OBJECTIVE: To investigate the effects of Bazhen Pills on quality of life and immune function of patients undergoing chemotherapy after gallbladder cancer surgery. METHODS: A total of 64 patients with gallbladder cance...OBJECTIVE: To investigate the effects of Bazhen Pills on quality of life and immune function of patients undergoing chemotherapy after gallbladder cancer surgery. METHODS: A total of 64 patients with gallbladder cancer treated by surgery and postoperative basic nutritional support and symptomatic treatment in our hospital were selected and randomly divided into the control group and the observation group, with 32 cases in each group.The control group was treated with chemotherapy from 4^(th) to 6^(th) week after surgery. The observation group was treated by Bazhen Pills on the basis of the treatment in the control group. The blood cell count, immune function,TCM symptom scores, KPS scores, quality of life and adverse reactions were compared between the 2 groups.RESULTS: The WBC and PLT in 2 groups were decreased after the treatment, and the data in the observation group were higher than that in the control group(P < 0.05). The levels of CD3^+, CD4^+ and CD8^+ in the 2 groups were increased after the treatment(P < 0.05), and the data in the observation group were higher than that in the control group(P < 0.05). TCM syndrome scores of the 2 groups were decreased after the treatment(P < 0.05),and the KPS scores were increased after the treatment(P < 0.05). The improvement degrees of the 2 scores in the observation group were higher than that of the control group(P < 0.05). The fatigue symptom scores of the 2 groups were decreased while scores of physical function, role function and overall health were increased after the treatment(P < 0.05). The scores of the observation group were better than those of the control group(P < 0.05). The total incidence of grade I-II hematological toxicity and grade I-II gastrointestinal reactions in the observation group was lower than that in the control group(21.88% vs 50.00%)(P < 0.05). CONCLUSION: Bazhen Pills can effectively relieve clinical symptoms of patients undergoing chemotherapy after gallbladder cancer surgery, and improve their immune function and quality of life, with safety.展开更多
OBJECTIVE:To investigate the effects of Bazhen decoction(BZD),Siwu decoction(SWD) and Sijunzi decoction(SJZD) in mice with anemia induced by5-fluorouracil(5-FU) and discussed the possible pharmacological hematopoietic...OBJECTIVE:To investigate the effects of Bazhen decoction(BZD),Siwu decoction(SWD) and Sijunzi decoction(SJZD) in mice with anemia induced by5-fluorouracil(5-FU) and discussed the possible pharmacological hematopoietic mechanism to provide experimental evidence for the clinical use of the three classical prescriptions in the treatment of anemia.METHODS:Anemia was induced by intravenous injection of 5-FU and 80 female Kunming mice were randomly,assigned to oral administration of SWD,SJZD,or BZD daily for 10 days.Peripheral blood cells count and bone marrow cell cycle were monitored to evaluate anti-anemia effects.Serum cytokines,interferon-γ(IFN-γ),interleukin-3(IL-3),erythropoietin(EPO),granulocyte-macrophage colonystimulating factor(GM-CSF),and tumor necrosis factor-α(TNF-α) were assayed.EPO m RNA expression was assayed in kidney and liver tissue homogenates.RESULTS:BZD and SWD significantly increased the number of red blood cells,hemoglobin concentration,and hematocrit,promoted bone marrow cells to enter the cell cycle,proliferate and differentiate,significantly increased IL-3 secretion,and significantly inhibited IFN-γ secretion.BZD stimulated transcription of EPO m RNA in the kidney and liver and enhanced serum EPO expression.A therapeutic effect of SJZD was not observed.CONCLUSION:BZD and SWD treatment specifically enhanced hematopoietic function and mediated myelopoiesis by altering serum cytokines levels and accelerating entry of bone marrow cells into the cell cycle.Better curative effects were achieved via nourishing both Qi and blood(BZD) than by enriching the blood(SWD) or invigorating Qi(SWZD)alone.展开更多
Objective To identify the in vivo metabolized chemical constituents in Bazhen Decoction(BZD) and to study the relationship between Siwu Decoction(SWD) and Sijunzi Decoction(SJZD) with BZD.Methods Analysis and comparis...Objective To identify the in vivo metabolized chemical constituents in Bazhen Decoction(BZD) and to study the relationship between Siwu Decoction(SWD) and Sijunzi Decoction(SJZD) with BZD.Methods Analysis and comparison were carried out by HPLC-ESI-MS.Serum samples after ig administration of preparations such as SWD,SJZD,and BZD,with different ingredients were collected for analysis.Results Twenty-two components were detected after ig administration of BZD,and six of them were metabolites and others were original form of the components contained in BZD.The prototype constituents were atractylenolide I,5-hydroxymethyl-furoic acid(5-HMFA),oxypaeoniflorin,atractylenolide III,albiflorin,paeoniflorin,liquiritin,ferulic acid,ligustilide,and ginsenosides Rg1 and Rb1.The metabolized constituents were paeonimetabolin I,glycyrrhetic acid monoglucuronide,glycyrrhetinic acid,and ginsenosides Rh1 and Rd.Conclusion The results also show that 5-HMFA,oxypaeoniflorin,albiflorin,paeoniflorin,ferulic acid,and ligustilide are common components absorbed into blood existing in both BZD and SWD,which are material base of enriching blood;Atractylenolide I,atractylenolide III,and liquiritin are common ingredients absorbed into blood existing in both BZD and SJZD,and they are material base of invigorating Qi.The results provide basic data for the further studies on the effective components,the effecting mechanism,and the quality control of BZD.展开更多
