荧光原位杂交（fluorescence in situ hybridisation，FISH）是在石蜡包埋组织中检测细胞染色体变化及基因异常的一种敏感性强、特异性高的分子生物学方法。对石蜡包埋组织进行FISH染色所采用的方法有两种，在提取的细胞核上进行和直接...荧光原位杂交（fluorescence in situ hybridisation，FISH）是在石蜡包埋组织中检测细胞染色体变化及基因异常的一种敏感性强、特异性高的分子生物学方法。对石蜡包埋组织进行FISH染色所采用的方法有两种，在提取的细胞核上进行和直接在石蜡包埋组织切片上进行。然而，从石蜡包埋组织中提取细胞核进行FISH的方法复杂、耗时，而且所需组织较多，展开更多
Abstract:Objective To detect the mutation frequency of the bcl 10 gene in the early and advanced stages of hepatocellular carcinoma (HCC).Methods Genome DNA samples were extracted from 46 cases of fresh HCC tumor tiss...Abstract:Objective To detect the mutation frequency of the bcl 10 gene in the early and advanced stages of hepatocellular carcinoma (HCC).Methods Genome DNA samples were extracted from 46 cases of fresh HCC tumor tissues and their non-tumor adjacent tissues. Polymerase chain reaction-single strand conformation polymorphism method was used to detect point mutations of the three exons of the bcl 10 gene. For each individual exon, six random samples from those showing abnormal DNA bands were sequenced to verify those mutations. The relationship between serum alpha-fetoprotein (AFP) level and bcl 10 mutation, between the tumor size and bcl 10 mutation was also analyzed.Results Among the 46 samples, 26 cases (56.5%) were found to have mutations in exon 1, 5 out of the 6 cases were shown to have 5744 C→G mutation by sequencing; 25 cases (54.3%) were found to have mutations in exon 2, 4 out of the 6 cases were shown to have 11?311 T deletion mutation by sequencing. Twenty-one cases (45.7%) were found to have mutations in exon 3, all of the 6 cases selected for sequencing were shown to have 14?116 C→T mutation. Statistical analysis showed that neither serum alpha-fetoprotein level nor the size of hepatocellular carcinoma has a significant relationship with bcl 10 mutation.Conclusion The bcl 10 gene has a high mutation frequency in liver cancer.展开更多
文摘荧光原位杂交（fluorescence in situ hybridisation，FISH）是在石蜡包埋组织中检测细胞染色体变化及基因异常的一种敏感性强、特异性高的分子生物学方法。对石蜡包埋组织进行FISH染色所采用的方法有两种，在提取的细胞核上进行和直接在石蜡包埋组织切片上进行。然而，从石蜡包埋组织中提取细胞核进行FISH的方法复杂、耗时，而且所需组织较多，
文摘Abstract:Objective To detect the mutation frequency of the bcl 10 gene in the early and advanced stages of hepatocellular carcinoma (HCC).Methods Genome DNA samples were extracted from 46 cases of fresh HCC tumor tissues and their non-tumor adjacent tissues. Polymerase chain reaction-single strand conformation polymorphism method was used to detect point mutations of the three exons of the bcl 10 gene. For each individual exon, six random samples from those showing abnormal DNA bands were sequenced to verify those mutations. The relationship between serum alpha-fetoprotein (AFP) level and bcl 10 mutation, between the tumor size and bcl 10 mutation was also analyzed.Results Among the 46 samples, 26 cases (56.5%) were found to have mutations in exon 1, 5 out of the 6 cases were shown to have 5744 C→G mutation by sequencing; 25 cases (54.3%) were found to have mutations in exon 2, 4 out of the 6 cases were shown to have 11?311 T deletion mutation by sequencing. Twenty-one cases (45.7%) were found to have mutations in exon 3, all of the 6 cases selected for sequencing were shown to have 14?116 C→T mutation. Statistical analysis showed that neither serum alpha-fetoprotein level nor the size of hepatocellular carcinoma has a significant relationship with bcl 10 mutation.Conclusion The bcl 10 gene has a high mutation frequency in liver cancer.
