Rho-related GTPase from plants(ROP)proteins play an essential role in plant stress resistance.In this study,the full-length GlROP6 gene was cloned based on G.littoralis transcriptome sequencing data acquired in respon...Rho-related GTPase from plants(ROP)proteins play an essential role in plant stress resistance.In this study,the full-length GlROP6 gene was cloned based on G.littoralis transcriptome sequencing data acquired in response to salt stress.The protein sequence,conserved domains,secondary structure,three-dimensional structure,phylogenetic relationships,and expression pattern of the GlROP6 gene were systematically analysed.Our results showed that the full-length GlROP6 gene had an open reading frame of 606 bp,which encoded 201 amino acid residues with a relative molecular weight of 22.23463 kDa and a theoretical isoelectric point of 9.06.Amino acid sequence analyses indicated that the structure of the GlROP6 protein was conserved,and included five G-box motifs(G1–G5),an effector binding region,a Rho insert region and a C-terminal hypervariable region.According to our phylogenetic analysis,the GlROP6 protein was closely related to the ROP protein of Daucus carota subsp.Sativus.Our quantitative real-time PCR results revealed that GlROP6 was highly expressed in flower,and GlROP6 expression was significantly upregulated in G.littoralis roots treated with NaCl.This study will facilitate investigations into the function of GlROP genes in response to salt stress in G.littoralis.展开更多
More and more attention has been paid to the polysaccharide components of Glehnia littoralis.It has good immunoregulation,antioxidant,antitumor,hypoglycemic and other biological activities,as well as special health ca...More and more attention has been paid to the polysaccharide components of Glehnia littoralis.It has good immunoregulation,antioxidant,antitumor,hypoglycemic and other biological activities,as well as special health care functions.Therefore,it has a broad application prospect in medical,health care,food and other industries.In this paper,the extraction,purification,content determination and pharmacological activities of polysaccharides from Glehnia littoralis were reviewed,which provided a gauge for the further development and utilization of Glehnia littoralis.展开更多
为探究珊瑚菜(Glehnia littoralis F.Schmidt ex Miq.)叶绿体基因组密码子使用偏性及其影响因子,本研究基于珊瑚菜叶绿体基因组测序数据筛选到51个蛋白质编码基因,并对其密码子使用模式进行分析。结果显示:珊瑚菜叶绿体基因组密码子总G...为探究珊瑚菜(Glehnia littoralis F.Schmidt ex Miq.)叶绿体基因组密码子使用偏性及其影响因子,本研究基于珊瑚菜叶绿体基因组测序数据筛选到51个蛋白质编码基因,并对其密码子使用模式进行分析。结果显示:珊瑚菜叶绿体基因组密码子总GC含量(G_(Call))为38.8%,密码子第3位碱基的GC含量(GC3)最低,仅为29.2%,3个位置碱基的GC含量均小于50%,表明珊瑚菜叶绿体基因组密码子偏好使用A或U。珊瑚菜叶绿体基因组有效密码子数(ENC)为34.6~54.5,密码子适应指数(CAI)为0.100~0.270,密码子偏好指数(CBI)为-0.190~0.226,最优密码子使用频率(FOP)为0.276~0.550,表明珊瑚菜叶绿体基因组密码子使用偏性较弱;相关性分析结果表明珊瑚菜叶绿体基因组密码子第3位碱基的组成对基因表达有很大影响。中性绘图分析、PR2-plot分析、ENC-plot分析和对应性分析结果表明珊瑚菜叶绿体基因组密码子使用偏性受自然选择和突变的共同影响,以自然选择为主;基于相对同义密码子使用度(RSCU)和高、低表达组的RSCU差值(ΔRSCU)共获得16个最优密码子,且大部分以A或U结尾。综合分析结果表明:珊瑚菜叶绿体基因组密码子使用偏性较弱,且主要受自然选择的影响。展开更多
