Objective To identify the active compounds from the barks of Betula platyphylla for inhibitory on diacylglycerol acyltransferase（DGAT1）.Methods Bioassay-guided fractionation resulted in the isolation of DGAT1 inhibi...Objective To identify the active compounds from the barks of Betula platyphylla for inhibitory on diacylglycerol acyltransferase（DGAT1）.Methods Bioassay-guided fractionation resulted in the isolation of DGAT1 inhibitory activity of lupane triterpenes.Results Ten compounds were identified as lupenone（1）,lupeol（2）,betulinic acid（3）,betulinaldehyde（4）,betulin（5）,3-deoxybetulonic acid（6）,glochidonol（7）,lup-20/29-ene-1β/3β-diol（8）,3α-hydroxy-lup-20（29）-en-23,28-dioic acid（9）,and 3α,11α-dihydroxy-23-oxo-lup-20（29）-en-28-oic acid（10）.Compounds 3-6,9,and 10 inhibited DGAT1 with IC50 values ranging from（11.2±0.3）to（38.6±1.2）μmol/L.Conclusion Compounds 6,9,and 10 are first isolated from the barks of B.platyphylla.,and compounds 3-6,9,and 10 from the barks of B.platyphylla are responsible for the inhibition on DGAT1.展开更多
基金Science and Technology Development Program of Jilin Province(201205099)
文摘Objective To identify the active compounds from the barks of Betula platyphylla for inhibitory on diacylglycerol acyltransferase（DGAT1）.Methods Bioassay-guided fractionation resulted in the isolation of DGAT1 inhibitory activity of lupane triterpenes.Results Ten compounds were identified as lupenone（1）,lupeol（2）,betulinic acid（3）,betulinaldehyde（4）,betulin（5）,3-deoxybetulonic acid（6）,glochidonol（7）,lup-20/29-ene-1β/3β-diol（8）,3α-hydroxy-lup-20（29）-en-23,28-dioic acid（9）,and 3α,11α-dihydroxy-23-oxo-lup-20（29）-en-28-oic acid（10）.Compounds 3-6,9,and 10 inhibited DGAT1 with IC50 values ranging from（11.2±0.3）to（38.6±1.2）μmol/L.Conclusion Compounds 6,9,and 10 are first isolated from the barks of B.platyphylla.,and compounds 3-6,9,and 10 from the barks of B.platyphylla are responsible for the inhibition on DGAT1.
