Objective: The present study consisted of challenging the extract of kombucha and the fungus Ganoderma reported as hypoglycemic and used as alternative treatments against diabetes on the number and morphology of islet...Objective: The present study consisted of challenging the extract of kombucha and the fungus Ganoderma reported as hypoglycemic and used as alternative treatments against diabetes on the number and morphology of islets of Langerhans. Material and Methods: 64 Wistar rats were used in 4 groups: one control, three experimental, streptozotocin, Kombucha y Ganoderma induced diabetes with streptozotocin. Divided into four post-induction stages at 2, 15, 30 and 45 days of treatment, sacrificing 4 rats at each stage, to perform the morphological analysis of the pancreas. Results: A decrease in the islets of Langerhans in size, volume and the number of cells within them was identified for the streptozotocin group from the second stage until almost disappearing due to diabetes, in the groups of Kombucha y Ganoderma the same was observed but they were recovered with the extract treatments and the average number of islets was similar in these groups, the group of Ganoderma. Conclusion: Under the conditions of this work, a protective and regenerative effect of both extracts is identified.展开更多
Objective To evaluate the effect of oral administration of Kombucha tea (K-tea) on lead induced oxidative stress. Methods Sprague Dawley rats were administered 1 mL of 3.8% lead acetate solution daily alone or in comb...Objective To evaluate the effect of oral administration of Kombucha tea (K-tea) on lead induced oxidative stress. Methods Sprague Dawley rats were administered 1 mL of 3.8% lead acetate solution daily alone or in combination with K-tea orally for 45 d, and the antioxidant status and lipid peroxidation were evaluated. Results Oral administration of lead acetate to rats enhanced lipid peroxidation and release of creatine phosphokinase and decreased levels of reduced glutathione (GSH) and antioxidant enzymes (superoxide dismutase, SOD and glutathione peroxidase, GPx). Lead treatment did not alter humoral immunity, but inhibited DTH response when compared to the control. Lead administration also increased DNA fragmentation in liver. Oral administration of Kombucha tea to rats exposed to lead decreased lipid peroxidation and DNA damage with a concomitant increase in the reduced glutathione level and GPx activity. Kombucha tea supplementation relieved the lead induced immunosuppression to appreciable levels. Conclusion The results suggest that K-tea has potent antioxidant and immunomodulating properties.展开更多
[Objective] To optimize solid-state fermentation of South China 5 (SC5) cassava-leucaena protein feed by kombucha. [Method] The SC5 cassava-leucaena protein feed was fermented by kombucha. The fermentation condition...[Objective] To optimize solid-state fermentation of South China 5 (SC5) cassava-leucaena protein feed by kombucha. [Method] The SC5 cassava-leucaena protein feed was fermented by kombucha. The fermentation conditions were optimized. [ Result] The optimum conditions of solid-state fermentation were as follows: cassava and leucaena weight ratio, 7:3; fermentation time, 6 d; kombucha volume, 10 ml; fermentation temperature, 30 ℃; and water volume, 200 ml. The content of crude protein in feed was substantially increased and reached 23.64%. [ Conclusion] The optimized fermentation process greatly improves utilization of the SC5 cassava-leucaena protein feed.展开更多
[Objective] The aim was to study if the fermentation broth of Kombucha has protein with antimicrobial activity.[Method] The effects of different cultivation time on cell concentration,pH,total protein concentration an...[Objective] The aim was to study if the fermentation broth of Kombucha has protein with antimicrobial activity.[Method] The effects of different cultivation time on cell concentration,pH,total protein concentration and inhibition zone were studied.The fermentation broth of Kombucha on the sixth day was adjusted to different pH.Fermentation broth treated with protease was put into the plates of Escherichia coli,Bacillus cereue and Staphylococcus aureus as control,as well as ampenicilin.The zone of broth inhibition was measured.[Result] The concentration of general protein was of positive relevance to the antimicrobial activity of Kombucha fermentation broth.The zone of inhibition decreased with rising pH and was the lowest when pH was 7.Later,as pH increasing,it enlarged.The inhibition effect of processed protease reduced remarkably.[Conclusion] The fermentation broth of Kombucha revealed antimicrobial proteins.展开更多
