The role of human papillomavirus in breast cancer pathogenesis is an area of considerable debate following differing results from all over the world. This study analyzed paraffin embedded tissue from 19 patients with ...The role of human papillomavirus in breast cancer pathogenesis is an area of considerable debate following differing results from all over the world. This study analyzed paraffin embedded tissue from 19 patients with benign or malignant breast disease. Standard PCR with GP5+/6+ and FAP primers, proved negative in all cases. However, qRT-PCR using the SF10 primer set detected HPV DNA in 2 out of these samples. Whilst our data does not support a major role for HPV in the pathogenesis of breast disease in Malta, it definitely does not exclude it totally. This study also clearly shows that the method of analysis is one of the variables resulting in the range of differing results witnessed worldwide in this area of study.展开更多
Background: The difficulty with histopathology diagnosis is the presence of numerous benign reactive breast lesions with morphological features mimic malignant lesions. Thus, the objective of this study was to assess ...Background: The difficulty with histopathology diagnosis is the presence of numerous benign reactive breast lesions with morphological features mimic malignant lesions. Thus, the objective of this study was to assess the merit of morphological patterns of elastic and reticular fibers in differential diagnosis of benign and malignant breast lesions. Methodology: Fifty biopsies were obtained from females with breast Lesions (25 breast carcinoma and 25 benign lesions), their ages ranging from 17 to 85 years with mean age of 39 years old. Morphologic demonstrations of elastic and reticular fibers were performed using conventional histochemical procedures. Results: Intense grades of elastic fiber staining were achieved with carcinoma tissues. Dwindled grades of elastic fiber staining were detected with fibrocystic changes. Elastic fibers in breast carcinoma are significantly increased compared to benign breast lesions P Conclusion: Histochemical quantifications of elastic and reticular fibers can assist in routine diagnosis of breast lesions. Elastic fibers significantly increase in breast carcinoma compared to benign breast lesions.展开更多
Objective: To describe the radiological and histological aspects of breast nodules among men in our practice setting. Methodology: This is a descriptive and retrospective study conducted from January 2014 to October 2...Objective: To describe the radiological and histological aspects of breast nodules among men in our practice setting. Methodology: This is a descriptive and retrospective study conducted from January 2014 to October 2017. The study included male patients with breast nodules classified ACR3, 4 or 5 after a mammographic and ultrasound scan. These lesions were explored through an ultrasound-guided breast microbiopsy. The following are the variables collected: age, family history of breast cancer, medical history, ACR classification, nodule size, and anatomopathologic diagnosis. Results: 13 breast nodules were explored among 13 male subjects. Mammography and breast ultrasound enabled to identify one ACR3 lesion, 10 ACR4 lesions and 2 ACR5 lesions. The average age was 56.53 ± 14.63 years and the average size of the nodules 28.91 ± 13.62 mm. As for histology, there were seven malignant tumors and six benign tumors. The average age of patients with malignancy was 61.45 ± 13.62 years. The malignant nodules average size was 29.45 ± 12.54 mm. Malignant tumors exclusively consisted of invasive breast carcinomas while Benign ones consisted of dystrophic and inflammatory lesions. Conclusion: Most often Breast lesions among men had an indeterminate appearance on imaging and were predominantly malignant in histopathology.展开更多
