Effects of Biological Organic Fertilizer on Flue-cured Tobacco Chemical Composition

Through field experiment,special biological fertilizer and cake fertilizer were applied collectively to tobacco,and chemical compositions of Wanxi flue-cured tobacco leaves were researched.The results showed that the increase of biological organic fertilizer would significantly improve soil nutrient supplying capacity.Compared with conventional fertilization,the contents of reducing sugar,total sugar and starch in different parts of tobaccos which were applied with biological organic fertilizer were closer to standards of excellent tobacco.In the treatment,the ratio of reducing sugar and nicotine in tobacco was more suitable in middle and lower parts of the plant and the contents of total N,nicotine and chlorine in tobacco were lowered,improving tobacco quality.

Effects of Biological Bacterial Fertilizer on Carbon Metabolism Characteristics of Rhizosphere Soil Bacteria in Rice

The effects of biological bacterial fertilizer and chemical fertilizer on carbon metabolism characteristics of rhizosphere soil bacteria in rice were studied through a plot experiment.The results showed that the number and Mcintosh index of bacteria in rice rhizosphere soil increased significantly with the application of biological bacterial fertilizer.It was found that the AWCD(average well color development)value of the bacteria remarkably increased and the decomposition of carboxylic acids,amines and heterozygotes were accelerated when adding biological bacterial fertilizer at a proper weight percent.All in all,proper addition of biological bacterial fertilizer could increase the number and carbon metabolism of bacteria.The most appropriate application rate was 70%chemical fertilizer nitrogen+30%biological bacterial fertilizer nitrogen for rice production in Northern Jiangsu Province.

Chemical and Microbiological Parameters of Paddy Soil Quality as Affected by Different Nutrient and Water Regimes

A field experiment was conducted from 1999 to 2002 to compare and evaluate the effects of nutrient and water regimes on paddy soil quality by investigating soil chemical and microbiological parameters. Four nutrient regimes, a control, chemical fertilizers only (CF), chemical fertilizers with swine manure (SM), and chemical fertilizers with wheat straw (WS), and two soil moisture regimes, continuous waterlogging (CWL) and alternate wetting and drying (AWD), were investigated. With SM and WS total organic carbon and total nitrogen in the paddy soil were significantly higher (P < 0.05) than those with CF. A similar effect for organic amendments was observed in the soil light fraction organic C (LFOC), water-soluble carbohydrates (WSC), and water-soluble organic C (WSOC). CWL, in particular when swine manure was incorporated into the paddy soil, markedly decreased soil redox potential (Eh) and increased total active reducing substances (ARS). Meanwhile, as compared to CF, SM and WS significantly (P < 0.05) increased soil microbial biomass C (MBC) and mineralizable carbon, with differences in AWD being higher than CWL. In addition, SM and WS treatments significantly (P < 0.05) improved rice above-ground biomass and grain yield, with AWD being greater than CWL. Thus, for ecologically sustainable agricultural management of paddy soils, long-term waterlogging should be avoided when organic manure was incorporated into paddy soil.

Effects of Organic Fertilizer on Fruit Quality and Acidified Soil Chemical Properties in Yantai Orchard

[Objective] This study aimed to investigate the improving effect of organic fertilizer on acidified soil as well as their effects on fruit quality and quantity in Yantai orchard.[Method] Plot experiment was conducted to investigate the effects of organic fertilizer on fruit yield and quality of Red Fuji and chemical properties of acidified soil.[Result] The apple yield in acidified soil applied with organic fertilizer all increased.Under the application of biological organic fertilizer,the apple yield was higher,and it was 8.92% higher than that in the control group.Under the mixed application of chemical fertilizer and biological organic fertilizer,the growth and development of apple trees were improved,and the total soluble solid(TSS) content,vitamin C(Vc) content and TSS-acid ratio in mature apples all increased.The application of organic fertilizer significantly reduced soil acidity.Compared with those in the control group,the soil p H value,organic matter content and alkali-hydrolyzable nitrogen content under the application of biological organic fertilizer were increased by 8.33%,15.10% and 30.80%,respectively.[Conclusion] The application of biological organic fertilizer could improve the yield of apple in acidified soil.

Effects of Sinochem Microbial Organic Fertilizer on Tea Plants

[Objectives]This study was conducted to explore the application effects of Sinochem microbial organic fertilizer on tea plants.[Methods]Through the experiment and demonstration of Sinochem microbial organic fertilizer,its effects on the yield and quality of tea and the physical and chemical quality of soil were explored.[Results]Compared with the conventional organic fertilizer cake fertilizer,the fertilizer could increase the weight of freshly picked bud leaves,increase the yield of tea,and thus promote the growth of tea plants.It increased the contents of chlorophyll components,amino acids and tea polyphenols,the main functional substances of tea,which was conducive to improving the internal quality of tea.It improved the physical and chemical properties of soil,and increased the contents of organic matter,alkali-hydrolyzable nitrogen and available phosphorus in tea garden soil.It also had a good effect in improving soil environmental health indexes and physical and chemical properties,and did not contain heavy metals and plant growth hormones.[Conclusions]This study provides a theoretical basis for the popularization and application of Sinochem microbial organic fertilizer.

