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Stimulated Raman scattering microscopy on biological cellular machinery
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作者 Jing Huang Minbiao Ji 《Journal of Innovative Optical Health Sciences》 SCIE EI CAS CSCD 2023年第2期75-91,共17页
Beneting from the developments of advanced optical microscopy techniques,the mysteries of biological functions at the cellular and subcellular levels have been continuously revealed.Stimulated Raman scattering(SRS)mic... Beneting from the developments of advanced optical microscopy techniques,the mysteries of biological functions at the cellular and subcellular levels have been continuously revealed.Stimulated Raman scattering(SRS)microscopy is a rapidly growing technique that has attracted broad attentions and become a powerful tool for biology and biomedicine,largely thanks to its chemical specicity,high sensitivity and fast image speed.This review paper introduces the principles of SRS,discusses the technical developments and implementations of SRS microscopy,then highlights and summarizes its applications on biological cellular machinery andnally shares our visions of potential breakthroughs in the future. 展开更多
关键词 Nonlinear optical microscopy vibrational spectral imaging coherent Raman scattering biological cells
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Natural nitrogen-doped multiporous carbon from biological cells as sulfur stabilizers for lithium-sulfur batteries 被引量:1
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作者 Yan-Ping Xie Hong-Wei Cheng +5 位作者 Wei Chai Hong Yue Xuan Zhang Jian-Hui Fang Hong-Bin Zhao Jia-Qiang Xu 《Chinese Chemical Letters》 SCIE CAS CSCD 2017年第4期738-742,共5页
In this context,we firstly synthesized a novel nitrogen-doped multiporous carbon material from renewable biological cells through a facile chemical activation with K;CO;.After sulfur impregnation,the carbon/sulfur com... In this context,we firstly synthesized a novel nitrogen-doped multiporous carbon material from renewable biological cells through a facile chemical activation with K;CO;.After sulfur impregnation,the carbon/sulfur composite achieved a sulfur content of about 67 wt%.The C/S composite as the cathode of lithium-sulfur batteries exhibited a discharge capacity of 1410 mAh/g and good capacity retention of912 mAh/g at 0.1C.These outstanding results were attributed to the synergy effect of microporous carbon and natural doping nitrogen atoms.We believe that the facile approach for the synthesis of nitrogen-doped multiporous carbon from the low-cost and sustainable biological resources will not only be applied in lithium-sulfur batteries,but also in other electrode materials. 展开更多
关键词 biological cell Multiporous carbon Nitrogen-doped Lithium-sulfur batteries
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In vitro growth, differentiation and biological characteristics of neural stem cells 被引量:20
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作者 Meijiang Yun Lianzhong Wang +1 位作者 Yongcai Wang Xiaolian Jiang 《Neural Regeneration Research》 SCIE CAS CSCD 2006年第4期364-367,共4页
OBJECTIVE: To summarize the biological characteristics of neural stem cells, and the separation, purification. differentiation and source of neural stem cells. DATA SOURCES : An online search of Pubmed database was ... OBJECTIVE: To summarize the biological characteristics of neural stem cells, and the separation, purification. differentiation and source of neural stem cells. DATA SOURCES : An online search of Pubmed database was undertaken to identify English articles about the growth of neural stem cells in vitro published from January 2000 to October 2006 by using the keywords of "neural stem cells, bone marrow mesenchymal stem cells (BMSCs), umbilical cord blood stem cells, embryonic stem cells (ESC), separation methods, neural growth factor". And relevant articles published in IEEE/IEE Electronic Library (IEL) database, Springer Link database and Kluwer Online Journals were also searched, Chinese relevant articles published between January 2000 to October 2006 were searched with the same keywords in Chinese in Chinese journal full-text database. STUDY SELECTION : The articles were primarily screened, and then the full-texts were searched. Inclusive criteria: (1) Articles relevant to the biological characteristics and classification of neural stem cells; (2) Articles about the source, separation and differentiation of the ESCs, BMSCs and umbilical cord blood stem cells. The repetitive studies and reviews were excluded. DATA EXTRACTION : Thirty articles were selected from 203 relevant articles according to the inclusive criteria Articles were excluded because of repetition and reviews. DATA SYNTHESES : Neural stem cells have the ability of self-renewing and high differentiation, and