Objective: to evaluate the protective effect of kombucha and its components not polar, in pancreas of diabetic rats treated with streptozotocin. Material and methods: ninety-six male Wistar rats of 120 to 170 g of PC ...Objective: to evaluate the protective effect of kombucha and its components not polar, in pancreas of diabetic rats treated with streptozotocin. Material and methods: ninety-six male Wistar rats of 120 to 170 g of PC were used;four groups of 24 animals were formed: control (GC), control treatment (GCTx), kombucha treatment (GTxK) and treatment with non-polar components of kombucha (GTxCNP). Diabetes was induced in the last three groups with an intraperitoneal injection of streptozotocin at a dose of 65 mg/kg BW. At the same time, treatment was started in the different groups;GC and GCTx groups were administered, orally, 1 ml of sterile saline solution;to the GTxK group 324 mg/kg of kombucha;and to GTxCNP 0.006 mg/kg of the non-polar components. For the histopathological study, pancreas samples were obtained on days 1, 7, 14 and 21 and fixed in formalin. They were processed with the histological technique, 5-micron-thick sections were made and stained with the hematoxylin-eosin technique. Finally, the number of islets of Langerhans per field observed with the 10x objective was quantified. The results were subjected to a one-way analysis of variance and Tukey’s test (p Results: Significant changes in the number of islets were observed in histological sections of the pancreas. The groups treated with streptozotocin showed a significant decrease in the number of islets 7 days after starting treatment;Likewise, their number was lower in the GCTx group compared to the GTxK and GTxCNP groups (p ≤ 0.05). At 14 and 21 days, the lowest number of islets was maintained, although only in the GCTx and GTxK groups (p ≤ 0.05). Streptozotocin is a drug that can be used in rats to induce an experimental model of diabetes with an effect that can last up to 21 days. Conclusions: This fermented beverage and its non-polar components can be a complementary treatment alternative for diabetes mellitus, since it prevents further damage to the β cells of the pancreas and the Islets of Langerhans. Specific additional studies on the main, non-polar components of kombucha are suggested to know the mechanism of protection on pancreatic islets.展开更多
This study examined the survival of acid-adapted E. coli O157 in kombucha during fermentation and refrigerated kombucha mixed with fruit juices. Acidic and non-acidic kombucha mixes were fermented at 25°C usin...This study examined the survival of acid-adapted E. coli O157 in kombucha during fermentation and refrigerated kombucha mixed with fruit juices. Acidic and non-acidic kombucha mixes were fermented at 25°C using a commercially available starter culture and inoculated with a 5-strain mixture of acid-adapted E. coli O157. There was >5-log reduction in the pathogen count for both starter mixes within 7 days of fermentation. For the kombucha-juice blends at refrigerated temperature, 14 ml of lemon, apple, orange, and mango juices were mixed with 186 ml kombucha separately. The treatments were inoculated with a 5-strain mixture of acid-adapted E. coli O157 and incubated at 5°C for 14 days. >5-log reduction in the pathogen count was observed in lemon, control, and mango juice blend after 1, 3, and 14 days, respectively. The total reduction in pathogen count in the apple and orange juice blend after 14 days was 4.43 and 4.12 log CFU/ml, respectively. The inability of the kombucha fruit blend to cause a 5-log reduction of E. coli O157 suggests the need for following strict hygienic and good sanitation practices during blending and bottling for home fermenters and an approved HACCP plan for foodservice operators to ensure product safety.展开更多
In order to assess the impact of the early and late tissue repair of Kombucha extract on incised wounds in animal models, 24 Wistar male rats were used and divided into three groups: one of the groups received a topic...In order to assess the impact of the early and late tissue repair of Kombucha extract on incised wounds in animal models, 24 Wistar male rats were used and divided into three groups: one of the groups received a topical cure on the wound with Brosin, and the other received the Kombucha extract. The third group did not receive any treatment, working as a control group. The objective was to evaluate and compare the Kombucha extract with a commercial healing product. Each one of the animals took a 1 cm wound in depth and length at a femur level on a side of the left thigh. The healing process was evaluated on an early and late phase by performing a morphometric and morphological analysis under the assumption of a faster recovery with the use of Kombucha. The results showed that there was a poor recovery in the control group. On the other hand, the other two groups, Brosin and Kombucha, were similar, with little inflammation and a high cell proliferation and migration along with basal array pulls of elastin and collagen which served for angiogenesis, repair and renovation of the new tissue.展开更多