Physical-biological Collaborative Prevention and Control of Facility Strawberry Continuous Cropping Diseases

The aim was to develop green strawberry varieties,control soil-borne diseases from facility strawberry continuous cropping,improve the safety and quality of strawberries and promote the healthy and stable development of the strawberry industry. Based on the production practices in recent years,we summarized the physical-biological collaborative prevention and control technology for the disease of facility strawberry continuous cropping facilities,established the technical goals,the main types of soil-borne diseases,and the physical-biological collaborative control prevention and control technologies,mainly including the specific methods and technical points of solar high temperature disinfection technology and biological bacterial fertilizer（ agent） treatment technology.

Bioremediation of Soil Pollution in Orchard

[ Objective ] The aim was to study the bioremediation mechanism of soil pollution. [ Method ] The effects of applying biological organic fertilizers on the bioremediation of soil pollution in orchard were studid by experiment in orchard field and soil simulative experiment. [ Result] The biological organic fertilizers improved the activities of enzymes like polyphenol oxidase, urease, phosphatase, etc. in root-zone soil, promoted the passivation of heavy metals like Cd^2＋ , Pb^2＋ , Cr^3＋ , As^8＋ , etc. in root-zone soil, increased the quantities of useful active bacterium like beneficial fungi, actinomycetes, bacterium, etc. and decreased the quantities of harmful biology （like Fusarium oxysporum, Moniliophthora roreri, Ruselliniu necutrix/Helicobasidium mompa, nematode, etc. [ Conclusion] The study results provide some references for the popularization and application of biological organic fertilizers on fruit trees.

Rice (Oryza sativa L.) nutrient management using mycorrhizal fungi and endophytic Herbaspirillum seropedicae

Integrated nutrient management with biological and chemical fertilizers can improve rice （Oryza safiva L.） productivity, bio-fortification, soil health and fertility. Accordingly, this study was planned to evaluate the combined effects of biological fertilizers including arbuscular mycorrhizal （AM） fungi （Glomus mosseae） and free-living nitrogen-fixing bacteria （Herbaspi- rillum seropedicae）, as well as chemical fertilizers on the yield and nutrient contents of wetland rice under field conditions. Seedlings were inoculated with AM fungi and the bacteria in the nursery and were then transplanted to the field. The experi- ment was carried out as a split factorial design with three replicates. Treatments included three rates of nitrogen （N 1, N2 and N3） and phosphorous （P1, P2 and P3） fertilizers （100, 75 and 50% of the optimum level） in the main plots and mycorrhizal and bacterial treatments in the sub plots. The total of urea （g） used per plot was equal to N1=200, N2=150 and N3=100 at three different growth stages （seeding, tillering and heading） and the total of P （g） per plot used once at seeding using triple super phosphate including P1 =16, P2=13 and P3=10. Plant growth and yield as well as the concentration of nitrogen （N）, phosphorous （P）, potassium （K）, iron （Fe）, and zinc （Zn） were measured in the soil, straw and grains. N-fertilizer and biological fertilizers had significant effects on root, shoot and grain yield of rice, however, P-fertilizer just significantly affected root and shoot dry weights. Interestingly, analyses of variance indicated that biological fertilization significantly affected all the experimental treatments except straw N. AM fungi, N1 and P1 resulted in the highest rate of rice growth and yield. The interactions of chemical and biological fertilization resulted in significant effects on grain Zn, Fe, P, and N as well as soil Fe, K and N. The highest rate of grain nutrient uptake was resulted by the combined use of biological fertilization and the medium level of chemical fertilization. Interestingly, with decreasing the rate of chemical N fertilization, rice nutrient use efficiency increased indicating how biological fertilization can be efficient in providing plants with its essential nutrients such as N. However, the highest rate of soil and straw nutrient concentration was related to the combined use of biologicalfertilization and the highest rate of chemical fertilization. We conclude that biological fertilizer, （mycorrhizal fungi and H. seropedicae） can significantly improve wetland rice growth and yield （resulting in the decreased rate of chemical fertilizer）, espe- cially if combined with appropriate rate of chemical fertilization, by enhancing nutrient uptake （fortification） and root growth.

