Inhibitory activity of Bifidobacterium adolescent combined with cisplatin on melanoma in mice and its mechanism

The aim of this study is to explore inhibitory activity of Bifidobacterium adolescent combined with cis-platin on the growth of melanoma(B16)in mice and the underlying mechanism.C57 mice were inoculated with B16 cancer cells to construct mouse model of melanoma and treated with bifidobacterium adolescent combined with cisplatin.Ratios of inhibitory activity on the growth of melanoma(B16)were calculated.Pathology changes of the tumor were observed by HE staining.B16 cell cycles were examined on a flow cytometer.Lymphocyte prolif-eration was measured with MTT assay and the T-cell sub-set was measured by double marked fluorescence.When bifidobacterium of 1010 cfu/L was injected,the ratio of inhibitory activity on the growth of melanoma(B16)reached 54%,which was similar to that of cisplatin group.The ratio of inhibitory activity reached 74.45%when the mice were treated by bifidobacterium combined with cis-platin.HE staining shows that bifidobacterium inhibited B16 cell proliferation and enhanced the cisplatin`s killing activity on B16 cells.The results of flow cytometry demonstrated that B16 cell proliferation was arrested at G1 stage after treatment with bifidobacterium.The B16 cell proliferation was arrested at S stage after treatment with cisplatin.The CD4+percentage increased and the difference was significant compared with the normal group after treatment with bifidobacterium,indicating that T-cell immune activity was enhanced.Treatment with bifidobacterium combined with cisplatin can enhance the inhibitory activity on the growth of melanoma(B16)of cisplatin.The mechanism of the inhibitory activity on B16 cell proliferation is correlated with the enhanced immune activity in mice.

青春双歧杆菌凝固型酸奶研制及选择性培养基比较

以鲜牛奶、白砂糖为主要原料,以青春双歧杆菌、保加利亚乳杆菌、嗜热链球菌为发酵剂,制成营养保健的青春双歧杆菌凝固型酸奶。通过试验确定了青春双歧杆菌凝固型酸奶最佳工艺为:白砂糖8%,接种量3%,保加利亚乳杆菌嗜热链球菌青春双歧杆菌=114,发酵4h。结合MRS培养基和LM-MRS培养基可以对青春双歧杆菌凝固型酸奶中三种乳酸菌分别计数。青春双歧杆菌凝固型酸奶中保加利亚乳杆菌活菌数为8.0×108cfu·mL-1,嗜热链球菌活菌数为8.5×108cfu·mL-1,青春双歧杆菌活菌数为2.0×106cfu·mL-1。

青春双歧杆菌工业化培养基以及生长条件的筛选

以廉价的商业化产品作为原材料,以提高发酵液中的青春双歧杆菌活菌数为目的,通过单因素试验筛选并最终确定能够用于规模化生产青春双歧杆菌的廉价工业化培养基成分,即:脱脂乳8%(W/V)、双歧因子A1%(W/V)、双歧因子B1%(W/V)。结果表明,在此培养基基础上,筛选出了青春双歧杆菌的最佳生长条件:最佳接种量为1%(V/V)、最佳培养温度为37℃。在此优化的生长条件下,测定青春双歧杆菌的生长曲线和pH值变化,选取在厌氧条件下培养时间9h为最佳培养时间,此时青春双歧杆菌活菌数最高可达1.16×109cfu·mL-1。

青春双歧杆菌酸奶治疗妊娠期便秘的临床观察

目的观察和评价青春双歧杆菌酸奶治疗妊娠中、后期便秘的临床疗效。方法将152例妊娠期便秘患者随机分为治疗组和对照组,均给予基础治疗,治疗组同时每天晚饭后1h饮用200 mL最低活菌浓度在108 cfu/mL以上的青春双歧杆菌酸奶,10d后观察疗效。结果治疗组治疗总有效率为97.37%,显著高于对照组的51.32%(P<0.01),治疗组大便频率提高到每周4.60±2.01次,显著高于对照组的每周2.95±1.05次(P<0.01),治疗组无不良反应。结论青春双歧杆菌酸奶治疗妊娠中、后期便秘疗效显著。