Taking the water ecosystem of Small Xingkai Lake as research object,through the survey and analysis of aquatic organism indicators,this paper established an ecosystem integrity evaluation system with the biological in...Taking the water ecosystem of Small Xingkai Lake as research object,through the survey and analysis of aquatic organism indicators,this paper established an ecosystem integrity evaluation system with the biological integrity as the criterion layer. Using the index of biological integrity and comprehensive health index,it evaluated the health status of water ecosystem of Small Xingkai Lake.展开更多
Dysregulated HGF/c-Met signalling has been associated with many human cancers,poor clinical outcomes,and even resistance acquisition to some approved targeted therapies.As such,c-Met kinase has emerged as an attractiv...Dysregulated HGF/c-Met signalling has been associated with many human cancers,poor clinical outcomes,and even resistance acquisition to some approved targeted therapies.As such,c-Met kinase has emerged as an attractive target for anticancer drug discovery.展开更多
The Hydrobios in Dongping Lake, Shandong Province, mainly includes phytoplankton, zooplankton, benthic animals, fishes and hydrophytic vascular plants. Nitrogen and phosphorus are the restrictive factors for the growt...The Hydrobios in Dongping Lake, Shandong Province, mainly includes phytoplankton, zooplankton, benthic animals, fishes and hydrophytic vascular plants. Nitrogen and phosphorus are the restrictive factors for the growth and propagation of hydrophyta, therefore the key to the prevention and control of eutrophication in lakes lies in the control of the contents of the two elements in the water. Artificial fishing of algae can reduce the concentrations of trophic substances such as nitrogen and phosphorus in the water bodies, and biological measures may decrease the contents of these trophic substances in the bottom sediments and the water bodies, thereby playing an active role in modifying the eutrophication of the lake.展开更多
First Ni(Ⅱ)ternary complex using the quinolone antibacterial agent enoxacin(HEn)as ligand and 1,10-phenanthroline as co-ligand has been synthesized and characterized.It is a mononuclear structure,in which enoxacin ac...First Ni(Ⅱ)ternary complex using the quinolone antibacterial agent enoxacin(HEn)as ligand and 1,10-phenanthroline as co-ligand has been synthesized and characterized.It is a mononuclear structure,in which enoxacin acts as a bidentate ligand bound to the metal through the ketone oxygen and a carboxylate oxygen atom.The complex exhibited good binding propensity to human and bovine serum albumin proteins having relatively high binding constants(6.40×10^(4) and 7.12×10^(4),respectively).The investigation of the interaction of the complex with calf-thymus(CT)DNA has been performed with UV and circular dichroism(CD)spectroscopies,indicating that they bind to CT DNA probably by the intercalative binding mode.The binding constant(K_(b))of the complex with CT DNA calculated with UV is 2.03×10^(5),which is higher than that of free enoxacin drug(2.09×10^(4))and even higher than that of typical intercalation indicator(1.23×10^(5))of ethidium bromide(EB).Fluorescence competitive studies with EB have revealed that the complex exhibited the ability to displace the DNA-bound EB using the intercalative binding site.In addition,the antimicrobial activity showed that the complex exhibited a little bit good inhibition(MIC=1.843μg•mL^(-1))against B.subtilis than free HEn.展开更多
Konjac glucomannan(KGM)is recognized as a safe material for its health-promoting benefits and thus widely used in various fields including pharmaceutical industry.In recent decades,the combination of collagen and KGM ...Konjac glucomannan(KGM)is recognized as a safe material for its health-promoting benefits and thus widely used in various fields including pharmaceutical industry.In recent decades,the combination of collagen and KGM attracts more attentions for biomedical purpose,especially the hybrid films of collagen-KGM or collagen-KGM-polysaccharide.In this study,to further and deeply develop the intrinsic values of both collagen and KGM as biomaterials,a novel kind of composite hydrogel comprising collagen and KGM at a certain ratio was fabricated under mild conditions via fibrillogenesis process of the aqueous blends of collagen and KGM that experienced deacetylation simultaneously.The chemical composition,microcosmic architectures,swelling behavior,biodegradation and dynamic mechanic properties of such resulted composite hydrogels were systematically investigated.Biologic experiments,including cell culture in vitro and hypodermic implantation in vivo,were also conducted on these collagen/KGM composite hydrogels to evaluate their biologic performances.The relevant results prove that,based on collagen self-assembly behavior,this synthesis strategy is efficient to construct a composite hydrogel of collagen/KGM with improved mechanical properties,biodegradability,excellent biocompatibility and bioactivity,which are promising for potential biomedical applications such as tissue engineering and regenerative medicine.展开更多
文摘Taking the water ecosystem of Small Xingkai Lake as research object,through the survey and analysis of aquatic organism indicators,this paper established an ecosystem integrity evaluation system with the biological integrity as the criterion layer. Using the index of biological integrity and comprehensive health index,it evaluated the health status of water ecosystem of Small Xingkai Lake.