they are obtained from ESCs, nerve tissue, nerve system, BMSCs and umbilical cord blood stem cells. ESCs can be separated by means of mechanical dissociation is better than that of the trypsin digestion, BMSCs by density gradient centrifuge separation, hemolysis, whole-blood culture, etc., and umbilical cord blood stem ceils by Ficoil density gradient centrifugation, hydroxyethyl starch (HES) centrifugation sedimentation, etc. Neural growth factor (NGF) and other factors play an important role in the growth of NSCs, such as transforming growth factor (TGF) is an important player in repairing organs, NGF accelerates the process of growth, insulin-like growth factor serves importantly in the differentiation of stem cells into neuron-like cells. CONCLUSION : As unipotent stem cells, NSCs have the abilities of self-renewal and potential of high differentiation. The method of mechanical dissociation is better than trypsin digestion in e separating ESCs. However, density gradient centrifuge separation is better than other methods in the separation of the BMSCs. NGF and other factors play an important role in the growth of NSCs. 展开更多
关键词 NSCS cell STEM differentiation and biological characteristics of neural stem cells In vitro growth BMSCS
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Improving cancer therapy by combining cell biological, physical, and molecular targeting strategies 被引量:1
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作者 Jac A. Nickoloff 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2013年第1期7-9,共3页
Successful cancer therapy depends on selective killing of tumor cells while sparing normal cells. Selectivity can be achieved through treatment strategies that target tumor cells. A recent report from the Li laborato... Successful cancer therapy depends on selective killing of tumor cells while sparing normal cells. Selectivity can be achieved through treatment strategies that target tumor cells. A recent report from the Li laboratory (1) describes an elegant strategy to selectively kill tumor cells by combining several targeting strategies based on cell biological, physical, and molecular (genetic) properties of tumor and normal cells that enhances tumor cell killing in vitro and in an in vivo tumor xenograft model. The idea of using a multiplex targeting approach is reminiscent of strategies in which several antibiotics are used to treat bacterial infections while minimizing the chance that rare antibiotic-resistant mutants will arise within a population. 展开更多
关键词 cell and molecular targeting strategies PHYSICAL Improving cancer therapy by combining cell biological DNA DSB
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Amyotrophic lateral sclerosis disease modifying therapeutics:a cell biological perspective
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作者 Bor Luen Tang 《Neural Regeneration Research》 SCIE CAS CSCD 2017年第3期407-408,共2页
Amyotrophic lateral sclerosis(ALS)is a progressively fatal neuromuscular disorder classically characterized by loss of upper and lower motor neurons from the cortex to the spinal cord Diagnosed patients have a media... Amyotrophic lateral sclerosis(ALS)is a progressively fatal neuromuscular disorder classically characterized by loss of upper and lower motor neurons from the cortex to the spinal cord Diagnosed patients have a median survival of about 3 years and death usually results from eventual respiratory failure. 展开更多
关键词 Amyotrophic lateral sclerosis disease modifying therapeutics:a cell biological perspective ALS cell
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IN VITRO ANTI-HEPATOMA EFFECTS OF MONOCYTES AND KUPFFER CELLS ISOLATED FROM HEPATOMA PATIENTS AFTER TREATMENT WITH BIOLOGICAL IMMUNE STIMULANTS
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作者 成令忠 韩伟 +4 位作者 钟翠平 顾云娣 赵岗 林芷英 汤钊猷 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1991年第2期37-40,共4页
Monocytes (MC), lymphocytes (LC) and Kupffer cells (KC) were isolated respectively from blood and surgical liver samples of patients suffering from he-patocellular carcinoma (HCC). 13 patients were given BCG, mixed ba... Monocytes (MC), lymphocytes (LC) and Kupffer cells (KC) were isolated respectively from blood and surgical liver samples of patients suffering from he-patocellular carcinoma (HCC). 