文摘OBJECTIVE: To investigate the effects of Bazhen Pills on quality of life and immune function of patients undergoing chemotherapy after gallbladder cancer surgery. METHODS: A total of 64 patients with gallbladder cancer treated by surgery and postoperative basic nutritional support and symptomatic treatment in our hospital were selected and randomly divided into the control group and the observation group, with 32 cases in each group.The control group was treated with chemotherapy from 4^(th) to 6^(th) week after surgery. The observation group was treated by Bazhen Pills on the basis of the treatment in the control group. The blood cell count, immune function,TCM symptom scores, KPS scores, quality of life and adverse reactions were compared between the 2 groups.RESULTS: The WBC and PLT in 2 groups were decreased after the treatment, and the data in the observation group were higher than that in the control group(P < 0.05). The levels of CD3^+, CD4^+ and CD8^+ in the 2 groups were increased after the treatment(P < 0.05), and the data in the observation group were higher than that in the control group(P < 0.05). TCM syndrome scores of the 2 groups were decreased after the treatment(P < 0.05),and the KPS scores were increased after the treatment(P < 0.05). The improvement degrees of the 2 scores in the observation group were higher than that of the control group(P < 0.05). The fatigue symptom scores of the 2 groups were decreased while scores of physical function, role function and overall health were increased after the treatment(P < 0.05). The scores of the observation group were better than those of the control group(P < 0.05). The total incidence of grade I-II hematological toxicity and grade I-II gastrointestinal reactions in the observation group was lower than that in the control group(21.88% vs 50.00%)(P < 0.05). CONCLUSION: Bazhen Pills can effectively relieve clinical symptoms of patients undergoing chemotherapy after gallbladder cancer surgery, and improve their immune function and quality of life, with safety.
基金Supported by National Training Programs of Innovation for Undergraduates(No.201310635015)
文摘OBJECTIVE:To investigate the effects of Bazhen decoction(BZD),Siwu decoction(SWD) and Sijunzi decoction(SJZD) in mice with anemia induced by5-fluorouracil(5-FU) and discussed the possible pharmacological hematopoietic mechanism to provide experimental evidence for the clinical use of the three classical prescriptions in the treatment of anemia.METHODS:Anemia was induced by intravenous injection of 5-FU and 80 female Kunming mice were randomly,assigned to oral administration of SWD,SJZD,or BZD daily for 10 days.Peripheral blood cells count and bone marrow cell cycle were monitored to evaluate anti-anemia effects.Serum cytokines,interferon-γ(IFN-γ),interleukin-3(IL-3),erythropoietin(EPO),granulocyte-macrophage colonystimulating factor(GM-CSF),and tumor necrosis factor-α(TNF-α) were assayed.EPO m RNA expression was assayed in kidney and liver tissue homogenates.RESULTS:BZD and SWD significantly increased the number of red blood cells,hemoglobin concentration,and hematocrit,promoted bone marrow cells to enter the cell cycle,proliferate and differentiate,significantly increased IL-3 secretion,and significantly inhibited IFN-γ secretion.BZD stimulated transcription of EPO m RNA in the kidney and liver and enhanced serum EPO expression.A therapeutic effect of SJZD was not observed.CONCLUSION:BZD and SWD treatment specifically enhanced hematopoietic function and mediated myelopoiesis by altering serum cytokines levels and accelerating entry of bone marrow cells into the cell cycle.Better curative effects were achieved via nourishing both Qi and blood(BZD) than by enriching the blood(SWD) or invigorating Qi(SWZD)alone.
基金Postdoctoral Sustentation Fund of Heilongjiang Province of China (LBH-Q07060)
文摘Objective To identify the in vivo metabolized chemical constituents in Bazhen Decoction(BZD) and to study the relationship between Siwu Decoction(SWD) and Sijunzi Decoction(SJZD) with BZD.Methods Analysis and comparison were carried out by HPLC-ESI-MS.Serum samples after ig administration of preparations such as SWD,SJZD,and BZD,with different ingredients were collected for analysis.Results Twenty-two components were detected after ig administration of BZD,and six of them were metabolites and others were original form of the components contained in BZD.The prototype constituents were atractylenolide I,5-hydroxymethyl-furoic acid(5-HMFA),oxypaeoniflorin,atractylenolide III,albiflorin,paeoniflorin,liquiritin,ferulic acid,ligustilide,and ginsenosides Rg1 and Rb1.The metabolized constituents were paeonimetabolin I,glycyrrhetic acid monoglucuronide,glycyrrhetinic acid,and ginsenosides Rh1 and Rd.Conclusion The results also show that 5-HMFA,oxypaeoniflorin,albiflorin,paeoniflorin,ferulic acid,and ligustilide are common components absorbed into blood existing in both BZD and SWD,which are material base of enriching blood;Atractylenolide I,atractylenolide III,and liquiritin are common ingredients absorbed into blood existing in both BZD and SJZD,and they are material base of invigorating Qi.The results provide basic data for the further studies on the effective components,the effecting mechanism,and the quality control of BZD.