珊瑚菜(Glehnia littoralis Fr.Schmidt ex Miq.)又名北沙参,为伞形科珊瑚菜属的单种属植物,主要生长在沿海地区的海滨沙滩上,根可入药。由于城市的扩张、旅游景点的设立及港口的建设等原因,珊瑚菜的生存环境遭到破坏,加之人为的掠夺性...珊瑚菜(Glehnia littoralis Fr.Schmidt ex Miq.)又名北沙参,为伞形科珊瑚菜属的单种属植物,主要生长在沿海地区的海滨沙滩上,根可入药。由于城市的扩张、旅游景点的设立及港口的建设等原因,珊瑚菜的生存环境遭到破坏,加之人为的掠夺性采挖,其分布及资源逐渐减少,已被列为国家Ⅱ级重点保护野生植物。在辽宁省主要分布区内展开了对珊瑚菜野生资源的调查,结果显示:仅在大连长海一处面积约100 m^(2)的海滩发现一个珊瑚菜极小种群,该种群植株数量不足100株,呈聚集性分布,且以幼年植株为主,能开花结实的数量有限;在绥中的珊瑚菜原生境保护点中未发现珊瑚菜植株。根据调查结果得出结论:野生珊瑚菜目前在辽宁已经极度濒危,应加强保护。展开更多
Using silica gel column chromatography and recrystallization,a light yellow needle crystal with a purity of 97.21% was obtained from ethyl acetate fraction of ethanol extract from dry root of Glehnia littoralis Fr.Sch...Using silica gel column chromatography and recrystallization,a light yellow needle crystal with a purity of 97.21% was obtained from ethyl acetate fraction of ethanol extract from dry root of Glehnia littoralis Fr.Schmidt ex Miq.,and was identified as bergapten by means of NMR.The experimental result of antitumor activity in vitro shows that 0.01-100.00 mg·L-1 bergapten have inhibitory effect to liver cancer cell line HEP-G2,in which inhibition of 100.00 mg·L-1 bergapten is the highest with an inhibitory rate of 95.0%.In concentrations of 0.01-100.00 mg·L-1,only 100.00 mg·L-1 bergapten has an inhibitory effect to stomach cancer cell line SGC-7901 with an inhibitory rate of 79.6%.展开更多
为了进一步研究药材北沙参(Glehnia littoralisF.Schmidt ex Miq)的药效物质基础,采用硅胶柱色谱、凝胶色谱Sephadex LH-20和高效液相色谱方法,从药材北沙参中分离得到了8个化合物。运用IR、1DNMR、2DNMR及与文献比较,鉴定它们的结构分...为了进一步研究药材北沙参(Glehnia littoralisF.Schmidt ex Miq)的药效物质基础,采用硅胶柱色谱、凝胶色谱Sephadex LH-20和高效液相色谱方法,从药材北沙参中分离得到了8个化合物。运用IR、1DNMR、2DNMR及与文献比较,鉴定它们的结构分别为:羽扇豆醇(Ⅰ)、桦木醇(Ⅱ)、β-谷甾醇(Ⅲ)、胡萝卜苷(Ⅳ)、人参炔醇(Ⅴ)、法卡林二醇(Ⅵ)、(8E)-1,8-heptadecadiene-4,6-diyne-3,10-diol(Ⅶ)和异欧前胡素(Ⅷ)。其中,化合物Ⅰ,Ⅱ和Ⅳ为首次从该药材中分离得到。展开更多
基金supported by grants from the National Science Foundation of the Jiangsu Higher Education institutions of China(No.18KJB180002)the National Natural Science Foundation of China(No.31800272)。
文摘Rho-related GTPase from plants(ROP)proteins play an essential role in plant stress resistance.In this study,the full-length GlROP6 gene was cloned based on G.littoralis transcriptome sequencing data acquired in response to salt stress.The protein sequence,conserved domains,secondary structure,three-dimensional structure,phylogenetic relationships,and expression pattern of the GlROP6 gene were systematically analysed.Our results showed that the full-length GlROP6 gene had an open reading frame of 606 bp,which encoded 201 amino acid residues with a relative molecular weight of 22.23463 kDa and a theoretical isoelectric point of 9.06.Amino acid sequence analyses indicated that the structure of the GlROP6 protein was conserved,and included five G-box motifs(G1–G5),an effector binding region,a Rho insert region and a C-terminal hypervariable region.According to our phylogenetic analysis,the GlROP6 protein was closely related to the ROP protein of Daucus carota subsp.Sativus.Our quantitative real-time PCR results revealed that GlROP6 was highly expressed in flower,and GlROP6 expression was significantly upregulated in G.littoralis roots treated with NaCl.This study will facilitate investigations into the function of GlROP genes in response to salt stress in G.littoralis.