13 patients were given BCG, mixed bacterium vaccine (MBV) and human white blood cell interferon (IFN), the other 3 patients were not treated with any biological immune stimulants (BIS) and served as controls. The cytosta-tic and cytotoxic effects of MC and KC on human hepatoma SMMC-7721 (TC) were assayed in vitro and the numbers of T total (Tt), T helper (Th) and T suppressor (Ts) cells were counted using CD monoclonal antibody immunofluorescence. The results were as follows: (1) On the 7th day after the first administration of BIS, the cytostatic and cytotoxic effects of MC on TC showed obvious increase over pre-administration. The activity of BIS was 1 ?5 times as high as that in the controls. (2) After 3 administrations, the cytostatic effect of MC on TC increased to the normal level (84%), while the controls remained as before (45%). (3) On the 7th day after first administration, cytostatic and cytotoxic effects of KC on TC were 0.5 and 1 times higher respectively than those of the controls. (4) The numbers of Tt and Th of patients given BIS increased continuously; on the contrary Ts decreased in number. These results indicate that combined use of BCG, MBV and IFN can actively enhance the immune anti-hepatoma function of patients suffering from HCC. 展开更多
关键词 BIS MC HCC IN VITRO ANTI-HEPATOMA EFFECTS OF MONOCYTES AND KUPFFER cellS ISOLATED FROM HEPATOMA PATIENTS AFTER TREATMENT WITH biological IMMUNE STIMULANTS
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ESTABLISHMENT OF A MAMMARY CANCER CELL LINE Ca 761-86 AND ITS BIOLOGICAL CHARACTERISTICS IN INBRED 615 MICE
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作者 程一棹 黄敏 +5 位作者 王明俭 郑怀祖 袁保和 许广源 程佳 钱振超 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1989年第4期43-48,共6页
A cell line designated as Ca 761-86 has been established from the solid mouse mammary cancer (Ca 761) by suspension culture. It has been passaged for more than 212 generations. Moderate round cells were predominant an... A cell line designated as Ca 761-86 has been established from the solid mouse mammary cancer (Ca 761) by suspension culture. It has been passaged for more than 212 generations. Moderate round cells were predominant and most of them were mononuclear. Some characteristics of malignant cells and A-type viral-like particles were observed by electron microscopy. The results of cytochemical studies (DNA, RNA, SDH, 5' AMPase, ACP etc.) were comparable to the ultramicroscopic results. It multiplied approximately 27.4 fold on day 5 with mitotic index reaching 1.8% on day 3. This cell line was a hyperdiploid with karyotype of 45 or 45, -2X, tril2, tri17, +M1-5. Cell agglutination was observed when treated with ConA (≥7 fig, ml). Spontaneous agglutination might also take place without adding any ConA. After 5×106 cells of Ca 761-86 suspension were transplanted into the normal inbred 615 mice by different ways (subcutan eous, intrafoot-pad or intraperitoneal), the transplan lability rate reached 100%. Spontaneous remission was never observed and its metastatic ability reserved. PPLO were not detected. Ca 761-86 may be of value for practical purposes. 展开更多
关键词 line ESTABLISHMENT OF A MAMMARY CANCER cell LINE Ca 761-86 AND ITS biological CHARACTERISTICS IN INBRED 615 MICE RER ConA CA
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Biological behaviors of two novel syngeneic human osteosarcoma cell lines
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作者 王晋 《外科研究与新技术》 2011年第2期123-124,共2页
Objective To characterize and compare the different biological behaviors of two novel human osteosarcoma cell lines,Zos and Zos-M,established respectively from the primary site and the skip metastasis of an osteosarco... Objective To characterize and compare the different biological behaviors of two novel human osteosarcoma cell lines,Zos and Zos-M,established respectively from the primary site and the skip metastasis of an osteosarcoma patient.Methods Two 展开更多
关键词 cell biological behaviors of two novel syngeneic human osteosarcoma cell lines
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FROM MOLECULAR RESPONSE TO CELL RESPONSE-Approaching the complexity of biological systems
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作者 Wang Kui(The School of Pharmaceutical, Beijing Medical University) 《Bulletin of the Chinese Academy of Sciences》 2000年第1期17-25,共9页
I. THE COMPLEXITY OFBIOLOGICAL RESPONSESFor an organism, to be living or notdepends on its response to foreign matters.Facing the increasing amount and diversi-ty of chemicals, natural and synthetic, tounderstand the ... I. THE COMPLEXITY OFBIOLOGICAL RESPONSESFor an organism, to be living or notdepends on its response to foreign matters.Facing the increasing amount and diversi-ty of chemicals, natural and synthetic, tounderstand the principles of the biologicalresponses becomes extremely importantin pursuing the way of rational utiliza-tion and governing the foreign matters.However, most biological responses aretoo complex to explore their nature. Forinstance, the risk to human beings andorganisms related to the application ofrare earths in agriculture, forestation, fish-ery and husbandry has been argued 展开更多
关键词 FROM MOLECULAR RESPONSE TO cell RESPONSE-Approaching the complexity of biological systems
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Biology-Physics the Missing Link?
