In the work,bean callus raised from a leaves of Bean common mosaic virus infected bean plant was obtained and adapted for the testing of antiviral activity of liposomal glycan-glycolipid complexes.Ganoderma adspersum ...In the work,bean callus raised from a leaves of Bean common mosaic virus infected bean plant was obtained and adapted for the testing of antiviral activity of liposomal glycan-glycolipid complexes.Ganoderma adspersum glucans and Pseudomonas spec.rhamnolipids were constituents of liposomal compaunds.It has been shown that under the long-term cultivation(up to 3 months)in the presence of a liposomal preparation containing(10-100 mg/l),the virus is eliminated from the tissue.This is evidenced by the absence of 391 bp sequence amplification product established by RT-PCR in the callus tissue,cultured on a medium containing the liposomal complex.The proposed model system is analogous to plant tumors and has obvious advantages over similar systems in vivo,since the callus growth is controlled and independent of environmental factors.展开更多
根据哈尔滨地区豇豆感病植株的症状，初步鉴定感染豇豆的病毒为菜豆普通花叶病毒（Bean common mosaic， virus,SCMV）。利用马铃薯Y病毒属通用引物扩增出病毒基因组3’末端序列，经BLAST检索表明该病毒为BCMV，将该序列与GenBank上的21...根据哈尔滨地区豇豆感病植株的症状，初步鉴定感染豇豆的病毒为菜豆普通花叶病毒（Bean common mosaic， virus,SCMV）。利用马铃薯Y病毒属通用引物扩增出病毒基因组3’末端序列，经BLAST检索表明该病毒为BCMV，将该序列与GenBank上的21个BCMV分离物的3＇末端序列进行比较，显示其核苷酸序列与其他分离物的序列同源性为91.7％-97.3％。系统进化分析显示不同分离物可聚为5个类群，并显示出一定的寄主相关性。哈尔滨分离物BCMV-X与2个浙江分离物、1个澳大利亚分离物聚为一支，且该4株分离物的寄主均为豇豆。RNA二级结构分析显示BCMV基因组3’末端非编码区形成4个茎环结构，不同分离物的序列变化并未引起茎环结构的明显变化。展开更多
为确定从江苏省农科院蔬菜所温室大棚所采集到的表现花叶、皱缩、明脉症状的绿豆植株是由何种病毒引起的,采取了透射电子显微镜观察、RT-PCR、序列测序以及酶联免疫吸附法(ELISA)等方法进行鉴定。结果表明,在透射电镜下可观察到风轮状...为确定从江苏省农科院蔬菜所温室大棚所采集到的表现花叶、皱缩、明脉症状的绿豆植株是由何种病毒引起的,采取了透射电子显微镜观察、RT-PCR、序列测序以及酶联免疫吸附法(ELISA)等方法进行鉴定。结果表明,在透射电镜下可观察到风轮状、束状病毒内含体,与Potyvirus属病毒粒子形态一致;对所提取的病样总RNA使用Potyvirus通用引物进行RT-PCR扩增,得到由1 082个核苷酸组成的序列,包含编码228个氨基酸的完整cp基因,产物通过NCBI比对,发现和已报道的菜豆普通花叶病毒(Bean common mosaic virus,BCMV)HB分离物同源性高达95%,初步判定该病原为菜豆普通花叶病毒(BCMV),命名为BCMV-JAAS分离物;通过扩增得到的cp基因,构建系统发育树,表明BCMV-JAAS与BCMV的其他中国分离物亲缘关系较近;通过ELISA检测,进一步确定是由菜豆普通花叶病毒(BCMV)感染引起的绿豆病毒病,这是我国第一次在绿豆上发现菜豆普通花叶病毒。展开更多
在四个菜豆品种“沙克沙”、“家雀蛋”、“自来油”、“白大架”上按种菜豆花叶病毒(Bean Common Mosaic Virus(BCMV)V-5],接种后15—25天测定,病毒在植株体内的浓度达到高峰。同时,植高、叶面积,叶绿素总量,光合速率较健康株明显下降...在四个菜豆品种“沙克沙”、“家雀蛋”、“自来油”、“白大架”上按种菜豆花叶病毒(Bean Common Mosaic Virus(BCMV)V-5],接种后15—25天测定,病毒在植株体内的浓度达到高峰。同时,植高、叶面积,叶绿素总量,光合速率较健康株明显下降,呼吸强度、游离态氨基酸总量上升。接种后30—35天,病毒含量开始下降,光合速率,呼吸强度、株高几乎接近正常水平。叶面积、叶绿素总量,游离态氨基酸总量同健株的差异也大大减少。继病毒侵入后植株生理代谢的失调,温室盆栽条件下,菜豆不同品种的产量损失为40—64％。展开更多
基金supplied by the National Academy of Sciences of Ukraine(governmental registration number of work is 0110U001871).
