Rice cultivar Norin 8 and its mutant Norin 8m harbour bentazon resistance trait and bentazon susceptibility trait respectively. A total of 360 arbitrary 10-mer oligonucleotide primers were screened on the genomic DNA ...Rice cultivar Norin 8 and its mutant Norin 8m harbour bentazon resistance trait and bentazon susceptibility trait respectively. A total of 360 arbitrary 10-mer oligonucleotide primers were screened on the genomic DNA of Norin 8 and Norin 8m with RAPD technique. Among which, five primers produced seven polymorphic RAPD bands between Norin 8 and Norin 8m. Amplified RAPD polymorphic products were cloned and sequenced. The sequences were used to design primers for PCR. Five SCAR markers, SCAR/G18/883, SCAR/G18/890, SCAR/G18/919/948, SCAR/D10/1237 and SCAR/F03/1186, were developed from OPG18/943, OPG18/972, OPD10/1248 and OPF03/1198. F-2 progeny of 320 individuals was analyzed to map SCAR markers in relationship to ben or Ben genes. SCAR markers of SCAR/G18/883, SCAR/G18/890, SCAR/G18/919/948 were shown to cosegregate with ben or Ben genes, and SCAR/D10/1237 to be linked of Ben gene with a distance of (14.8 +/- 2.1) cM. The genetic linkage to ben gene and SCAR markers was identified by a pair of near isogenic lines H121 and Hben121. Southern blotting analysis and segregation ratio of F-2 progeny revealed that OPG18/943 and OPG18/972 were single-copy in genome, and locus of OPG18/943 and OPG18/972 were allelic and sequence tagged sites. It is the first report on molecular markers linked to ben or Ben genes. The markers are useful to marker-assisted selection for the breeding and tag ben gene with map-based cloning.展开更多
The seed production of male and female parent of ‘Hunzhi 1＇ contained bentazon susceptible gene was studied. The result showed that spraying 3. 375 L/hm2 48% bentazon aqua could control the self-pollinated fertiliza...The seed production of male and female parent of ‘Hunzhi 1＇ contained bentazon susceptible gene was studied. The result showed that spraying 3. 375 L/hm2 48% bentazon aqua could control the self-pollinated fertilization rate below 1.0% and survival plant rate was 0. The spray of bentazon would not influence MC526 pollen vitality. Seed setting rate of female parent was not obvious different from that of control group with spraying bentazon aqua more than 3. 375 L/hm^2 from the sprouting appearing period to full heading stage. This demonstrated that bentazon aqua had no influence on seed setting rate during florescence of female parent. The weight ratio between male parent seeds and female parent seeds was 1 to 5, then seeds from parents with that weight ratio would be mixedly planted for seed production. With this weight ratio, relative proper structure of panicle and grain would be obtained ,panicle ratio was 1 to 4.3, glumous flowers ratio was 1 to 3.5. Cross seed-setting rate of female parent was 72.6% and seed production per hm^2 was 66 000 kg.展开更多
The application of a mixture of bentazone(3-isopropyl-1H-2,1,3-benzothiadiazin-4(3H)-one-2,2-dioxide)and atrazine (6-chloro-N2-ethyl-N4-isopropyl-1,3,5-triazine-2,4-diamine)is a practical approach to enhance the herbi...The application of a mixture of bentazone(3-isopropyl-1H-2,1,3-benzothiadiazin-4(3H)-one-2,2-dioxide)and atrazine (6-chloro-N2-ethyl-N4-isopropyl-1,3,5-triazine-2,4-diamine)is a practical approach to enhance the herbicidal effect.Labo- ratory incubation experiments were performed to study the degradation of bentazone and atrazine applied in combination and individually in maize rhizosphere and non-rhizosphere soils.After a lag phase,the degradation of each individual herbicide in the non-autoclaved soil could be adequately described using a first-order kinetic equation.During a 30-d in- cubation,in the autoclaved rhizosphere soil,bentazone and atrazine did not noticeably degrade,but in the non-autoclaved soil,they rapidly degraded in both non-rhizosphere and rhizosphere soils with half-lives of 19.9 and 20.2 d for bentazone and 29.1 and 25.7 d for atrazine,respectively.The rhizosphere effect significantly enhanced the degradation of atrazine, but had no significant effect on bentazone.These results indicated that biological degradation accounted for the degrada- tion of both herbicides in the soil.When compared with the degradation of the herbicide applied alone,the degradation rates of the herbicides applied in combination in the soils were lower and the lag phase increased.With the addition of a surfactant,Tween-20,a reduced lag phase of degradation was observed for both herbicides applied in combination. The degradation rate of bentazone accelerated,whereas that of atrazine remained nearly unchanged.Thus,when these two herbicides were used simultaneously,their persistence in the soil was generally prolonged,and the environmental contamination potential increased.展开更多
