The specific primers were designed according to conserved sequences of coat protein （CP） gene of Freesia mosaic virus （FreMV）, Cucumber mosaic vi- rus （CMV） and Bean yellow mosaic virus （BYMV） published in Gen...The specific primers were designed according to conserved sequences of coat protein （CP） gene of Freesia mosaic virus （FreMV）, Cucumber mosaic vi- rus （CMV） and Bean yellow mosaic virus （BYMV） published in GenBank, and a multiplex PCR protocol for simultaneous detection of these three viruses in freesia was developed. Three specific fragments were simultaneously amplified in a single PCR reaction. Their lengths were determined to be 340,628 and 212 bp, respec-tively. The sequence analysis indicated that three viruses shared at least 97% of homology with reference sequence. Sensitivity test showed that these three viruses could be detected out in the infected plant tissue greater than 10^-2 mg.展开更多
基金Supported by Major Projects of Science and Technology Plan of Fujian Province (2010NZ0003)Finance Special Project of Fujian Province "Project of Science and Technology Innovation Team Building of Fujian Academy of Agriculture" (CXTD2011-20)
文摘The specific primers were designed according to conserved sequences of coat protein （CP） gene of Freesia mosaic virus （FreMV）, Cucumber mosaic vi- rus （CMV） and Bean yellow mosaic virus （BYMV） published in GenBank, and a multiplex PCR protocol for simultaneous detection of these three viruses in freesia was developed. Three specific fragments were simultaneously amplified in a single PCR reaction. Their lengths were determined to be 340,628 and 212 bp, respec-tively. The sequence analysis indicated that three viruses shared at least 97% of homology with reference sequence. Sensitivity test showed that these three viruses could be detected out in the infected plant tissue greater than 10^-2 mg.