基金This work was supported by the Fundamental Research Funds for the Central Universities(Nos.31920170193 and 31920200026).
文摘Dysregulated HGF/c-Met signalling has been associated with many human cancers,poor clinical outcomes,and even resistance acquisition to some approved targeted therapies.As such,c-Met kinase has emerged as an attractive target for anticancer drug discovery.
文摘The Hydrobios in Dongping Lake, Shandong Province, mainly includes phytoplankton, zooplankton, benthic animals, fishes and hydrophytic vascular plants. Nitrogen and phosphorus are the restrictive factors for the growth and propagation of hydrophyta, therefore the key to the prevention and control of eutrophication in lakes lies in the control of the contents of the two elements in the water. Artificial fishing of algae can reduce the concentrations of trophic substances such as nitrogen and phosphorus in the water bodies, and biological measures may decrease the contents of these trophic substances in the bottom sediments and the water bodies, thereby playing an active role in modifying the eutrophication of the lake.
基金The authors thank the financial support by Jiangsu National Science Foundation(No.BK2009239)The project was also funded by the Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources(Guangxi Normal University)the Ministry of Education of China(No.CMEMR2013-B02).
文摘First Ni(Ⅱ)ternary complex using the quinolone antibacterial agent enoxacin(HEn)as ligand and 1,10-phenanthroline as co-ligand has been synthesized and characterized.It is a mononuclear structure,in which enoxacin acts as a bidentate ligand bound to the metal through the ketone oxygen and a carboxylate oxygen atom.The complex exhibited good binding propensity to human and bovine serum albumin proteins having relatively high binding constants(6.40×10^(4) and 7.12×10^(4),respectively).The investigation of the interaction of the complex with calf-thymus(CT)DNA has been performed with UV and circular dichroism(CD)spectroscopies,indicating that they bind to CT DNA probably by the intercalative binding mode.The binding constant(K_(b))of the complex with CT DNA calculated with UV is 2.03×10^(5),which is higher than that of free enoxacin drug(2.09×10^(4))and even higher than that of typical intercalation indicator(1.23×10^(5))of ethidium bromide(EB).Fluorescence competitive studies with EB have revealed that the complex exhibited the ability to displace the DNA-bound EB using the intercalative binding site.In addition,the antimicrobial activity showed that the complex exhibited a little bit good inhibition(MIC=1.843μg•mL^(-1))against B.subtilis than free HEn.
基金The present study was supported by the National Natural Science Foundation of China(Grants 51373105 and 51203103)Sichuan Province Key Research and Development Project(2018SZ0046).
文摘Konjac glucomannan(KGM)is recognized as a safe material for its health-promoting benefits and thus widely used in various fields including pharmaceutical industry.In recent decades,the combination of collagen and KGM attracts more attentions for biomedical purpose,especially the hybrid films of collagen-KGM or collagen-KGM-polysaccharide.In this study,to further and deeply develop the intrinsic values of both collagen and KGM as biomaterials,a novel kind of composite hydrogel comprising collagen and KGM at a certain ratio was fabricated under mild conditions via fibrillogenesis process of the aqueous blends of collagen and KGM that experienced deacetylation simultaneously.The chemical composition,microcosmic architectures,swelling behavior,biodegradation and dynamic mechanic properties of such resulted composite hydrogels were systematically investigated.Biologic experiments,including cell culture in vitro and hypodermic implantation in vivo,were also conducted on these collagen/KGM composite hydrogels to evaluate their biologic performances.The relevant results prove that,based on collagen self-assembly behavior,this synthesis strategy is efficient to construct a composite hydrogel of collagen/KGM with improved mechanical properties,biodegradability,excellent biocompatibility and bioactivity,which are promising for potential biomedical applications such as tissue engineering and regenerative medicine.