In progenies resulting from crosses involving rice cultivar Norin 8m susceptible to bentazon as the donor of ben gene, SCARs tightly linked to ben were utilized for selection of ben. The homozygous and heterozygous ge...In progenies resulting from crosses involving rice cultivar Norin 8m susceptible to bentazon as the donor of ben gene, SCARs tightly linked to ben were utilized for selection of ben. The homozygous and heterozygous genotypes with ben could be identified with the SCARs. The molecular markers offer a powerful tool for indirect selection of ben and can accelerate the introgression of ben into current rice cultivars.展开更多
New herbicide options are needed for postemergence (POST) broadleaf weed control in adzuki bean. A field study, of five experiments, was conducted over a three-year period (2014, 2015, 2016) in Ontario to evaluate the...New herbicide options are needed for postemergence (POST) broadleaf weed control in adzuki bean. A field study, of five experiments, was conducted over a three-year period (2014, 2015, 2016) in Ontario to evaluate the tolerance of adzuki bean to the POST application of acifluorfen (600 and 1200 g·ai·ha-1), fomesafen (240 and 480 g·ai·ha-1), bentazon (1080 and 2160 g·ai·ha-1), imazethapyr (100 and 200 g·ai·ha-1) and halosulfuron-methyl (75 and 150 g·ai·ha-1). Acifluorfen and fomesafen applied POST caused as much as 12% visible injury at the 1X rate and 20% visible injury at the 2X rate but had no adverse effect on adzuki bean population, shoot dry weight, height, maturity or yield. Bentazon caused as much as 23% visible injury at 1080 g·ai·ha-1 and 28% visible injury at 2160 g·ai·ha-1 but caused no adverse effect on adzuki bean population, shoot dry weight, height, maturity or yield at either rate, except at 2160 g·ai·ha-1 which reduced shoot dry weight 20% and height 12%. Imazethapyr caused as much as 22% visible injury at 100 g·ai·ha-1 and 34% visible injury at 200 g·ai·ha-1 but caused no adverse effect on adzuki bean population, shoot dry weight, height, maturity or yield at either rate except at 200 g·ai·ha-1 which delayed maturity slightly. Halosulfuron-methyl caused as much as 65% visible injury and reduced shoot dry weight, height and yield 64%, 41%, and 28%, respectively. This research concludes that acfluorfen, fomesafen, bentazon, imazethapyr and halosulfuron at the rates evaluated can cause the significant injury in adzuki bean.展开更多
Bentazon, applied as a tankmix, has been shown to have the potential for reducing the injury from some POST herbicides. Field experiments were conducted in 2008 and 2009 at Exeter, ON and in 2009 at Ridgetown, ON to d...Bentazon, applied as a tankmix, has been shown to have the potential for reducing the injury from some POST herbicides. Field experiments were conducted in 2008 and 2009 at Exeter, ON and in 2009 at Ridgetown, ON to determine if the addition of bentazon reduces the injury from cloransulam-methyl or halosulfuron-methyl applied POST in black, cranberry, kidney and white beans. Bentazon added to cloransulam-methyl reduced the level of injury 0 to 6% at 17.5 g·ai·ha–1 and 0 to 9% at 35 g·ai·ha–1 in dry bean. Bentazon added to halosulfuron-methyl reduced the level of injury as much as 4% at 35 g·ai·ha–1 and 6% at the 70 g·ai·ha–1. Bentazon added to cloransulam-methyl increased plant height as much as 3 cm. The addition of bentazon to halosulfuron-methyl had no effect on the height of various market classes of dry bean. Bentazon added to cloran-sulam-methyl generally has no effect on seed moisture content in black and white bean but decreased seed moisture content of cranberry and kidney bean as much as 4%. The addition of bentazon to halosulfuron-methyl caused no effect on seed moisture content of dry bean. Cloransulam-methyl caused a 7% to 18% reduction in dry bean yield compared to halosulfuron-methyl and 12% to 21% reduction in yield compared to bentazon. Bentazon added to cloransulam-methyl increased dry bean yield by 0.16 and 0.31 t·ha–1 at Exeter (2009) and Ridgetown (2009) respectively. The addition of bentazon to halosulfuron-methyl had no effect on dry bean yield.展开更多