文摘In the work,bean callus raised from a leaves of Bean common mosaic virus infected bean plant was obtained and adapted for the testing of antiviral activity of liposomal glycan-glycolipid complexes.Ganoderma adspersum glucans and Pseudomonas spec.rhamnolipids were constituents of liposomal compaunds.It has been shown that under the long-term cultivation(up to 3 months)in the presence of a liposomal preparation containing(10-100 mg/l),the virus is eliminated from the tissue.This is evidenced by the absence of 391 bp sequence amplification product established by RT-PCR in the callus tissue,cultured on a medium containing the liposomal complex.The proposed model system is analogous to plant tumors and has obvious advantages over similar systems in vivo,since the callus growth is controlled and independent of environmental factors.
文摘根据哈尔滨地区豇豆感病植株的症状，初步鉴定感染豇豆的病毒为菜豆普通花叶病毒（Bean common mosaic， virus,SCMV）。利用马铃薯Y病毒属通用引物扩增出病毒基因组3’末端序列，经BLAST检索表明该病毒为BCMV，将该序列与GenBank上的21个BCMV分离物的3＇末端序列进行比较，显示其核苷酸序列与其他分离物的序列同源性为91.7％-97.3％。系统进化分析显示不同分离物可聚为5个类群，并显示出一定的寄主相关性。哈尔滨分离物BCMV-X与2个浙江分离物、1个澳大利亚分离物聚为一支，且该4株分离物的寄主均为豇豆。RNA二级结构分析显示BCMV基因组3’末端非编码区形成4个茎环结构，不同分离物的序列变化并未引起茎环结构的明显变化。
文摘为确定从江苏省农科院蔬菜所温室大棚所采集到的表现花叶、皱缩、明脉症状的绿豆植株是由何种病毒引起的,采取了透射电子显微镜观察、RT-PCR、序列测序以及酶联免疫吸附法(ELISA)等方法进行鉴定。结果表明,在透射电镜下可观察到风轮状、束状病毒内含体,与Potyvirus属病毒粒子形态一致;对所提取的病样总RNA使用Potyvirus通用引物进行RT-PCR扩增,得到由1 082个核苷酸组成的序列,包含编码228个氨基酸的完整cp基因,产物通过NCBI比对,发现和已报道的菜豆普通花叶病毒(Bean common mosaic virus,BCMV)HB分离物同源性高达95%,初步判定该病原为菜豆普通花叶病毒(BCMV),命名为BCMV-JAAS分离物;通过扩增得到的cp基因,构建系统发育树,表明BCMV-JAAS与BCMV的其他中国分离物亲缘关系较近;通过ELISA检测,进一步确定是由菜豆普通花叶病毒(BCMV)感染引起的绿豆病毒病,这是我国第一次在绿豆上发现菜豆普通花叶病毒。
文摘在四个菜豆品种“沙克沙”、“家雀蛋”、“自来油”、“白大架”上按种菜豆花叶病毒(Bean Common Mosaic Virus(BCMV)V-5],接种后15—25天测定,病毒在植株体内的浓度达到高峰。同时,植高、叶面积,叶绿素总量,光合速率较健康株明显下降,呼吸强度、游离态氨基酸总量上升。接种后30—35天,病毒含量开始下降,光合速率,呼吸强度、株高几乎接近正常水平。叶面积、叶绿素总量,游离态氨基酸总量同健株的差异也大大减少。继病毒侵入后植株生理代谢的失调,温室盆栽条件下,菜豆不同品种的产量损失为40—64％。
