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神经前体细胞巢蛋白在大鼠脊髓损伤后的表达 被引量:6
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作者 李俊岑 丁培培 +3 位作者 刘姿辰 张剑 梁楠 张晓 《中国康复医学杂志》 CAS CSCD 北大核心 2010年第9期833-837,共5页
目的:探讨成年大鼠脊髓损伤后损伤(SCI)区局部巢蛋白(nestin)的表达及意义。方法:应用Allen法建立大鼠SCI模型,行为学评分采用BBB评分,用病理学和免疫组织化学方法检测脊髓在不同时段的病理改变和nestin的表达变化。结果:伤后第1天,脊... 目的:探讨成年大鼠脊髓损伤后损伤(SCI)区局部巢蛋白(nestin)的表达及意义。方法:应用Allen法建立大鼠SCI模型,行为学评分采用BBB评分,用病理学和免疫组织化学方法检测脊髓在不同时段的病理改变和nestin的表达变化。结果:伤后第1天,脊髓实质灶状出血,小血管栓塞,部分神经细胞细胞核碎裂,见损伤区附近、软脊髓膜下的白质和脊髓中央管区有nestin表达,BBB评分低,随后增加,1—2周恢复幅度加大。第3天后损伤灶出现大量胶质细胞,损伤组织液化。第5天后液化灶逐渐扩大,出血减少,阳性神经元和阳性反应的平均积分光密度值达到高峰(P<0.05)。第7天后神经细胞退行性变更为严重,部分神经细胞崩解仅留其轮廓,胶质细胞增生明显。2周后出血已基本吸收,以损伤处为中心,囊腔开始形成,nestin表达明显下调(P<0.05)。结论:脊髓损伤可诱导损伤区周围短暂的nestin阳性表达,nestin可能在脊髓损伤后的再生与修复中起重要作用。 展开更多
关键词 脊髓损伤 巢蛋白 Allen撞击器 basso beattie bresnahan运动功能评分
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神经行为学检测和改良BBB运动功能评定在脑瘫大鼠运动功能评定中的应用 被引量:14
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作者 李晓捷 郭岚敏 《中国康复医学杂志》 CAS CSCD 北大核心 2008年第12期1067-1070,共4页
目的:探讨脑瘫大鼠运动功能评价的最佳方法。方法:采用孕鼠腹腔注射LPS制备脑瘫动物模型,随机选取对照组(A组)足月仔鼠60只,LPS组足月仔鼠120只分为早期干预组(B1组)和非干预组(B2组)各60只。各组于生后25d进行神经行为学检测,B1组中检... 目的:探讨脑瘫大鼠运动功能评价的最佳方法。方法:采用孕鼠腹腔注射LPS制备脑瘫动物模型,随机选取对照组(A组)足月仔鼠60只,LPS组足月仔鼠120只分为早期干预组(B1组)和非干预组(B2组)各60只。各组于生后25d进行神经行为学检测,B1组中检测出的CP鼠(B1CP组)继续早期干预,B2组中检测出的CP鼠(B2CP组)常规饲养,A组中随机选取10只鼠作为对照组(A′组);A′组、B1CP组和B2CP组仔鼠分别于25d及42d进行神经行为学检测和运动功能评定。结果:①25dB1组鉴定出7只CP鼠,B2组13只CP鼠,A组中无CP鼠。②B1CP组第25天与第42天各项检测结果比较:悬吊试验、斜坡试验、旷场实验、拒俘反应及改良的BBB运动功能评分均有显著性差异(P﹤0.01);B2CP组和A′组大鼠25d与42d各项检测结果比较无显著性差异(P﹥0.05)。结论:①神经行为学检测可有效鉴定CP大鼠的运动功能。②改良BBB运动功能评定适用于CP大鼠的运动功能评定。③联合应用神经行为学检测与改良BBB运动功能评定可全面反映CP大鼠运动功能状况。 展开更多
关键词 早期干预 宫内感染 脑性瘫痪 运动功能评定 bassobeattiebresnahan运动功能评定
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Hydrogen-rich saline injection into the subarachnoid cavity within 2 weeks promotes recovery after acute spinal cord injury 被引量:13
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作者 Jian-long Wang Qing-shan Zhang +4 位作者 Kai-di Zhu Jian-feng Sun Ze-peng Zhang Jian-wen Sun Ke-xiang Zhang 《Neural Regeneration Research》 SCIE CAS CSCD 2015年第6期958-964,共7页
Hydrogen can relieve tissue-damaging oxidative stress, inflammation and apoptosis. Injection of hydrogen-rich saline is an effective method for transporting molecular hydrogen. We hypothesized that hydrogen-rich salin... Hydrogen can relieve tissue-damaging oxidative stress, inflammation and apoptosis. Injection of hydrogen-rich saline is an effective method for transporting molecular hydrogen. We hypothesized that hydrogen-rich saline would promote the repair of spinal cord injury induced by Allen's method in rats. At 0.5, 1, 2, 4, 8, 12 and 24 hours after injury, then once daily for 2 weeks, 0.25 mL/kg hydrogen-rich saline was infused into the subarachnoid space through a catheter. Results at 24 hours, 48 hours, 1 week and 2 weeks after injury showed that hydrogen-rich saline markedly reduced cell death, inflammatory cell infiltration, serum malondialdehyde content, and caspa se-3 immunoreactivity, elevated serum superoxide dismutase activity and calcitonin gene-related peptide immunoreactivity, and improved motor function in the hindlimb. The present study confirms that hydrogen-rich saline injected within 2 weeks of injury effectively contributes to the repair of spinal cord injury in the acute stage. 展开更多
关键词 nerve regeneration spinal cord injury hydrogen-rich saline reactive oxygen species physiological saline oxidative stress basso beattie and bresnahan score MALONDIALDEHYDE SUPEROXIDEDISMUTASE calcitonin gene-related peptide caspase-3 neural regeneration