The contamination of surface and ground water by bentazone has attracted increasing global concern in recent years. We conducted a detailed investigation using MIEX resin to eliminate bentazone from waters. Batch expe...The contamination of surface and ground water by bentazone has attracted increasing global concern in recent years. We conducted a detailed investigation using MIEX resin to eliminate bentazone from waters. Batch experiments were carried out to evaluate the effect of process parameters, such as retention time, resin amount, and initial pesticide concentration, on removal efficiency of bentazone. Results showed the sorption process was fast and bentazone could be efficiently removed in 30 minutes. The kinetic process of bentazone sorption on MIEX resin was well described by pseudo second-order model and intraparticle diffusion was the rate controlling step. The MIEX resin possessed the highest sorption capacity of 0.2656 mmol/mL for bentazone according to Langmuir fitting, Bentazone is a hydrophobic ionizable organic compound, and both ionic charge and hydrophobic aromatic structure governed the sorption characteristics on MIEX resin. The different removal efficiencies of ionic and non-ionic pesticides, combined with the charge balance equations of bentazone, SO4^2-, NO3- and Cl-, indicated that removal of bentazone using MIEX resin occurred primarily via ion exchange.展开更多
Groundwater contamination due to pesticide applications on agricultural lands is of great environmental concern. The mathematical models help to understand the mechanism of pesticide leaching in soils towards groundwa...Groundwater contamination due to pesticide applications on agricultural lands is of great environmental concern. The mathematical models help to understand the mechanism of pesticide leaching in soils towards groundwater. We developed a user-friendly model called ArcPRZM-3 by integrating widely used Pesticide Root Zone Model version 3 （PRZM-3） using Visual Basic and Geographic Information System （GIS） based Avenue programming. ArcPRZM-3 could be used to simulate pesticide leaching towards groundwater with user-friendly input interfaces coupled with databases of crops, soils and pesticides. The outputs from ArcPRZM-3 could be visualized in user-friendly formats of tables, charts and maps. In this study we evaluated ArcPRZM-3 model by simulating bentazon leaching in soil towards groundwater. ArcPRZM-3 was applied to 37 sites in Woodruff County, Arkansas, USA to observe the daily average dissolved bentazon concentration for soybean, sorghum and rice at a depth of 1.8 m for a period of two years. Nineteen ranks of bentazon leaching potential were obtained using ArcPRZM-3 for all sites having different soil and crop combinations. ArcPRZM-3 simulation results for bentazon were compatible with the field monitored data in term of relative ranking and trend, although some uncertainties exist. This study indicated that macropore flow mechanism would be important in analyzing the effect of irrigation on groundwater contamination due to pesticides. Overall, ArcPRZM-3 could be used to simulate pesticide leaching towards groundwater more efficiently and effectively as compared to PRZM-3.展开更多
Hybrid rice planting has been widely popularized and applied in the world. However, the high cost of seed production and the complicated procedures have become a bottleneck in the development of hybrid rice. The resea...Hybrid rice planting has been widely popularized and applied in the world. However, the high cost of seed production and the complicated procedures have become a bottleneck in the development of hybrid rice. The research progress on mixed sowing seed production techniques of hybrid rice was introduced from the aspects of rice resources creation, breeding, sowing seed technology research and cost benefit analysis. The production technology of the new mixed seeding combina- tion ＂Xinhunyou 6＂ was investigated, including the research and validation of benta- zon treatment period and dosage, mixing ratio of male and female parents, and the comparative test of different different sowing methods, which revealed that the mechanization technology of seed production of hybrid rice was mature and feasible and would be one of the most important development trend of technological devel- opment of hybrid rice production.展开更多
