[目的]通过检测黄连素对三阴乳腺癌(triple-negative breast cancer,TNBC)细胞株MDA-MB-231细胞凋亡及葡萄糖调节蛋白78(glucose regulated protein 78,GRP78)表达的影响,探讨黄连素抗TNBC的机制。[方法]以MTT法检测不同浓度黄连素对MDA...[目的]通过检测黄连素对三阴乳腺癌(triple-negative breast cancer,TNBC)细胞株MDA-MB-231细胞凋亡及葡萄糖调节蛋白78(glucose regulated protein 78,GRP78)表达的影响,探讨黄连素抗TNBC的机制。[方法]以MTT法检测不同浓度黄连素对MDA-MB-231细胞增殖的影响,筛选黄连素的处理浓度。将MDA-MB-231细胞分为对照组、40μmol·L^(-1)黄连素组、60μmol·L^(-1)黄连素组、免疫球蛋白重链结合蛋白诱导剂X(immunoglobulin heavy chain binding protein inducer X,BiX)组,以及BiX联合40μmol·L^(-1)黄连素组、BiX联合60μmol·L^(-1)黄连素组。相应药物处理24h后,应用流式细胞术检测细胞凋亡情况,并以Western blot检测细胞GRP78和凋亡相关基因Bcl-2、Bax的表达。[结果]40μmol·L^(-1)以上浓度的黄连素可剂量依赖性抑制MDA-MB-231细胞增殖。与对照组比较,BiX组细胞凋亡率降低(P<0.05)、GRP78和Bcl-2表达增高(P<0.01,P<0.001)、Bax表达减低(P<0.05)。与BiX组比较,BiX联合40、60μmol·L^(-1)黄连素组细胞凋亡率增加(P<0.01),GRP78和Bcl-2表达减低(P<0.01,P<0.01),Bax表达增加(P<0.01)。[结论]黄连素可以促进MDA-MB-231细胞凋亡,其机制可能是通过下调GRP78的表达,从而降低Bcl-2表达,并促进Bax的表达。展开更多
A 59-year-old woman was admitted to our hospital because of recurrent follicular lymphoma(FL).Colonoscopic examination revealed a rectal submucosal tumor(SMT)without any erosions and ulcers.In this patient,it was diff...A 59-year-old woman was admitted to our hospital because of recurrent follicular lymphoma(FL).Colonoscopic examination revealed a rectal submucosal tumor(SMT)without any erosions and ulcers.In this patient,it was difficult to distinguish non-Hodgkin's lymphoma(NHL)invasion from other disorders of the colon including carcinoid tumor merely based on endoscopic findings.Histopathologic and immunohistochemical studies on biopsy specimens showed an infiltration of atypical lymphocytes that were positive for CD20 and BCL2 but negative for UCHL-1.Fluorescence in situ hybridization on paraffin-embedded tissue sections (T-FISH)identified a translocation of BCL2 with IGHgene. Based on these findings,the tumor was defined as an invasion of FL.T-FISH method is useful for the detection of a monoclonality of atypical lymphocytes in an SMT of the gastrointestinal tract,and particularly for the detection of chromosomal translocations specific to lymphoma subtypes.展开更多
文摘[目的]通过检测黄连素对三阴乳腺癌(triple-negative breast cancer,TNBC)细胞株MDA-MB-231细胞凋亡及葡萄糖调节蛋白78(glucose regulated protein 78,GRP78)表达的影响,探讨黄连素抗TNBC的机制。[方法]以MTT法检测不同浓度黄连素对MDA-MB-231细胞增殖的影响,筛选黄连素的处理浓度。将MDA-MB-231细胞分为对照组、40μmol·L^(-1)黄连素组、60μmol·L^(-1)黄连素组、免疫球蛋白重链结合蛋白诱导剂X(immunoglobulin heavy chain binding protein inducer X,BiX)组,以及BiX联合40μmol·L^(-1)黄连素组、BiX联合60μmol·L^(-1)黄连素组。相应药物处理24h后,应用流式细胞术检测细胞凋亡情况,并以Western blot检测细胞GRP78和凋亡相关基因Bcl-2、Bax的表达。[结果]40μmol·L^(-1)以上浓度的黄连素可剂量依赖性抑制MDA-MB-231细胞增殖。与对照组比较,BiX组细胞凋亡率降低(P<0.05)、GRP78和Bcl-2表达增高(P<0.01,P<0.001)、Bax表达减低(P<0.05)。与BiX组比较,BiX联合40、60μmol·L^(-1)黄连素组细胞凋亡率增加(P<0.01),GRP78和Bcl-2表达减低(P<0.01,P<0.01),Bax表达增加(P<0.01)。[结论]黄连素可以促进MDA-MB-231细胞凋亡,其机制可能是通过下调GRP78的表达,从而降低Bcl-2表达,并促进Bax的表达。
文摘A 59-year-old woman was admitted to our hospital because of recurrent follicular lymphoma(FL).Colonoscopic examination revealed a rectal submucosal tumor(SMT)without any erosions and ulcers.In this patient,it was difficult to distinguish non-Hodgkin's lymphoma(NHL)invasion from other disorders of the colon including carcinoid tumor merely based on endoscopic findings.Histopathologic and immunohistochemical studies on biopsy specimens showed an infiltration of atypical lymphocytes that were positive for CD20 and BCL2 but negative for UCHL-1.Fluorescence in situ hybridization on paraffin-embedded tissue sections (T-FISH)identified a translocation of BCL2 with IGHgene. Based on these findings,the tumor was defined as an invasion of FL.T-FISH method is useful for the detection of a monoclonality of atypical lymphocytes in an SMT of the gastrointestinal tract,and particularly for the detection of chromosomal translocations specific to lymphoma subtypes.