目的研究乳腺癌肿瘤组织脾酪氨酸激酶(SYK)联合母体胚胎亮氨酸拉链激酶(MELK)检测在乳腺癌病情及预后评估中的临床价值。方法选择2019年1月至2021年12月常德市第一人民诊治的95例乳腺癌患者(乳腺癌组)和51例乳腺良性疾病患者(对照组)为...目的研究乳腺癌肿瘤组织脾酪氨酸激酶(SYK)联合母体胚胎亮氨酸拉链激酶(MELK)检测在乳腺癌病情及预后评估中的临床价值。方法选择2019年1月至2021年12月常德市第一人民诊治的95例乳腺癌患者(乳腺癌组)和51例乳腺良性疾病患者(对照组)为研究对象。实时荧光定量PCR检测SYK、MELK表达并比较在不同临床病理特征中的差异。ROC曲线分析肿瘤组织SYK、MELK表达在乳腺癌预后不良预测中的敏感度、特异度及AUC。多因素Logistics回归分析乳腺癌预后不良的独立危险因素。Kaplan-Meier生存模型及Log rank比较不同肿瘤组织SYK、MELK表达中生存期的差异。结果乳腺癌组SYK表达显著低于对照组(0.54±0.08 vs 0.71±0.12),MELK表达显著高于对照组(0.87±0.11 vs 0.45±0.07),差异有统计学意义(P<0.05)。乳腺癌患者肿瘤组织中SYK、MELK表达与年龄、肿瘤部位无关(P>0.05),与组织学分级、T分期、转移淋巴结数、远传转移及TNM分期有关(P<0.05)。SYK联合MELK在乳腺癌患者预后不良预测中的敏感度、特异度及AUC均高于SYK、MELK、组织学分级、T分期、转移淋巴结数、远处转移、TNM分期(P<0.05)。SYK≤0.54、MELK≥0.87、组织学分级3级、T分期T3~T4、转移淋巴结数N2~N3、远处转移M1、TNM分期Ⅲ~Ⅳ期为乳腺癌预后不良的独立危险因素(P<0.05)。肿瘤组织SYK≤0.54且MELK≥0.87乳腺癌患者中位生存期显著低于SYK﹥0.54或MELK﹤0.87患者[中位生存期(32.37±4.01)个月vs(38.65±5.19)个月,Log rank=15.127,P<0.01]。结论乳腺癌患者SYK表达显著降低而MELK表达显著增高,与临床病理特征及生存期存在关联,在乳腺癌病情及预后中具有较高临床价值。SYK及MELK联合检测可显著提高预测乳腺癌预后不良的临床价值。展开更多
目的调查乳腺疾病患者营养状况的现状,探讨患者体质量与乳腺癌发生的关系,为预防和筛选乳腺癌提供循证依据。方法便利抽样法选取2012年6-11月在上海交通大学医学院附属瑞金医院乳腺疾病诊治中心住院治疗的1187例患者为研究对象,采用营...目的调查乳腺疾病患者营养状况的现状,探讨患者体质量与乳腺癌发生的关系,为预防和筛选乳腺癌提供循证依据。方法便利抽样法选取2012年6-11月在上海交通大学医学院附属瑞金医院乳腺疾病诊治中心住院治疗的1187例患者为研究对象,采用营养风险筛查工具(nutritional risk screening-2002,NRS-2002)对患者的营养状况进行调查,记录其实验室检查指标,并计算体质量指数(body mass index,BMI);随访记录患者住院天数、住院费用及乳腺癌患者的临床分期。结果1187例乳腺疾病患者中有乳腺癌676例(56.95%)和乳腺良性疾病511例(43.05%)。乳腺良性疾病患者中有营养风险患者的比例要高于乳腺癌患者,差异有统计学意义(P<0.05)。乳腺癌者中体质量超过正常者的比例要高于乳腺良性疾病患者,差异有统计学意义(P<0.05)。乳腺癌患者的白蛋白水平、淋巴细胞计数明显低于乳腺良性疾病患者,而乳腺癌患者的前白蛋白水平却高于乳腺良性疾病患者,差异均有统计学意义(均P<0.05)。乳腺癌患者中以临床Ⅰ期(33.38%)和Ⅱ期(53.06%)患者占多数;不同BMI组患者在其临床分期上的差异有统计学意义(P<0.05)。结论乳腺癌患者中体质量高于正常者占多数,且超重或肥胖与乳腺癌的发生有一定联系。展开更多
文摘The role of human papillomavirus in breast cancer pathogenesis is an area of considerable debate following differing results from all over the world. This study analyzed paraffin embedded tissue from 19 patients with benign or malignant breast disease. Standard PCR with GP5+/6+ and FAP primers, proved negative in all cases. However, qRT-PCR using the SF10 primer set detected HPV DNA in 2 out of these samples. Whilst our data does not support a major role for HPV in the pathogenesis of breast disease in Malta, it definitely does not exclude it totally. This study also clearly shows that the method of analysis is one of the variables resulting in the range of differing results witnessed worldwide in this area of study.
文摘Background: The difficulty with histopathology diagnosis is the presence of numerous benign reactive breast lesions with morphological features mimic malignant lesions. Thus, the objective of this study was to assess the merit of morphological patterns of elastic and reticular fibers in differential diagnosis of benign and malignant breast lesions. Methodology: Fifty biopsies were obtained from females with breast Lesions (25 breast carcinoma and 25 benign lesions), their ages ranging from 17 to 85 years with mean age of 39 years old. Morphologic demonstrations of elastic and reticular fibers were performed using conventional histochemical procedures. Results: Intense grades of elastic fiber staining were achieved with carcinoma tissues. Dwindled grades of elastic fiber staining were detected with fibrocystic changes. Elastic fibers in breast carcinoma are significantly increased compared to benign breast lesions P Conclusion: Histochemical quantifications of elastic and reticular fibers can assist in routine diagnosis of breast lesions. Elastic fibers significantly increase in breast carcinoma compared to benign breast lesions.
