IN VITRO ANTI-HEPATOMA EFFECTS OF MONOCYTES AND KUPFFER CELLS ISOLATED FROM HEPATOMA PATIENTS AFTER TREATMENT WITH BIOLOGICAL IMMUNE STIMULANTS

Monocytes (MC), lymphocytes (LC) and Kupffer cells (KC) were isolated respectively from blood and surgical liver samples of patients suffering from he-patocellular carcinoma (HCC). 13 patients were given BCG, mixed bacterium vaccine (MBV) and human white blood cell interferon (IFN), the other 3 patients were not treated with any biological immune stimulants (BIS) and served as controls. The cytosta-tic and cytotoxic effects of MC and KC on human hepatoma SMMC-7721 (TC) were assayed in vitro and the numbers of T total (Tt), T helper (Th) and T suppressor (Ts) cells were counted using CD monoclonal antibody immunofluorescence. The results were as follows: (1) On the 7th day after the first administration of BIS, the cytostatic and cytotoxic effects of MC on TC showed obvious increase over pre-administration. The activity of BIS was 1 ?5 times as high as that in the controls. (2) After 3 administrations, the cytostatic effect of MC on TC increased to the normal level (84%), while the controls remained as before (45%). (3) On the 7th day after first administration, cytostatic and cytotoxic effects of KC on TC were 0.5 and 1 times higher respectively than those of the controls. (4) The numbers of Tt and Th of patients given BIS increased continuously; on the contrary Ts decreased in number. These results indicate that combined use of BCG, MBV and IFN can actively enhance the immune anti-hepatoma function of patients suffering from HCC.

Application of Clinical Nursing Pathway Combined with Cluster Nursing Mode in Biologic Treatment of Rheumatoid Immune Disease Patients

Objective: To investigate the effect of clinical nursing pathway (CNP) combined with cluster nursing mode in intravenous biologic treatment of rheumatoid immune disease patients. Methods: Convenience sampling method was used to select inpatients receiving biologics treatment in Rheumatology and Immunology Department of a grade A hospital in Jingzhou city from May 2020 to April 2022. 75 patients from May 2020 to April 2021 were selected as the control group, and 75 patients from May 2021 to April 2022 were selected as the observation group. The control group was given routine care. The observation group was treated with CNP combined with cluster nursing mode on the basis of the control group, and the incidences of adverse infusion reactions, total treatment time, patient satisfaction and nurse satisfaction were compared between the two groups. Results: There were statistically significant differences between the two groups after intervention (P Conclusion: Using CNP combined with cluster nursing mode is beneficial to reduce the incidence of adverse infusion reactions in patients, shorten the total treatment time, and improve the satisfaction of patients and nurses.

Biological therapy for ulcerative colitis:An update

Of the diverse biological agents used for patients with ulcerative colitis, the anti-tumor necrosis factor-α agents infliximab and adalimumab have been used in large-scale clinical trials and are currently widely used in the treatment of inflammatory bowel disease patients. Recent studies have indicated that golimumab, oral tofacitinib and vedolizumab reportedly achieved good clinical response and remission rates in ulcerative colitis patients. Thus, we believe that the detailed investigation of various studies on clinical trials may provide important information for the selection of appropriate biological agents, and therefore, we have extensively reviewed such trials in the present study.

Damage on intestinal barrier function and microbial detoxification of deoxynivalenol:A review

Deoxynivalenol(DON)is a mycotoxin that is produced by various species of Fusarium and is ubiquitous in food and feed.At low concentrations,it can cause metabolic disorders in animals and humans and,at high concentrations,it can lead to pathological changes in the body.The impact of DON on human/animal health and animal productivity has thus attracted a great deal of attention around the world.DON causes severe damage to the intestine,including compromised intestinal barrier,mucosal damage,weakened immune function,and alterations in gut microbiota composition.These effects exacerbate intestinal infections and inflammation in livestock and poultry,posing adverse effects on overall health.Furthermore,research into biological methods for DON detoxification is a crucial avenue for future studies.This includes the utilization of adsorption,enzymatic degradation,and other biological approaches to mitigate DON's impact,offering new strategies for prevention and treatment of DON-induced diseases.Future research will focus on identifying highly efficient detoxifying microorganisms or enzymes to reduce DON levels in food and feed,thereby mitigating its risks to both animals and human health.