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Electroacupuncture promotes the recovery of motor neuron function in the anterior horn of the injured spinal cord 被引量:15
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作者 Jian-hui Yang Jian-guo Lv +1 位作者 Hui Wang Hui-yong Nie 《Neural Regeneration Research》 SCIE CAS CSCD 2015年第12期2033-2039,共7页
Acupuncture has been shown to lessen the inflammatory reaction after acute spinal cord injury and reduce secondary injury.However,the mechanism of action remains unclear.In this study,a rat model of spinal cord injury... Acupuncture has been shown to lessen the inflammatory reaction after acute spinal cord injury and reduce secondary injury.However,the mechanism of action remains unclear.In this study,a rat model of spinal cord injury was established by compressing the T8-9 segments using a modified Nystrom method.Twenty-four hours after injury,Zusanli(ST36),Xuanzhong(GB39),Futu(ST32)and Sanyinjiao(SP6)were stimulated with electroacupuncture.Rats with spinal cord injury alone were used as controls.At 2,4 and 6 weeks after injury,acetylcholinesterase(ACh E)activity at the site of injury,the number of medium and large neurons in the spinal cord anterior horn,glial cell line-derived neurotrophic factor(GDNF)m RNA expression,and Basso,Beattie and Bresnahan locomotor rating scale scores were greater in the electroacupuncture group compared with the control group.These results demonstrate that electroacupuncture increases ACh E activity,up-regulates GDNF m RNA expression,and promotes the recovery of motor neuron function in the anterior horn after spinal cord injury. 展开更多
关键词 nerve regeneration spinal cord injury ELECTROACUPUNCTURE spine injury secondaryinjury ACUPOINT motor neurons ACETYLCHOLINESTERASE glial cell line-derived neurotrophic factor inclined board test basso beattie and bresnahan locomotor rating scale functional recovery neuralregeneration
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Effects of decompression joint Governor Vessel electro-acupuncture on rats with acute upper cervical spinal cord injury 被引量:8
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作者 Yan-Lei Wang Ying-Na Qi +5 位作者 Wei Wang Chun-Ke Dong Ping Yi Feng Yang Xiang-Sheng Tang Ming-Sheng Tan 《Neural Regeneration Research》 SCIE CAS CSCD 2018年第7期1241-1246,共6页
Decompression is the major therapeutic strategy for acute spinal cord injury,but there is some debate about the time window for decompression following spinal cord injury.An important goal and challenge in the treatme... Decompression is the major therapeutic strategy for acute spinal cord injury,but there is some debate about the time window for decompression following spinal cord injury.An important goal and challenge in the treatment of spinal cord injury is inhibiting or reversing secondary injury.Governor Vessel electroacupuncture can improve symptoms of spinal cord injury by inhibiting cell apoptosis and improving the microenvironment of the injured spinal cord.In this study,Governor Vessel electroacupuncture combined with decompression at different time points was used to treat acute spinal cord injury.The rat models were established by inserting a balloon catheter into the atlanto-occipital space.The upper cervical spinal cord was compressed for 12 or 48 hours prior to decompression.Electroacupuncture was conducted at the acupoints Dazhui(GV14) and Baihui(GV 20)(2 Hz,15 minutes) once a day for 14 consecutive days.Compared with decompression alone,hind limb motor function recovery was superior after decompression for 12 and 48 hours combined with electroacupuncture.However,the