Introduction: Diabetes is a disease characterized by an increase in blood glucose due to disorders in insulin production, this disease is widely distributed throughout the world. Objective: to review and systematize t...Introduction: Diabetes is a disease characterized by an increase in blood glucose due to disorders in insulin production, this disease is widely distributed throughout the world. Objective: to review and systematize the general information on diabetes, as well as the traditional treatments and the findings on kombucha as an alternative method. Method: the information retrieval technique or literature review was used on the topics of diabetes, allopathic methods, kombucha, preparation procedures, chemical and microbiological components and pharmacological effects of the drink. Results: Various sources of information were found that explain the definition of diabetes and its classification. it commonly presents in four ways depending on the signs, the common ones being type 1, associated with destruction of the β cells of the pancreas and mainly affects minors, and type 2, which is related to genetic and environmental problems and It affects people over 18 years of age, and less frequently gestational diabetes and MODY type. Traditional drugs such as sulfonylureas, glinides and biguanides, thiazolidinediones and α-glucosidase inhibitors are used to control this disease, or alternative therapies are used such as kombucha, a fermented beverage that has its origins in China and is spread worldwide by its beneficial effects in chronic degenerative diseases such as diabetes, due to its chemical and microbiological content. Conclusion: The information establishes important data on kombucha as one of the complementary alternatives for the treatment of diabetes.展开更多
With the objective to evaluate the properties, curatives of kombucha extract as an alternative treatment were used;20 sheep creoles weaned with stunting, respiratory diseases, parasitic and malnutrition were divided i...With the objective to evaluate the properties, curatives of kombucha extract as an alternative treatment were used;20 sheep creoles weaned with stunting, respiratory diseases, parasitic and malnutrition were divided into control and experiment n = 10 groups;the first one was administered by ivermectin 1% (2 mg/kg) subcutaneous, with a reinforcement of closantel 5% (0.5 mg/kg) by oral route and enrofloxacin at 5% (3 mg/kg) intramuscularly for three consecutive days and the second group by oral route 20 ml of extract kombucha per animal each three days for about 4 months, was weighed at the beginning of each month;clinical tests were conducted and statistical data indicated no significant differences in weight gain 30.370 kg/p control, 31.290, 3.503 kg/p experimentally, some showed small differences in blood samples and in stool tests for example, control ended with Eimeria 1.00 ± 0.00, creatinine, 0.17 ± 0.04 mg/dl, uric acid 23.20 5.37 ± mg/dl, 46.12 ± 4.70 MCV, 7.21 ± 0.25 g/dl total protein, 38.20 10.06 ± 1.40 and segmented neutrophils ±1.07, and on the other hand the experimental in erythrocytes present 9783000.00 ± 1036072.17 million, in hemoglobin 13.43 ± 0.51 g/dl, hematocrit 38.87 ± 2.13, and platelet 422320.000 ± 52442.578 thousands. Both groups did not exceed normal parameters, however, physically the experimental animals were better and had no parasites or respiratory problems as opposed to controls.展开更多
文摘Objective: The present study consisted of challenging the extract of kombucha and the fungus Ganoderma reported as hypoglycemic and used as alternative treatments against diabetes on the number and morphology of islets of Langerhans. Material and Methods: 64 Wistar rats were used in 4 groups: one control, three experimental, streptozotocin, Kombucha y Ganoderma induced diabetes with streptozotocin. Divided into four post-induction stages at 2, 15, 30 and 45 days of treatment, sacrificing 4 rats at each stage, to perform the morphological analysis of the pancreas. Results: A decrease in the islets of Langerhans in size, volume and the number of cells within them was identified for the streptozotocin group from the second stage until almost disappearing due to diabetes, in the groups of Kombucha y Ganoderma the same was observed but they were recovered with the extract treatments and the average number of islets was similar in these groups, the group of Ganoderma. Conclusion: Under the conditions of this work, a protective and regenerative effect of both extracts is identified.
文摘Objective To evaluate the effect of oral administration of Kombucha tea (K-tea) on lead induced oxidative stress. Methods Sprague Dawley rats were administered 1 mL of 3.8% lead acetate solution daily alone or in combination with K-tea orally for 45 d, and the antioxidant status and lipid peroxidation were evaluated. Results Oral administration of lead acetate to rats enhanced lipid peroxidation and release of creatine phosphokinase and decreased levels of reduced glutathione (GSH) and antioxidant enzymes (superoxide dismutase, SOD and glutathione peroxidase, GPx). Lead treatment did not alter humoral immunity, but inhibited DTH response when compared to the control. Lead administration also increased DNA fragmentation in liver. Oral administration of Kombucha tea to rats exposed to lead decreased lipid peroxidation and DNA damage with a concomitant increase in the reduced glutathione level and GPx activity. Kombucha tea supplementation relieved the lead induced immunosuppression to appreciable levels. Conclusion The results suggest that K-tea has potent antioxidant and immunomodulating properties.
