varied growth conditions is valuable in plants where in vitro methods are in preliminary stages. We carried out the first in vitro propagation studies for the endangered species Caragana fruticosa （Fabaceae）. We evaluated various nulrient media for their im- pact on shoot elongation and axillary bud proliferation using different concentrations of 6-benzylaminopurine （BA） and n-naphthaleneacetic acid （NAA）. Shoot elongation was evaluated based on adventitious shoot primary culture and subculture regeneration from Caragana seedlings. Our goal was to improve both micropropagation and regeneration in C. fruticosa. MS nutrient media was superior to 1/2MS macronutrients, DKW, QL, and WPM for shoot elongation and axillary shoot prolifera- tion. Shoots grown on 1/2MS and WPM exhibited some chlorosis, and shoots on QL produced larger leavers than plants growing on normal medium. The shoot proliferation coefficient on MS media supplemented with 2.22 μM BA and 0.44μM BA ＋ 2.69 μM NAA was significantly higher than that with other treatments in the primary culture. Shoots on 2.22 μM BA showed a higher proliferation coefficient （3.17） than others in the subculture. Shoots were rooted on 1/2MS medium with the addition of different concentrations of NAA. The optimal concenWation for rooting was 0.27 μM NAA （74%）. Roots exhibited many stout and long root hairs. Survivl of established plentlets was 82% at 30 days after transfer to soil. Plants established in the green house showed normal growth and displayed no apparent morphological differences compared to stock plants.展开更多
文摘The stem of Ginkgo biloba were cultured on MS,modified MS and DCR basal medium,supplemented with various concentration of hormones—NAA,ZT (zeatin) and two types of nurture-CH (casein hydrolysate) and Ade (adenine). The aim was to study the effects of various basal medium,different hormones and nurtures on the growth and development of Ginkgo biloba stem. The result showed that the modified MS with CH 500 mg·L -1 was the best medium for inducing the development of axillary buds;the medium with NAA,ZT and Ade promoted axillary buds to be callus and inhibited them developing into shoots. The shoot rate was 89.3%. After cultured for 40 days,the clustered buds were emerged from axillary buds. The detached buds from clustered buds were cultured on modified MS basal medium or supplemented with CH 500 mg·L -1 . The shoots grew well,and the reproduction coefficient was 3. The shoots from apical buds were also reproduced,and the reproduction coefficient was 4. The shoots were induced to root on MS+NAA 0.1 mg·L -1 medium,and the rooting rate was 33.3%.
基金supported by the Key Technologies R&D Program of China during 2006-2010 (2006BAD03A04)the Fundamental Research Funds for the Central Universities(DL10BA04)
文摘Different nutrient media can affect in vitro culturing proto- cols, and experimentation under varied growth conditions is valuable in plants where in vitro methods are in preliminary stages. We carried out the first in vitro propagation studies for the endangered species Caragana fruticosa （Fabaceae）. We evaluated various nulrient media for their im- pact on shoot elongation and axillary bud proliferation using different concentrations of 6-benzylaminopurine （BA） and n-naphthaleneacetic acid （NAA）. Shoot elongation was evaluated based on adventitious shoot primary culture and subculture regeneration from Caragana seedlings. Our goal was to improve both micropropagation and regeneration in C. fruticosa. MS nutrient media was superior to 1/2MS macronutrients, DKW, QL, and WPM for shoot elongation and axillary shoot prolifera- tion. Shoots grown on 1/2MS and WPM exhibited some chlorosis, and shoots on QL produced larger leavers than plants growing on normal medium. The shoot proliferation coefficient on MS media supplemented with 2.22 μM BA and 0.44μM BA ＋ 2.69 μM NAA was significantly higher than that with other treatments in the primary culture. Shoots on 2.22 μM BA showed a higher proliferation coefficient （3.17） than others in the subculture. Shoots were rooted on 1/2MS medium with the addition of different concentrations of NAA. The optimal concenWation for rooting was 0.27 μM NAA （74%）. Roots exhibited many stout and long root hairs. Survivl of established plentlets was 82% at 30 days after transfer to soil. Plants established in the green house showed normal growth and displayed no apparent morphological differences compared to stock plants.