recovery of motor function was not significantly different at 14 days after treatment in rats receiving decompression for 12 hours.Platelet-activating factor levels and caspase-9 protein expression were significantly reduced in rats receiving electroacupuncture compared with decompression alone.These findings indicate that compared with decompression alone,Governor Vessel electroacupuncture combined with delayed decompression(48 hours) is more effective in the treatment of upper cervical spinal cord injury.Governor Vessel electroacupuncture combined with early decompression(12 hours) can accelerate the recovery of nerve movement in rats with upper cervical spinal cord injury.Nevertheless,further studies are necessary to confirm whether it is possible to obtain additional benefit compared with early decompression alone. 展开更多
关键词 nerve regeneration acute spinal cord injury decompression Governor Vessel electroacupuncture platelet-activating factor apoptosis methylprednisolone caspase family upper cervical spine animal model basso beattie and bresnahan locomotor scale neural regeneration
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Globose basal cells for spinal cord regeneration 被引量:4
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作者 Durai Murugan Muniswami Indirani Kanakasabapathy George Tharion 《Neural Regeneration Research》 SCIE CAS CSCD 2017年第11期1895-1904,共10页
Spinal cord injury (SCI) is a devastating condition with loss of motor and sensory functions below the injury level. Cell based therapies are experimented in pre-clinical studies around the world. Neural stem cells ... Spinal cord injury (SCI) is a devastating condition with loss of motor and sensory functions below the injury level. Cell based therapies are experimented in pre-clinical studies around the world. Neural stem cells are located intra-craniafly in subventricular zone and hippocampus which are highly invasive sourc- es. The olfactory epithelium is a neurogenic tissue where neurogenesis takes place throughout the adult life by a population of stem/progenitor cells. Easily accessible olfactory neuroepithelial stem/progenitor cells are an attractive cell source for transplantation in SCI. Globose basal cells (GBCs) were isolated from rat olfactory epithelium, characterized by flow cytometry and immunohistochemically. These ceils were further studied for neurosphere formation and neuronal induction. T10 laminectomy was done to create drop-weight SCI in rats. On the 9th day following SCI, 5 × 105 cells were transplanted into injured rat spinal cord. The outcome of transplantation was assessed by the Basso, Beattie and Bresnahan (BBB) locomotor rating scale, motor evoked potential and histological observation. GBCs expressed neural stem cell markers nestin, SOX2, NCAM and also mesenchymal stem cell markers (CD29, CD54, CD90, CD73, CD105). These cells formed neurosphere, a culture characteristics of NSCs and on induction, differentiated cells expressed neuronal markers ~III tubulin, microtubule-associated protein 2, neuronal nuclei, and neurofilament. GBCs transplanted rats exhibited hindlimb motor recovery as confirmed by BBB score and gastrocnemius muscle electromyography amplitude was increased compared to controls. Green fluorescent protein labelled GBCs survived around the injury epicenter and differentiated into βⅢ tubulin-immunoreactive neuron-like cells. GBCs could be an alternative to NSCs from an accessible source for autologous neurotransplantation after SCI without ethical issues. 展开更多