基金supported by Graduate Research Fund of Tropical Crops Genetic Resources Institute,Chinese Academyof Tropical Agricultural SciencesBreeding of New Varieties of High-Starch,Cold-Resistant and Drought-Resistant Cassava(nyhyzx07-013) Earmarked Fund for Modern Agro-Industry Technology Research System (nycytx-17)
文摘[Objective] To optimize solid-state fermentation of South China 5 (SC5) cassava-leucaena protein feed by kombucha. [Method] The SC5 cassava-leucaena protein feed was fermented by kombucha. The fermentation conditions were optimized. [ Result] The optimum conditions of solid-state fermentation were as follows: cassava and leucaena weight ratio, 7:3; fermentation time, 6 d; kombucha volume, 10 ml; fermentation temperature, 30 ℃; and water volume, 200 ml. The content of crude protein in feed was substantially increased and reached 23.64%. [ Conclusion] The optimized fermentation process greatly improves utilization of the SC5 cassava-leucaena protein feed.
基金Supported by Scientific Research Fund of Beijing Educational DepartmentBeijing Higher School Personnel Teaching Program
文摘[Objective] The aim was to study if the fermentation broth of Kombucha has protein with antimicrobial activity.[Method] The effects of different cultivation time on cell concentration,pH,total protein concentration and inhibition zone were studied.The fermentation broth of Kombucha on the sixth day was adjusted to different pH.Fermentation broth treated with protease was put into the plates of Escherichia coli,Bacillus cereue and Staphylococcus aureus as control,as well as ampenicilin.The zone of broth inhibition was measured.[Result] The concentration of general protein was of positive relevance to the antimicrobial activity of Kombucha fermentation broth.The zone of inhibition decreased with rising pH and was the lowest when pH was 7.Later,as pH increasing,it enlarged.The inhibition effect of processed protease reduced remarkably.[Conclusion] The fermentation broth of Kombucha revealed antimicrobial proteins.
文摘Objective: to evaluate the protective effect of kombucha and its components not polar, in pancreas of diabetic rats treated with streptozotocin. Material and methods: ninety-six male Wistar rats of 120 to 170 g of PC were used;four groups of 24 animals were formed: control (GC), control treatment (GCTx), kombucha treatment (GTxK) and treatment with non-polar components of kombucha (GTxCNP). Diabetes was induced in the last three groups with an intraperitoneal injection of streptozotocin at a dose of 65 mg/kg BW. At the same time, treatment was started in the different groups;GC and GCTx groups were administered, orally, 1 ml of sterile saline solution;to the GTxK group 324 mg/kg of kombucha;and to GTxCNP 0.006 mg/kg of the non-polar components. For the histopathological study, pancreas samples were obtained on days 1, 7, 14 and 21 and fixed in formalin. They were processed with the histological technique, 5-micron-thick sections were made and stained with the hematoxylin-eosin technique. Finally, the number of islets of Langerhans per field observed with the 10x objective was quantified. The results were subjected to a one-way analysis of variance and Tukey’s test (p Results: Significant changes in the number of islets were observed in histological sections of the pancreas. The groups treated with streptozotocin showed a significant decrease in the number of islets 7 days after starting treatment;Likewise, their number was lower in the GCTx group compared to the GTxK and GTxCNP groups (p ≤ 0.05). At 14 and 21 days, the lowest number of islets was maintained, although only in the GCTx and GTxK groups (p ≤ 0.05). Streptozotocin is a drug that can be used in rats to induce an experimental model of diabetes with an effect that can last up to 21 days. Conclusions: This fermented beverage and its non-polar components can be a complementary treatment alternative for diabetes mellitus, since it prevents further damage to the β cells of the pancreas and the Islets of Langerhans. Specific additional studies on the main, non-polar components of kombucha are suggested to know the mechanism of protection on pancreatic islets.