手掌BIS的特征模型及特征提取算法研究

针对现有的生物特征识别技术在特征提取、特征选择、模式分类及身份识别中,存在获取的特征值不是最优解、身份识别误判率高等问题。首次通过手掌生物电阻抗频谱(BIS)数据,建立了一种BIS数据的有效特征椭圆模型(EM);并融合免疫克隆技术和最小二乘法,提出了一种手掌电阻抗频谱BIS特征选择新算法。实验表明:该算法不仅能充分挖掘免疫克隆快速收敛到全局最优解的分类特性,而且能有效实现了EM特征的提取与选择,使身份识别的正确率提高到95%。因此,所提出的EM特征模型与特征选择算法在信息安全、电子商务等领域应用中具有重要的理论和实用价值。

Prognostic potential of an immune score based on the density of CD8＋ T cells, CD20＋ B cells, and CD33＋/p-STAT1＋ double-positive cells and HMGB1 expression within cancer nests in stage ⅢA gastric cancer patients

Objoctive： There is heterogeneity in the prognosis of gastric cancers staged according to the tumornodes-metastasis （TNM） system. This study evaluated the prognostic potential of an immune score system to supplement the TNM staging system. Mothodsg An immunohistochemical analysis was conducted to assess the density of T cells, B cells, and myeloid-derived suppressor cells （MDSCs） in cancer tissues from 100 stage IIIA gastric cancer patients; the expression of the high-mobility group protein B1 （HMGB1） was also evaluated in cancer cells. The relationship between the overall survival （OS）, disease-free survival （DFS）, and immunological parameters was analyzed.Results： An immune score system was compiled based on the prognostic role of the density ofT cells, B cells, MDSCs, and the expression of HMGB1 in cancer tissues. The median 5-year survival of this group of patient was 32%. However, the 5-year survival rates of 80.0%, 51.7%, 0%, 5.8%, and 0% varied among the patients with an immune score of 4 to those with an immune score of 0 based on the immune score system, respectively. Similarly, differences in DFS rates were observed among the immune score subgroups. Concluslons： An immune score system could effectively identify the prognostic heterogeneity within stage IliA gastric cancer patients, implying that this immune score system may potentially supplement the TNM staging system, and help in identifying a more homogeneous group of patients who on the basis of prognosis can undergo adjuvant therapy.

Event-driven Dynamic and Intelligent Scheduling for Agile Manufacturing Based on Immune Mechanism and Expert System

Based on the biological immune concept, immune response mechanism and expert system, a dynamic and intelligent scheduling model toward the disturbance of the production such as machine fault,task insert and cancel etc. Is proposed. The antibody generation method based on the sequence constraints and the coding rule of antibody for the machining procedure is also presented. Using the heuristic antibody generation method based on the physiology immune mechanism, the validity of the scheduling optimization is improved, and based on the immune and expert system under the event-driven constraints, not only Job-shop scheduling problem with multi-objective can be solved, but also the disturbance of the production be handled rapidly. A case of the job-shop scheduling is studied and dynamic optimal solutions with multi-objective function for agile manufacturing are obtained in this paper. And the event-driven dynamic rescheduling result is compared with right-shift rescheduling and total rescheduling.

白头翁汤对溃疡性结肠炎肠黏膜屏障作用机制

近年来溃疡性结肠炎临床发病率不断上升,现代医学对其治疗手段较为局限,并且存在较大的不良反应,疗效难以维持等诸多问题。中医学以其特有的理论体系和治疗模式在治疗本病时具有独特优势,可以有效改变现代医学的治疗困境。白头翁汤作为中医典籍名方,对溃疡性结肠炎的临床治疗效果显著。在临床上广泛运用于溃疡性结肠炎的治疗,然而关于其具体治疗机制尚无定论。系统论述白头翁汤对溃疡性结肠炎肠黏膜屏障作用机制有利于指导临床诊疗工作。

