In this study, we developed a tailored capillary sorbent for bilirubin removal. For immobilized bioligand, capillaries were grafted with epoxy groups using RIGP. The HSA immobilized capillaries has a high affinity ads...In this study, we developed a tailored capillary sorbent for bilirubin removal. For immobilized bioligand, capillaries were grafted with epoxy groups using RIGP. The HSA immobilized capillaries has a high affinity adsorption capacity （71.2 mg bilirubin/g polymer） and a shorter adsorption equilibrium time （about 60 min）.展开更多
Bilirubin removal from human plasma was obtained via an affinity microporous polytetrafluoroethylene（MPTFE） capillary. The new adsorbent comprised grafted glycidyl methacrylate（GMA） via radiation-induced polymeriz...Bilirubin removal from human plasma was obtained via an affinity microporous polytetrafluoroethylene（MPTFE） capillary. The new adsorbent comprised grafted glycidyl methacrylate（GMA） via radiation-induced polymerization as hydrophilic coating and reactive sites; ethylenediamine（EDA） as a spacer arm; Cibacron Blue F3GA（CB F3GA） as an affinity ligand; MPTFE capillary as the supporting matrix. The average density of CB F3GA attachment to MPTFE capillaries was found to be 136.5 μmol/g. The capacity of bilirubin adsorbed on affinity MPTFE capillaries is 76.1 mg bilirubin/g polymer（at 25℃）. This new adsorbent has advantages over both membrane and traditional micro-column, and this system is stable and easy to operate. The results of blood tests suggest the CB F3GA affinity capillary has good blood compatibility.展开更多
文摘In this study, we developed a tailored capillary sorbent for bilirubin removal. For immobilized bioligand, capillaries were grafted with epoxy groups using RIGP. The HSA immobilized capillaries has a high affinity adsorption capacity （71.2 mg bilirubin/g polymer） and a shorter adsorption equilibrium time （about 60 min）.
基金Supported by the National Natural Science Foundation of China(No.29405038)
文摘Bilirubin removal from human plasma was obtained via an affinity microporous polytetrafluoroethylene（MPTFE） capillary. The new adsorbent comprised grafted glycidyl methacrylate（GMA） via radiation-induced polymerization as hydrophilic coating and reactive sites; ethylenediamine（EDA） as a spacer arm; Cibacron Blue F3GA（CB F3GA） as an affinity ligand; MPTFE capillary as the supporting matrix. The average density of CB F3GA attachment to MPTFE capillaries was found to be 136.5 μmol/g. The capacity of bilirubin adsorbed on affinity MPTFE capillaries is 76.1 mg bilirubin/g polymer（at 25℃）. This new adsorbent has advantages over both membrane and traditional micro-column, and this system is stable and easy to operate. The results of blood tests suggest the CB F3GA affinity capillary has good blood compatibility.
