To detect the genome of viruses (in environmental and clinical samples), we use electrophoresis running buffer after PCR reaction. Also, electrophoresis buffers were used widely to separate any DNA molecule. In this p...To detect the genome of viruses (in environmental and clinical samples), we use electrophoresis running buffer after PCR reaction. Also, electrophoresis buffers were used widely to separate any DNA molecule. In this paper, we used four types of previously known electrophoresis buffers to compare which is easy for preparation, simple in structure, low cost and good performance in agarose gel electrophoresis. For this, we used two agarose concentration (1%, 2%) and two types of DNA ladder (100 bp, 1 kb) represent both smaller and larger sizes of molecule for each type of buffers, from the result we found in first level both supper buffer and TAE buffer with good performance and in second level we found bicarbonate buffer also with good performance also. Finally, we found the tang buffer cannot pose any electrophoretic activity on DNA agarose gel electrophoresis.展开更多
Continuous intra-arterial blood gas monitoring is used clinically in hospital and intensive care unit settings.The blood gas can be detected by the colormetric indicators which are sealed in the silicone membrane.The ...Continuous intra-arterial blood gas monitoring is used clinically in hospital and intensive care unit settings.The blood gas can be detected by the colormetric indicators which are sealed in the silicone membrane.The glass tubing of the sensor should be as thin as possible since it affects response time and the bicarbonate buffer shifts the sensitivity of the sensor. In this paper,the sensitivity shifts by the bicarbonate buffer was researched.When carbon dioxide diffuses into the indicator, the carbon acid is produced and the bicarbonate buffer is used to control internal acidity.Because the sensitivity of the pCO_2 sensor is increased by controlling the concentration of the bicarbonate buffer,the bicarbonate buffer is used to obtain the optimum resolution in this investigation.Moreover,the different concentrations of the bicarbonate buffer for blood gas measurements are discussed in detail.展开更多
文摘To detect the genome of viruses (in environmental and clinical samples), we use electrophoresis running buffer after PCR reaction. Also, electrophoresis buffers were used widely to separate any DNA molecule. In this paper, we used four types of previously known electrophoresis buffers to compare which is easy for preparation, simple in structure, low cost and good performance in agarose gel electrophoresis. For this, we used two agarose concentration (1%, 2%) and two types of DNA ladder (100 bp, 1 kb) represent both smaller and larger sizes of molecule for each type of buffers, from the result we found in first level both supper buffer and TAE buffer with good performance and in second level we found bicarbonate buffer also with good performance also. Finally, we found the tang buffer cannot pose any electrophoretic activity on DNA agarose gel electrophoresis.
文摘Continuous intra-arterial blood gas monitoring is used clinically in hospital and intensive care unit settings.The blood gas can be detected by the colormetric indicators which are sealed in the silicone membrane.The glass tubing of the sensor should be as thin as possible since it affects response time and the bicarbonate buffer shifts the sensitivity of the sensor. In this paper,the sensitivity shifts by the bicarbonate buffer was researched.When carbon dioxide diffuses into the indicator, the carbon acid is produced and the bicarbonate buffer is used to control internal acidity.Because the sensitivity of the pCO_2 sensor is increased by controlling the concentration of the bicarbonate buffer,the bicarbonate buffer is used to obtain the optimum resolution in this investigation.Moreover,the different concentrations of the bicarbonate buffer for blood gas measurements are discussed in detail.