文摘Rice cultivar Norin 8 and its mutant Norin 8m harbour bentazon resistance trait and bentazon susceptibility trait respectively. A total of 360 arbitrary 10-mer oligonucleotide primers were screened on the genomic DNA of Norin 8 and Norin 8m with RAPD technique. Among which, five primers produced seven polymorphic RAPD bands between Norin 8 and Norin 8m. Amplified RAPD polymorphic products were cloned and sequenced. The sequences were used to design primers for PCR. Five SCAR markers, SCAR/G18/883, SCAR/G18/890, SCAR/G18/919/948, SCAR/D10/1237 and SCAR/F03/1186, were developed from OPG18/943, OPG18/972, OPD10/1248 and OPF03/1198. F-2 progeny of 320 individuals was analyzed to map SCAR markers in relationship to ben or Ben genes. SCAR markers of SCAR/G18/883, SCAR/G18/890, SCAR/G18/919/948 were shown to cosegregate with ben or Ben genes, and SCAR/D10/1237 to be linked of Ben gene with a distance of (14.8 +/- 2.1) cM. The genetic linkage to ben gene and SCAR markers was identified by a pair of near isogenic lines H121 and Hben121. Southern blotting analysis and segregation ratio of F-2 progeny revealed that OPG18/943 and OPG18/972 were single-copy in genome, and locus of OPG18/943 and OPG18/972 were allelic and sequence tagged sites. It is the first report on molecular markers linked to ben or Ben genes. The markers are useful to marker-assisted selection for the breeding and tag ben gene with map-based cloning.
基金Supported by the Important Science and Technology Key Project of An-hui Eleventh-five Year Plan(06003010B)the International Cooperation Program of Anhui Province(06088013)~~
文摘The seed production of male and female parent of ‘Hunzhi 1＇ contained bentazon susceptible gene was studied. The result showed that spraying 3. 375 L/hm2 48% bentazon aqua could control the self-pollinated fertilization rate below 1.0% and survival plant rate was 0. The spray of bentazon would not influence MC526 pollen vitality. Seed setting rate of female parent was not obvious different from that of control group with spraying bentazon aqua more than 3. 375 L/hm^2 from the sprouting appearing period to full heading stage. This demonstrated that bentazon aqua had no influence on seed setting rate during florescence of female parent. The weight ratio between male parent seeds and female parent seeds was 1 to 5, then seeds from parents with that weight ratio would be mixedly planted for seed production. With this weight ratio, relative proper structure of panicle and grain would be obtained ,panicle ratio was 1 to 4.3, glumous flowers ratio was 1 to 3.5. Cross seed-setting rate of female parent was 72.6% and seed production per hm^2 was 66 000 kg.
基金the Natural Science Basic Research Plan in Shaanxi Province of China(No.2004K03-G3)the Scientific Research Fund of the Department of Education of Shaanxi ProvinceChina(No.04JK234)
文摘The application of a mixture of bentazone(3-isopropyl-1H-2,1,3-benzothiadiazin-4(3H)-one-2,2-dioxide)and atrazine (6-chloro-N2-ethyl-N4-isopropyl-1,3,5-triazine-2,4-diamine)is a practical approach to enhance the herbicidal effect.Labo- ratory incubation experiments were performed to study the degradation of bentazone and atrazine applied in combination and individually in maize rhizosphere and non-rhizosphere soils.After a lag phase,the degradation of each individual herbicide in the non-autoclaved soil could be adequately described using a first-order kinetic equation.During a 30-d in- cubation,in the autoclaved rhizosphere soil,bentazone and atrazine did not noticeably degrade,but in the non-autoclaved soil,they rapidly degraded in both non-rhizosphere and rhizosphere soils with half-lives of 19.9 and 20.2 d for bentazone and 29.1 and 25.7 d for atrazine,respectively.The rhizosphere effect significantly enhanced the degradation of atrazine, but had no significant effect on bentazone.These results indicated that biological degradation accounted for the degrada- tion of both herbicides in the soil.When compared with the degradation of the herbicide applied alone,the degradation rates of the herbicides applied in combination in the soils were lower and the lag phase increased.With the addition of a surfactant,Tween-20,a reduced lag phase of degradation was observed for both herbicides applied in combination. The degradation rate of bentazone accelerated,whereas that of atrazine remained nearly unchanged.Thus,when these two herbicides were used simultaneously,their persistence in the soil was generally prolonged,and the environmental contamination potential increased.
