Molluscan metabolomic analysis is essential for the understanding of the regulatory mechanism of aquatic invertebrate in response to hepatotoxic microcystins(MCs)stress.To understand the system responses of the gastro...Molluscan metabolomic analysis is essential for the understanding of the regulatory mechanism of aquatic invertebrate in response to hepatotoxic microcystins(MCs)stress.To understand the system responses of the gastropod to MC exposure,metabolomic alterations caused by two strains(MC-producing and non MC-producing)of Microcystis aeruginosa were characterized indiff erent biological matrices(hepatopancreas and muscle)of Bellamya aeruginosa(Gastropoda)using 1 H nuclear magnetic resonance(NMR)spectroscopy combined with MCs detections after exposure for 1,7,and 14 d.Although ELISA analysis showed that no MCs was detected in both tissues after non MC-producing M.aeruginosa exposure,MCs concentrations were increasing in the hepatopancreas(from 1.29±0.48μg/g to 3.17±0.11μg/g)and foot muscle(from 0.07±0.02μg/g to 0.21±0.08μg/g)after 14-d exposure of MC-producing M.aeruginosa.Meanwhile,we observed that MC induced signifi cant increase in creatine,a variety of amino acids(leucine,isoleucine,valine,threonine,alanine,methionine,glutamate,aspartate,and lysine),carboxylic acids(lactate,acetate,and D-3-hydroxybutyrate),and choline and its derivatives(phosphocholine and glycerophosphocholine)but decreased the energy substance(lipids,glucose,and glycogen)in the hepatopancreas.However,no signifi cant metabolite diff erences were observed in the muscle between MC-producing and non MC-producing cyanobacteria treated groups.These results suggest that MC exposure may cause hepatic energy expenditure accompanied with various metabolic disorders that involve lipid metabolism,protein catabolism,osmoregulation,glycolysis,glycogenolysis,and tricarboxylic acid(TCA)cycle.Moreover,metabolic perturbation was aggravated as the level of accumulated MCs raised over time in the MC-producing cyanobacteria treatment.These fi ndings indicated that MCs accumulation might lead to oxidative-stress-mediated damage of mitochondria functions.展开更多
15N isotope tracer techniques and ecological modeling were adopted to investigate the fractionation of nitrogen,its uptake and transformation in algae and snail(Bellamya aeruginosa Reeve).Different algal species were ...15N isotope tracer techniques and ecological modeling were adopted to investigate the fractionation of nitrogen,its uptake and transformation in algae and snail(Bellamya aeruginosa Reeve).Different algal species were found to differ in their uptake of nitrogen isotopes.Microcystis aeruginisa Ktz.demonstrated the greatest 15N accumulation capacity,with the natural variation in isotopic ratio(δ 15N) and the isotope fractionation factor(ε,‰) being the highest among the species investigated.The transformation and utilization of 15N by snails differed depending on the specific algae consumed(highest for Chlorella pyrenoidosa Chick.,lowest for M.aeruginisa).When snails was seeded in the experimental pond,the algae population structure changed significantly,and total algal biomass as well as the concentration of all nitrogen species decreased,causing an increase in water transparency.A model,incorporating several chemical and biological parameters,was developed to predict algal biomass in an aquatic system when snails was present.The data collected during this investigation indicated that the gastropods such as snails could significantly impact biological community and water quality of small water bodies,suggesting a role for biological control of noxious algal blooms associated with eutrophication.展开更多
The energy budget of Bellamya earuginosa in a shallow algal lake, Houhu Lake (Wuhan, China) was investigated by the measurement of flesh production (32.8kJ/(m 2·a)), egestion (337.7 kJ/(m 2·a)), metabolism (...The energy budget of Bellamya earuginosa in a shallow algal lake, Houhu Lake (Wuhan, China) was investigated by the measurement of flesh production (32.8kJ/(m 2·a)), egestion (337.7 kJ/(m 2·a)), metabolism (246.7 kJ/(m 2·a)), and estimation of excretion (21.4kJ/(m 2·a)). The net growth efficiency of the species is about 10.9%, which accords with the generally reported value for gastropods. In addition, the relationships between starvation respiration ( R , mgO 2/(Ind·d)), body weight ( Wd , mg in dry wt) and temperature ( T , ℃) were also determined. The regression equation R =0.044 Wd 0.537 e 0.061T was obtained by the least square method, The measured SDA of the species is 26.51% of its gross metabolism.展开更多