铁死亡相关基因表达与结直肠癌患者预后的关系

目的探寻结直肠癌(CRC)患者铁死亡相关基因的差异表达情况,分析这些基因的表达与患者预后的关系。方法从基因表达谱数据库中选取CRC差异表达基因数据集GSE32323、GSE21510和GSE934及铁死亡相关基因集为研究对象,筛选CRC患者差异表达的铁死亡相关基因,并对筛选的共差异表达基因进行蛋白-蛋白相互作用(PPI)网络构建、基因本体论(GO)功能和京都基因与基因组百科全书(KEGG)信号通路富集分析,估算免疫细胞浸润与基因表达水平的相关性。根据共差异表达基因表达水平分为高、低表达组,比较两组患者总生存率和无病生存率。回顾性分析2018年1月至2023年12月丽水市人民医院手术切除的44例CRC组织石蜡切片,采用免疫组化染色检测患者癌组织中共差异表达基因蛋白表达水平,并分析其与患者临床病理特征的关系。结果4个数据集中有共差异表达基因3个,分别为MYC、电压依赖阴离子通道蛋白3(VDAC3)基因和缺氧诱导脂滴相关基因(HILPDA)。PPI分析显示,3个蛋白共有蛋白节点33个,节点间相互作用219种,平均聚类指数为0.68。GO功能和KEGG信号通路富集分析显示,3个基因功能主要富集于转录调控、DNA模板、核质及转录因子结合;信号通路主要富集于癌症、细胞周期和铁死亡。VDAC3基因高表达组患者无进展生存率和总生存率均显著高于低表达组(均P<0.05),而MYC、HIL-PDA高、低表达组患者总生存率和无病生存率比较,差异均无统计学意义(均P>0.05)。结肠癌患者中,VDAC3表达水平与B细胞、CD8^(+)T细胞、CD4^(+)T细胞和中性粒细胞浸润有关(均P<0.05);直肠癌患者中,则与CD8^(+)T细胞、CD4^(+)T细胞浸润有关(均P<0.05)。免疫组化显示VDAC3主要在细胞质中阳性表达。44例CRC患者VDAC3阳性表达20例(45.5%),VDAC3阳性表达组和阴性表达组患者肿瘤分化程度、血管侵犯比较,差异均有统计学意义(均P<0.05)。结论MYC、VDAC3基因和HILPDA为CRC患者差异表达的铁死亡相关基因,VDAC3低表达与CRC患者预后不良有关。

中草药添加剂的生物学功能及其在反刍动物生产和疾病防治中的应用

中草药具有来源广泛、取材方便、低残留、无耐药性等特点,是一类可以替代抗生素的绿色饲料添加剂。中草药中含有有机酸、生物碱、黄酮类、挥发油等活性成分,在抗氧化、抑菌、消炎、增强免疫、抗肿瘤等方面具有较好的生理作用。中草药添加剂可以促进反刍动物机体生长发育,促进机体消化吸收,改善肠道健康,增强免疫及抗氧化性能,预防和治疗疾病,在反刍动物生产中具有较好的应用前景。文章综述中草药添加剂的生物学功能,论述其在反刍动物生产和疾病防治的应用,为中草药添加剂在反刍动物无抗健康养殖中的高效利用提供参考。

结核分枝杆菌潜伏感染者外周血单个核细胞转录组学研究

目的:通过对比分析结核分枝杆菌潜伏感染(latent tuberculosis infection,LTBI)者和健康人的外周血单个核细胞(peripheral blood mononuclear cells,PBMC)经结核特异性抗原刺激后的转录组,寻找LTBI人群中结核抗原特异的免疫应答重要基因及可能发挥功能的信号通路,绘制LTBI基因表达谱。方法:应用微阵列芯片检测2009年12月在首都医科大学附属北京胸科医院招募的4例LTBI者(LTBI组)和4名健康人(健康对照组)经结核抗原刺激后的PBMC转录组,并对部分差异基因进行qPCR检测以验证芯片数据。应用加权基因共表达网络分析(weighted gene co-expression network analysis,WGCNA)筛选与LTBI最相关模块,并对模块中的基因分别进行基因本体论(geneontology,GO)分析、京都基因与基因组百科全书(Kyoto encylopedia of genes and genomes,KEGG)通路分析。通过基因集富集分析(gene set enrichment analysis,GSEA)从全转录组水平筛选信号通路。通过免疫浸润分析寻找与LTBI相关的免疫细胞。通过STRING数据库蛋白相互作用(protein-protein interaction,PPI)网络分析绘制两模块中差异基因相互作用网络图。结果:LTBI组与健康对照组间共发现393个差异表达基因(差异倍数>2或<0.5,P<0.05),LTBI中112个表达下调,281个表达上调。针对10个差异基因的实时荧光定量PCR(quantitative real-time PCR,qPCR)检测证实基因表达趋势与芯片数据一致,即CAMK1G、IL2、SPINK1、SUCNR1、HCAR3、IL18BP、CHI3L1、TNF均上调;RASGRP2、TPM2均下调。通过WGCNA分析确定与LTBI最相关的正相关蓝色模块(cor=0.88,P=0.004)和负相关蓝绿色模块(cor=―0.93,P=0.001)。对上述模块内基因进行KEGG富集分析,并行基于全转录组的GSEA分析,发现趋化因子信号通路(P<0.001)和凋亡信号通路(P=0.003)在两种分析中均明显富集。对两模块中的差异基因通过STRING数据库构建PPI网络图,通过内置插件计算得到每个模块中的前10个关键基因,即蓝色模块中的PTPRC、CD40、IL10、IRF8、CCR1、CD80、TLR8、CLEC7A、CD83和CD274;蓝绿色模块中的TNF、ICAM1、TNFRSF4、HAVCR2、CD276、CCL4、CD33、IL1RN、NCF1和FGR。免疫浸润分析发现,M1型巨噬细胞(P=0.029)和M2型巨噬细胞(P=0.001)等固有免疫细胞的比例在LTBI组和健康对照组间存在明显差异。结论:经结核特异性抗原刺激后的PBMC转录组在LTBI人群和健康人群间存在明显差异,这些差异基因主要聚集于凋亡信号通路和趋化因子信号通路,其中巨噬细胞介导的免疫应答在其中发挥了重要作用,揭示了固有免疫应答在LTBI中的重要作用。