文摘This study examined the survival of acid-adapted E. coli O157 in kombucha during fermentation and refrigerated kombucha mixed with fruit juices. Acidic and non-acidic kombucha mixes were fermented at 25°C using a commercially available starter culture and inoculated with a 5-strain mixture of acid-adapted E. coli O157. There was >5-log reduction in the pathogen count for both starter mixes within 7 days of fermentation. For the kombucha-juice blends at refrigerated temperature, 14 ml of lemon, apple, orange, and mango juices were mixed with 186 ml kombucha separately. The treatments were inoculated with a 5-strain mixture of acid-adapted E. coli O157 and incubated at 5°C for 14 days. >5-log reduction in the pathogen count was observed in lemon, control, and mango juice blend after 1, 3, and 14 days, respectively. The total reduction in pathogen count in the apple and orange juice blend after 14 days was 4.43 and 4.12 log CFU/ml, respectively. The inability of the kombucha fruit blend to cause a 5-log reduction of E. coli O157 suggests the need for following strict hygienic and good sanitation practices during blending and bottling for home fermenters and an approved HACCP plan for foodservice operators to ensure product safety.
文摘In order to assess the impact of the early and late tissue repair of Kombucha extract on incised wounds in animal models, 24 Wistar male rats were used and divided into three groups: one of the groups received a topical cure on the wound with Brosin, and the other received the Kombucha extract. The third group did not receive any treatment, working as a control group. The objective was to evaluate and compare the Kombucha extract with a commercial healing product. Each one of the animals took a 1 cm wound in depth and length at a femur level on a side of the left thigh. The healing process was evaluated on an early and late phase by performing a morphometric and morphological analysis under the assumption of a faster recovery with the use of Kombucha. The results showed that there was a poor recovery in the control group. On the other hand, the other two groups, Brosin and Kombucha, were similar, with little inflammation and a high cell proliferation and migration along with basal array pulls of elastin and collagen which served for angiogenesis, repair and renovation of the new tissue.
文摘Introduction: Diabetes is a disease characterized by an increase in blood glucose due to disorders in insulin production, this disease is widely distributed throughout the world. Objective: to review and systematize the general information on diabetes, as well as the traditional treatments and the findings on kombucha as an alternative method. Method: the information retrieval technique or literature review was used on the topics of diabetes, allopathic methods, kombucha, preparation procedures, chemical and microbiological components and pharmacological effects of the drink. Results: Various sources of information were found that explain the definition of diabetes and its classification. it commonly presents in four ways depending on the signs, the common ones being type 1, associated with destruction of the β cells of the pancreas and mainly affects minors, and type 2, which is related to genetic and environmental problems and It affects people over 18 years of age, and less frequently gestational diabetes and MODY type. Traditional drugs such as sulfonylureas, glinides and biguanides, thiazolidinediones and α-glucosidase inhibitors are used to control this disease, or alternative therapies are used such as kombucha, a fermented beverage that has its origins in China and is spread worldwide by its beneficial effects in chronic degenerative diseases such as diabetes, due to its chemical and microbiological content. Conclusion: The information establishes important data on kombucha as one of the complementary alternatives for the treatment of diabetes.
文摘With the objective to evaluate the properties, curatives of kombucha extract as an alternative treatment were used;20 sheep creoles weaned with stunting, respiratory diseases, parasitic and malnutrition were divided into control and experiment n = 10 groups;the first one was administered by ivermectin 1% (2 mg/kg) subcutaneous, with a reinforcement of closantel 5% (0.5 mg/kg) by oral route and enrofloxacin at 5% (3 mg/kg) intramuscularly for three consecutive days and the second group by oral route 20 ml of extract kombucha per animal each three days for about 4 months, was weighed at the beginning of each month;clinical tests were conducted and statistical data indicated no significant differences in weight gain 30.370 kg/p control, 31.290, 3.503 kg/p experimentally, some showed small differences in blood samples and in stool tests for example, control ended with Eimeria 1.00 ± 0.00, creatinine, 0.17 ± 0.04 mg/dl, uric acid 23.20 5.37 ± mg/dl, 46.12 ± 4.70 MCV, 7.21 ± 0.25 g/dl total protein, 38.20 10.06 ± 1.40 and segmented neutrophils ±1.07, and on the other hand the experimental in erythrocytes present 9783000.00 ± 1036072.17 million, in hemoglobin 13.43 ± 0.51 g/dl, hematocrit 38.87 ± 2.13, and platelet 422320.000 ± 52442.578 thousands. Both groups did not exceed normal parameters, however, physically the experimental animals were better and had no parasites or respiratory problems as opposed to controls.