Objective Cerebral ischemia/reperfusion(I/R)is a potential factor for lethal injury,and currently lacks effective remedies.Bauhinia championii extracts(BCEs)have been reported to exhibit anti-oxidative and anti-hypoxi...Objective Cerebral ischemia/reperfusion(I/R)is a potential factor for lethal injury,and currently lacks effective remedies.Bauhinia championii extracts(BCEs)have been reported to exhibit anti-oxidative and anti-hypoxia properties.The current work aimed to study whether BCE could alleviate neuronal injury caused by I/R.Methods To investigate the protective effects of BCE,oxygen-glucose deprivation/reperfusion(OGD/R)was applied to the HT22 cell line in vitro and to a cerebral I/R mouse model in vivo.Results Under OGD/R,the survival of HT22 cells was significantly prolonged after treatment with BCE.In vivo,BCE significantly reduced the infarct area and decreased neuronal apoptosis caused by I/R.It was further found that OGD/R could trigger endoplasmic reticulum(ER)stress and induce ER stress-mediated neuronal apoptosis in vivo and in vitro,while BCE could effectively alleviate ER stress and neuronal apoptosis.Conclusion These results suggested that BCE exhibits neuroprotective effects by reducing ER stress-mediated apoptosis after cerebral I/R injury.BCE may therefore be an effective therapeutic regimen against cerebral I/R damage.展开更多
基金Health Commission of Hubei Province(No.ZY2021M014)the National Natural Science Foundation of China(No.81873725)+2 种基金Basic Scientific Research Funds of Department of Education of Zhejiang Province(No.KYYB202007)Zhejiang Basic Public Welfare Research Program(No.LGF22H280016)the“13th Five-Year”Chinese Medicine Key Discipline in Zhejiang Province-Chinese Medicine Quality and Functional Evaluation(No.2017-XK-A43).
文摘Objective Cerebral ischemia/reperfusion(I/R)is a potential factor for lethal injury,and currently lacks effective remedies.Bauhinia championii extracts(BCEs)have been reported to exhibit anti-oxidative and anti-hypoxia properties.The current work aimed to study whether BCE could alleviate neuronal injury caused by I/R.Methods To investigate the protective effects of BCE,oxygen-glucose deprivation/reperfusion(OGD/R)was applied to the HT22 cell line in vitro and to a cerebral I/R mouse model in vivo.Results Under OGD/R,the survival of HT22 cells was significantly prolonged after treatment with BCE.In vivo,BCE significantly reduced the infarct area and decreased neuronal apoptosis caused by I/R.It was further found that OGD/R could trigger endoplasmic reticulum(ER)stress and induce ER stress-mediated neuronal apoptosis in vivo and in vitro,while BCE could effectively alleviate ER stress and neuronal apoptosis.Conclusion These results suggested that BCE exhibits neuroprotective effects by reducing ER stress-mediated apoptosis after cerebral I/R injury.BCE may therefore be an effective therapeutic regimen against cerebral I/R damage.