尖孢镰刀菌对秀丽隐杆线虫生物学特性及表达转录组的影响

【目的】探讨尖孢镰刀菌Fusarium oxvsporum对秀丽隐杆线虫Caenorhabditis elegans生物学特征的影响,明确线虫响应尖孢镰刀菌侵染的关键通路。【方法】将尖孢镰刀菌活性孢子和灭活孢子分别与秀丽隐杆线虫共培养,测定C.elegans寿命、体长、运动能力、繁殖力以及咽泵运动速率等基本生物学特征;进行全基因组测序,通过GO和KEGG分析明确线虫响应尖孢镰刀菌侵染的关键通路和生物学功能。【结果】尖孢镰刀菌活性分生孢子与线虫共培养可导致线虫寿命下降,虫体显著缩短,线虫繁殖力显著降低;但共培养并未对线虫的咽泵运动速率及运动能力产生明显影响。GO分析发现,差异表达基因富集在先天免疫反应和氧化还原酶活性等生物学功能方面;KEGG分析发现,差异表达基因富集在药物代谢−细胞色素P450、代谢外源性物质的细胞色素P450和脂肪酸代谢等通路。【结论】尖孢镰刀菌对秀丽隐杆线虫的基本生命活动有不利影响,这为深入探讨线虫响应尖孢镰刀菌的天然免疫机制及与植物免疫的相似性,从而寻找植物相关抗病基因奠定了基础。

凝结芽孢杆菌对畜禽肠道健康调控作用的研究进展

凝结芽孢杆菌是一种新型的饲用益生菌,不仅具有乳酸菌和双歧杆菌的益生作用,还具有芽孢杆菌的耐高温、耐胃酸和耐胆盐等抗逆性,以及易培养、易储存的生物学特性。诸多研究表明,凝结芽孢杆菌具有平衡肠道菌群结构、改善肠道形态、促进消化吸收、增强肠道免疫等多种作用。本文主要综述了凝结芽孢杆菌对畜禽肠道健康的调控作用,旨在为凝结芽孢杆菌在畜禽绿色养殖和生产的进一步应用提供理论参考。

生物介质传质系数Bi-G模型可靠性实验测定

为验证分析Biot数-迟滞因子(Bi-G)关系式估算生物介质热风干燥的对流传质系数与水分扩散系数可靠性,首先采用指数函数对胡萝卜切片干燥实验数据进行拟合,利用Bi-G关系式估算其对流传质系数与水分扩散系数;然后采用该估算值,通过生物介质内部水分扩散遵循Fick第二扩散定律、生物介质-热空气间遵循对流传质的干燥模型,对热风对流干燥胡萝卜切片水分变化进行了数值模拟计算,并对数值模拟计算值与干燥实验数据进行了比较。结果表明:指数函数对干燥实验数据拟合曲线与干燥实验数据吻合不是Bi-G关系式精确估算对流传质系数与水分扩散系数的充分条件,Bi-G关系式不能用来精确估算对流传质系数与水分扩散系数。

