Multiplex ultrasensitive detection of low abundance proteins remains a significant challenge in clinical applications,necessitating the development of innovative solutions.The integration of bead-based microfluidic ch...Multiplex ultrasensitive detection of low abundance proteins remains a significant challenge in clinical applications,necessitating the development of innovative solutions.The integration of bead-based microfluidic chip platforms with their efficient target capture and separation capabilities,along with the advantages of miniaturization and low reagent consumption,holds great promise for building an integrated point-of-care testing(POCT)system that enables seamless sample input-result output.This review presents a comprehensive overview of recent advancements in bead-based microfluidic platforms for multiplex and ultrasensitive immunoassays,along with their potential applications in clinical diagnosis and treatment,which is organized into four sections:encoding techniques,the role of microfluidic platforms,applications,and future prospects.展开更多
基金supported by the National Natural Science Foundation of China[grant numbers 32001067,82272122,and 31927803]Inter-disciplinary Program of Shanghai Jiao Tong University[grant number YG2022ZD028]Major Projects of Special Development Funds for Shanghai Zhangjiang National Innovation Demonstration Zone[grant number ZJ2021-ZD-007].
文摘Multiplex ultrasensitive detection of low abundance proteins remains a significant challenge in clinical applications,necessitating the development of innovative solutions.The integration of bead-based microfluidic chip platforms with their efficient target capture and separation capabilities,along with the advantages of miniaturization and low reagent consumption,holds great promise for building an integrated point-of-care testing(POCT)system that enables seamless sample input-result output.This review presents a comprehensive overview of recent advancements in bead-based microfluidic platforms for multiplex and ultrasensitive immunoassays,along with their potential applications in clinical diagnosis and treatment,which is organized into four sections:encoding techniques,the role of microfluidic platforms,applications,and future prospects.