基金This work was supported by grants from Anhui Province Natura1 Science Foundation(0004111O).
文摘In progenies resulting from crosses involving rice cultivar Norin 8m susceptible to bentazon as the donor of ben gene, SCARs tightly linked to ben were utilized for selection of ben. The homozygous and heterozygous genotypes with ben could be identified with the SCARs. The molecular markers offer a powerful tool for indirect selection of ben and can accelerate the introgression of ben into current rice cultivars.
文摘New herbicide options are needed for postemergence (POST) broadleaf weed control in adzuki bean. A field study, of five experiments, was conducted over a three-year period (2014, 2015, 2016) in Ontario to evaluate the tolerance of adzuki bean to the POST application of acifluorfen (600 and 1200 g·ai·ha-1), fomesafen (240 and 480 g·ai·ha-1), bentazon (1080 and 2160 g·ai·ha-1), imazethapyr (100 and 200 g·ai·ha-1) and halosulfuron-methyl (75 and 150 g·ai·ha-1). Acifluorfen and fomesafen applied POST caused as much as 12% visible injury at the 1X rate and 20% visible injury at the 2X rate but had no adverse effect on adzuki bean population, shoot dry weight, height, maturity or yield. Bentazon caused as much as 23% visible injury at 1080 g·ai·ha-1 and 28% visible injury at 2160 g·ai·ha-1 but caused no adverse effect on adzuki bean population, shoot dry weight, height, maturity or yield at either rate, except at 2160 g·ai·ha-1 which reduced shoot dry weight 20% and height 12%. Imazethapyr caused as much as 22% visible injury at 100 g·ai·ha-1 and 34% visible injury at 200 g·ai·ha-1 but caused no adverse effect on adzuki bean population, shoot dry weight, height, maturity or yield at either rate except at 200 g·ai·ha-1 which delayed maturity slightly. Halosulfuron-methyl caused as much as 65% visible injury and reduced shoot dry weight, height and yield 64%, 41%, and 28%, respectively. This research concludes that acfluorfen, fomesafen, bentazon, imazethapyr and halosulfuron at the rates evaluated can cause the significant injury in adzuki bean.
文摘Bentazon, applied as a tankmix, has been shown to have the potential for reducing the injury from some POST herbicides. Field experiments were conducted in 2008 and 2009 at Exeter, ON and in 2009 at Ridgetown, ON to determine if the addition of bentazon reduces the injury from cloransulam-methyl or halosulfuron-methyl applied POST in black, cranberry, kidney and white beans. Bentazon added to cloransulam-methyl reduced the level of injury 0 to 6% at 17.5 g·ai·ha–1 and 0 to 9% at 35 g·ai·ha–1 in dry bean. Bentazon added to halosulfuron-methyl reduced the level of injury as much as 4% at 35 g·ai·ha–1 and 6% at the 70 g·ai·ha–1. Bentazon added to cloransulam-methyl increased plant height as much as 3 cm. The addition of bentazon to halosulfuron-methyl had no effect on the height of various market classes of dry bean. Bentazon added to cloran-sulam-methyl generally has no effect on seed moisture content in black and white bean but decreased seed moisture content of cranberry and kidney bean as much as 4%. The addition of bentazon to halosulfuron-methyl caused no effect on seed moisture content of dry bean. Cloransulam-methyl caused a 7% to 18% reduction in dry bean yield compared to halosulfuron-methyl and 12% to 21% reduction in yield compared to bentazon. Bentazon added to cloransulam-methyl increased dry bean yield by 0.16 and 0.31 t·ha–1 at Exeter (2009) and Ridgetown (2009) respectively. The addition of bentazon to halosulfuron-methyl had no effect on dry bean yield.
