[Objective] The aim of the research was to study the effect of the ice bath time after heat shock and the incubation time on the transformation efficacy,and to establish a simple and quick transformation method.[Metho...[Objective] The aim of the research was to study the effect of the ice bath time after heat shock and the incubation time on the transformation efficacy,and to establish a simple and quick transformation method.[Method]Competent cells were prepared with two buffer solutions;with the ice bath time after heat shock and the recovery time as the variables,the relationship between these two factors and transformation efficacy was studied.[Result]The transformation efficacy was the best when the ice bath time was 2 min and the recovery time was 30 or 40 min;when the ice bath time and the recovery time was 0 min,a certain amount of transformants still could be obtained.[Conclusion]The ice bath time after heat shock and the recovery time had certain impact on transformation efficacy,but they were not the decisive factors.Therefore,in the general transformation experiment,these two steps could be omitted.展开更多
<strong>Purpose:</strong> The potential dangers of infant bathing have an effect not only on the infant’s body and life but also on the formation of emotional bonds in the parent-child relationship. This ...<strong>Purpose:</strong> The potential dangers of infant bathing have an effect not only on the infant’s body and life but also on the formation of emotional bonds in the parent-child relationship. This study will explore actual dangers experienced by mothers and families. <strong>Method: </strong>We distributed an anonymous, self-reported questionnaire at infants’ 18-month health checkup, which was collected via postal service. 304 valid responses were received and formed our sample data. Descriptive statistical analysis was performed for each category surveyed. Inferential statistics were used to compare the dangerous incidents experienced with regard to differences between primipara/multipara and the presence/absence of guidance concerning such incidents. This study was approved by the research ethics committees of the affiliated university. <strong>Results:</strong> 70% of mothers and families experienced incidents while bathing their infants, with the most common incidents consisting of, in order of frequency, near immersion of the face, actual immersion of the face, and falling into the bath. The most common situations reported when incidents occurred were bathing the infant by oneself, the infant moving unexpectedly, or taking care of other children, in that order. Approximately 70% of mothers and families had not received instruction on the potential dangers or how to prevent them. <strong>C</strong><strong>onclusion:</strong> This study has elucidated the dangerous situations experienced in the course of bathing infants in the home and the measures taken to prevent such situations. Instructive tools are needed which can be used to easily provide mothers and families with correct information.展开更多
Using the order parameter of seismicity defined in natural time, we suggest a simple model for the expla- nation of Bath law, according to which a mainshock differs in magnitude from its largest aftershock by approxim...Using the order parameter of seismicity defined in natural time, we suggest a simple model for the expla- nation of Bath law, according to which a mainshock differs in magnitude from its largest aftershock by approximately 1.2 regardless of the mainshock magnitude. In addition, the validity of Bath law is studied in the Global Centroid Moment Tensor catalogue by using two different aftershock definitions. It is found that the mean of this difference, when considering all the pairs mainshock-largest aftershock, does not markedly differ from 1.2 and the corresponding distributions do not depend on the mainshock＇s magnitude threshold in a statistically significant manner. Finally, the analysis of the cumulative distribution functions provides evidence in favour of the proposed model.展开更多
目的探讨不同坐浴时间对肛瘘术后创面愈合的影响,寻求适宜的坐浴时间。方法将40例肛瘘术后病人按随机双盲分为两组,每组各20人,分别用0.02%高锰酸钾溶液坐浴5 m in(实验组)和20 m in(对照组)1个疗程(7 d),对比创面疼痛、水肿、创面肉芽...目的探讨不同坐浴时间对肛瘘术后创面愈合的影响,寻求适宜的坐浴时间。方法将40例肛瘘术后病人按随机双盲分为两组,每组各20人,分别用0.02%高锰酸钾溶液坐浴5 m in(实验组)和20 m in(对照组)1个疗程(7 d),对比创面疼痛、水肿、创面肉芽生长情况以及创面愈合时间。结果实验组病人疼痛、水肿程度轻,创面肉芽组织生长良好,创面愈合时间短于对照组。结论适宜缩短坐浴时间可减轻病人创面疼痛、水肿,促进创口早期愈合。展开更多
基金Supported by Foundation for Returned Scholars of Hebei Province(2010)Research Fund for the Doctoral Program of Hebei Normal University(L2009B13)~~
文摘[Objective] The aim of the research was to study the effect of the ice bath time after heat shock and the incubation time on the transformation efficacy,and to establish a simple and quick transformation method.[Method]Competent cells were prepared with two buffer solutions;with the ice bath time after heat shock and the recovery time as the variables,the relationship between these two factors and transformation efficacy was studied.[Result]The transformation efficacy was the best when the ice bath time was 2 min and the recovery time was 30 or 40 min;when the ice bath time and the recovery time was 0 min,a certain amount of transformants still could be obtained.[Conclusion]The ice bath time after heat shock and the recovery time had certain impact on transformation efficacy,but they were not the decisive factors.Therefore,in the general transformation experiment,these two steps could be omitted.
文摘<strong>Purpose:</strong> The potential dangers of infant bathing have an effect not only on the infant’s body and life but also on the formation of emotional bonds in the parent-child relationship. This study will explore actual dangers experienced by mothers and families. <strong>Method: </strong>We distributed an anonymous, self-reported questionnaire at infants’ 18-month health checkup, which was collected via postal service. 304 valid responses were received and formed our sample data. Descriptive statistical analysis was performed for each category surveyed. Inferential statistics were used to compare the dangerous incidents experienced with regard to differences between primipara/multipara and the presence/absence of guidance concerning such incidents. This study was approved by the research ethics committees of the affiliated university. <strong>Results:</strong> 70% of mothers and families experienced incidents while bathing their infants, with the most common incidents consisting of, in order of frequency, near immersion of the face, actual immersion of the face, and falling into the bath. The most common situations reported when incidents occurred were bathing the infant by oneself, the infant moving unexpectedly, or taking care of other children, in that order. Approximately 70% of mothers and families had not received instruction on the potential dangers or how to prevent them. <strong>C</strong><strong>onclusion:</strong> This study has elucidated the dangerous situations experienced in the course of bathing infants in the home and the measures taken to prevent such situations. Instructive tools are needed which can be used to easily provide mothers and families with correct information.
文摘Using the order parameter of seismicity defined in natural time, we suggest a simple model for the expla- nation of Bath law, according to which a mainshock differs in magnitude from its largest aftershock by approximately 1.2 regardless of the mainshock magnitude. In addition, the validity of Bath law is studied in the Global Centroid Moment Tensor catalogue by using two different aftershock definitions. It is found that the mean of this difference, when considering all the pairs mainshock-largest aftershock, does not markedly differ from 1.2 and the corresponding distributions do not depend on the mainshock＇s magnitude threshold in a statistically significant manner. Finally, the analysis of the cumulative distribution functions provides evidence in favour of the proposed model.
文摘目的探讨不同坐浴时间对肛瘘术后创面愈合的影响,寻求适宜的坐浴时间。方法将40例肛瘘术后病人按随机双盲分为两组,每组各20人,分别用0.02%高锰酸钾溶液坐浴5 m in(实验组)和20 m in(对照组)1个疗程(7 d),对比创面疼痛、水肿、创面肉芽生长情况以及创面愈合时间。结果实验组病人疼痛、水肿程度轻,创面肉芽组织生长良好,创面愈合时间短于对照组。结论适宜缩短坐浴时间可减轻病人创面疼痛、水肿,促进创口早期愈合。