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作者 Jean-Paul Auffray 《Journal of Modern Physics》 2014年第6期359-363,共5页
Writing in 1943, renowned Austrian physicist Edwin Schrodinger asked “What is Life?” thereby invigorating the debate which preoccupied biologists at the time. He proposed an answer to this question rooted in conside... Writing in 1943, renowned Austrian physicist Edwin Schrodinger asked “What is Life?” thereby invigorating the debate which preoccupied biologists at the time. He proposed an answer to this question rooted in considerations borrowed from Thermodynamics and Statistical Mechanics. To reveal the missing link in Biology-Physics, the present Note investigates an alternate answer in which dynamical action, rather than thermodynamics and energy, plays the fundamental role. It reviews in particular the process of biological cell replication which may be considered to define “Life” and might be the macroscopic manifestation of an underlying quantum physical process in which xons, conveyors of dynamical action, are the determining agents. 展开更多
关键词 SCHRODINGER Dynamical Action PLANCK Quantum Physics biological cells Molecules ELECTRONS Strings BRANES Xons
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Epigenome-Metabolome-Epigenome signaling cascade in cell biological processes 被引量:2
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作者 Linpeng Li Keshi Chen +2 位作者 Yi Wu Ge Xiang Xingguo Liu 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2022年第4期279-286,共8页
Cell fate determination as a fundamental question in cell biology has been extensively studied at different regulatory levels for many years.However,the mechanisms of multilevel regulation of cell fate determination r... Cell fate determination as a fundamental question in cell biology has been extensively studied at different regulatory levels for many years.However,the mechanisms of multilevel regulation of cell fate determination remain unclear.Recently,we have proposed an Epigenome-Metabolome-Epigenome(E-M-E)signaling cascade model to describe the cross-over cooperation during mouse somatic cell reprogramming.In this review,we summarize the broad roles of E-M-E signaling cascade in different cell biological processes,including cell differentiation and dedifferentiation,cell specialization,cell proliferation,and cell pathologic processes.Precise E-M-E signaling cascades are critical in these cell biological processes,and it is of worth to explore each step of E-M-E signaling cascade.E-M-E signaling cascade model sheds light on and may open a window to explore the mechanisms of multilevel regulation of cell biological processes. 展开更多
关键词 cell biological processes CHROMATIN EPIGENOME METABOLOME Signaling cascade
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Establishment and biological characteristics of human multiple myeloma cell line CZ-1 被引量:8
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作者 侯健 林法迎 +2 位作者 张波 张玲珍 丁思奇 《Chinese Medical Journal》 SCIE CAS CSCD 2004年第1期115-119,共5页
Background There were only 3 multiple myeloma (MM) cell lines established in China. In this study,we succeeded in establishing a novel MM cell line and analyzed its biological characteristics. Methods Mononuclear... Background There were only 3 multiple myeloma (MM) cell lines established in China. In this study,we succeeded in establishing a novel MM cell line and analyzed its biological characteristics. Methods Mononuclear cells isolated from the peripheral blood (PB) and bone marrow (BM) of a patient with advanced MM (λ light chain type) were cultured in medium. Cell morphology was analyzed by Wright-Giemsa-staining and cytochemical staining,immunophenotyping by flow cytometry and cytogenetic analysis by chromosome RHG-banding technique. Quantitative fluorescent polymerase chain reaction (PCR) was used to detect Epstein - Barr virus (EBV) DNA. Results The established cell line could survive and proliferate in the presence of feeder cells or conditioned medium. The cells secreted λ light chain and were negative for EBV. The Wright-Giemsa-staining showed typical plasmablast or plasma cell morphology. The cytochemical staining of the cells showed the following reactivity patterns: positive for acid phosphatase,negative for myeloperoxidase. The immunoprofile of the cells was concordant with that of MM cells: positive for CD_ 10 ,CD_ 28 ,CD_ 38 ,CD_ 138 ,CD_ 56 ,CD_ 49d ,CD_ 44 ,CD_ 54 and CD_ 58 ,negative for CD_ 19 , CD_ 40 ,CD_ 95 ,CD_ 95L ,CD_ 34 ,CD_2 and CD_5. The cytogenetic analysis showed complex chromosome abnormality of i (1q+),8q+,13q+,i (17q),i (18q) and +M. There was no difference in morphology,immunophenotype and cytogenetics between cells from PB and BM. Conclusions An MM cell line secreting λ light chain named CZ-1 was established. The cells from both PB and BM have the same biological characteristics. 展开更多
