Screened atrazine-mineralizing bacterium―Pseudomonas W4 was embedded inside an improved PVA- H3BO3 embedment matrix to make bio-beads to degrade atrazine. The atrazine degradation characteristics were studied. The pr...Screened atrazine-mineralizing bacterium―Pseudomonas W4 was embedded inside an improved PVA- H3BO3 embedment matrix to make bio-beads to degrade atrazine. The atrazine degradation characteristics were studied. The preparation procedure of bio-beads was as follows： （1） preparing a mixture of 100, 12.5, 10, 1.5 and 1 g/L PVA, bentonite（Ca）, activated carbon powder, sodium alginate and centrifuged Pseudomonas W4 bacterium, respectively; （2） the mixture was dropped into a gently stirred cross linker solution（pH=6.7） and cured at 10 °C for 24 h. The optimal atrazine degradation conditions by bio-beads were as follows： pH=7, the auxiliary carbon source was glucose, and the concentration of glucose was greater than 325 mg/L. The bio-beads demonstrated stronger tolerance ability than the free microorganism to the increase of PCBs, hydrogen ion and hydroxide ion. SEM images show the uniform distribution of the microorganism inside bio-beads and the porous cross-linked structure of bio-beads which provides excellent mass transfer capacity.展开更多
文章以粒径为5 mm的聚乙烯醇(PVA)凝胶颗粒为生物载体,研究其对厌氧污泥颗粒化进程及反应器运行效果的影响。在PVA颗粒污泥初成体形成的情况下,UASB反应器连续运行74 d。试验结果表明:经过32 d的培养,得到沉降性能优良的PVA颗粒污泥,反...文章以粒径为5 mm的聚乙烯醇(PVA)凝胶颗粒为生物载体,研究其对厌氧污泥颗粒化进程及反应器运行效果的影响。在PVA颗粒污泥初成体形成的情况下,UASB反应器连续运行74 d。试验结果表明:经过32 d的培养,得到沉降性能优良的PVA颗粒污泥,反应器COD去除率高达97%,容积负荷由1.05 kg COD·m-3d-1提升至13.33 kg COD·m-3d-1,水力停留时间由35 h缩短至8 h;运行结束时,COD去除率稳定在90%左右,容积负荷达到13.54 kg COD·m-3d-1。通过扫描电镜观察到成熟PVA颗粒污泥外表面丝状菌上交联生长着杆菌、球菌,内部主要附着丝状菌。展开更多
基金Supported by the National Natural Science Foundation of China(Nos.40702039, 20090879029)
文摘Screened atrazine-mineralizing bacterium―Pseudomonas W4 was embedded inside an improved PVA- H3BO3 embedment matrix to make bio-beads to degrade atrazine. The atrazine degradation characteristics were studied. The preparation procedure of bio-beads was as follows： （1） preparing a mixture of 100, 12.5, 10, 1.5 and 1 g/L PVA, bentonite（Ca）, activated carbon powder, sodium alginate and centrifuged Pseudomonas W4 bacterium, respectively; （2） the mixture was dropped into a gently stirred cross linker solution（pH=6.7） and cured at 10 °C for 24 h. The optimal atrazine degradation conditions by bio-beads were as follows： pH=7, the auxiliary carbon source was glucose, and the concentration of glucose was greater than 325 mg/L. The bio-beads demonstrated stronger tolerance ability than the free microorganism to the increase of PCBs, hydrogen ion and hydroxide ion. SEM images show the uniform distribution of the microorganism inside bio-beads and the porous cross-linked structure of bio-beads which provides excellent mass transfer capacity.
文摘文章以粒径为5 mm的聚乙烯醇(PVA)凝胶颗粒为生物载体,研究其对厌氧污泥颗粒化进程及反应器运行效果的影响。在PVA颗粒污泥初成体形成的情况下,UASB反应器连续运行74 d。试验结果表明:经过32 d的培养,得到沉降性能优良的PVA颗粒污泥,反应器COD去除率高达97%,容积负荷由1.05 kg COD·m-3d-1提升至13.33 kg COD·m-3d-1,水力停留时间由35 h缩短至8 h;运行结束时,COD去除率稳定在90%左右,容积负荷达到13.54 kg COD·m-3d-1。通过扫描电镜观察到成熟PVA颗粒污泥外表面丝状菌上交联生长着杆菌、球菌,内部主要附着丝状菌。