基金Supported by the National Natural Science Foundation of China(Nos.42077219,31302192)the Natural Science Foundation of Zhejiang Province(No.LQ20C190003)+2 种基金the Ningbo Municipal Natural Science Foundation(No.2019A610421)the Ningbo Public Welfare Technology Application Research Project(No.202002N3049)the Hangzhou Municipal Agriculture and Social Development Project(No.2020ZDSJ0697),the Fundamental Research Funds for the Provincial Universities of Zhejiang(No.SJLY2020011),and the K.C.Wong Magna Fund in Ningbo University。
文摘Molluscan metabolomic analysis is essential for the understanding of the regulatory mechanism of aquatic invertebrate in response to hepatotoxic microcystins(MCs)stress.To understand the system responses of the gastropod to MC exposure,metabolomic alterations caused by two strains(MC-producing and non MC-producing)of Microcystis aeruginosa were characterized indiff erent biological matrices(hepatopancreas and muscle)of Bellamya aeruginosa(Gastropoda)using 1 H nuclear magnetic resonance(NMR)spectroscopy combined with MCs detections after exposure for 1,7,and 14 d.Although ELISA analysis showed that no MCs was detected in both tissues after non MC-producing M.aeruginosa exposure,MCs concentrations were increasing in the hepatopancreas(from 1.29±0.48μg/g to 3.17±0.11μg/g)and foot muscle(from 0.07±0.02μg/g to 0.21±0.08μg/g)after 14-d exposure of MC-producing M.aeruginosa.Meanwhile,we observed that MC induced signifi cant increase in creatine,a variety of amino acids(leucine,isoleucine,valine,threonine,alanine,methionine,glutamate,aspartate,and lysine),carboxylic acids(lactate,acetate,and D-3-hydroxybutyrate),and choline and its derivatives(phosphocholine and glycerophosphocholine)but decreased the energy substance(lipids,glucose,and glycogen)in the hepatopancreas.However,no signifi cant metabolite diff erences were observed in the muscle between MC-producing and non MC-producing cyanobacteria treated groups.These results suggest that MC exposure may cause hepatic energy expenditure accompanied with various metabolic disorders that involve lipid metabolism,protein catabolism,osmoregulation,glycolysis,glycogenolysis,and tricarboxylic acid(TCA)cycle.Moreover,metabolic perturbation was aggravated as the level of accumulated MCs raised over time in the MC-producing cyanobacteria treatment.These fi ndings indicated that MCs accumulation might lead to oxidative-stress-mediated damage of mitochondria functions.
基金supported by the National Natural Science Foundation of China (No.30870452)the National Chaohu Lake Special Project of China (No.2008ZX07103-005)
文摘15N isotope tracer techniques and ecological modeling were adopted to investigate the fractionation of nitrogen,its uptake and transformation in algae and snail(Bellamya aeruginosa Reeve).Different algal species were found to differ in their uptake of nitrogen isotopes.Microcystis aeruginisa Ktz.demonstrated the greatest 15N accumulation capacity,with the natural variation in isotopic ratio(δ 15N) and the isotope fractionation factor(ε,‰) being the highest among the species investigated.The transformation and utilization of 15N by snails differed depending on the specific algae consumed(highest for Chlorella pyrenoidosa Chick.,lowest for M.aeruginisa).When snails was seeded in the experimental pond,the algae population structure changed significantly,and total algal biomass as well as the concentration of all nitrogen species decreased,causing an increase in water transparency.A model,incorporating several chemical and biological parameters,was developed to predict algal biomass in an aquatic system when snails was present.The data collected during this investigation indicated that the gastropods such as snails could significantly impact biological community and water quality of small water bodies,suggesting a role for biological control of noxious algal blooms associated with eutrophication.
文摘The energy budget of Bellamya earuginosa in a shallow algal lake, Houhu Lake (Wuhan, China) was investigated by the measurement of flesh production (32.8kJ/(m 2·a)), egestion (337.7 kJ/(m 2·a)), metabolism (246.7 kJ/(m 2·a)), and estimation of excretion (21.4kJ/(m 2·a)). The net growth efficiency of the species is about 10.9%, which accords with the generally reported value for gastropods. In addition, the relationships between starvation respiration ( R , mgO 2/(Ind·d)), body weight ( Wd , mg in dry wt) and temperature ( T , ℃) were also determined. The regression equation R =0.044 Wd 0.537 e 0.061T was obtained by the least square method, The measured SDA of the species is 26.51% of its gross metabolism.