抗菌肽种类、生物学功能及在畜禽生产中的应用

抗菌肽因具有抗菌、抗病毒、抗肿瘤细胞和免疫调节等生物学功能,在人类抗肿瘤药物开发、食品加工和畜牧兽医领域受到广泛关注。在饲料中,添加抗菌肽可以提高畜禽生产性能,增强机体免疫力,表现出替代抗生素的良好潜力。本文综述了抗菌肽的种类、生物学功能及在畜禽生产中的应用,旨在全面、深入了解抗菌肽的作用,为开发新型绿色饲料添加剂提供参考,以推动抗菌肽在畜牧领域的广泛应用。

至真方固体双向发酵工艺的建立及其药效评价

目的以中药固体双向发酵方法,建立至真方的新型发酵工艺。方法①通过文献研究拟定候选发酵菌种。对候选发酵菌种进行平板混合实验,考察各菌种间生长兼容性。采用CCK-8细胞增殖抑制实验,比较不同发酵条件下所得产物对人结直肠癌细胞(HCT-8)的半数抑制浓度(IC50),评判发酵体系的优劣,从而确定发酵菌种、发酵时长等关键发酵参数,建立发酵工艺。②通过HCT-8细胞增殖抑制率,以及巨噬细胞(RAW264.7)NO释放率和吞噬活性测定,比较至真方发酵前后的体外药效差异。结果黑曲霉和酵母菌为最优发酵菌种组合,4 d为最优发酵时长。在此发酵条件下,发酵组对HCT-8细胞的IC50为67.41μg·mL^(-1),生药组对HCT-8细胞的IC50为1032μg·mL^(-1);与生药组比较,发酵组能显著抑制RAW264.7释放炎症因子NO,并显著提升其吞噬活性。结论该研究建立了至真方固体双向发酵新工艺。在该发酵体系下,至真方的体外抗结直肠癌活性、抗炎活性和促免疫活性得到显著提升。

杨梅小环腹瘿蜂生物学特性及其对寄主免疫的影响

杨梅小环腹瘿蜂Leptopilina myrica是一种于浙江省诱捕得到,能高效寄生果蝇幼虫的内寄生蜂,目前国内外对该物种的报道较少。本研究运用形态成像,血细胞计数和实时荧光定量PCR等方法对杨梅小环腹瘿蜂的生物学特性及其寄生对寄主免疫反应的影响进行研究。结果表明,杨梅小环腹瘿蜂在寄主体内完成卵期、幼虫期和蛹期三个阶段。在实验室条件下,杨梅小环腹瘿蜂能高效寄生包括斑翅果蝇在内的多种果蝇属害虫;其对黑腹果蝇Drosophila melanogaster的寄生率和出蜂率相对较高,分别为87.3%和70.6%。此外,杨梅小环腹瘿蜂寄生会引起黑腹果蝇寄主强烈的免疫反应:被寄生后的寄主血细胞数量明显增加,并在寄生48 h后诱导产生大量的薄层细胞参与包囊反应;寄生后的寄主体内Imd、Toll以及PO等免疫通路中主要基因的转录水平显著上升。面对黑腹果蝇寄主的免疫反应,杨梅小环腹瘿蜂通过将卵粘附于肠道等寄主组织的方式逃避寄主免疫,从而成功寄生。本研究揭示了杨梅小环腹瘿蜂成功寄生果蝇类害虫的机制,为害虫生物防治提供重要的知识储备。

