The bio-sandstone, which was cemented by microbe cement, was firstly prepared, and then the microstructure evolution process was studied by X-ray computed tomography （X-CT） technique. The experimental results indica...The bio-sandstone, which was cemented by microbe cement, was firstly prepared, and then the microstructure evolution process was studied by X-ray computed tomography （X-CT） technique. The experimental results indicate that the microstructure of bio-sandstone becomes dense with the development of age. The evolution of inner structure at different positions is different due to the different contents of microbial induced precipitation calcite. Besides, the increase rate of microbial induced precipitation calcite gradually decreases because of the reduction of microbe absorption content with the decreasing pore size in bio-sandstone.展开更多
Bacillus subtilis was selected as the suitable microorganism,which could produce alkaline phosphatase and constantly hydrolyzed phosphate monoester in the mixture solution of bacteria with substrate,and then the PO4^3...Bacillus subtilis was selected as the suitable microorganism,which could produce alkaline phosphatase and constantly hydrolyzed phosphate monoester in the mixture solution of bacteria with substrate,and then the PO4^3-was obtained.Bio-phosphate cement was prepared by alkaline earth element（Ba）ions reacting with PO4^3-in the mixture solution.Structure,size and thermal properties of the bio-phosphate cement were characterized by energy dispersive X-ray spectroscopy（EDS）,X-ray diffraction（XRD）,scanning electron microscopy（SEM）,and particle size analysis.The average crystallite sizes of chem-BaHPO4 and bio-BaHPO4corresponded to 11.99 and 24.13μm,respectively.Chem-BaHPO4 and bio-BaHPO4 were then adopted to bind loose sand particles.The results indicated that loose sand particles can be well cemented by the bio-BaHPO4powder into a bio-sandstone with a certain mechanical properties,and the average compressive strength of the bio-sandstones can be up to 0.83 MPa when the curing time was 14 d.Along with the method in future studies,there will be multiple new opportunities for engineering applications,for instance,the treatment of sandy soil foundation,remediation of heavy metals in contaminated soil,and so on.展开更多
基金Funded by the National Natural Science Foundation of China(No.51072035),the Ph D Program’s Foundation of Ministry of Education of China(No.20090092110029)the Research Innovation Program for College Graduates of Jiangsu Province(No.CXZZ_0145)the Scientific Research Foundation of Graduate School of Southeast University(Nos.YBJJ1127 and YBPY1208)
文摘The bio-sandstone, which was cemented by microbe cement, was firstly prepared, and then the microstructure evolution process was studied by X-ray computed tomography （X-CT） technique. The experimental results indicate that the microstructure of bio-sandstone becomes dense with the development of age. The evolution of inner structure at different positions is different due to the different contents of microbial induced precipitation calcite. Besides, the increase rate of microbial induced precipitation calcite gradually decreases because of the reduction of microbe absorption content with the decreasing pore size in bio-sandstone.
基金Funded by the National Natural Science Foundation of China(No.51702238)
文摘Bacillus subtilis was selected as the suitable microorganism,which could produce alkaline phosphatase and constantly hydrolyzed phosphate monoester in the mixture solution of bacteria with substrate,and then the PO4^3-was obtained.Bio-phosphate cement was prepared by alkaline earth element（Ba）ions reacting with PO4^3-in the mixture solution.Structure,size and thermal properties of the bio-phosphate cement were characterized by energy dispersive X-ray spectroscopy（EDS）,X-ray diffraction（XRD）,scanning electron microscopy（SEM）,and particle size analysis.The average crystallite sizes of chem-BaHPO4 and bio-BaHPO4corresponded to 11.99 and 24.13μm,respectively.Chem-BaHPO4 and bio-BaHPO4 were then adopted to bind loose sand particles.The results indicated that loose sand particles can be well cemented by the bio-BaHPO4powder into a bio-sandstone with a certain mechanical properties,and the average compressive strength of the bio-sandstones can be up to 0.83 MPa when the curing time was 14 d.Along with the method in future studies,there will be multiple new opportunities for engineering applications,for instance,the treatment of sandy soil foundation,remediation of heavy metals in contaminated soil,and so on.