Effect of Fertilization on Tomato Bacterial Wilt Biocontrolling and Soil Health Restoration Using FAME Analysis

The effect of applying biological organic fertilizer(BOF)on bacterial wilt incidence of tomato and soil microbial community under continuous cropping was studied. The results showed that all the tomatoes were infected by bacterial wilt in the control. The infection rates of tomatoes in the treatments with un-composted BOF and decomposed BOF were 55 and 50% respectively. Fatty acid methyl esters(FAME)analysis indicated soil microbial community changed sensitively after applying BOF. Soil FAME total content, relative fungi content and ratio of fungi to bacteria were significantly increased in both BOF treatments. The soil odd-number fatty acid proportion changed after applying BOF, aC15 : 0, iC17 : 0 decreased, while cyC17 : 0 increased in soil odd-number fatty acid proportion. BOF application would strengthen soil health and disease suppression. The content of C16 : 1 11c in soil microbial community was obviously increased after decomposed BOF application. It indicated that the growth of AM fungi could be enhanced with decomposed BOF application. FAME microbial biomarkers could be used for an indicator of soil health and disease suppression. Odd-number fatty acid proportion was a sensitive indicator of the effect of applying un-composted and decomposed BOF respectively on soil health regulating.

Effect of biochar applied with plant growth-promoting rhizobacteria(PGPR)on soil microbial community composition and nitrogen utilization in tomato

Plant growth-promoting rhizobacteria(PGPR)represent an important microbial community group and have beneficial effects on plant growth and development.A pot experiment was conducted to study the effect of biochar applied with PGPR on the soil microbial community composition and nitrogen use efficiency(NUE)of tomato,which could provide a theoretical basis for rational fertilization.Six treatments were designed:no nitrogen(N),PGPR,or biochar control(CK);biochar without N or PGPR(BCK);N without PGPR or biochar(U);N and PGPR without biochar(UP);N and biochar without PGPR(UB);and N,PGPR,and biochar(UBP).The tomato yield in the UP treatment was 9.09% lower than that in the U treatment,whereas that in the UB treatment was 19.93% higher than that in the U treatment.The tomato yield in the UBP treatment was 32.45%,45.69%,and 10.44% higher than those in the U,UP,and UB treatments,respectively.Biochar combined with PGPR increased the relative abundance of Nitrospira and Bradyrhizobium in the soil.At the tomato maturity stage,the soil NO_(3)^(-)-N content in the UBP treatment was 87.12%,88.12%,and 31.04% higher than those in the U,UP,and UB treatments,respectively.The NUE in the UP treatment was 4.03% lower than that in the U treatment,and that in the UBP treatment was 13.63%,17.66%,and 10.77% higher than those in the U,UP,and UB treatments,respectively.This study showed that biochar combined with PGPR can improve soil microbial community structure and increase the NUE of tomato.

Influences of Biochar and Biochar-Mineral Complex on Mycorrhizal Colonisation and Nutrition of Wheat and Sorghum

The high price of synthetic fertilisers and the price barrier for biochar as a soil amendment have encouraged the exploration of using biochar in fertiliser replacement formulations. Biochars coupled with fertilisers can be applied at lower application rates to achieve benefits in plant growth and nutrition, as well as soil biological fertility. It is necessary to evaluate the use of biochar as a fertiliser substitute. Therefore, this study investigated the comparative influences of biochars, including Acacia saligna （AS）, Simcoa jarrah （S J） and Wundowie jarrah （W J）, mineral fertiliser with microbes （MF ＋ M）, biochar-mineral complex （BMC） and their combination on mycorrhizal colonisation, growth and nutrition of wheat in a glasshouse experiment and sorghum in field conditions. BMC ＋ MF ＋ M treatment produced higher mycorrhizal colonisation than MF ＋ M alone, indicating that BMC had a significant role in increasing mycorrhizal colonisation. SJ （treated with acetic acid） and MF ＋ M treatments, as well as AS ＋ MF ＋ M application, showed similar effects on mycorrhizal colonisation, but lower colonisation than the BMC ＋ MF ＋ M treatment. Ovcrall~ the BMC ＋ MF ＋ M treatment supported the maximum shoot, root and total plant dry weight followed by AS ＋ MF ＋ M and WJ ＋ MF ＋ M. The MF ＋ M treatment had the maximum shoot N and K concentrations, while BMC ＋ MF ＋ M application had the maximum shoot P concentration. AS ＋ MF -4- M and WJ ＋ MF ＋ M treatments supported the maximum N uptake by wheat shoots, while BMC ＋ MF ＋ M supported the maximum P uptake. The results showed that biochars and BMCs could increase mycorrhizal colonisation, plant growth and nutrient uptake of wheat, particularly N, P, K, S and Zn. The field experiment confirmed that BMC application at a rate of 300 kg ha-1 could increase the yield of irrigated sorghum on a loam soil and provide better applied P use efficiency compared to a water-soluble fertiliser alone. These results indicated that biochar-based fertilisers might increase the resilience and sustainability of dryland cropping in environments such as in Western Australia and warrant further field evaluation.