基金supported by the National High-Tech Research and Development Program (863) of China(No. 2008AA06A414)the National Natural Science Foundation of China (No. 50921064)
文摘The contamination of surface and ground water by bentazone has attracted increasing global concern in recent years. We conducted a detailed investigation using MIEX resin to eliminate bentazone from waters. Batch experiments were carried out to evaluate the effect of process parameters, such as retention time, resin amount, and initial pesticide concentration, on removal efficiency of bentazone. Results showed the sorption process was fast and bentazone could be efficiently removed in 30 minutes. The kinetic process of bentazone sorption on MIEX resin was well described by pseudo second-order model and intraparticle diffusion was the rate controlling step. The MIEX resin possessed the highest sorption capacity of 0.2656 mmol/mL for bentazone according to Langmuir fitting, Bentazone is a hydrophobic ionizable organic compound, and both ionic charge and hydrophobic aromatic structure governed the sorption characteristics on MIEX resin. The different removal efficiencies of ionic and non-ionic pesticides, combined with the charge balance equations of bentazone, SO4^2-, NO3- and Cl-, indicated that removal of bentazone using MIEX resin occurred primarily via ion exchange.
基金supported by the United States Department of Agriculture Cooperative State Research,Education,and Extension Service(USDA-CSREES) grant through a subcontract from the University of Arkansas
文摘Groundwater contamination due to pesticide applications on agricultural lands is of great environmental concern. The mathematical models help to understand the mechanism of pesticide leaching in soils towards groundwater. We developed a user-friendly model called ArcPRZM-3 by integrating widely used Pesticide Root Zone Model version 3 （PRZM-3） using Visual Basic and Geographic Information System （GIS） based Avenue programming. ArcPRZM-3 could be used to simulate pesticide leaching towards groundwater with user-friendly input interfaces coupled with databases of crops, soils and pesticides. The outputs from ArcPRZM-3 could be visualized in user-friendly formats of tables, charts and maps. In this study we evaluated ArcPRZM-3 model by simulating bentazon leaching in soil towards groundwater. ArcPRZM-3 was applied to 37 sites in Woodruff County, Arkansas, USA to observe the daily average dissolved bentazon concentration for soybean, sorghum and rice at a depth of 1.8 m for a period of two years. Nineteen ranks of bentazon leaching potential were obtained using ArcPRZM-3 for all sites having different soil and crop combinations. ArcPRZM-3 simulation results for bentazon were compatible with the field monitored data in term of relative ranking and trend, although some uncertainties exist. This study indicated that macropore flow mechanism would be important in analyzing the effect of irrigation on groundwater contamination due to pesticides. Overall, ArcPRZM-3 could be used to simulate pesticide leaching towards groundwater more efficiently and effectively as compared to PRZM-3.
基金Supported by the National High-tech R&D Program(863 Program)(2014AA10A603)the Key Science and Technology Program during the"13thFive Year Plan"of Anhui Province(1604a0702008)+1 种基金the Special Fund for Agro-scientific Research in the Public Interest(201503130)the Project for the Team of Science and Technology Innovation of Anhui Academy of Agricultural Sciences(15C0108)~~
文摘Hybrid rice planting has been widely popularized and applied in the world. However, the high cost of seed production and the complicated procedures have become a bottleneck in the development of hybrid rice. The research progress on mixed sowing seed production techniques of hybrid rice was introduced from the aspects of rice resources creation, breeding, sowing seed technology research and cost benefit analysis. The production technology of the new mixed seeding combina- tion ＂Xinhunyou 6＂ was investigated, including the research and validation of benta- zon treatment period and dosage, mixing ratio of male and female parents, and the comparative test of different different sowing methods, which revealed that the mechanization technology of seed production of hybrid rice was mature and feasible and would be one of the most important development trend of technological devel- opment of hybrid rice production.