关键词 multiple myeloma·cell line·biological characteristics
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Biological character of human adipose-derived adult stem cells and influence of donor age on cell replication in culture 被引量:25
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作者 LEI Lei, LIAO WeiMing, SHENG PuYi, FU Ming, HE AiShan & HUANG Gang Department of Orthopedics, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China 《Science China(Life Sciences)》 SCIE CAS 2007年第3期320-328,共9页
To investigate the biological character of human adipose-derived adult stem cells (hADAS cells) when cultured in vitro and the relationship between hADAS cell’s replication activity and the donor’s age factor, and t... To investigate the biological character of human adipose-derived adult stem cells (hADAS cells) when cultured in vitro and the relationship between hADAS cell’s replication activity and the donor’s age factor, and to assess the stem cells as a new source for tissue engineering. hADAS cells are isolated from human adipose tissue of different age groups (from adolescents to olds: <20 years old, 21―40 years old, 41―60 years old and >61 years old groups). The protein markers (CD29, CD34, CD44, CD45, CD49d, HLA-DR, CD106) of hADAS cells were detected by flow cytometry (FCM) to identify the stem cell, and the cell cycle was examined for P20 hADAS cells to evaluate the safety of the subculture in vitro. The generative activity of hADAS cells in different age groups was also examined by MTT method. The formula “ log2T D = t logN t ? logN 0” was used to get the time doubling (TD) of the cells. The results showed that the cells kept heredity stabilization by chromosome analysis for at least 20 passages. The TD of these cells increased progressively by ageing, and the TD of the <20 years old group was lower than that of the >61 years old group (statistical analysis of variance (ANOVA), P=0.002, P<0.05). These find- ings suggested that a higher level of hADAS cells replication activity was found in the younger dona- tors, and they represent novel and valuable seed cells for studies of tissue engineering. 展开更多
关键词 biological character of human adipose-derived adult stem cells and influence of donor age on cell replication in culture cell STEM
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Peripheral Lymphocyte Subsets as a Marker of Parkinson's Disease in a Chinese Population 被引量:5
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作者 Luan Cen Chaohao Yang +13 位作者 Shuxuan Huang Miaomiao Zhou Xiaolu Tang Kaiping Li Wenyuan Guo Zhuohua Wu Mingshu Mo Yousheng Xiao Xiang Chen Xinling Yang Qinhui Huang Chaojun Chen Shaogang Qu Pingyi Xu 《Neuroscience Bulletin》 SCIE CAS CSCD 2017年第5期493-500,共8页
In this study, we conducted a clinical analysis of lymphocyte subtypes in 268 patients with Parkinson's disease (PD) to assess their clinical impact as a potential marker of advanced PD in Chinese patients. The par... In this study, we conducted a clinical analysis of lymphocyte subtypes in 268 patients with Parkinson's disease (PD) to assess their clinical impact as a potential marker of advanced PD in Chinese patients. The participants comprised 268 sporadic PD patients and 268 healthy controls. The numbers of natural killer (NK) cells and CD3+, CD3+CD4+, CD3+CD8+, and CD19+ lympho- cytes from peripheral blood were determined by immunos- taining and flow cytometric analysis and the percentages of these CD+ T cells were calculated. The ratio of regulatory T (Treg)/helper T 17 (Thl7) lymphocytes from 64 PD patients and 46 controls was determined by flow cytometric analysis. The results showed that the percentage of NK cells was higher in advanced PD patients than in controls (22.92% ± 10.08% versus 19.76% ±10.09%, P= 0.006), while CD3+ T cells are decreased (62.93% ±9.27% versus 65.75% ± 9.13%, P = 0.005). The percentage of CD19+ B cells in male patients was lower (P = 0.021) than in female patients, whereas NK cells were increased (P 〈 0.0001). The scores on the Unified Parkinson's Disease Rating Scale (UPDRS) and the Non-Motor Symptoms Scale in late-onset PD patients were significantly higher than those in earlyonset patients (P = 0.024 and P = 0.007, respectively). The percentage of CD19+ B cells in patients with UPDRS scores 〉24 was lower than in those with scores 〈24 (10.17% ± 4.19% versus 12.22% ± 5.39%, P = 0.009). In addition, the Treg/Th17 ratio in female patients was higher than that in female controls (13.88 ± 6.32 versus 9.94 ±4.06, P = 0.042). These results suggest that the percentages of NK cells, CD3+ T cells, and CD19+ B cells along with the Treg/Th17 ratio in peripheral blood may be used to predict the risk of PD in Chinese individuals and provide fresh avenues for novel diagnostic biomarkers and therapeutic designs. 展开更多
关键词 Parkinson's disease · biological marker ·Lymphatic cell
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