文摘Objective: To describe the radiological and histological aspects of breast nodules among men in our practice setting. Methodology: This is a descriptive and retrospective study conducted from January 2014 to October 2017. The study included male patients with breast nodules classified ACR3, 4 or 5 after a mammographic and ultrasound scan. These lesions were explored through an ultrasound-guided breast microbiopsy. The following are the variables collected: age, family history of breast cancer, medical history, ACR classification, nodule size, and anatomopathologic diagnosis. Results: 13 breast nodules were explored among 13 male subjects. Mammography and breast ultrasound enabled to identify one ACR3 lesion, 10 ACR4 lesions and 2 ACR5 lesions. The average age was 56.53 ± 14.63 years and the average size of the nodules 28.91 ± 13.62 mm. As for histology, there were seven malignant tumors and six benign tumors. The average age of patients with malignancy was 61.45 ± 13.62 years. The malignant nodules average size was 29.45 ± 12.54 mm. Malignant tumors exclusively consisted of invasive breast carcinomas while Benign ones consisted of dystrophic and inflammatory lesions. Conclusion: Most often Breast lesions among men had an indeterminate appearance on imaging and were predominantly malignant in histopathology.
文摘目的研究乳腺癌肿瘤组织脾酪氨酸激酶(SYK)联合母体胚胎亮氨酸拉链激酶(MELK)检测在乳腺癌病情及预后评估中的临床价值。方法选择2019年1月至2021年12月常德市第一人民诊治的95例乳腺癌患者(乳腺癌组)和51例乳腺良性疾病患者(对照组)为研究对象。实时荧光定量PCR检测SYK、MELK表达并比较在不同临床病理特征中的差异。ROC曲线分析肿瘤组织SYK、MELK表达在乳腺癌预后不良预测中的敏感度、特异度及AUC。多因素Logistics回归分析乳腺癌预后不良的独立危险因素。Kaplan-Meier生存模型及Log rank比较不同肿瘤组织SYK、MELK表达中生存期的差异。结果乳腺癌组SYK表达显著低于对照组(0.54±0.08 vs 0.71±0.12),MELK表达显著高于对照组(0.87±0.11 vs 0.45±0.07),差异有统计学意义(P<0.05)。乳腺癌患者肿瘤组织中SYK、MELK表达与年龄、肿瘤部位无关(P>0.05),与组织学分级、T分期、转移淋巴结数、远传转移及TNM分期有关(P<0.05)。SYK联合MELK在乳腺癌患者预后不良预测中的敏感度、特异度及AUC均高于SYK、MELK、组织学分级、T分期、转移淋巴结数、远处转移、TNM分期(P<0.05)。SYK≤0.54、MELK≥0.87、组织学分级3级、T分期T3~T4、转移淋巴结数N2~N3、远处转移M1、TNM分期Ⅲ~Ⅳ期为乳腺癌预后不良的独立危险因素(P<0.05)。肿瘤组织SYK≤0.54且MELK≥0.87乳腺癌患者中位生存期显著低于SYK﹥0.54或MELK﹤0.87患者[中位生存期(32.37±4.01)个月vs(38.65±5.19)个月,Log rank=15.127,P<0.01]。结论乳腺癌患者SYK表达显著降低而MELK表达显著增高,与临床病理特征及生存期存在关联,在乳腺癌病情及预后中具有较高临床价值。SYK及MELK联合检测可显著提高预测乳腺癌预后不良的临床价值。
文摘目的调查乳腺疾病患者营养状况的现状,探讨患者体质量与乳腺癌发生的关系,为预防和筛选乳腺癌提供循证依据。方法便利抽样法选取2012年6-11月在上海交通大学医学院附属瑞金医院乳腺疾病诊治中心住院治疗的1187例患者为研究对象,采用营养风险筛查工具(nutritional risk screening-2002,NRS-2002)对患者的营养状况进行调查,记录其实验室检查指标,并计算体质量指数(body mass index,BMI);随访记录患者住院天数、住院费用及乳腺癌患者的临床分期。结果1187例乳腺疾病患者中有乳腺癌676例(56.95%)和乳腺良性疾病511例(43.05%)。乳腺良性疾病患者中有营养风险患者的比例要高于乳腺癌患者,差异有统计学意义(P<0.05)。乳腺癌者中体质量超过正常者的比例要高于乳腺良性疾病患者,差异有统计学意义(P<0.05)。乳腺癌患者的白蛋白水平、淋巴细胞计数明显低于乳腺良性疾病患者,而乳腺癌患者的前白蛋白水平却高于乳腺良性疾病患者,差异均有统计学意义(均P<0.05)。乳腺癌患者中以临床Ⅰ期(33.38%)和Ⅱ期(53.06%)患者占多数;不同BMI组患者在其临床分期上的差异有统计学意义(P<0.05)。结论乳腺癌患者中体质量高于正常者占多数,且超重或肥胖与乳腺癌的发生有一定联系。
