In the work,bean callus raised from a leaves of Bean common mosaic virus infected bean plant was obtained and adapted for the testing of antiviral activity of liposomal glycan-glycolipid complexes.Ganoderma adspersum ...In the work,bean callus raised from a leaves of Bean common mosaic virus infected bean plant was obtained and adapted for the testing of antiviral activity of liposomal glycan-glycolipid complexes.Ganoderma adspersum glucans and Pseudomonas spec.rhamnolipids were constituents of liposomal compaunds.It has been shown that under the long-term cultivation(up to 3 months)in the presence of a liposomal preparation containing(10-100 mg/l),the virus is eliminated from the tissue.This is evidenced by the absence of 391 bp sequence amplification product established by RT-PCR in the callus tissue,cultured on a medium containing the liposomal complex.The proposed model system is analogous to plant tumors and has obvious advantages over similar systems in vivo,since the callus growth is controlled and independent of environmental factors.展开更多
The specific primers were designed according to conserved sequences of coat protein （CP） gene of Freesia mosaic virus （FreMV）, Cucumber mosaic vi- rus （CMV） and Bean yellow mosaic virus （BYMV） published in Gen...The specific primers were designed according to conserved sequences of coat protein （CP） gene of Freesia mosaic virus （FreMV）, Cucumber mosaic vi- rus （CMV） and Bean yellow mosaic virus （BYMV） published in GenBank, and a multiplex PCR protocol for simultaneous detection of these three viruses in freesia was developed. Three specific fragments were simultaneously amplified in a single PCR reaction. Their lengths were determined to be 340,628 and 212 bp, respec-tively. The sequence analysis indicated that three viruses shared at least 97% of homology with reference sequence. Sensitivity test showed that these three viruses could be detected out in the infected plant tissue greater than 10^-2 mg.展开更多
基金supplied by the National Academy of Sciences of Ukraine(governmental registration number of work is 0110U001871).
文摘In the work,bean callus raised from a leaves of Bean common mosaic virus infected bean plant was obtained and adapted for the testing of antiviral activity of liposomal glycan-glycolipid complexes.Ganoderma adspersum glucans and Pseudomonas spec.rhamnolipids were constituents of liposomal compaunds.It has been shown that under the long-term cultivation(up to 3 months)in the presence of a liposomal preparation containing(10-100 mg/l),the virus is eliminated from the tissue.This is evidenced by the absence of 391 bp sequence amplification product established by RT-PCR in the callus tissue,cultured on a medium containing the liposomal complex.The proposed model system is analogous to plant tumors and has obvious advantages over similar systems in vivo,since the callus growth is controlled and independent of environmental factors.
基金Supported by Major Projects of Science and Technology Plan of Fujian Province (2010NZ0003)Finance Special Project of Fujian Province "Project of Science and Technology Innovation Team Building of Fujian Academy of Agriculture" (CXTD2011-20)
文摘The specific primers were designed according to conserved sequences of coat protein （CP） gene of Freesia mosaic virus （FreMV）, Cucumber mosaic vi- rus （CMV） and Bean yellow mosaic virus （BYMV） published in GenBank, and a multiplex PCR protocol for simultaneous detection of these three viruses in freesia was developed. Three specific fragments were simultaneously amplified in a single PCR reaction. Their lengths were determined to be 340,628 and 212 bp, respec-tively. The sequence analysis indicated that three viruses shared at least 97% of homology with reference sequence. Sensitivity test showed that these three viruses could be detected out in the infected plant tissue greater than 10^-2 mg.