创伤后脓毒症关键基因筛选及其与免疫基因相关性的生物信息学分析

目的基于生物信息学方法分析创伤后脓毒症的关键基因及其与免疫基因相关性。方法从基因表达数据库(geneexpressionomnibus,GEO)中下载GSE12624数据集,筛选创伤后脓毒症患者外周血中差异基因,对差异基因进行基因本体(gene ontology,GO)和京都基因与基因组百科全书(Kyotoencyclopediaofgenesandgenomes,KEGG)富集分析,使用String、Cytoscape构建蛋白质-蛋白质互作网络(Protein-Protein Interaction Networks,PPI)及基于Cytohubba插件选出关键基因,通过受试者操作特征曲线的曲线下面积选出枢纽基因,验证其在创伤后脓毒症中的表达情况,通过基因集富集分析(gene set enrichment analysis,GSEA)、基因集变异分析(gene set variation analysis,GSVA)、免疫浸润分析以及分析其与免疫基因、免疫细胞相关性。结果在GSE12624数据集中获得362个差异基因,GO富集分析示差异基因参与信号转导、细胞凋亡、炎症反应相关的生物学过程,KEGG富集分析示,差异基因主要富集在补体和凝血级联通路、细胞周期等信号通路;选出曲线下面积最大值(0.815)的基因AURKB作为创伤后脓毒症的枢纽基因,其在创伤后脓毒症中高表达;通过GSEA富集分析显示AURKB高表达组主要富集到代谢、细胞周期、P53信号通路等相关的基因集,GSVA富集分析显示AURKB高表达组主要集到糖胺聚糖降解、花生四烯酸代谢通路、焦点黏附通路等相关途径;AURKB的表达与免疫基因(IL21R、CXCL10)的表达、免疫细胞(初始CD4+T细胞、滤泡辅助性T细胞)浸润呈正相关。结论AURKB在创伤后脓毒症中表达上调,参与创伤后脓毒症的免疫调节通路,可作为创伤后脓毒症早期预警的分子标志物及干预潜在靶标。

腺病毒主要蛋白质的生物学功能

腺病毒(Adenovirus,ADV)是无囊膜包裹的线性双链DNA病毒,致病性强,传播范围广,可感染多种动物。自1953年研究人员首次分离ADV以来,已发现100多种血清型。ADV的主要蛋白包括纤突蛋白(Fiber)、五邻体蛋白(Penton)、六邻体蛋白(Hexon)、Knob、E1B-55K、Ⅸ、E1A、100K和E3等。ADV识别受体、入侵细胞、脱衣壳、基因组入核、组装和释放均由上述蛋白质调控。文章综述了ADV主要蛋白质的研究进展,旨在为进一步探究ADV致病机制和制订防控策略提供参考。

右美托咪定通过cGAS-STING通路介导的免疫调控机制对胃癌细胞恶性生物学行为的影响

目的:探究右美托咪定(DEX)通过环磷酸鸟苷-磷酸腺苷合酶-干扰素基因刺激因子(cGAS-STING)通路介导的免疫调控机制对胃癌(GC)细胞恶性生物学行为的影响。方法:将GC细胞株MGC-803随机分为对照组(Control组,空白培养基处理)、DEX低浓度组(DEX-L组,1 ng/ml)、DEX中浓度组(DEX-M组,10 ng/ml)、DEX高浓度组(DEX-H组,100 ng/ml)和DEX高浓度+cGAS抑制剂RU.521组(DEX-H+RU.521组,100 ng/ml DEX+1.0μmol/L RU.521)。CCK-8法检测细胞增殖情况。细胞划痕实验检测各组细胞的迁移能力。Transwell实验检测各组细胞的侵袭能力。流式细胞术检测细胞凋亡率。ELISA检测细胞中IL-2、干扰素-γ(IFN-γ)、肿瘤坏死因子α(TNF-α)水平。实时荧光定量PCR(RT-qPCR)法检测细胞cGAS、STING、Ⅰ型干扰素(IFN-Ⅰ)mRNA的表达水平。Western blot检测细胞cGAS、STING、Bax、细胞周期蛋白D1(CyclinD1)、基质金属蛋白酶9(MMP9)、N钙黏蛋白(N-cadherin)、波形蛋白(Vimentin)、E钙黏蛋白(E-cadherin)、Caspase3、Caspase8及其剪切型蛋白表达和TANK结合激酶1(TBK1)、干扰素调节因子3(IRF3)磷酸化水平。结果:与Control组相比,DEX-M组、DEX-H组MGC-803细胞迁移率、细胞侵袭数目、TNF-α水平、CyclinD1、MMP9、N-cadherin、Vimentin蛋白表达显著下降(P<0.05),生长抑制率(48 h、72 h)、细胞凋亡率、IL-2、IFN-γ、Bax、E-cadherin、Cleaved Caspase3、Cleaved Caspase8蛋白表达水平、cGAS、STING、IFN-ⅠmRNA水平和蛋白表达水平、TBK1和IRF3磷酸化水平显著上升(P<0.05)。RU.521减弱了DEX对GC细胞增殖、迁移和侵袭的抑制作用和诱导细胞凋亡的能力,减轻了对免疫功能的改善作用。结论:DEX可能通过激活cGAS-STING通路介导的免疫调控,抑制GC细胞增殖、迁移和侵袭,诱导GC细胞凋亡。