关键词 nerve regeneration the basso beattie and bresnahan (BBB) locomotor scale score BBB score EMG globose basal cells spinal cord injury TRANSPLANTATION neural stem cell marker neural regeneration
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Neurofilament 200 expression in a rat model of complete spinal cord injury following growth-associated protein-43 treatment
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作者 Yanping Duan Dongkui Zhang +5 位作者 Yingchun Ba Yun Yuan Jun Sun Dengli Fu Ran Zhang Jinde Wang 《Neural Regeneration Research》 SCIE CAS CSCD 2009年第11期827-831,共5页
BACKGROUND: Growth-associated protein-43 (GAP-43) expression in the nervous system has been demonstrated to promote neural regeneration, neuronal growth and development, as well as synaptic reconstruction. Neurofil... BACKGROUND: Growth-associated protein-43 (GAP-43) expression in the nervous system has been demonstrated to promote neural regeneration, neuronal growth and development, as well as synaptic reconstruction. Neurofilament 200 (NF200) expression could reflect degree of injury and repair in injured spinal axons. OBJECTIVE: To observe NF200 expression changes in a rat model of complete spinal cord injury following GAP-43 treatment and to explore the effects of GAP-43 following spinal cord injury. DESIGN, TIME AND SETTING: A randomized, controlled, animal experiment was performed at the Laboratory of Histology and Embryology of Kunming Medical University between March 2007 and October 2008. MATERIALS: GAP-43 and GAP-43 antibody were provided by Beijing Boao Biology, China; mouse anti-rat NF200 antibody was purchased from Chemicon, USA. METHODS: Female, 8-week-old, Sprague Dawley rats were randomly assigned into three groups following complete spinal cord injury, with 20 animals in each group: GAP-43 antibody, GAP-43, and model groups. In addition, each group was subdivided into four subgroups according to sampling time after modeling, Le., 3-, 5-, 9-, and 15-day groups, with 5 rats in each group. GAP-43 antibody or GAP-43 was injected into injury sites of the spinal cord, 5 μg/0.2 mL, respectively, twice daily for three consecutive days, followed by three additional days of injection, once daily. The model group did not receive any treatment following injury. MAIN OUTCOME MEASURES: NF200 expression in the damaged spinal area at different stages was detected by immunohistochemistry; lower limb motion function following injury was evaluated using the Basso, Beattie and Bresnahan (BBB) locomotor rating scale. RESULTS: NF200 expression was significantly reduced in the GAP-43 antibody group, compared with GAP-43 and model groups, at 3 and 5 days after spinal cord injury (P 〈 0.05). In addition, the model group expressed significantly less NF200 than the GAP-43 group (P 〈 0.05). BBB scores from the GAP-43 antibody and model groups were remarkably less than the GAP-43 group (P 〈 0.05). At 9 and 15 days of injury after drug withdrawal, NF200 expression was increased in the GAP-43 antibody group, and NF200 expression and BBB scores in the GAP-43 antibody and GAP-43 groups were significantly greater than in the model group (P 〈 0.05). In particular, the GAP-43 group exhibited greater BBB scores than the GAP-43 antibody group at day 9 (P 〈 0.05). CONCLUSION: GAP-43 promoted NF200 expression and recovery of lower limb function. Early administration of GAP-43 antibody produced reversible nerve inhibition, which was rapidly restored following withdrawal. 展开更多
关键词 spinal cord injury growth-associated protein-43 neurofilament 200 basso beattie and bresnahan locomotor rating scale
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