文摘More and more attention has been paid to the polysaccharide components of Glehnia littoralis.It has good immunoregulation,antioxidant,antitumor,hypoglycemic and other biological activities,as well as special health care functions.Therefore,it has a broad application prospect in medical,health care,food and other industries.In this paper,the extraction,purification,content determination and pharmacological activities of polysaccharides from Glehnia littoralis were reviewed,which provided a gauge for the further development and utilization of Glehnia littoralis.
文摘为探究珊瑚菜(Glehnia littoralis F.Schmidt ex Miq.)叶绿体基因组密码子使用偏性及其影响因子,本研究基于珊瑚菜叶绿体基因组测序数据筛选到51个蛋白质编码基因,并对其密码子使用模式进行分析。结果显示:珊瑚菜叶绿体基因组密码子总GC含量(G_(Call))为38.8%,密码子第3位碱基的GC含量(GC3)最低,仅为29.2%,3个位置碱基的GC含量均小于50%,表明珊瑚菜叶绿体基因组密码子偏好使用A或U。珊瑚菜叶绿体基因组有效密码子数(ENC)为34.6~54.5,密码子适应指数(CAI)为0.100~0.270,密码子偏好指数(CBI)为-0.190~0.226,最优密码子使用频率(FOP)为0.276~0.550,表明珊瑚菜叶绿体基因组密码子使用偏性较弱;相关性分析结果表明珊瑚菜叶绿体基因组密码子第3位碱基的组成对基因表达有很大影响。中性绘图分析、PR2-plot分析、ENC-plot分析和对应性分析结果表明珊瑚菜叶绿体基因组密码子使用偏性受自然选择和突变的共同影响,以自然选择为主;基于相对同义密码子使用度(RSCU)和高、低表达组的RSCU差值(ΔRSCU)共获得16个最优密码子,且大部分以A或U结尾。综合分析结果表明:珊瑚菜叶绿体基因组密码子使用偏性较弱,且主要受自然选择的影响。
文摘Using silica gel column chromatography and recrystallization,a light yellow needle crystal with a purity of 97.21% was obtained from ethyl acetate fraction of ethanol extract from dry root of Glehnia littoralis Fr.Schmidt ex Miq.,and was identified as bergapten by means of NMR.The experimental result of antitumor activity in vitro shows that 0.01-100.00 mg·L-1 bergapten have inhibitory effect to liver cancer cell line HEP-G2,in which inhibition of 100.00 mg·L-1 bergapten is the highest with an inhibitory rate of 95.0%.In concentrations of 0.01-100.00 mg·L-1,only 100.00 mg·L-1 bergapten has an inhibitory effect to stomach cancer cell line SGC-7901 with an inhibitory rate of 79.6%.
文摘为了进一步研究药材北沙参(Glehnia littoralisF.Schmidt ex Miq)的药效物质基础,采用硅胶柱色谱、凝胶色谱Sephadex LH-20和高效液相色谱方法,从药材北沙参中分离得到了8个化合物。运用IR、1DNMR、2DNMR及与文献比较,鉴定它们的结构分别为:羽扇豆醇(Ⅰ)、桦木醇(Ⅱ)、β-谷甾醇(Ⅲ)、胡萝卜苷(Ⅳ)、人参炔醇(Ⅴ)、法卡林二醇(Ⅵ)、(8E)-1,8-heptadecadiene-4,6-diyne-3,10-diol(Ⅶ)和异欧前胡素(Ⅷ)。其中,化合物Ⅰ,Ⅱ和Ⅳ为首次从该药材中分离得到。